Restriction map for
pFN21AE3308
(Circular) (Six-base) MAPSORT of: pFN21AE3308.seq Check: 2028 from: 1 to: 7114
pFN21AE3308 7114 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3905 3905
Size: 7114
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4924 278
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4457 1937
Size: 2520 4594
Acc65I G'GTAC_C
Cuts at: 4435 4435
Size: 7114
AclI AA'CG_TT
Cuts at: 1563 5241 5614 1563
Size: 3678 373 3063
Fragments arranged by size:
3678 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2042 2166 2429 2771 3135 3451 5177 6561
Size: 410 124 263 342 364 316 1726 1384
Cuts at: 6561 6829 1632
Size: 268 1917
Fragments arranged by size:
1917 1726 1384 410 364 342 316 268
263 124
AfeI AGC'GCT
Cuts at: 2858 2858
Size: 7114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6917
AflIII A'CryG_T
Cuts at: 1203 2384 2739 6013 6990 1203
Size: 1181 355 3274 977 1327
Fragments arranged by size:
3274 1327 1181 977 355
AgeI A'CCGG_T
Cuts at: 4364 5945 4364
Size: 1581 5533
AhdI GACnn_n'nnGTC
Cuts at: 5843 5843
Size: 7114
AleI CACnn'nnGTG
Cuts at: 1166 3296 1166
Size: 2130 4984
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4277 1184
Size: 3093 4021
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3147 3179 3147
Size: 32 7082
AlwNI CAG_nnn'CTG
Cuts at: 3004 3167 4144 4857 6429 3004
Size: 163 977 713 1572 3689
Fragments arranged by size:
3689 1572 977 713 163
ApaI G_GGCC'C
Cuts at: 2029 4352 2029
Size: 2323 4791
ApaLI G'TGCA_C
Cuts at: 5171 6327 5171
Size: 1156 5958
ApoI r'AATT_y
Cuts at: 1493 2045 2108 3363 3374 3639 3827 3851 4423
Size: 552 63 1255 11 265 188 24 572
Cuts at: 4423 4649 4801 6910 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1255 572 552 265 226 188
152 63 24 11
AseI AT'TA_AT
Cuts at: 160 1032 5668 160
Size: 872 4636 1606
Fragments arranged by size:
4636 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
AvaI C'yCGr_G
Cuts at: 1870 1943 2868 4439 1870
Size: 73 925 1571 4545
Fragments arranged by size:
4545 1571 925 73
BamHI G'GATC_C
Cuts at: 4010 4444 4811 4010
Size: 434 367 6313
Fragments arranged by size:
6313 434 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2345 4435 5890 618
Size: 325 217 627 558 2090 1455 1842
Fragments arranged by size:
2090 1842 1455 627 558 325 217
BanII G_rGCy'C
Cuts at: 1391 2029 4352 4433 1391
Size: 638 2323 81 4072
Fragments arranged by size:
4072 2323 638 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2213 2455 928
Size: 971 314 242 5587
Fragments arranged by size:
5587 971 314 242
BbvCI CC'TCA_GC
Cuts at: 2033 2033
Size: 7114
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5305 5339 1903
Size: 34 3368 34 3678
Fragments arranged by size:
3678 3368 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5006 6216 5006
Size: 1210 5904
BclI T'GATC_A
Cuts at: 1548 2629 1548
Size: 1081 6033
BfrBI ATG'CAT
Cuts at: 2551 4003 2551
Size: 1452 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5725 136
Size: 107 122 71 631 605 4053 1525
Fragments arranged by size:
4053 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4126 7109 4126
Size: 2983 4131
BlpI GC'TnA_GC
Cuts at: 4531 4531
Size: 7114
Bme1580I G_kGCm'C
Cuts at: 1790 2029 4352 5175 6331 1790
Size: 239 2323 823 1156 2573
Fragments arranged by size:
2573 2323 1156 823 239
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7114
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2947 3725 4845
Size: 416 53 83 399 24 1530 778 1120
Cuts at: 4845 5803 442
Size: 958 1753
Fragments arranged by size:
1753 1530 1120 958 778 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7114
