Restriction map for
pFN21AE3314
(Circular) (Six-base) MAPSORT of: pFN21AE3314.seq Check: 4527 from: 1 to: 7270
pFN21AE3314 7270 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2985 5080 278
Size: 53 83 186 936 1449 2095 2468
Fragments arranged by size:
2468 2095 1449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4613 1937
Size: 2676 4594
Acc65I G'GTAC_C
Cuts at: 4591 4591
Size: 7270
AclI AA'CG_TT
Cuts at: 1563 5397 5770 1563
Size: 3834 373 3063
Fragments arranged by size:
3834 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2273 2949 3147 4085 5333 6717 6985 1632
Size: 641 676 198 938 1248 1384 268 1917
Fragments arranged by size:
1917 1384 1248 938 676 641 268 198
AflII C'TTAA_G
Cuts at: 820 1017 3872 820
Size: 197 2855 4218
Fragments arranged by size:
4218 2855 197
AflIII A'CryG_T
Cuts at: 1203 2751 2874 6169 7146 1203
Size: 1548 123 3295 977 1327
Fragments arranged by size:
3295 1548 1327 977 123
AgeI A'CCGG_T
Cuts at: 6101 6101
Size: 7270
AhdI GACnn_n'nnGTC
Cuts at: 5999 5999
Size: 7270
AleI CACnn'nnGTG
Cuts at: 1166 2300 1166
Size: 1134 6136
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7270
AlwNI CAG_nnn'CTG
Cuts at: 2425 3107 3575 3626 4497 5013 6585 2425
Size: 682 468 51 871 516 1572 3110
Fragments arranged by size:
3110 1572 871 682 516 468 51
ApaLI G'TGCA_C
Cuts at: 5327 6483 5327
Size: 1156 6114
ApoI r'AATT_y
Cuts at: 1493 2825 2842 3482 3764 4579 4805 4957 7066
Size: 1332 17 640 282 815 226 152 2109
Cuts at: 7066 1493
Size: 1697
Fragments arranged by size:
2109 1697 1332 815 640 282 226 152
17
AseI AT'TA_AT
Cuts at: 160 1032 5824 160
Size: 872 4792 1606
Fragments arranged by size:
4792 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7270
AvaI C'yCGr_G
Cuts at: 1870 1943 4595 1870
Size: 73 2652 4545
Fragments arranged by size:
4545 2652 73
BamHI G'GATC_C
Cuts at: 3201 4230 4600 4967 3201
Size: 1029 370 367 5504
Fragments arranged by size:
5504 1029 370 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2220 2316 3010 3151 3556
Size: 325 217 627 433 96 694 141 405
Cuts at: 3556 4591 6046 618
Size: 1035 1455 1842
Fragments arranged by size:
1842 1455 1035 694 627 433 405 325
217 141 96
BanII G_rGCy'C
Cuts at: 1391 4017 4589 1391
Size: 2626 572 4072
Fragments arranged by size:
4072 2626 572
BbeI G_GCGC'C
Cuts at: 2320 2320
Size: 7270
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6299
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5461 5495 1903
Size: 34 3524 34 3678
Fragments arranged by size:
3678 3524 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2663 5162 6372 2663
Size: 2499 1210 3561
Fragments arranged by size:
3561 2499 1210
BclI T'GATC_A
Cuts at: 1548 3960 1548
Size: 2412 4858
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3460 5881 136
Size: 107 122 71 631 605 1788 2421 1525
Fragments arranged by size:
2421 1788 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2895 3985 4132 7265 2895
Size: 1090 147 3133 2900
Fragments arranged by size:
3133 2900 1090 147
BlpI GC'TnA_GC
Cuts at: 4490 4687 4490
Size: 197 7073
Bme1580I G_kGCm'C
Cuts at: 1790 2225 3561 5331 6487 1790
Size: 435 1336 1770 1156 2573
Fragments arranged by size:
2573 1770 1336 1156 435
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7270
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2985 3534 5001
Size: 416 53 83 399 24 1568 549 1467
Cuts at: 5001 5959 442
Size: 958 1753
Fragments arranged by size:
1753 1568 1467 958 549 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2577 2640 3286 3311 3474 4015 4428 4519
Size: 1338 63 646 25 163 541 413 91
Cuts at: 4519 5930 1239
Size: 1411 2579
Fragments arranged by size:
2579 1411 1338 646 541 413 163 91
63 25
Bpu10I CC'TnA_GC
Cuts at: 2073 2073
Size: 7270
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2077 3884 5395 6260 6558 2077
Size: 1807 1511 865 298 2789
Fragments arranged by size:
2789 1807 1511 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4416 5933 882
Size: 617 2917 1517 2219
Fragments arranged by size:
2917 2219 1517 617
BsaAI yAC'GTr
Cuts at: 493 1204 2752 5061 493
Size: 711 1548 2309 2702
Fragments arranged by size:
2702 2309 1548 711
BsaBI GATnn'nnATC
Cuts at: 3884 3990 7264 3884
Size: 106 3274 3890
Fragments arranged by size:
3890 3274 106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2317 2982 5077 5459
Size: 53 83 186 936 784 665 2095 382
Cuts at: 5459 6094 275
Size: 635 1451
Fragments arranged by size:
2095 1451 936 784 665 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2496 4227 5703 6101 6375 6522 6933 2496
Size: 1731 1476 398 274 147 411 2833
Fragments arranged by size:
2833 1731 1476 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2897 2927 3459 3488 3489
Size: 30 165 30 1494 30 532 29 1
Cuts at: 3489 3518 1178
Size: 29 4930
Fragments arranged by size:
4930 1494 532 165 30 30 30 29
29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3370 3512 3515 3686 1186
Size: 2184 142 3 171 4770
Fragments arranged by size:
4770 2184 171 142 3
BseYI C'CCAG_C
Cuts at: 2597 3709 4285 4374 6473 6969 2597
Size: 1112 576 89 2099 496 2898
Fragments arranged by size:
2898 2099 1112 576 496 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6697
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4720 5481 6509 1540
Size: 275 186 1420 1299 761 1028 2301
Fragments arranged by size:
2301 1420 1299 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2998 4251 5331 5416 6487 2998
Size: 1253 1080 85 1071 3781
Fragments arranged by size:
3781 1253 1080 1071 85
BsmI GAATG_Cn'
Cuts at: 2028 3859 4781 4874 2028
Size: 1831 922 93 4424
Fragments arranged by size:
4424 1831 922 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2225 2998 3561 4017 4251 4589 5331
Size: 399 435 773 563 456 234 338 742
Cuts at: 5331 5416 6487 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 773 742 563 456 435 399
338 234 85
BspEI T'CCGG_A
Cuts at: 6933 6933
Size: 7270
BspHI T'CATG_A
Cuts at: 5159 6889 5159
Size: 1730 5540
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3651 4625 844
Size: 2807 974 3489
Fragments arranged by size:
3489 2807 974
BsrBI CCG'CTC
Cuts at: 1394 1823 2925 3378 5157 1394
Size: 429 1102 453 1779 3507
Fragments arranged by size:
3507 1779 1102 453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3117 5765 5939 65
Size: 1183 1869 2648 174 1396
Fragments arranged by size:
2648 1869 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5914 6101 1700
Size: 110 4104 187 2869
Fragments arranged by size:
4104 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2355 3114 4209 96
Size: 2259 759 1095 3157
Fragments arranged by size:
3157 2259 1095 759
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7270
BssSI C'ACGA_G
Cuts at: 4010 5330 6342 4010
Size: 1320 1012 4938
Fragments arranged by size:
4938 1320 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3286 794
Size: 2492 4778
BstBI TT'CG_AA
Cuts at: 6074 6074
Size: 7270
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7270
BstXI CCAn_nnnn'nTGG
Cuts at: 3542 3542
Size: 7270
BstYI r'GATC_y
Cuts at: 1972 2391 2895 3201 3985 4132 4230 4600 4967
Size: 419 504 306 784 147 98 370 367
Cuts at: 4967 5352 5369 6821 6907 6919 7265 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 784 504 419 385 370 367
346 306 147 98 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3549 3549
Size: 7270
BtgI C'CryG_G
Cuts at: 513 1065 3636 513
Size: 552 2571 4147
Fragments arranged by size:
4147 2571 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4050 487
Size: 683 56 2824 3707
Fragments arranged by size:
3707 2824 683 56
BtsI GCAGTG_nn'
Cuts at: 2863 4251 4786 5580 5600 2863
Size: 1388 535 794 20 4533
Fragments arranged by size:
4533 1388 794 535 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7235
DraI TTT'AAA
Cuts at: 4574 4574
Size: 7270
DraIII CAC_nnn'GTG
Cuts at: 3392 3392
Size: 7270
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6277 809
Size: 507 4961 1802
Fragments arranged by size:
4961 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3030 3222 3243
Size: 54 1425 50 275 18 1200 192 21
Cuts at: 3243 4137 4717 5606 7235 8
Size: 894 580 889 1629 43
Fragments arranged by size:
1629 1425 1200 894 889 580 275 192
54 50 43 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 4717 1537
Size: 275 2905 4090
Fragments arranged by size:
4090 2905 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4498 5200 1353
Size: 3145 702 3423
Fragments arranged by size:
3423 3145 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2416 2797 5857 6231 6377 387
Size: 1064 138 827 381 3060 374 146 1280
Fragments arranged by size:
3060 1280 1064 827 381 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2273 2577 2640 2949 3147 3286 3311
Size: 393 641 304 63 309 198 139 25
Cuts at: 3311 3474 4015 4085 4428 4519 5333 5930 6717
Size: 163 541 70 343 91 814 597 787
Cuts at: 6717 6985 1239
Size: 268 1524
Fragments arranged by size:
1524 814 787 641 597 541 393 343
309 304 268 198 163 139 91 70
63 25
EcoO109I rG'GnC_Cy
Cuts at: 2307 3904 2307
Size: 1597 5673
EcoRI G'AATT_C
Cuts at: 4579 4957 4579
Size: 378 6892
FspI TGC'GCA
Cuts at: 5776 5776
Size: 7270
HaeII r_GCGC'y
Cuts at: 1678 2320 6417 1678
Size: 642 4097 2531
Fragments arranged by size:
4097 2531 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2613 2645 2696 2728 3403
Size: 32 135 32 1041 32 51 32 675
Cuts at: 3403 3435 3488 3520 3632 3664 3877 3909 5911
Size: 32 53 32 112 32 213 32 2002
Cuts at: 5911 5943 5985 6017 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2002 1041 675 213 135 112 53
51 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2986 4614 4860 669
Size: 988 281 1048 1628 246 3079
Fragments arranged by size:
3079 1628 1048 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7270
HpaI GTT'AAC
Cuts at: 4860 4860
Size: 7270
KasI G'GCGC_C
Cuts at: 2316 2316
Size: 7270
KpnI G_GTAC'C
Cuts at: 4595 4595
Size: 7270
MfeI C'AATT_G
Cuts at: 2696 4869 2696
Size: 2173 5097
MluI A'CGCG_T
Cuts at: 7146 7146
Size: 7270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2647 3429 6384 6568 1305
Size: 1342 782 2955 184 2007
Fragments arranged by size:
2955 2007 1342 782 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3032 3224 3245 4139 10
Size: 54 1425 343 1200 192 21 894 3141
Fragments arranged by size:
3141 1425 1200 894 343 192 54 21
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 2528 2852 3741 5228 5587 5746
Size: 648 1134 228 324 889 1487 359 159
Cuts at: 5746 518
Size: 2042
Fragments arranged by size:
2042 1487 1134 889 648 359 324 228
159
MspA1I CmG'CkG
Cuts at: 1606 2174 2597 3516 4260 4378 4494 4686 5363
Size: 568 423 919 744 118 116 192 677
Cuts at: 5363 6511 6756 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 919 744 677 568 423 245
192 118 116
NarI GG'CG_CC
Cuts at: 2317 2317
Size: 7270
NcoI C'CATG_G
Cuts at: 513 1065 3636 513
Size: 552 2571 4147
Fragments arranged by size:
4147 2571 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7270
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7270
NotI GC'GGCC_GC
Cuts at: 4717 4717
Size: 7270
NspI r_CATG'y
Cuts at: 1132 1229 2113 2878 3364 4628 6173 1132
Size: 97 884 765 486 1264 1545 2229
Fragments arranged by size:
2229 1545 1264 884 765 486 97
PasI CC'CwG_GG
Cuts at: 1348 2311 1348
Size: 963 6307
PciI A'CATG_T
Cuts at: 2874 6169 2874
Size: 3295 3975
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2157 2940 3028 1108
Size: 1049 783 88 5350
Fragments arranged by size:
5350 1049 783 88
PfoI T'CCnGG_A
Cuts at: 2973 2973
Size: 7270
PmeI GTTT'AAAC
Cuts at: 4574 4574
Size: 7270
PmlI CAC'GTG
Cuts at: 2752 2752
Size: 7270
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4508 4540 5339 5371 6852 6884 4508
Size: 32 799 32 1481 32 4894
Fragments arranged by size:
4894 1481 799 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2307 3904 2307
Size: 1597 5673
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7270
PsiI TTA'TAA
Cuts at: 4840 4840
Size: 7270
PstI C_TGCA'G
Cuts at: 830 2268 3515 4622 830
Size: 1438 1247 1107 3478
Fragments arranged by size:
3478 1438 1247 1107
PvuI CG_AT'CG
Cuts at: 2001 3421 2001
Size: 1420 5850
PvuII CAG'CTG
Cuts at: 2174 2597 4378 4494 2174
Size: 423 1781 116 4950
Fragments arranged by size:
4950 1781 423 116
SalI G'TCGA_C
Cuts at: 1936 4612 1936
Size: 2676 4594
SanDI GG'GwC_CC
Cuts at: 2307 2307
Size: 7270
SbfI CC_TGCA'GG
Cuts at: 4622 4622
Size: 7270
ScaI AGT'ACT
Cuts at: 5518 7230 5518
Size: 1712 5558
SfcI C'TryA_G
Cuts at: 826 1045 2264 3511 4299 4618 5753 6434 6625
Size: 219 1219 1247 788 319 1135 681 191
Cuts at: 6625 826
Size: 1471
Fragments arranged by size:
1471 1247 1219 1135 788 681 319 219
191
SfoI GGC'GCC
Cuts at: 2318 2318
Size: 7270
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7270
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7270
SmaI CCC'GGG
Cuts at: 1872 4597 1872
Size: 2725 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2056 3872 3899 5374 6275 6537
Size: 197 926 113 1816 27 1475 901 262
Cuts at: 6537 820
Size: 1553
Fragments arranged by size:
1816 1553 1475 926 901 262 197 113
27
SnaBI TAC'GTA
Cuts at: 493 5061 493
Size: 4568 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7270
SphI G_CATG'C
Cuts at: 1132 4628 1132
Size: 3496 3774
SspI AAT'ATT
Cuts at: 5 52 5194 5
Size: 47 5142 2081
Fragments arranged by size:
5142 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7270
StyI C'CwwG_G
Cuts at: 513 1065 3592 3636 4426 4709 513
Size: 552 2527 44 790 283 3074
Fragments arranged by size:
3074 2527 790 552 283 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 2747 3169 5297 5314 5467 5652
Size: 1255 1137 30 422 2128 17 153 185
Cuts at: 5652 6097 325
Size: 445 1498
Fragments arranged by size:
2128 1498 1255 1137 445 422 185 153
30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2355 2611 3114
Size: 274 80 33 51 1443 378 256 503
Cuts at: 3114 4209 5516 7228 96
Size: 1095 1307 1712 138
Fragments arranged by size:
1712 1443 1307 1095 503 378 274 256
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2776 2808 2897 2929 3645 3677 7230 7262 2776
Size: 32 89 32 716 32 3553 32 2784
Fragments arranged by size:
3553 2784 716 89 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7270
XbaI T'CTAG_A
Cuts at: 4606 4606
Size: 7270
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2026 2702 3145 4088 2026
Size: 676 443 943 5208
Fragments arranged by size:
5208 943 676 443
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7270
XmaI C'CCGG_G
Cuts at: 1870 4595 1870
Size: 2725 4545
XmnI GAAnn'nnTTC
Cuts at: 5399 5399
Size: 7270
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2983 5078 276
Size: 53 83 186 936 1449 2095 2468
Fragments arranged by size:
2468 2095 1449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI
SfiI SrfI SwaI