Restriction map for
pFN21AE3300
(Circular) (Six-base) MAPSORT of: pFN21AE3300.seq Check: 6361 from: 1 to: 12370
pFN21AE3300 12370 bp
With 182 enzymes: SgfI *
May 26, 2016 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2360 2534 2594 2708 3059 3209 3410 3776 3863
Size: 174 60 114 351 150 201 366 87
Cuts at: 3863 4088 4115 4148 4313 4346 4922 5003 5030
Size: 225 27 33 165 33 576 81 27
Cuts at: 5030 5150 5489 5603 5741 5774 5975 6125 8712
Size: 120 339 114 138 33 201 150 2587
Cuts at: 8712 8838 9145 2360
Size: 126 307 5585
Fragments arranged by size:
5585 2587 576 366 351 339 307 225
201 201 174 165 150 150 138 126
120 114 114 87 81 60 33 33
33 27 27
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2721 3363 7017 7626
Size: 53 83 186 936 1185 642 3654 609
Cuts at: 7626 8067 10180 278
Size: 441 2113 2468
Fragments arranged by size:
3654 2468 2113 1185 936 642 609 441
186 83 53
AccI GT'mk_AC
Cuts at: 1937 4608 7032 9713 1937
Size: 2671 2424 2681 4594
Fragments arranged by size:
4594 2681 2671 2424
Acc65I G'GTAC_C
Cuts at: 9691 9691
Size: 12370
AclI AA'CG_TT
Cuts at: 1563 10497 10870 1563
Size: 8934 373 3063
Fragments arranged by size:
8934 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3914 4336 4912 6487 6607 7389 7415 8168
Size: 2282 422 576 1575 120 782 26 753
Cuts at: 8168 10433 11817 12085 1632
Size: 2265 1384 268 1917
Fragments arranged by size:
2282 2265 1917 1575 1384 782 753 576
422 268 120 26
AfeI AGC'GCT
Cuts at: 5792 7119 5792
Size: 1327 11043
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 12173
AflIII A'CryG_T
Cuts at: 1203 4329 4418 4478 4905 6035 6414 11269 12246
Size: 3126 89 60 427 1130 379 4855 977
Cuts at: 12246 1203
Size: 1327
Fragments arranged by size:
4855 3126 1327 1130 977 427 379 89
60
AgeI A'CCGG_T
Cuts at: 11201 11201
Size: 12370
AhdI GACnn_n'nnGTC
Cuts at: 5698 9562 11099 5698
Size: 3864 1537 6969
Fragments arranged by size:
6969 3864 1537
AleI CACnn'nnGTG
Cuts at: 1166 2730 3659 4997 5190 5735 6725 6812 7097
Size: 1564 929 1338 193 545 990 87 285
Cuts at: 7097 1166
Size: 6439
Fragments arranged by size:
6439 1564 1338 990 929 545 285 193
87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3122 3455 4472 7566 7913 1184
Size: 1938 333 1017 3094 347 5641
Fragments arranged by size:
5641 3094 1938 1017 347 333
AlwNI CAG_nnn'CTG
Cuts at: 2470 3325 3498 3951 4515 5055 5268 5427 5463
Size: 855 173 453 564 540 213 159 36
Cuts at: 5463 6174 6484 7546 7942 9106 9193 9340 9448
Size: 711 310 1062 396 1164 87 147 108
Cuts at: 9448 10113 11685 2470
Size: 665 1572 3155
Fragments arranged by size:
3155 1572 1164 1062 855 711 665 564
540 453 396 310 213 173 159 147
108 87 36
ApaI G_GGCC'C
Cuts at: 2168 2292 3224 3344 6134 6146 8884 9347 2168
Size: 124 932 120 2790 12 2738 463 5191
Fragments arranged by size:
5191 2790 2738 932 463 124 120 12
ApaLI G'TGCA_C
Cuts at: 3458 3664 4333 4909 5137 5183 5651 9362 10427
Size: 206 669 576 228 46 468 3711 1065
Cuts at: 10427 11583 3458
Size: 1156 4245
Fragments arranged by size:
4245 3711 1156 1065 669 576 468 228
206 46
ApoI r'AATT_y
Cuts at: 1493 6260 8887 9679 9905 10057 12166 1493
Size: 4767 2627 792 226 152 2109 1697
Fragments arranged by size:
4767 2627 2109 1697 792 226 152
AseI AT'TA_AT
Cuts at: 160 1032 10924 160
Size: 872 9892 1606
Fragments arranged by size:
9892 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12370
AvaI C'yCGr_G
Cuts at: 1870 1943 2561 3208 3958 4118 6359 6701 8211
Size: 73 618 647 750 160 2241 342 1510
Cuts at: 8211 8762 9695 1870
Size: 551 933 4545
Fragments arranged by size:
4545 2241 1510 933 750 647 618 551
342 160 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 6037 6070 6037
Size: 33 12337
BamHI G'GATC_C
Cuts at: 4935 9700 10067 4935
Size: 4765 367 7238
Fragments arranged by size:
7238 4765 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2020 2348 2582 2696 2813
Size: 325 217 627 233 328 234 114 117
Cuts at: 2813 3044 3260 3623 3851 3962 4076 4301 4472
Size: 231 216 363 228 111 114 225 171
Cuts at: 4472 4991 5105 5591 5729 5843 5907 5963 6029
Size: 519 114 486 138 114 64 56 66
Cuts at: 6029 7412 7754 8739 8846 8959 8995 9514 9691
Size: 1383 342 985 107 113 36 519 177
Cuts at: 9691 11146 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1383 985 627 519 519 486
363 342 328 325 234 233 231 228
225 217 216 177 171 138 117 114
114 114 114 113 111 107 66 64
56 36
BanII G_rGCy'C
Cuts at: 1391 2168 2292 3158 3224 3308 3344 4634 4766
Size: 777 124 866 66 84 36 1290 132
Cuts at: 4766 4976 5367 5711 6134 6146 6219 6233 6273
Size: 210 391 344 423 12 73 14 40
Cuts at: 6273 6792 7362 7488 8386 8867 8884 9272 9347
Size: 519 570 126 898 481 17 388 75
Cuts at: 9347 9389 9689 1391
Size: 42 300 4072
Fragments arranged by size:
4072 1290 898 866 777 570 519 481
423 391 388 344 300 210 132 126
124 84 75 73 66 42 40 36
17 14 12
BbeI G_GCGC'C
Cuts at: 2024 3048 5911 6033 7758 2024
Size: 1024 2863 122 1725 6636
Fragments arranged by size:
6636 2863 1725 1024 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 6811 7924 928
Size: 971 4912 1113 5374
Fragments arranged by size:
5374 4912 1113 971
BbvCI CC'TCA_GC
Cuts at: 2195 2195
Size: 12370
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4031 4065 5581 5615 5873
Size: 34 50 34 2010 34 1516 34 258
Cuts at: 5873 5907 10561 10595 1903
Size: 34 4654 34 3678
Fragments arranged by size:
4654 3678 2010 1516 258 50 34 34
34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 10262 11472 10262
Size: 1210 11160
BclI T'GATC_A
Cuts at: 1548 1548
Size: 12370
BfrBI ATG'CAT
Cuts at: 3132 6093 7981 8320 3132
Size: 2961 1888 339 7182
Fragments arranged by size:
7182 2961 1888 339
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2103 2397 2580
Size: 107 122 71 631 605 431 294 183
Cuts at: 2580 2712 2745 2928 3558 3735 3783 3849 4122
Size: 132 33 183 630 177 48 66 273
Cuts at: 4122 4347 4581 4644 4695 4809 4923 5100 5151
Size: 225 234 63 51 114 114 177 51
Cuts at: 5151 5265 5409 5637 5775 6018 6081 6183 8018
Size: 114 144 228 138 243 63 102 1835
Cuts at: 8018 9291 10981 136
Size: 1273 1690 1525
Fragments arranged by size:
1835 1690 1525 1273 631 630 605 431
294 273 243 234 228 225 183 183
177 177 144 138 132 122 114 114
114 107 102 71 66 63 63 51
51 48 33
BglII A'GATC_T
Cuts at: 12365 12365
Size: 12370
BlpI GC'TnA_GC
Cuts at: 6791 9271 9787 6791
Size: 2480 516 9374
Fragments arranged by size:
9374 2480 516
Bme1580I G_kGCm'C
Cuts at: 1790 2168 2275 2292 2351 2667 2699 3224 3344
Size: 378 107 17 59 316 32 525 120
Cuts at: 3344 3462 3554 3668 3854 3965 4079 4337 4418
Size: 118 92 114 186 111 114 258 81
Cuts at: 4418 4507 4823 4913 5141 5187 5261 5295 5594
Size: 89 316 90 228 46 74 34 299
Cuts at: 5594 5642 5655 5966 6014 6134 6146 6254 6636
Size: 48 13 311 48 120 12 108 382
Cuts at: 6636 7417 8399 8694 8742 8884 9347 9366 10431
