Restriction map for
pFN21AE3305
(Circular) (Six-base) MAPSORT of: pFN21AE3305.seq Check: 2124 from: 1 to: 7093
pFN21AE3305 7093 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4903 278
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2112 2575 3259 4436 1937
Size: 175 463 684 1177 4594
Fragments arranged by size:
4594 1177 684 463 175
Acc65I G'GTAC_C
Cuts at: 3985 4414 3985
Size: 429 6664
AclI AA'CG_TT
Cuts at: 1563 3093 5220 5593 1563
Size: 1530 2127 373 3063
Fragments arranged by size:
3063 2127 1530 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2189 4277 5156 6540 6808 1632
Size: 557 2088 879 1384 268 1917
Fragments arranged by size:
2088 1917 1384 879 557 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6896
AflIII A'CryG_T
Cuts at: 1203 3732 5992 6969 1203
Size: 2529 2260 977 1327
Fragments arranged by size:
2529 2260 1327 977
AgeI A'CCGG_T
Cuts at: 5924 5924
Size: 7093
AhdI GACnn_n'nnGTC
Cuts at: 2571 5822 2571
Size: 3251 3842
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7093
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7093
AlwNI CAG_nnn'CTG
Cuts at: 4836 6408 4836
Size: 1572 5521
ApaLI G'TGCA_C
Cuts at: 5150 6306 5150
Size: 1156 5937
ApoI r'AATT_y
Cuts at: 1493 2102 2426 2449 2580 2723 2761 3241 3304
Size: 609 324 23 131 143 38 480 63
Cuts at: 3304 3620 4402 4628 4780 6889 1493
Size: 316 782 226 152 2109 1697
Fragments arranged by size:
2109 1697 782 609 480 324 316 226
152 143 131 63 38 23
AseI AT'TA_AT
Cuts at: 160 1032 5647 160
Size: 872 4615 1606
Fragments arranged by size:
4615 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
AvaI C'yCGr_G
Cuts at: 1870 1943 4418 1870
Size: 73 2475 4545
Fragments arranged by size:
4545 2475 73
BamHI G'GATC_C
Cuts at: 4423 4790 4423
Size: 367 6726
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3779 3985 4137 4414 5869
Size: 325 217 627 1992 206 152 277 1455
Cuts at: 5869 618
Size: 1842
Fragments arranged by size:
1992 1842 1455 627 325 277 217 206
152
BanII G_rGCy'C
Cuts at: 1391 4320 4412 1391
Size: 2929 92 4072
Fragments arranged by size:
4072 2929 92
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3303 4013 928
Size: 971 1404 710 4008
Fragments arranged by size:
4008 1404 971 710
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3190 3224 5284 5318 1903
Size: 34 1253 34 2060 34 3678
Fragments arranged by size:
3678 2060 1253 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4985 6195 4985
Size: 1210 5883
BclI T'GATC_A
Cuts at: 1548 2058 3504 3511 1548
Size: 510 1446 7 5130
Fragments arranged by size:
5130 1446 510 7
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5704 136
Size: 107 122 71 631 605 4032 1525
Fragments arranged by size:
4032 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2383 7088 2383
Size: 4705 2388
BlpI GC'TnA_GC
Cuts at: 2199 4510 2199
Size: 2311 4782
Bme1580I G_kGCm'C
Cuts at: 1790 5154 6310 1790
Size: 3364 1156 2573
Fragments arranged by size:
3364 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7093
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4824 5782 442
Size: 416 53 83 399 24 3407 958 1753
Fragments arranged by size:
3407 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7093
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3908 5753 1239
Size: 2669 1845 2579
Fragments arranged by size:
2669 2579 1845
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5218 6083 6381 5218
Size: 865 298 5930
Fragments arranged by size:
5930 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2215 3197 5756 882
Size: 617 716 982 2559 2219
Fragments arranged by size:
2559 2219 982 716 617
BsaAI yAC'GTr
Cuts at: 493 1204 4884 493
Size: 711 3680 2702
Fragments arranged by size:
3680 2702 711
BsaBI GATnn'nnATC
Cuts at: 7087 7087
Size: 7093
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4900 5282 5917 275
Size: 53 83 186 936 3367 382 635 1451
Fragments arranged by size:
3367 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5526 5924 6198 6345 6756 5526
Size: 398 274 147 411 5863
Fragments arranged by size:
5863 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6868
Fragments arranged by size:
6868 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 7093
BseYI C'CCAG_C