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4275 4275
Size: 7114
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2672 3534 4166 5774 1239
Size: 1433 862 632 1608 2579
Fragments arranged by size:
2579 1608 1433 862 632
Bpu10I CC'TnA_GC
Cuts at: 2033 2033
Size: 7114
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2486 2588 5239 6104 6402 2486
Size: 102 2651 865 298 3198
Fragments arranged by size:
3198 2651 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3720 5777 882
Size: 617 2221 2057 2219
Fragments arranged by size:
2221 2219 2057 617
BsaAI yAC'GTr
Cuts at: 493 1204 2385 2740 4905 493
Size: 711 1181 355 2165 2702
Fragments arranged by size:
2702 2165 1181 711 355
BsaBI GATnn'nnATC
Cuts at: 7108 7108
Size: 7114
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4921 5303 5938 275
Size: 53 83 186 936 3388 382 635 1451
Fragments arranged by size:
3388 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4364 5547 5945 6219 6366 6777 4364
Size: 1183 398 274 147 411 4701
Fragments arranged by size:
4701 1183 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2356 2386 3147 3177 3717
Size: 30 165 30 953 30 761 30 540
Cuts at: 3717 3747 1178
Size: 30 4545
Fragments arranged by size:
4545 953 761 540 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3624 1186
Size: 2438 4676
BseYI C'CCAG_C
Cuts at: 3210 3504 6317 6813 3210
Size: 294 2813 496 3511
Fragments arranged by size:
3511 2813 496 294
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2229 1163
Size: 573 493 6048
Fragments arranged by size:
6048 573 493
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4564 5325 6353 1540
Size: 275 186 2563 761 1028 2301
Fragments arranged by size:
2563 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2700 2875 4232 5175 5260 6331 2700
Size: 175 1357 943 85 1071 3483
Fragments arranged by size:
3483 1357 1071 943 175 85
BsmI GAATG_Cn'
Cuts at: 4625 4718 4625
Size: 93 7021
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2029 2700 2875 4232 4352 4433 5175
Size: 399 239 671 175 1357 120 81 742
Cuts at: 5175 5260 6331 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1357 1071 742 671 399 239 175
120 85 81
BspEI T'CCGG_A
Cuts at: 6777 6777
Size: 7114
BspHI T'CATG_A
Cuts at: 2202 2632 3804 5003 6733 2202
Size: 430 1172 1199 1730 2583
Fragments arranged by size:
2583 1730 1199 1172 430
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2776 3277 3905 4469 844
Size: 1932 501 628 564 3489
Fragments arranged by size:
3489 1932 628 564 501
BsrBI CCG'CTC
Cuts at: 1394 1823 5001 1394
Size: 429 3178 3507
Fragments arranged by size:
3507 3178 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3586 3783 5609 5783 65
Size: 1183 2338 197 1826 174 1396
Fragments arranged by size:
2338 1826 1396 1183 197 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4212 4364 5758 5945 1700
Size: 110 2402 152 1394 187 2869
Fragments arranged by size:
2869 2402 1394 187 152 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7114
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7114
BssSI C'ACGA_G
Cuts at: 5174 6186 5174
Size: 1012 6102
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2784 794
Size: 1990 5124
BstBI TT'CG_AA
Cuts at: 5918 5918
Size: 7114
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7114
BstXI CCAn_nnnn'nTGG
Cuts at: 2120 3772 4177 2120
Size: 1652 405 5057
Fragments arranged by size:
5057 1652 405
BstYI r'GATC_y
Cuts at: 1972 4010 4126 4444 4811 5196 5213 6665 6751
Size: 2038 116 318 367 385 17 1452 86
Cuts at: 6751 6763 7109 1972
Size: 12 346 1977
Fragments arranged by size:
2038 1977 1452 385 367 346 318 116