Size: 781 982 295 48 142 463 19 1065
Cuts at: 10431 11587 1790
Size: 1156 2573
Fragments arranged by size:
2573 1156 1065 982 781 525 463 382
378 316 316 311 299 295 258 228
186 142 120 120 118 114 114 111
108 107 92 90 89 81 74 59
48 48 48 46 34 32 19 17
13 12
BmgBI CAC'GTC
Cuts at: 1318 3241 5290 7099 8641 9331 9402 1318
Size: 1923 2049 1809 1542 690 71 4286
Fragments arranged by size:
4286 2049 1923 1809 1542 690 71
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3576 5236 8113
Size: 416 53 83 399 24 2159 1660 2877
Cuts at: 8113 9350 9436 10101 11059 442
Size: 1237 86 665 958 1753
Fragments arranged by size:
2877 2159 1753 1660 1237 958 665 416
399 86 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 12370
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2620 3391 4579 5098 5584 9628 2620
Size: 771 1188 519 486 4044 5362
Fragments arranged by size:
5362 4044 1188 771 519 486
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3811 7058 7478 7709 7868 8057 8633 8851
Size: 2572 3247 420 231 159 189 576 218
Cuts at: 8851 9598 11030 1239
Size: 747 1432 2579
Fragments arranged by size:
3247 2579 2572 1432 747 576 420 231
218 189 159
Bpu10I CC'TnA_GC
Cuts at: 2195 9008 9519 2195
Size: 6813 511 5046
Fragments arranged by size:
6813 5046 511
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7370 8435 8516 10495 11360 11658 7370
Size: 1065 81 1979 865 298 8082
Fragments arranged by size:
8082 1979 1065 865 298 81
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2084 3800 4712 7169 7454 7784 8222
Size: 617 585 1716 912 2457 285 330 438
Cuts at: 8222 11033 882
Size: 2811 2219
Fragments arranged by size:
2811 2457 2219 1716 912 617 585 438
330 285
BsaAI yAC'GTr
Cuts at: 493 1204 2230 2662 4330 4419 4479 4908 6036
Size: 711 1026 432 1668 89 60 429 1128
Cuts at: 6036 7219 10161 493
Size: 1183 2942 2702
Fragments arranged by size:
2942 2702 1668 1183 1128 1026 711 432
429 89 60
BsaBI GATnn'nnATC
Cuts at: 7943 12364 7943
Size: 4421 7949
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2021 2718 3045 3360
Size: 53 83 186 936 488 697 327 315
Cuts at: 3360 3396 3753 5835 5908 6030 6930 7014 7608
Size: 36 357 2082 73 122 900 84 594
Cuts at: 7608 7623 7755 7994 8064 9452 9609 10177 10559
Size: 15 132 239 70 1388 157 568 382
Cuts at: 10559 11194 275
Size: 635 1451
Fragments arranged by size:
2082 1451 1388 936 900 697 635 594
568 488 382 357 327 315 239 186
157 132 122 84 83 73 70 53
36 15
BsaWI w'CCGG_w
Cuts at: 7473 7947 9655 10803 11201 11475 11622 12033 7473
Size: 474 1708 1148 398 274 147 411 7810
Fragments arranged by size:
7810 1708 1148 474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2366 2396 3140 3170 3413
Size: 30 165 30 963 30 744 30 243
Cuts at: 3413 3443 3722 3752 3809 3839 4012 4042 4748
Size: 30 279 30 57 30 173 30 706
Cuts at: 4748 4778 5609 5639 6653 6683 9458 9488 1178
Size: 30 831 30 1014 30 2775 30 4060
Fragments arranged by size:
4060 2775 1014 963 831 744 706 279
243 173 165 57 30 30 30 30
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4847 5188 5564 6356 6887 6890 7060 7496
Size: 3661 341 376 792 531 3 170 436
Cuts at: 7496 7727 8201 9466 1186
Size: 231 474 1265 4090
Fragments arranged by size:
4090 3661 1265 792 531 474 436 376
341 231 170 3
BseYI C'CCAG_C
Cuts at: 2079 2507 3256 3301 4508 5261 5294 5456 6423
Size: 428 749 45 1207 753 33 162 967
Cuts at: 6423 6915 6937 7129 8389 8486 8776 9110 9129
Size: 492 22 192 1260 97 290 334 19