Cuts at: 6296 6792 6296
Size: 496 6597
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3259 1163
Size: 573 1523 4997
Fragments arranged by size:
4997 1523 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4543 5304 6332 1540
Size: 275 186 2542 761 1028 2301
Fragments arranged by size:
2542 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5154 5239 6310 5154
Size: 85 1071 5937
Fragments arranged by size:
5937 1071 85
BsmI GAATG_Cn'
Cuts at: 2593 2877 4233 4604 4697 2593
Size: 284 1356 371 93 4989
Fragments arranged by size:
4989 1356 371 284 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4320 4412 5154 5239 6310 1391
Size: 399 2530 92 742 85 1071 2174
Fragments arranged by size:
2530 2174 1071 742 399 92 85
BspEI T'CCGG_A
Cuts at: 6756 6756
Size: 7093
BspHI T'CATG_A
Cuts at: 3195 4024 4177 4266 4982 6712 3195
Size: 829 153 89 716 1730 3576
Fragments arranged by size:
3576 1730 829 716 153 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4448 844
Size: 3604 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4980 1394
Size: 429 3157 3507
Fragments arranged by size:
3507 3157 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5588 5762 65
Size: 1183 4340 174 1396
Fragments arranged by size:
4340 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5737 5924 1700
Size: 110 3927 187 2869
Fragments arranged by size:
3927 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2976 96
Size: 2880 4213
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7093
BssSI C'ACGA_G
Cuts at: 5153 6165 5153
Size: 1012 6081
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7093
BstBI TT'CG_AA
Cuts at: 5897 5897
Size: 7093
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7093
BstXI CCAn_nnnn'nTGG
Cuts at: 2836 3790 2836
Size: 954 6139
BstYI r'GATC_y
Cuts at: 1972 2383 3832 4184 4423 4790 5175 5192 6644
Size: 411 1449 352 239 367 385 17 1452
Cuts at: 6644 6730 6742 7088 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1449 411 385 367 352 346
239 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6541
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2179 487
Size: 683 56 953 5401
Fragments arranged by size:
5401 953 683 56
BtsI GCAGTG_nn'
Cuts at: 4295 4609 5403 5423 4295
Size: 314 794 20 5965
Fragments arranged by size:
5965 794 314 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7093
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7058
DraI TTT'AAA
Cuts at: 4397 4397
Size: 7093
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6100 809
Size: 507 4784 1802
Fragments arranged by size:
4784 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4540 5429 7058
Size: 54 1425 50 275 18 2710 889 1629
Cuts at: 7058 8
Size: 43
Fragments arranged by size:
2710 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4540 1537
Size: 275 2728 4090
Fragments arranged by size:
4090 2728 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4079 5023 1353
Size: 2726 944 3423
Fragments arranged by size:
3423 2726 944
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5680 6054 6200 387
Size: 1064 138 4091 374 146 1280
Fragments arranged by size:
4091 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2189 3908 4277 5156 5753 6540 6808
Size: 393 557 1719 369 879 597 787 268
Cuts at: 6808 1239
Size: 1524
Fragments arranged by size:
1719 1524 879 787 597 557 393 369
268
EcoO109I rG'GnC_Cy
Cuts at: 2407 2407
Size: 7093
EcoRI G'AATT_C
Cuts at: 3241 4402 4780 3241
Size: 1161 378 5554
Fragments arranged by size:
5554 1161 378
EcoRV GAT'ATC
Cuts at: 2349 2349
Size: 7093
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4117 4117
Size: 7093
FspI TGC'GCA
Cuts at: 5599 5599
Size: 7093
HaeII r_GCGC'y
Cuts at: 1678 6240 1678
Size: 4562 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2557 2589 3496 3528 3609
Size: 32 135 32 985 32 907 32 81
Cuts at: 3609 3629 3641 3661 3859 3891 4373 4405 5734
Size: 20 12 20 198 32 482 32 1329
Cuts at: 5734 5766 5808 5840 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1329 985 907 482 198 135 81
42 32 32 32 32 32 32 32
32 20 20 12
HincII GTy'rAC
Cuts at: 669 1657 1938 2238 3012 4437 4683 669
Size: 988 281 300 774 1425 246 3079
Fragments arranged by size:
3079 1425 988 774 300 281 246
HindIII A'AGCT_T
Cuts at: 748 2035 3423 748
Size: 1287 1388 4418
Fragments arranged by size:
4418 1388 1287
HpaI GTT'AAC
Cuts at: 3012 4683 3012
Size: 1671 5422
KpnI G_GTAC'C
Cuts at: 3989 4418 3989
Size: 429 6664
MfeI C'AATT_G
Cuts at: 2499 4692 2499
Size: 2193 4900
MluI A'CGCG_T
Cuts at: 6969 6969
Size: 7093
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2050 2115 2774 6207 6391 1305
Size: 745 65 659 3433 184 2007
Fragments arranged by size:
3433 2007 745 659 184 65
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5271
Fragments arranged by size:
5271 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 3598 3791 5051 5410 5569 518
Size: 648 1839 593 193 1260 359 159 2042
Fragments arranged by size:
2042 1839 1260 648 593 359 193 159
MspA1I CmG'CkG
Cuts at: 1606 2158 4276 4509 5186 6334 6579 1606
Size: 552 2118 233 677 1148 245 2120
Fragments arranged by size:
2120 2118 1148 677 552 245 233
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6541
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7093
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7093
NotI GC'GGCC_GC
Cuts at: 4540 4540
Size: 7093
NspI r_CATG'y
Cuts at: 1132 1229 3043 3736 4451 5996 1132
Size: 97 1814 693 715 1545 2229
Fragments arranged by size:
2229 1814 1545 715 693 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7093
PciI A'CATG_T
Cuts at: 3732 5992 3732
Size: 2260 4833
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7093
PfoI T'CCnGG_A
Cuts at: 3469 3469
Size: 7093
PmeI GTTT'AAAC
Cuts at: 4397 4397
Size: 7093
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5162 5194 6675 6707 5162
Size: 32 1481 32 5548
Fragments arranged by size:
5548 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2706 1613
Size: 1093 6000
PsiI TTA'TAA
Cuts at: 3681 4663 3681
Size: 982 6111
PstI C_TGCA'G
Cuts at: 830 4445 830
Size: 3615 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7093
SalI G'TCGA_C
Cuts at: 1936 4435 1936
Size: 2499 4594
SbfI CC_TGCA'GG
Cuts at: 4445 4445
Size: 7093
ScaI AGT'ACT
Cuts at: 5341 7053 5341
Size: 1712 5381
SfcI C'TryA_G
Cuts at: 826 1045 4441 5576 6257 6448 826
Size: 219 3396 1135 681 191 1471
Fragments arranged by size:
3396 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7093
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7093
SmaI CCC'GGG
Cuts at: 1872 4420 1872
Size: 2548 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5197 6098 6360 820
Size: 197 926 3254 901 262 1553
Fragments arranged by size:
3254 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4884 493
Size: 4391 2702
SpeI A'CTAG_T
Cuts at: 152 2621 4363 152
Size: 2469 1742 2882
Fragments arranged by size:
2882 2469 1742
SphI G_CATG'C
Cuts at: 1132 4451 1132
Size: 3319 3774
SspI AAT'ATT
Cuts at: 5 52 2315 2686 2851 5017 5
Size: 47 2263 371 165 2166 2081
Fragments arranged by size:
2263 2166 2081 371 165 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7093
StyI C'CwwG_G
Cuts at: 513 1065 4296 4532 513
Size: 552 3231 236 3074
Fragments arranged by size:
3231 3074 552 236
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2631 3546 3958 5120 5137 5290 5475
Size: 1255 1051 915 412 1162 17 153 185
Cuts at: 5475 5920 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1162 1051 915 445 412 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2976 5339 7051
Size: 274 80 33 51 1443 999 2363 1712
Cuts at: 7051 96
Size: 138
Fragments arranged by size:
2363 1712 1443 999 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2731 2763 7053 7085 2731
Size: 32 4290 32 2739
Fragments arranged by size:
4290 2739 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7093
XbaI T'CTAG_A
Cuts at: 4429 4429
Size: 7093
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3793 3793
Size: 7093
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7093
XmaI C'CCGG_G
Cuts at: 1870 4418 1870
Size: 2548 4545
XmnI GAAnn'nnTTC
Cuts at: 2877 3095 4218 5222 2877
Size: 218 1123 1004 4748
Fragments arranged by size:
4748 1123 1004 218
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4901 276
Size: 53 83 186 936 3367 2468
Fragments arranged by size:
3367 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI PvuII RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI