Restriction map for
pFN21AE3307
(Circular) (Six-base) MAPSORT of: pFN21AE3307.seq Check: 8071 from: 1 to: 7099
pFN21AE3307 7099 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4909 278
Size: 53 83 186 936 3373 2468
Fragments arranged by size:
3373 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4442 1937
Size: 2505 4594
Acc65I G'GTAC_C
Cuts at: 4420 4420
Size: 7099
AclI AA'CG_TT
Cuts at: 1563 5226 5599 1563
Size: 3663 373 3063
Fragments arranged by size:
3663 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3151 4339 5162 6546 6814 1632
Size: 1519 1188 823 1384 268 1917
Fragments arranged by size:
1917 1519 1384 1188 823 268
AfeI AGC'GCT
Cuts at: 2303 2303
Size: 7099
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6902
AflIII A'CryG_T
Cuts at: 1203 5998 6975 1203
Size: 4795 977 1327
Fragments arranged by size:
4795 1327 977
AgeI A'CCGG_T
Cuts at: 4139 5930 4139
Size: 1791 5308
AhdI GACnn_n'nnGTC
Cuts at: 5828 5828
Size: 7099
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7099
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7099
AlwNI CAG_nnn'CTG
Cuts at: 3118 4842 6414 3118
Size: 1724 1572 3803
Fragments arranged by size:
3803 1724 1572
ApaLI G'TGCA_C
Cuts at: 3689 5156 6312 3689
Size: 1467 1156 4476
Fragments arranged by size:
4476 1467 1156
ApoI r'AATT_y
Cuts at: 1493 3815 4408 4634 4786 6895 1493
Size: 2322 593 226 152 2109 1697
Fragments arranged by size:
2322 2109 1697 593 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5653 160
Size: 872 4621 1606
Fragments arranged by size:
4621 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2534 2608 4424 1870
Size: 73 77 514 74 1816 4545
Fragments arranged by size:
4545 1816 514 77 74 73
BamHI G'GATC_C
Cuts at: 3245 3572 4429 4796 3245
Size: 327 857 367 5548
Fragments arranged by size:
5548 857 367 327
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2573 4420 5875 618
Size: 325 217 627 786 1847 1455 1842
Fragments arranged by size:
1847 1842 1455 786 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2320 2365 4418 1391
Size: 929 45 2053 4072
Fragments arranged by size:
4072 2053 929 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3855 928
Size: 971 1956 4172
Fragments arranged by size:
4172 1956 971
BbvCI CC'TCA_GC
Cuts at: 2441 2453 2599 2441
Size: 12 146 6941
Fragments arranged by size:
6941 146 12
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5290 5324 1903
Size: 34 3353 34 3678
Fragments arranged by size:
3678 3353 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4991 6201 4991
Size: 1210 5889
BclI T'GATC_A
Cuts at: 1548 3514 1548
Size: 1966 5133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5710 136
Size: 107 122 71 631 605 337 3701 1525
Fragments arranged by size:
3701 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 7094 7094
Size: 7099
BlpI GC'TnA_GC
Cuts at: 3335 4516 3335
Size: 1181 5918
Bme1580I G_kGCm'C
Cuts at: 1790 2268 3693 5160 6316 1790
Size: 478 1425 1467 1156 2573
Fragments arranged by size:
2573 1467 1425 1156 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7099
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3967 4830 5788
Size: 416 53 83 399 24 2550 863 958
Cuts at: 5788 442
Size: 1753
Fragments arranged by size:
2550 1753 958 863 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7099
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2669
Size: 7099
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3017 5759 1239
Size: 1778 2742 2579
Fragments arranged by size:
2742 2579 1778
Bpu10I CC'TnA_GC
Cuts at: 2441 2453 2599 2441
Size: 12 146 6941
Fragments arranged by size:
6941 146 12
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5224 6089 6387 5224
Size: 865 298 5936
Fragments arranged by size:
5936 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5762 882
Size: 617 4263 2219
Fragments arranged by size:
4263 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4890 493
Size: 711 3686 2702
Fragments arranged by size:
3686 2702 711
BsaBI GATnn'nnATC
Cuts at: 3614 7093 3614
Size: 3479 3620
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2347 4906 5288
Size: 53 83 186 936 624 190 2559 382
Cuts at: 5288 5923 275
Size: 635 1451
Fragments arranged by size:
2559 1451 936 635 624 382 190 186
83 53
BsaWI w'CCGG_w
Cuts at: 2121 2931 3641 4139 5532 5930 6204 6351 6762
Size: 810 710 498 1393 398 274 147 411
Cuts at: 6762 2121
Size: 2458
Fragments arranged by size:
2458 1393 810 710 498 411 398 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6874
Fragments arranged by size:
6874 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2697 2893 3044 3307 3310 3713 1186
Size: 1511 196 151 263 3 403 4572
Fragments arranged by size:
4572 1511 403 263 196 151 3
BseYI C'CCAG_C
Cuts at: 2279 2420 2488 3088 4034 4200 6302 6798 2279
Size: 141 68 600 946 166 2102 496 2580
Fragments arranged by size:
2580 2102 946 600 496 166 141 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2401 1163
Size: 573 665 5861
Fragments arranged by size:
5861 665 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2084 2260 2437 2571 4549 5310
Size: 275 186 83 176 177 134 1978 761
Cuts at: 5310 6338 1540
Size: 1028 2301
Fragments arranged by size:
2301 1978 1028 761 275 186 177 176
134 83
BsiHKAI G_wGCw'C
Cuts at: 2320 2365 3301 3693 3945 3972 4233 5160 5245
Size: 45 936 392 252 27 261 927 85
Cuts at: 5245 6316 2320
Size: 1071 3103
Fragments arranged by size:
3103 1071 936 927 392 261 252 85
45 27
BsmI GAATG_Cn'
Cuts at: 3139 3407 3885 4610 4703 3139
Size: 268 478 725 93 5535
Fragments arranged by size:
5535 725 478 268 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2268 2320 2365 3301 3693 3945 3972
Size: 399 478 52 45 936 392 252 27
Cuts at: 3972 4233 4418 5160 5245 6316 1391
Size: 261 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 936 742 478 399 392 261
252 185 85 52 45 27
BspEI T'CCGG_A
Cuts at: 2931 6762 2931
Size: 3831 3268
BspHI T'CATG_A
Cuts at: 3279 4176 4988 6718 3279
Size: 897 812 1730 3660
Fragments arranged by size:
3660 1730 897 812
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4454 844
Size: 3610 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2401 2613 4986 1394
Size: 429 578 212 2373 3507
Fragments arranged by size:
3507 2373 578 429 212
BsrDI GCAATG_nn'
Cuts at: 65 1248 3433 3540 5594 5768 65
Size: 1183 2185 107 2054 174 1396
Fragments arranged by size:
2185 2054 1396 1183 174 107
BsrFI r'CCGG_y
Cuts at: 1700 1810 2034 2274 3270 3771 4139 5743 5930
Size: 110 224 240 996 501 368 1604 187
Cuts at: 5930 1700
Size: 2869
Fragments arranged by size:
2869 1604 996 501 368 240 224 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7099
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7099
BssSI C'ACGA_G
Cuts at: 5159 6171 5159
Size: 1012 6087
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3380 794
Size: 2586 4513
BstBI TT'CG_AA
Cuts at: 5903 5903
Size: 7099
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7099
BstXI CCAn_nnnn'nTGG
Cuts at: 2304 2304
Size: 7099
BstYI r'GATC_y
Cuts at: 1972 3245 3572 3704 4429 4796 5181 5198 6650
Size: 1273 327 132 725 367 385 17 1452
Cuts at: 6650 6736 6748 7094 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1273 725 385 367 346 327
132 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2726 2766 2726
Size: 40 7059
BtgI C'CryG_G
Cuts at: 513 1065 2004 4119 513
Size: 552 939 2115 3493
Fragments arranged by size:
3493 2115 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6360
Fragments arranged by size:
6360 683 56
BtsI GCAGTG_nn'
Cuts at: 2108 3225 3921 3929 4615 5409 5429 2108
Size: 1117 696 8 686 794 20 3778
Fragments arranged by size:
3778 1117 794 696 686 20 8
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7099
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7064
DraI TTT'AAA
Cuts at: 3073 3787 4403 3073
Size: 714 616 5769
Fragments arranged by size:
5769 714 616
DraIII CAC_nnn'GTG
Cuts at: 2393 2393
Size: 7099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2850 3199 6106 809
Size: 507 1534 349 2907 1802
Fragments arranged by size:
2907 1802 1534 507 349
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2081 2257 2434
Size: 54 1425 50 275 18 251 176 177
Cuts at: 2434 2464 4546 5435 7064 8
Size: 30 2082 889 1629 43
Fragments arranged by size:
2082 1629 1425 889 275 251 177 176
54 50 43 30 18
EagI C'GGCC_G
Cuts at: 1537 1812 2081 2257 2434 4546 1537
Size: 275 269 176 177 2112 4090
Fragments arranged by size:
4090 2112 275 269 177 176
EarI CTCTTCn'nnn_
Cuts at: 1353 3014 3806 4013 5029 1353
Size: 1661 792 207 1016 3423
Fragments arranged by size:
3423 1661 1016 792 207
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 2400 2569 5686 6060 6206
Size: 1064 138 668 143 169 3117 374 146
Cuts at: 6206 387
Size: 1280
Fragments arranged by size:
3117 1280 1064 668 374 169 146 143
138
EcoICRI GAG'CTC
Cuts at: 2318 2363 2318
Size: 45 7054
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3017 3151 4339 5162 5759 6546 6814
Size: 393 1385 134 1188 823 597 787 268
Cuts at: 6814 1239
Size: 1524
Fragments arranged by size:
1524 1385 1188 823 787 597 393 268
134
EcoO109I rG'GnC_Cy
Cuts at: 2024 2538 3115 3416 4129 2024
Size: 514 577 301 713 4994
Fragments arranged by size:
4994 713 577 514 301
EcoRI G'AATT_C
Cuts at: 4408 4786 4408
Size: 378 6721
FspI TGC'GCA
Cuts at: 5605 5605
Size: 7099
HaeII r_GCGC'y
Cuts at: 1678 2305 6246 1678
Size: 627 3941 2531
Fragments arranged by size:
3941 2531 627
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 2926 2958 3013
Size: 32 135 32 1061 32 261 32 55
Cuts at: 3013 3045 5740 5772 5814 5846 1373
Size: 32 2695 32 42 32 2626
Fragments arranged by size:
2695 2626 1061 261 135 55 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4443 4689 669
Size: 988 281 2505 246 3079
Fragments arranged by size:
3079 2505 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7099
HpaI GTT'AAC
Cuts at: 4689 4689
Size: 7099
KpnI G_GTAC'C
Cuts at: 4424 4424
Size: 7099
MfeI C'AATT_G
Cuts at: 3507 4698 3507
Size: 1191 5908
MluI A'CGCG_T
Cuts at: 6975 6975
Size: 7099
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2208 2428 6213 6397 1305
Size: 903 220 3785 184 2007
Fragments arranged by size:
3785 2007 903 220 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5277
Fragments arranged by size:
5277 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 5057 5416 5575 518
Size: 648 1533 2358 359 159 2042
Fragments arranged by size:
2358 2042 1533 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2077 2145 4038 4200 4515 5192 6340 6585
Size: 471 68 1893 162 315 677 1148 245
Cuts at: 6585 1606
Size: 2120
Fragments arranged by size:
2120 1893 1148 677 471 315 245 162
68
NaeI GCC'GGC
Cuts at: 2036 2036
Size: 7099
NcoI C'CATG_G
Cuts at: 513 1065 2004 4119 513
Size: 552 939 2115 3493
Fragments arranged by size:
3493 2115 939 552
NdeI CA'TA_TG
Cuts at: 387 2050 387
Size: 1663 5436
NgoMIV G'CCGG_C
Cuts at: 2034 2034
Size: 7099
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7099
NotI GC'GGCC_GC
Cuts at: 4546 4546
Size: 7099
NspI r_CATG'y
Cuts at: 1132 1229 4457 6002 1132
Size: 97 3228 1545 2229
Fragments arranged by size:
3228 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2472 1348
Size: 1124 5975
PciI A'CATG_T
Cuts at: 5998 5998
Size: 7099
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3088 1108
Size: 1980 5119
PfoI T'CCnGG_A
Cuts at: 4326 4326
Size: 7099
PmeI GTTT'AAAC
Cuts at: 4403 4403
Size: 7099
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4343 4375 5168 5200 6681 6713 4343
Size: 32 793 32 1481 32 4729
Fragments arranged by size:
4729 1481 793 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2024 2538 3115 3416 4129 2024
Size: 514 577 301 713 4994
Fragments arranged by size:
4994 713 577 514 301
PshAI GACnn'nnGTC
Cuts at: 1613 2901 1613
Size: 1288 5811
PsiI TTA'TAA
Cuts at: 4669 4669
Size: 7099
PstI C_TGCA'G
Cuts at: 830 3215 4451 830
Size: 2385 1236 3478
Fragments arranged by size:
3478 2385 1236
PvuI CG_AT'CG
Cuts at: 2001 2571 2001
Size: 570 6529
PvuII CAG'CTG
Cuts at: 4038 4038
Size: 7099
SacI G_AGCT'C
Cuts at: 2320 2365 2320
Size: 45 7054
SalI G'TCGA_C
Cuts at: 1936 4441 1936
Size: 2505 4594
SanDI GG'GwC_CC
Cuts at: 2538 2538
Size: 7099
SapI GCTCTTCn'nnn_
Cuts at: 3014 3806 4013 3014
Size: 792 207 6100
Fragments arranged by size:
6100 792 207
SbfI CC_TGCA'GG
Cuts at: 4451 4451
Size: 7099
ScaI AGT'ACT
Cuts at: 5347 7059 5347
Size: 1712 5387
SfcI C'TryA_G
Cuts at: 826 1045 3211 4168 4447 5582 6263 6454 826
Size: 219 2166 957 279 1135 681 191 1471
Fragments arranged by size:
2166 1471 1135 957 681 279 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7099
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7099
SmaI CCC'GGG
Cuts at: 1872 2022 2536 4426 1872
Size: 150 514 1890 4545
Fragments arranged by size:
4545 1890 514 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5203 6104 6366 820
Size: 197 926 3260 901 262 1553
Fragments arranged by size:
3260 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4890 493
Size: 4397 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7099
SphI G_CATG'C
Cuts at: 1132 4457 1132
Size: 3325 3774
SspI AAT'ATT
Cuts at: 5 52 5023 5
Size: 47 4971 2081
Fragments arranged by size:
4971 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7099
StyI C'CwwG_G
Cuts at: 513 1065 2004 2268 2549 2688 2961 2978 3867
Size: 552 939 264 281 139 273 17 889
Cuts at: 3867 4119 4538 513
Size: 252 419 3074
Fragments arranged by size:
3074 939 889 552 419 281 273 264
252 139 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2658 3125 5126 5143 5296 5481 5926
Size: 1255 1078 467 2001 17 153 185 445
Cuts at: 5926 325
Size: 1498
Fragments arranged by size:
2001 1498 1255 1078 467 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5345 7057 96
Size: 274 80 33 51 1443 3368 1712 138
Fragments arranged by size:
3368 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7059 7091 7059
Size: 32 7067
Tth111I GACn'n_nGTC
Cuts at: 1316 2558 1316
Size: 1242 5857
XbaI T'CTAG_A
Cuts at: 4435 4435
Size: 7099
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7099
XmaI C'CCGG_G
Cuts at: 1870 2020 2534 4424 1870
Size: 150 514 1890 4545
Fragments arranged by size:
4545 1890 514 150
XmnI GAAnn'nnTTC
Cuts at: 3097 3498 5228 3097
Size: 401 1730 4968
Fragments arranged by size:
4968 1730 401
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4907 276
Size: 53 83 186 936 3373 2468
Fragments arranged by size:
3373 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PmlI PspOMI PspXI
PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI
XcmI