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2454 3168 2454
Size: 714 6400
BtgI C'CryG_G
Cuts at: 513 1065 2113 3498 4170 4286 513
Size: 552 1048 1385 672 116 3341
Fragments arranged by size:
3341 1385 1048 672 552 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3616 487
Size: 683 56 2390 3985
Fragments arranged by size:
3985 2390 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 3038 4630 5424 5444 2241
Size: 797 1592 794 20 3911
Fragments arranged by size:
3911 1592 797 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3325 3360 529
Size: 35 2761 35 4283
Fragments arranged by size:
4283 2761 35 35
DraI TTT'AAA
Cuts at: 4418 4418
Size: 7114
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4255 6121 809
Size: 507 2939 1866 1802
Fragments arranged by size:
2939 1866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4210 4561 5450
Size: 54 1425 50 275 18 2380 351 889
Cuts at: 5450 7079 8
Size: 1629 43
Fragments arranged by size:
2380 1629 1425 889 351 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4561 1537
Size: 275 2749 4090
Fragments arranged by size:
4090 2749 275
EarI CTCTTCn'nnn_
Cuts at: 1353 5044 1353
Size: 3691 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5701 6075 6221 387
Size: 1064 138 4112 374 146 1280
Fragments arranged by size:
4112 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2166 2429 2672 2771 3135 3451
Size: 393 410 124 263 243 99 364 316
Cuts at: 3451 3534 4166 5177 5774 6561 6829 1239
Size: 83 632 1011 597 787 268 1524
Fragments arranged by size:
1524 1011 787 632 597 410 393 364
316 268 263 243 124 99 83
EcoO109I rG'GnC_Cy
Cuts at: 2818 3001 2818
Size: 183 6931
EcoRI G'AATT_C
Cuts at: 2108 4423 4801 2108
Size: 2315 378 4421
Fragments arranged by size:
4421 2315 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2180 2243 2180
Size: 63 7051
FspI TGC'GCA
Cuts at: 5620 5620
Size: 7114
HaeII r_GCGC'y
Cuts at: 1678 2860 6261 1678
Size: 1182 3401 2531
Fragments arranged by size:
3401 2531 1182
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2356 2388 3550 3582 3786
Size: 32 135 32 784 32 1162 32 204
Cuts at: 3786 3818 5755 5787 5829 5861 1373
Size: 32 1937 32 42 32 2626
Fragments arranged by size:
2626 1937 1162 784 204 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4458 4704 669
Size: 988 281 2520 246 3079
Fragments arranged by size:
3079 2520 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2462 748
Size: 1714 5400
HpaI GTT'AAC
Cuts at: 4704 4704
Size: 7114
KpnI G_GTAC'C
Cuts at: 4439 4439
Size: 7114
MfeI C'AATT_G
Cuts at: 4713 4713
Size: 7114
MluI A'CGCG_T
Cuts at: 6990 6990
Size: 7114
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3657 6228 6412 1305
Size: 2352 2571 184 2007
Fragments arranged by size:
2571 2352 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5292
Fragments arranged by size:
5292 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2118 3296 3770 4175 5072 5431 5590
Size: 648 952 1178 474 405 897 359 159
Cuts at: 5590 518
Size: 2042
Fragments arranged by size:
2042 1178 952 897 648 474 405 359
159
MspA1I CmG'CkG
Cuts at: 1606 4141 4530 5207 6355 6600 1606
Size: 2535 389 677 1148 245 2120
Fragments arranged by size:
2535 2120 1148 677 389 245
NaeI GCC'GGC
Cuts at: 4214 4214
Size: 7114
NcoI C'CATG_G
Cuts at: 513 1065 2113 4170 4286 513
Size: 552 1048 2057 116 3341
Fragments arranged by size:
3341 2057 1048 552 116
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7114
NgoMIV G'CCGG_C
Cuts at: 4212 4212
Size: 7114
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7114
NotI GC'GGCC_GC
Cuts at: 4561 4561
Size: 7114
NsiI A_TGCA'T
Cuts at: 2553 4005 2553
Size: 1452 5662
NspI r_CATG'y
Cuts at: 1132 1229 2555 2797 4003 4472 6017 1132
Size: 97 1326 242 1206 469 1545 2229
Fragments arranged by size:
2229 1545 1326 1206 469 242 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7114
PciI A'CATG_T
Cuts at: 6013 6013
Size: 7114
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4020 4144 1108
Size: 2912 124 4078
Fragments arranged by size:
4078 2912 124
PfoI T'CCnGG_A
Cuts at: 2214 2508 2214
Size: 294 6820
PmeI GTTT'AAAC
Cuts at: 4418 4418
Size: 7114
PmlI CAC'GTG
Cuts at: 2740 2740
Size: 7114
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2632 2664 3147 3179 5183 5215 6696 6728 2632
Size: 32 483 32 2004 32 1481 32 3018
Fragments arranged by size:
3018 2004 1481 483 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 4088 1613
Size: 2475 4639
PsiI TTA'TAA
Cuts at: 4684 4684
Size: 7114
PspOMI G'GGCC_C
Cuts at: 2025 4348 2025
Size: 2323 4791
PspXI vC'TCGA_Gb
Cuts at: 2868 2868
Size: 7114
PstI C_TGCA'G
Cuts at: 830 3288 4466 830
Size: 2458 1178 3478
Fragments arranged by size:
3478 2458 1178
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7114
PvuII CAG'CTG
Cuts at: 4141 4141
Size: 7114
SalI G'TCGA_C
Cuts at: 1936 4456 1936
Size: 2520 4594
SbfI CC_TGCA'GG
Cuts at: 3288 4466 3288
Size: 1178 5936
ScaI AGT'ACT
Cuts at: 5362 7074 5362
Size: 1712 5402
SfcI C'TryA_G
Cuts at: 826 1045 3267 3284 3918 4462 5597 6278 6469
Size: 219 2222 17 634 544 1135 681 191
Cuts at: 6469 826
Size: 1471
Fragments arranged by size:
2222 1471 1135 681 634 544 219 191
17
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7114
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7114
SmaI CCC'GGG
Cuts at: 1872 4441 1872
Size: 2569 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2465 2567 2868 5218 6119 6381
Size: 197 926 522 102 301 2350 901 262
Cuts at: 6381 820
Size: 1553
Fragments arranged by size:
2350 1553 926 901 522 301 262 197
102
SnaBI TAC'GTA
Cuts at: 493 4905 493
Size: 4412 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7114
SphI G_CATG'C
Cuts at: 1132 4003 4472 1132
Size: 2871 469 3774
Fragments arranged by size:
3774 2871 469
SspI AAT'ATT
Cuts at: 5 52 5038 5
Size: 47 4986 2081
Fragments arranged by size:
4986 2081 47
StuI AGG'CCT
Cuts at: 1517 3282 4197 1517
Size: 1765 915 4434
Fragments arranged by size:
4434 1765 915
StyI C'CwwG_G
Cuts at: 513 1065 2113 4170 4286 4553 513
Size: 552 1048 2057 116 267 3074
Fragments arranged by size:
3074 2057 1048 552 267 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3127 3327 3834 5141 5158 5311 5496
Size: 1255 1547 200 507 1307 17 153 185
Cuts at: 5496 5941 325
Size: 445 1498
Fragments arranged by size:
1547 1498 1307 1255 507 445 200 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2841 3940 5360
Size: 274 80 33 51 1443 864 1099 1420
Cuts at: 5360 7072 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1420 1099 864 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2724 2756 3717 3749 3780 3812 7074 7106 2724
Size: 32 961 32 31 32 3262 32 2732
Fragments arranged by size:
3262 2732 961 32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 1316 2417 1316
Size: 1101 6013
XbaI T'CTAG_A
Cuts at: 4450 4450
Size: 7114
XhoI C'TCGA_G
Cuts at: 1943 2868 1943
Size: 925 6189
XmaI C'CCGG_G
Cuts at: 1870 4439 1870
Size: 2569 4545
XmnI GAAnn'nnTTC
Cuts at: 2210 2504 2661 5243 2210
Size: 294 157 2582 4081
Fragments arranged by size:
4081 2582 294 157
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4922 276
Size: 53 83 186 936 3388 2468
Fragments arranged by size:
3388 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI
PacI PpuMI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI