Restriction map for
pFN21AE3306
(Circular) (Six-base) MAPSORT of: pFN21AE3306.seq Check: 2117 from: 1 to: 7015
pFN21AE3306 7015 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3594 3594
Size: 7015
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3626 4825 278
Size: 53 83 186 936 2090 1199 2468
Fragments arranged by size:
2468 2090 1199 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3285 4358 1937
Size: 1348 1073 4594
Fragments arranged by size:
4594 1348 1073
Acc65I G'GTAC_C
Cuts at: 3735 4336 3735
Size: 601 6414
AclI AA'CG_TT
Cuts at: 1563 5142 5515 1563
Size: 3579 373 3063
Fragments arranged by size:
3579 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2039 3356 3722 3940 4259 4285 5078 6462
Size: 407 1317 366 218 319 26 793 1384
Cuts at: 6462 6730 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1317 793 407 366 319 268
218 26
AfeI AGC'GCT
Cuts at: 3989 4069 3989
Size: 80 6935
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6818
AflIII A'CryG_T
Cuts at: 1203 3789 5914 6891 1203
Size: 2586 2125 977 1327
Fragments arranged by size:
2586 2125 1327 977
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 2755 3793 5744 2755
Size: 1038 1951 4026
Fragments arranged by size:
4026 1951 1038
AleI CACnn'nnGTG
Cuts at: 1166 3782 1166
Size: 2616 4399
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7015
AlwNI CAG_nnn'CTG
Cuts at: 2658 2837 3193 3250 3568 4758 6330 2658
Size: 179 356 57 318 1190 1572 3343
Fragments arranged by size:
3343 1572 1190 356 318 179 57
ApaI G_GGCC'C
Cuts at: 4295 4295
Size: 7015
ApaLI G'TGCA_C
Cuts at: 5072 6228 5072
Size: 1156 5859
ApoI r'AATT_y
Cuts at: 1493 2049 2113 4324 4550 4702 6811 1493
Size: 556 64 2211 226 152 2109 1697
Fragments arranged by size:
2211 2109 1697 556 226 152 64
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 3845 4052 4186 4340 1870
Size: 73 1902 207 134 154 4545
Fragments arranged by size:
4545 1902 207 154 134 73
BamHI G'GATC_C
Cuts at: 3600 3692 4345 4712 3600
Size: 92 653 367 5903
Fragments arranged by size:
5903 653 367 92
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3269 3735 3761 4178 4336
Size: 325 217 627 1482 466 26 417 158
Cuts at: 4336 5791 618
Size: 1455 1842
Fragments arranged by size:
1842 1482 1455 627 466 417 325 217
158 26
BanII G_rGCy'C
Cuts at: 1391 3183 3933 4000 4295 4334 1391
Size: 1792 750 67 295 39 4072
Fragments arranged by size:
4072 1792 750 295 67 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2220 2232 2798 3554 928
Size: 971 321 12 566 756 4389
Fragments arranged by size:
4389 971 756 566 321 12
BbvCI CC'TCA_GC
Cuts at: 3377 4071 3377
Size: 694 6321
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2261 2295 2348 2382 3143 3177 3973
Size: 34 324 34 53 34 761 34 796
Cuts at: 3973 4007 5206 5240 1903
Size: 34 1199 34 3678
Fragments arranged by size:
3678 1199 796 761 324 53 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4907 6117 4907
Size: 1210 5805
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3648 5626 136
Size: 107 122 71 631 605 1976 1978 1525
Fragments arranged by size:
1978 1976 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3462 7010 3462
Size: 3548 3467
BlpI GC'TnA_GC
Cuts at: 3638 4432 3638
Size: 794 6221
Bme1580I G_kGCm'C
Cuts at: 1790 3153 3272 4181 4295 5076 6232 1790
Size: 1363 119 909 114 781 1156 2573
Fragments arranged by size:
2573 1363 1156 909 781 119 114
BmgBI CAC'GTC
Cuts at: 1318 3372 1318
Size: 2054 4961
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4746 5704 442
Size: 416 53 83 399 24 3329 958 1753
Fragments arranged by size:
3329 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2680 5675 1239
Size: 1441 2995 2579
Fragments arranged by size:
2995 2579 1441
Bpu10I CC'TnA_GC
Cuts at: 2830 3377 4071 2830
Size: 547 694 5774
Fragments arranged by size:
5774 694 547
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2168 3167 3383 3482 3602 3641 4067 4292 5140
Size: 999 216 99 120 39 426 225 848
Cuts at: 5140 6005 6303 2168
Size: 865 298 2880
Fragments arranged by size:
2880 999 865 848 426 298 225 216
120 99 39
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2930 3506 4097 5678 882
Size: 617 1431 576 591 1581 2219
Fragments arranged by size:
2219 1581 1431 617 591 576
BsaAI yAC'GTr
Cuts at: 493 1204 4140 4806 493
Size: 711 2936 666 2702
Fragments arranged by size:
2936 2702 711 666
BsaBI GATnn'nnATC
Cuts at: 3467 7009 3467
Size: 3542 3473
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3623 4822 5204 5839
Size: 53 83 186 936 2090 1199 382 635
Cuts at: 5839 275
Size: 1451
Fragments arranged by size:
2090 1451 1199 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5448 5846 6120 6267 6678 5448
Size: 398 274 147 411 5785
Fragments arranged by size:
5785 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2872 2902 3230 3260 1178
Size: 30 165 30 1469 30 328 30 4933
Fragments arranged by size:
4933 1469 328 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2165 2247 2579 2864 2867 2870 2879
Size: 913 66 82 332 285 3 3 9
Cuts at: 2879 2882 2885 2915 2918 2921 2924 2939 2942
Size: 3 3 30 3 3 3 15 3
Cuts at: 2942 2945 2948 2969 3251 3278 4072 1186
Size: 3 3 21 282 27 794 4129
Fragments arranged by size:
4129 913 794 332 285 282 82 66
30 27 21 15 9 3 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2268 3405 3934 4222 6218 6714 2268
Size: 1137 529 288 1996 496 2569
Fragments arranged by size:
2569 1996 1137 529 496 288
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2589 3572 3590 1163
Size: 573 853 983 18 4588
Fragments arranged by size:
4588 983 853 573 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2681 2713 2805 3064 3720 3783
Size: 275 186 680 32 92 259 656 63
Cuts at: 3783 4465 5226 6254 1540
Size: 682 761 1028 2301
Fragments arranged by size:
2301 1028 761 682 680 656 275 259
186 92 63 32
BsiHKAI G_wGCw'C
Cuts at: 2361 2885 3183 3492 3933 3966 5076 5161 6232
Size: 524 298 309 441 33 1110 85 1071
Cuts at: 6232 2361
Size: 3144
Fragments arranged by size:
3144 1110 1071 524 441 309 298 85
33
BsmI GAATG_Cn'
Cuts at: 2446 4526 4619 2446
Size: 2080 93 4842
Fragments arranged by size:
4842 2080 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2700 4238 2700
Size: 1538 5477
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2361 2885 3153 3183 3272 3492 3933
Size: 399 571 524 268 30 89 220 441
Cuts at: 3933 3966 4000 4181 4295 4334 5076 5161 6232
Size: 33 34 181 114 39 742 85 1071
Cuts at: 6232 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 571 524 441 399 268
220 181 114 89 85 39 34 33
30
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 4026 4904 6634 4026
Size: 878 1730 4407
Fragments arranged by size:
4407 1730 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2760 3213 3594 3639 4370 844
Size: 1916 453 381 45 731 3489
Fragments arranged by size:
3489 1916 731 453 381 45
BsrBI CCG'CTC
Cuts at: 1394 1823 2497 2554 2569 2581 2692 4902 1394
Size: 429 674 57 15 12 111 2210 3507
Fragments arranged by size:
3507 2210 674 429 111 57 15 12
BsrDI GCAATG_nn'
Cuts at: 65 1248 5510 5684 65
Size: 1183 4262 174 1396
Fragments arranged by size:
4262 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2939 3208 3647 5659 5846 1700
Size: 110 1129 269 439 2012 187 2869
Fragments arranged by size:
2869 2012 1129 439 269 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7015
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 5075 6087 5075
Size: 1012 6003
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3193 794
Size: 2399 4616
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 3724 1184
Size: 2540 4475
BstXI CCAn_nnnn'nTGG
Cuts at: 3784 3784
Size: 7015
BstYI r'GATC_y
Cuts at: 1972 3462 3600 3692 3886 4345 4712 5097 5114
Size: 1490 138 92 194 459 367 385 17
Cuts at: 5114 6566 6652 6664 7010 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1490 1452 459 385 367 346 194
138 92 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4197 4197
Size: 7015
BtgI C'CryG_G
Cuts at: 513 1065 2004 2684 3777 3783 4287 513
Size: 552 939 680 1093 6 504 3241
Fragments arranged by size:
3241 1093 939 680 552 504 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6276
Fragments arranged by size:
6276 683 56
BtsI GCAGTG_nn'
Cuts at: 4531 5325 5345 4531
Size: 794 20 6201
Fragments arranged by size:
6201 794 20
ClaI AT'CG_AT
Cuts at: 1554 3861 1554
Size: 2307 4708
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 4319 4319
Size: 7015
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6022 809
Size: 507 4706 1802
Fragments arranged by size:
4706 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2253 2678 2710
Size: 54 1425 50 275 18 423 425 32
Cuts at: 2710 2802 3780 4462 5351 6980 8
Size: 92 978 682 889 1629 43
Fragments arranged by size:
1629 1425 978 889 682 425 423 275
92 54 50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2678 2710 2802 3780 4462 1537
Size: 275 866 32 92 978 682 4090
Fragments arranged by size:
4090 978 866 682 275 92 32
EarI CTCTTCn'nnn_
Cuts at: 1353 2203 2417 2724 2841 2856 2910 2916 4945
Size: 850 214 307 117 15 54 6 2029
Cuts at: 4945 1353
Size: 3423
Fragments arranged by size:
3423 2029 850 307 214 117 54 15
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2576 5602 5976 6122 387
Size: 1064 138 987 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 987 374 146 138
EcoICRI GAG'CTC
Cuts at: 3181 3931 3181
Size: 750 6265
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2039 2680 3356 3722 3940 4259 4285
Size: 393 407 641 676 366 218 319 26
Cuts at: 4285 5078 5675 6462 6730 1239
Size: 793 597 787 268 1524
Fragments arranged by size:
1524 793 787 676 641 597 407 393
366 319 268 218 26
EcoNI CCTnn'n_nnAGG
Cuts at: 2150 3134 4200 2150
Size: 984 1066 4965
Fragments arranged by size:
4965 1066 984
EcoO109I rG'GnC_Cy
Cuts at: 2749 3894 4191 4278 4292 2749
Size: 1145 297 87 14 5472
Fragments arranged by size:
5472 1145 297 87 14
EcoRI G'AATT_C
Cuts at: 4324 4702 4324
Size: 378 6637
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3376 3970 3376
Size: 594 6421
FspI TGC'GCA
Cuts at: 5521 5521
Size: 7015
HaeII r_GCGC'y
Cuts at: 1678 3129 3991 4071 6162 1678
Size: 1451 862 80 2091 2531
Fragments arranged by size:
2531 2091 1451 862 80
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2741 2773 2798 2830 2945
Size: 32 135 32 1169 32 25 32 115
Cuts at: 2945 2977 3677 3709 3779 3811 5656 5688 5730
Size: 32 700 32 70 32 1845 32 42
Cuts at: 5730 5762 1373
Size: 32 2626
Fragments arranged by size:
2626 1845 1169 700 135 115 70 42
32 32 32 32 32 32 32 32
32 25
HincII GTy'rAC
Cuts at: 669 1657 1938 4359 4605 669
Size: 988 281 2421 246 3079
Fragments arranged by size:
3079 2421 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7015
HpaI GTT'AAC
Cuts at: 4605 4605
Size: 7015
KpnI G_GTAC'C
Cuts at: 3739 4340 3739
Size: 601 6414
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2348 2784 3651 6129 6313 1305
Size: 1043 436 867 2478 184 2007
Fragments arranged by size:
2478 2007 1043 867 436 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2255 10
Size: 54 1425 343 423 4770
Fragments arranged by size:
4770 1425 423 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3473 3782 4973 5332 5491 518
Size: 648 2307 309 1191 359 159 2042
Fragments arranged by size:
2307 2042 1191 648 359 309 159
MspA1I CmG'CkG
Cuts at: 1606 2533 2686 2819 3565 4222 4289 4431 5108
Size: 927 153 133 746 657 67 142 677
Cuts at: 5108 6256 6501 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 927 746 677 657 245 153
142 133 67
NaeI GCC'GGC
Cuts at: 3210 3649 3210
Size: 439 6576
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5524
Fragments arranged by size:
5524 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7015
NgoMIV G'CCGG_C
Cuts at: 3208 3647 3208
Size: 439 6576
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NspI r_CATG'y
Cuts at: 1132 1229 2339 3793 4373 5918 1132
Size: 97 1110 1454 580 1545 2229
Fragments arranged by size:
2229 1545 1454 1110 580 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7015
PciI A'CATG_T
Cuts at: 3789 5914 3789
Size: 2125 4890
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2658 3037 1108
Size: 1550 379 5086
Fragments arranged by size:
5086 1550 379
PfoI T'CCnGG_A
Cuts at: 2450 2450
Size: 7015
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PmlI CAC'GTG
Cuts at: 4140 4140
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3041 3073 5084 5116 6597 6629 3041
Size: 32 2011 32 1481 32 3427
Fragments arranged by size:
3427 2011 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2749 3894 2749
Size: 1145 5870
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7015
PsiI TTA'TAA
Cuts at: 4585 4585
Size: 7015
PspOMI G'GGCC_C
Cuts at: 4291 4291
Size: 7015
PstI C_TGCA'G
Cuts at: 830 3219 4367 830
Size: 2389 1148 3478
Fragments arranged by size:
3478 2389 1148
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 3565 4222 3565
Size: 657 6358
SacI G_AGCT'C
Cuts at: 3183 3933 3183
Size: 750 6265
SacII CC_GC'GG
Cuts at: 2687 4290 2687
Size: 1603 5412
SalI G'TCGA_C
Cuts at: 1936 4357 1936
Size: 2421 4594
SanDI GG'GwC_CC
Cuts at: 3894 3894
Size: 7015
SbfI CC_TGCA'GG
Cuts at: 3219 4367 3219
Size: 1148 5867
ScaI AGT'ACT
Cuts at: 5263 6975 5263
Size: 1712 5303
SfcI C'TryA_G
Cuts at: 826 1045 2632 3215 3286 3922 4213 4363 5498
Size: 219 1587 583 71 636 291 150 1135
Cuts at: 5498 6179 6370 826
Size: 681 191 1471
Fragments arranged by size:
1587 1471 1135 681 636 583 291 219
191 150 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 3847 4054 4342 1872
Size: 1975 207 288 4545
Fragments arranged by size:
4545 1975 288 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2147 3182 3398 3497 3617 3656
Size: 197 926 204 1035 216 99 120 39
Cuts at: 3656 4082 4307 5119 6020 6282 820
Size: 426 225 812 901 262 1553
Fragments arranged by size:
1553 1035 926 901 812 426 262 225
216 204 197 120 99 39
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 2339 4373 1132
Size: 1207 2034 3774
Fragments arranged by size:
3774 2034 1207
SspI AAT'ATT
Cuts at: 5 52 4939 5
Size: 47 4887 2081
Fragments arranged by size:
4887 2081 47
StuI AGG'CCT
Cuts at: 1517 3707 4206 1517
Size: 2190 499 4326
Fragments arranged by size:
4326 2190 499
StyI C'CwwG_G
Cuts at: 513 1065 2004 3765 4454 513
Size: 552 939 1761 689 3074
Fragments arranged by size:
3074 1761 939 689 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 3078 3092 3653 4214 5042 5059
Size: 1255 417 1081 14 561 561 828 17
Cuts at: 5059 5212 5397 5842 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1081 828 561 561 445 417
185 153 17 14
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5261 6973 96
Size: 274 80 33 51 1443 3284 1712 138
Fragments arranged by size:
3284 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2872 2904 3230 3262 3886 3918 6975 7007 2872
Size: 32 326 32 624 32 3057 32 2880
Fragments arranged by size:
3057 2880 624 326 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7015
XbaI T'CTAG_A
Cuts at: 4351 4351
Size: 7015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3569 3569
Size: 7015
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7015
XmaI C'CCGG_G
Cuts at: 1870 3845 4052 4340 1870
Size: 1975 207 288 4545
Fragments arranged by size:
4545 1975 288 207
XmnI GAAnn'nnTTC
Cuts at: 2052 2292 4034 5144 2052
Size: 240 1742 1110 3923
Fragments arranged by size:
3923 1742 1110 240
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3624 4823 276
Size: 53 83 186 936 2090 1199 2468
Fragments arranged by size:
2468 2090 1199 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SapI SexAI SfiI
SfoI SrfI SwaI