Restriction map for
pFN21AE3315
(Circular) (Six-base) MAPSORT of: pFN21AE3315.seq Check: 1418 from: 1 to: 7288
pFN21AE3315 7288 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4135 4135
Size: 7288
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5098 278
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4631 1937
Size: 2694 4594
Acc65I G'GTAC_C
Cuts at: 2252 2461 3280 4180 4609 2252
Size: 209 819 900 429 4931
Fragments arranged by size:
4931 900 819 429 209
AclI AA'CG_TT
Cuts at: 1563 5415 5788 1563
Size: 3852 373 3063
Fragments arranged by size:
3852 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2984 3228 3295 4193 5351 6735 7003 1632
Size: 1352 244 67 898 1158 1384 268 1917
Fragments arranged by size:
1917 1384 1352 1158 898 268 244 67
AfeI AGC'GCT
Cuts at: 2121 2121
Size: 7288
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7091
AflIII A'CryG_T
Cuts at: 1203 2775 3756 6187 7164 1203
Size: 1572 981 2431 977 1327
Fragments arranged by size:
2431 1572 1327 981 977
AgeI A'CCGG_T
Cuts at: 6119 6119
Size: 7288
AhdI GACnn_n'nnGTC
Cuts at: 6017 6017
Size: 7288
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7288
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4412 1184
Size: 3228 4060
AlwNI CAG_nnn'CTG
Cuts at: 2053 2612 3382 5031 6603 2053
Size: 559 770 1649 1572 2738
Fragments arranged by size:
2738 1649 1572 770 559
ApaI G_GGCC'C
Cuts at: 2268 2268
Size: 7288
ApaLI G'TGCA_C
Cuts at: 5345 6501 5345
Size: 1156 6132
ApoI r'AATT_y
Cuts at: 1493 3596 4597 4823 4975 7084 1493
Size: 2103 1001 226 152 2109 1697
Fragments arranged by size:
2109 2103 1697 1001 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5842 160
Size: 872 4810 1606
Fragments arranged by size:
4810 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 3471 3750 4457 4613 1870
Size: 73 77 1451 279 707 156 4545
Fragments arranged by size:
4545 1451 707 279 156 77 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3678 3711 3678
Size: 33 7255
BamHI G'GATC_C
Cuts at: 4240 4618 4985 4240
Size: 378 367 6543
Fragments arranged by size:
6543 378 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2231 2252 2306 2461 2867
Size: 325 217 627 444 21 54 155 406
Cuts at: 2867 3197 3280 3305 4180 4609 6064 618
Size: 330 83 25 875 429 1455 1842
Fragments arranged by size:
1842 1455 875 627 444 429 406 330
325 217 155 83 54 25 21
BanII G_rGCy'C
Cuts at: 1391 2268 2298 2355 2455 4577 4607 1391
Size: 877 30 57 100 2122 30 4072
Fragments arranged by size:
4072 2122 877 100 57 30 30
BbeI G_GCGC'C
Cuts at: 2235 2310 2235
Size: 75 7213
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3490 4455 928
Size: 971 1591 965 3761
Fragments arranged by size:
3761 1591 971 965
BbvCI CC'TCA_GC
Cuts at: 2586 2586
Size: 7288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5479 5513 1903
Size: 34 3542 34 3678
Fragments arranged by size:
3678 3542 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2893 3679 5180 6390 2893
Size: 786 1501 1210 3791
Fragments arranged by size:
3791 1501 1210 786
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7288
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3195 5899 136
Size: 107 122 71 631 605 1523 2704 1525
Fragments arranged by size:
2704 1525 1523 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7283 7283
Size: 7288
BlpI GC'TnA_GC
Cuts at: 3679 4705 3679
Size: 1026 6262
Bme1580I G_kGCm'C
Cuts at: 1790 2268 2870 3308 3674 3699 5349 6505 1790
Size: 478 602 438 366 25 1650 1156 2573
Fragments arranged by size:
2573 1650 1156 602 478 438 366 25
BmgBI CAC'GTC
Cuts at: 1318 2980 1318
Size: 1662 5626
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2821 2824 2851
Size: 416 53 83 399 24 1404 3 27
Cuts at: 2851 3311 5019 5977 442
Size: 460 1708 958 1753
Fragments arranged by size:
1753 1708 1404 958 460 416 399 83
53 27 24 3
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2162 3248 2162
Size: 1086 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2471 3041 3925 4459 5948 1239
Size: 815 417 570 884 534 1489 2579
Fragments arranged by size:
2579 1489 884 815 570 534 417
Bpu10I CC'TnA_GC
Cuts at: 2531 2586 2531
Size: 55 7233
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3506 5413 6278 6576 3506
Size: 1907 865 298 4218
Fragments arranged by size:
4218 1907 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2575 2987 3468 4433 5951 882
Size: 617 1076 412 481 965 1518 2219
Fragments arranged by size:
2219 1518 1076 965 617 481 412
BsaAI yAC'GTr
Cuts at: 493 1204 5079 493
Size: 711 3875 2702
Fragments arranged by size:
3875 2702 711
BsaBI GATnn'nnATC
Cuts at: 3059 4412 7282 3059
Size: 1353 2870 3065
Fragments arranged by size:
3065 2870 1353
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2232 2307 5095 5477
Size: 53 83 186 936 699 75 2788 382
Cuts at: 5477 6112 275
Size: 635 1451
Fragments arranged by size:
2788 1451 936 699 635 382 186 83
75 53
BsaWI w'CCGG_w
Cuts at: 4356 5721 6119 6393 6540 6951 4356
Size: 1365 398 274 147 411 4693
Fragments arranged by size:
4693 1365 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2738 2768 1178
Size: 30 165 30 1335 30 5698
Fragments arranged by size:
5698 1335 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2345 2780 3908 4145 4535 4598 1186
Size: 1159 435 1128 237 390 63 3876
Fragments arranged by size:
3876 1159 1128 435 390 237 63
BseYI C'CCAG_C
Cuts at: 2053 2167 2242 3407 3919 4429 6491 6987 2053
Size: 114 75 1165 512 510 2062 496 2354
Fragments arranged by size:
2354 2062 1165 512 510 496 114 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3254 4083 4334 1163
Size: 573 1518 829 251 4117
Fragments arranged by size:
4117 1518 829 573 251
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2108 2525 4738 5499 6527 1540
Size: 275 186 107 417 2213 761 1028 2301
Fragments arranged by size:
2301 2213 1028 761 417 275 186 107
BsiHKAI G_wGCw'C
Cuts at: 2148 2298 2391 2532 5349 5434 6505 2148
Size: 150 93 141 2817 85 1071 2931
Fragments arranged by size:
2931 2817 1071 150 141 93 85
BsmI GAATG_Cn'
Cuts at: 3151 3496 4799 4892 3151
Size: 345 1303 93 5547
Fragments arranged by size:
5547 1303 345 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3101 3971 3101
Size: 870 6418
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2148 2268 2298 2355 2391 2455 2532
Size: 399 358 120 30 57 36 64 77
Cuts at: 2532 2870 3308 3674 3699 4577 4607 5349 5434
Size: 338 438 366 25 878 30 742 85
Cuts at: 5434 6505 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 878 742 438 399 366 358
338 120 85 77 64 57 36 30
30 25
BspEI T'CCGG_A
Cuts at: 6951 6951
Size: 7288
BspHI T'CATG_A
Cuts at: 5177 6907 5177
Size: 1730 5558
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3292 4135 4154 4643 844
Size: 2448 843 19 489 3489
Fragments arranged by size:
3489 2448 843 489 19
BsrBI CCG'CTC
Cuts at: 1394 1823 5175 1394
Size: 429 3352 3507
Fragments arranged by size:
3507 3352 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2347 2803 3739 5783 5957 65
Size: 1183 1099 456 936 2044 174 1396
Fragments arranged by size:
2044 1396 1183 1099 936 456 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2194 2255 2524 2590 5932 6119 1700
Size: 110 384 61 269 66 3342 187 2869
Fragments arranged by size:
3342 2869 384 269 187 110 66 61
BsrGI T'GTAC_A
Cuts at: 96 2614 96
Size: 2518 4770
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7288
BssSI C'ACGA_G
Cuts at: 5348 6360 5348
Size: 1012 6276
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7288
BstBI TT'CG_AA
Cuts at: 6092 6092
Size: 7288
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7288
BstXI CCAn_nnnn'nTGG
Cuts at: 3629 3734 3629
Size: 105 7183
BstYI r'GATC_y
Cuts at: 1972 3054 3252 4240 4407 4618 4985 5370 5387
Size: 1082 198 988 167 211 367 385 17
Cuts at: 5387 6839 6925 6937 7283 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1082 988 385 367 346 211
198 167 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2322 2347 3447 513
Size: 552 939 318 25 1100 4354
Fragments arranged by size:
4354 1100 939 552 318 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2552 2835 487
Size: 683 56 1326 283 4940
Fragments arranged by size:
4940 1326 683 283 56
BtsI GCAGTG_nn'
Cuts at: 4804 5598 5618 4804
Size: 794 20 6474
Fragments arranged by size:
6474 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7288
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2913 2948 529
Size: 35 2349 35 4869
Fragments arranged by size:
4869 2349 35 35
DraI TTT'AAA
Cuts at: 4592 4592
Size: 7288
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3032 6295 809
Size: 507 1716 3263 1802
Fragments arranged by size:
3263 1802 1716 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2105 2344 2522
Size: 54 1425 50 275 18 275 239 178
Cuts at: 2522 3359 4554 4735 5624 7253 8
Size: 837 1195 181 889 1629 43
Fragments arranged by size:
1629 1425 1195 889 837 275 275 239
181 178 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2105 2522 4735 1537
Size: 275 293 417 2213 4090
Fragments arranged by size:
4090 2213 417 293 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3657 4490 5218 1353
Size: 2304 833 728 3423
Fragments arranged by size:
3423 2304 833 728
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2456 2463 5875 6249 6395 387
Size: 1064 138 867 7 3412 374 146 1280
Fragments arranged by size:
3412 1280 1064 867 374 146 138 7
EcoICRI GAG'CTC
Cuts at: 2296 2296
Size: 7288
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2471 2984 3041 3228 3295 3925
Size: 393 422 417 513 57 187 67 630
Cuts at: 3925 4193 4459 5351 5948 6735 7003 1239
Size: 268 266 892 597 787 268 1524
Fragments arranged by size:
1524 892 787 630 597 513 422 417
393 268 268 266 187 67 57
EcoNI CCTnn'n_nnAGG
Cuts at: 2696 2696
Size: 7288
EcoO109I rG'GnC_Cy
Cuts at: 2012 2164 2212 2265 2665 2826 2012
Size: 152 48 53 400 161 6474
Fragments arranged by size:
6474 400 161 152 53 48
EcoRI G'AATT_C
Cuts at: 4597 4975 4597
Size: 378 6910
EcoRV GAT'ATC
Cuts at: 3182 3182
Size: 7288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3427 3427
Size: 7288
FspI TGC'GCA
Cuts at: 2318 4268 5794 2318
Size: 1950 1526 3812
Fragments arranged by size:
3812 1950 1526
FspAI rTGC'GCAy
Cuts at: 2318 2318
Size: 7288
HaeII r_GCGC'y
Cuts at: 1678 2123 2235 2310 6435 1678
Size: 445 112 75 4125 2531
Fragments arranged by size:
4125 2531 445 112 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2738 2770 5929 5961 6003
Size: 32 135 32 1166 32 3159 32 42
Cuts at: 6003 6035 1373
Size: 32 2626
Fragments arranged by size:
3159 2626 1166 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4632 4878 669
Size: 988 281 2694 246 3079
Fragments arranged by size:
3079 2694 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2153 748
Size: 1405 5883
HpaI GTT'AAC
Cuts at: 4878 4878
Size: 7288
KasI G'GCGC_C
Cuts at: 2231 2306 2231
Size: 75 7213
KpnI G_GTAC'C
Cuts at: 2256 2465 3284 4184 4613 2256
Size: 209 819 900 429 4931
Fragments arranged by size:
4931 900 819 429 209
MfeI C'AATT_G
Cuts at: 4887 4887
Size: 7288
MluI A'CGCG_T
Cuts at: 7164 7164
Size: 7288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4129 4200 6402 6586 1305
Size: 2824 71 2202 184 2007
Fragments arranged by size:
2824 2202 2007 184 71
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2346 4556 10
Size: 54 1425 343 514 2210 2742
Fragments arranged by size:
2742 2210 1425 514 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4073 4568 5246 5605 5764 518
Size: 648 2907 495 678 359 159 2042
Fragments arranged by size:
2907 2042 678 648 495 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2110 2171 2380 2491 3130 3358 3683
Size: 444 60 61 209 111 639 228 325
Cuts at: 3683 3814 3923 4704 5381 6529 6774 1606
Size: 131 109 781 677 1148 245 2120
Fragments arranged by size:
2120 1148 781 677 639 444 325 245
228 209 131 111 109 61 60
NaeI GCC'GGC
Cuts at: 2592 2592
Size: 7288
NarI GG'CG_CC
Cuts at: 2232 2307 2232
Size: 75 7213
NcoI C'CATG_G
Cuts at: 513 1065 2004 3447 513
Size: 552 939 1443 4354
Fragments arranged by size:
4354 1443 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7288
NgoMIV G'CCGG_C
Cuts at: 2590 2590
Size: 7288
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7288
NotI GC'GGCC_GC
Cuts at: 2105 4735 2105
Size: 2630 4658
NspI r_CATG'y
Cuts at: 1132 1229 3760 4646 6191 1132
Size: 97 2531 886 1545 2229
Fragments arranged by size:
2531 2229 1545 886 97
PasI CC'CwG_GG
Cuts at: 1348 4055 1348
Size: 2707 4581
PciI A'CATG_T
Cuts at: 3756 6187 3756
Size: 2431 4857
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3301 4039 1108
Size: 2193 738 4357
Fragments arranged by size:
4357 2193 738
PfoI T'CCnGG_A
Cuts at: 3018 3636 3948 3018
Size: 618 312 6358
Fragments arranged by size:
6358 618 312
PmeI GTTT'AAAC
Cuts at: 4592 4592
Size: 7288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5357 5389 6870 6902 5357
Size: 32 1481 32 5743
Fragments arranged by size:
5743 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2012 2164 2665 2826 2012
Size: 152 501 161 6474
Fragments arranged by size:
6474 501 161 152
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7288
PsiI TTA'TAA
Cuts at: 4858 4858
Size: 7288
PspOMI G'GGCC_C
Cuts at: 2264 2264
Size: 7288
PstI C_TGCA'G
Cuts at: 830 2412 3240 4640 830
Size: 1582 828 1400 3478
Fragments arranged by size:
3478 1582 1400 828
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7288
PvuII CAG'CTG
Cuts at: 2050 2380 3814 3923 2050
Size: 330 1434 109 5415
Fragments arranged by size:
5415 1434 330 109
SacI G_AGCT'C
Cuts at: 2298 2298
Size: 7288
SalI G'TCGA_C
Cuts at: 1936 4630 1936
Size: 2694 4594
SanDI GG'GwC_CC
Cuts at: 2826 2826
Size: 7288
SapI GCTCTTCn'nnn_
Cuts at: 3657 3657
Size: 7288
SbfI CC_TGCA'GG
Cuts at: 4640 4640
Size: 7288
ScaI AGT'ACT
Cuts at: 3213 5536 7248 3213
Size: 2323 1712 3253
Fragments arranged by size:
3253 2323 1712
SexAI A'CCwGG_T
Cuts at: 3276 3276
Size: 7288
SfcI C'TryA_G
Cuts at: 826 1045 2408 3236 3556 4636 5771 6452 6643
Size: 219 1363 828 320 1080 1135 681 191
Cuts at: 6643 826
Size: 1471
Fragments arranged by size:
1471 1363 1135 1080 828 681 320 219
191
SfoI GGC'GCC
Cuts at: 2233 2308 2233
Size: 75 7213
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7288
SmaI CCC'GGG
Cuts at: 1872 2022 4615 1872
Size: 150 2593 4545
Fragments arranged by size:
4545 2593 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3471 3521 5392 6293 6555 820
Size: 197 926 1528 50 1871 901 262 1553
Fragments arranged by size:
1871 1553 1528 926 901 262 197 50
SnaBI TAC'GTA
Cuts at: 493 5079 493
Size: 4586 2702
SpeI A'CTAG_T
Cuts at: 152 3167 152
Size: 3015 4273
SphI G_CATG'C
Cuts at: 1132 4646 1132
Size: 3514 3774
SrfI GCCC'GGGC
Cuts at: 2022 2022
Size: 7288
SspI AAT'ATT
Cuts at: 5 52 5212 5
Size: 47 5160 2081
Fragments arranged by size:
5160 2081 47
StuI AGG'CCT
Cuts at: 1517 3366 3519 1517
Size: 1849 153 5286
Fragments arranged by size:
5286 1849 153
StyI C'CwwG_G
Cuts at: 513 1065 2004 3447 3622 4509 4577 4727 513
Size: 552 939 1443 175 887 68 150 3074
Fragments arranged by size:
3074 1443 939 887 552 175 150 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2347 3518 5315 5332 5485 5670 6115
Size: 1255 767 1171 1797 17 153 185 445
Cuts at: 6115 325
Size: 1498
Fragments arranged by size:
1797 1498 1255 1171 767 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2614 3211 4021
Size: 274 80 33 51 1443 637 597 810
Cuts at: 4021 5534 7246 96
Size: 1513 1712 138
Fragments arranged by size:
1712 1513 1443 810 637 597 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3545 3577 7248 7280 3545
Size: 32 3671 32 3553
Fragments arranged by size:
3671 3553 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7288
XbaI T'CTAG_A
Cuts at: 3122 4624 3122
Size: 1502 5786
XhoI C'TCGA_G
Cuts at: 1943 3471 1943
Size: 1528 5760
XmaI C'CCGG_G
Cuts at: 1870 2020 4613 1870
Size: 150 2593 4545
Fragments arranged by size:
4545 2593 150
XmnI GAAnn'nnTTC
Cuts at: 3335 4340 5417 3335
Size: 1005 1077 5206
Fragments arranged by size:
5206 1077 1005
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5096 276
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstZ17I Bsu36I DraIII
FseI NruI NsiI PacI PmlI PspXI PsrI RsrII
SacII SfiI SwaI XcmI