Cuts at: 9129 9150 9347 9497 11573 12069 2079
Size: 21 197 150 2076 496 2380
Fragments arranged by size:
2380 2076 1260 1207 967 753 749 496
492 428 334 290 197 192 162 150
97 45 33 22 21 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3181 3488 4555 4753 4867 4948 5467
Size: 573 1445 307 1067 198 114 81 519
Cuts at: 5467 5746 5879 6457 6788 7082 7187 7190 7748
Size: 279 133 578 331 294 105 3 558
Cuts at: 7748 8084 8360 8483 9038 9164 9179 9353 1163
Size: 336 276 123 555 126 15 174 4180
Fragments arranged by size:
4180 1445 1067 578 573 558 555 519
336 331 307 294 279 276 198 174
133 126 123 114 105 81 15 3
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6717 6868 7230 7728 8791 9820
Size: 275 186 4716 151 362 498 1063 1029
Cuts at: 9820 10581 11609 1540
Size: 761 1028 2301
Fragments arranged by size:
4716 2301 1063 1029 1028 761 498 362
275 186 151
BsiHKAI G_wGCw'C
Cuts at: 3254 3462 3668 3827 4337 4913 4976 5141 5187
Size: 208 206 159 510 576 63 165 46
Cuts at: 5187 5453 5627 5655 6792 8145 9014 9272 9366
Size: 266 174 28 1137 1353 869 258 94
Cuts at: 9366 9525 10431 10516 11587 3254
Size: 159 906 85 1071 4037
Fragments arranged by size:
4037 1353 1137 1071 906 869 576 510
266 258 208 206 174 165 159 159
94 85 63 46 28
BsmI GAATG_Cn'
Cuts at: 6155 7106 9580 9651 9881 9974 6155
Size: 951 2474 71 230 93 8551
Fragments arranged by size:
8551 2474 951 230 93 71
BsmBI CGTCTCn'nnnn_
Cuts at: 3379 7685 8825 9595 9616 3379
Size: 4306 1140 770 21 6133
Fragments arranged by size:
6133 4306 1140 770 21
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2168 2275 2292 2351 2667 2699 3158
Size: 399 378 107 17 59 316 32 459
Cuts at: 3158 3224 3254 3308 3344 3462 3554 3668 3827
Size: 66 30 54 36 118 92 114 159
Cuts at: 3827 3854 3965 4079 4337 4418 4507 4634 4766
Size: 27 111 114 258 81 89 127 132
Cuts at: 4766 4823 4913 4976 5141 5187 5261 5295 5367
Size: 57 90 63 165 46 74 34 72
Cuts at: 5367 5453 5594 5627 5642 5655 5711 5966 6014
Size: 86 141 33 15 13 56 255 48
Cuts at: 6014 6134 6146 6219 6233 6254 6273 6636 6792
Size: 120 12 73 14 21 19 363 156
Cuts at: 6792 7362 7417 7488 8145 8386 8399 8694 8742
Size: 570 55 71 657 241 13 295 48
Cuts at: 8742 8867 8884 9014 9272 9347 9366 9389 9525
Size: 125 17 130 258 75 19 23 136
Cuts at: 9525 9689 10431 10516 11587 1391
Size: 164 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 657 570 459 399 378
363 316 295 258 258 255 241 165
164 159 156 141 136 132 130 127
125 120 118 114 114 111 107 92
90 89 86 85 81 75 74 73
72 71 66 63 59 57 56 55
54 48 48 46 36 34 33 32
30 27 23 21 19 19 17 17
15 14 13 13 12
BspEI T'CCGG_A
Cuts at: 7947 9655 12033 7947
Size: 1708 2378 8284
Fragments arranged by size:
8284 2378 1708
BspHI T'CATG_A
Cuts at: 7659 10259 11989 7659
Size: 2600 1730 8040
Fragments arranged by size:
8040 2600 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2102 2360 2367 2534 2594 2708 2945 3059
Size: 1258 258 7 167 60 114 237 114
Cuts at: 3059 3209 3410 3776 3863 4088 4115 4148 4313
Size: 150 201 366 87 225 27 33 165
Cuts at: 4313 4346 4922 5003 5030 5150 5489 5603 5741
Size: 33 576 81 27 120 339 114 138
Cuts at: 5741 5774 5975 6065 6125 7569 8712 8838 9145
Size: 33 201 90 60 1444 1143 126 307
Cuts at: 9145 9201 9725 844
Size: 56 524 3489
Fragments arranged by size:
3489 1444 1258 1143 576 524 366 339
307 258 237 225 201 201 167 165
150 138 126 120 114 114 114 90
87 81 60 60 56 33 33 33
27 27 7
BsrBI CCG'CTC
Cuts at: 1394 1823 2014 2653 7657 8419 8952 10257 1394
Size: 429 191 639 5004 762 533 1305 3507
Fragments arranged by size:
5004 3507 1305 762 639 533 429 191
BsrDI GCAATG_nn'
Cuts at: 65 1248 10865 11039 65
Size: 1183 9617 174 1396
Fragments arranged by size:
9617 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2529 2753 4638 4694 4737 5273 5522
Size: 110 719 224 1885 56 43 536 249
Cuts at: 5522 6539 6716 7050 7195 7284 7524 7723 8790
Size: 1017 177 334 145 89 240 199 1067
Cuts at: 8790 11014 11201 1700
Size: 2224 187 2869
Fragments arranged by size:
2869 2224 1885 1067 1017 719 536 334
249 240 224 199 187 177 145 110
89 56 43
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 12370
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 12370
BssSI C'ACGA_G
Cuts at: 2061 2726 10430 11442 2061
Size: 665 7704 1012 2989
Fragments arranged by size:
7704 2989 1012 665
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2205 2258 3498 3612 3951 4062 4176 4890
Size: 1411 53 1240 114 339 111 114 714
Cuts at: 4890 5055 5949 6174 6192 6484 7060 8568 9193
Size: 165 894 225 18 292 576 1508 625
Cuts at: 9193 9226 794
Size: 33 3938
Fragments arranged by size:
3938 1508 1411 1240 894 714 625 576
339 292 225 165 114 114 111 53
33 18
BstBI TT'CG_AA
Cuts at: 11174 11174
Size: 12370
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 12370
BstXI CCAn_nnnn'nTGG
Cuts at: 3406 3634 7099 7966 8708 9355 3406
Size: 228 3465 867 742 647 6421
Fragments arranged by size:
6421 3465 867 742 647 228
BstYI r'GATC_y
Cuts at: 1972 4935 7938 9700 10067 10452 10469 11921 12007
Size: 2963 3003 1762 367 385 17 1452 86
Cuts at: 12007 12019 12365 1972
Size: 12 346 1977
Fragments arranged by size:
3003 2963 1977 1762 1452 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 7314 7314
Size: 12370
BtgI C'CryG_G
Cuts at: 513 1065 2515 2575 2709 2950 3040 3091 3555
Size: 552 1450 60 134 241 90 51 464
Cuts at: 3555 3844 4183 4578 4666 5530 5633 5839 5973
Size: 289 339 395 88 864 103 206 134
Cuts at: 5973 5992 7008 7018 7179 7377 7488 7575 7633
Size: 19 1016 10 161 198 111 87 58
Cuts at: 7633 8022 8601 8623 513
Size: 389 579 22 4260
Fragments arranged by size:
4260 1450 1016 864 579 552 464 395
389 339 289 241 206 198 161 134
134 111 103 90 88 87 60 58
51 22 19 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2086 2185 8287 487
Size: 683 56 860 99 6102 4570
Fragments arranged by size:
6102 4570 860 683 99 56
BtsI GCAGTG_nn'
Cuts at: 2497 3460 3688 3799 4684 5392 5878 6407 6494
Size: 963 228 111 885 708 486 529 87
Cuts at: 6494 6634 7061 7082 7535 8654 9886 10680 10700
Size: 140 427 21 453 1119 1232 794 20
Cuts at: 10700 2497
Size: 4167
Fragments arranged by size:
4167 1232 1119 963 885 794 708 529
486 453 427 228 140 111 87 21
20
ClaI AT'CG_AT
Cuts at: 1554 3705 3981 4041 1554
Size: 2151 276 60 9883
Fragments arranged by size:
9883 2151 276 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2530 2565 8912 8947 529
Size: 35 1966 35 6347 35 3952
Fragments arranged by size:
6347 3952 1966 35 35 35
DraI TTT'AAA
Cuts at: 9674 9674
Size: 12370
DraIII CAC_nnn'GTG
Cuts at: 2233 5536 7928 9142 2233
Size: 3303 2392 1214 5461
Fragments arranged by size:
5461 3303 2392 1214
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7628 11377 809
Size: 507 6312 3749 1802
Fragments arranged by size:
6312 3749 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 2461 2551
Size: 54 1425 50 275 18 291 340 90
Cuts at: 2551 2755 3094 3511 5520 6162 6511 6714 6838
Size: 204 339 417 2009 642 349 203 124
Cuts at: 6838 6865 7101 7227 7297 7725 7806 8019 8205
Size: 27 236 126 70 428 81 213 186
Cuts at: 8205 8754 8788 9123 9283 9817 10706 12335 8
Size: 549 34 335 160 534 889 1629 43
Fragments arranged by size:
2009 1629 1425 889 642 549 534 428
417 349 340 339 335 291 275 236
213 204 203 186 160 126 124 90
81 70 54 50 43 34 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 6714 6865 7227 7725 8788 9817 1537
Size: 275 4902 151 362 498 1063 1029 4090
Fragments arranged by size:
4902 4090 1063 1029 498 362 275 151
EarI CTCTTCn'nnn_
Cuts at: 1353 6554 7704 10300 1353
Size: 5201 1150 2596 3423
Fragments arranged by size:
5201 3423 2596 1150
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7006 8083 10957 11331 11477 387
Size: 1064 138 5417 1077 2874 374 146 1280
Fragments arranged by size:
5417 2874 1280 1077 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4974 6790 9270 4974
Size: 1816 2480 8074
Fragments arranged by size:
8074 2480 1816
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3811 3914 4336 4912 6487 6607 7058
Size: 393 2179 103 422 576 1575 120 451
Cuts at: 7058 7389 7415 7478 7709 7868 8057 8168 8633
Size: 331 26 63 231 159 189 111 465
Cuts at: 8633 8851 9598 10433 11030 11817 12085 1239
Size: 218 747 835 597 787 268 1524
Fragments arranged by size:
2179 1575 1524 835 787 747 597 576
465 451 422 393 331 268 231 218
189 159 120 111 103 63 26
EcoNI CCTnn'n_nnAGG
Cuts at: 4148 7367 7847 4148
Size: 3219 480 8671
Fragments arranged by size:
8671 3219 480
EcoO109I rG'GnC_Cy
Cuts at: 2154 2179 2289 2934 3341 3908 4188 5434 6131
Size: 25 110 645 407 567 280 1246 697
Cuts at: 6131 6143 6207 7453 8041 8527 8880 8881 9002
Size: 12 64 1246 588 486 353 1 121
Cuts at: 9002 9019 9103 9344 2154
Size: 17 84 241 5180
Fragments arranged by size:
5180 1246 1246 697 645 588 567 486
407 353 280 241 121 110 84 64
25 17 12 1
EcoRI G'AATT_C
Cuts at: 9679 10057 9679
Size: 378 11992
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 6637 6637
Size: 12370
FspI TGC'GCA
Cuts at: 5319 6761 6884 7565 8363 10876 5319
Size: 1442 123 681 798 2513 6813
Fragments arranged by size:
6813 2513 1442 798 681 123
HaeII r_GCGC'y
Cuts at: 1678 2024 2042 3030 3048 5794 5911 6033 7121
Size: 346 18 988 18 2746 117 122 1088
Cuts at: 7121 7285 7758 7843 8777 9078 11517 1678
Size: 164 473 85 934 301 2439 2531
Fragments arranged by size:
2746 2531 2439 1088 988 934 473 346
301 164 122 117 85 18 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3236 3268 3339 3371 4262
Size: 32 135 32 1664 32 71 32 891
Cuts at: 4262 4294 4376 4408 5435 5467 5684 5716 5804
Size: 32 82 32 1027 32 217 32 88
Cuts at: 5804 5836 6206 6238 8630 8662 8921 8953 9548
Size: 32 370 32 2392 32 259 32 595
Cuts at: 9548 9580 11011 11043 11085 11117 1373
Size: 32 1431 32 42 32 2626
Fragments arranged by size:
2626 2392 1664 1431 1027 891 595 370
259 217 135 88 82 71 42 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2542 2977 3364 3472 4294 4609
Size: 988 281 604 435 387 108 822 315
Cuts at: 4609 5335 5692 5809 7606 8068 9714 9960 669
Size: 726 357 117 1797 462 1646 246 3079
Fragments arranged by size:
3079 1797 1646 988 822 726 604 462
435 387 357 315 281 246 117 108
HindIII A'AGCT_T
Cuts at: 748 748
Size: 12370
HpaI GTT'AAC
Cuts at: 9960 9960
Size: 12370
KasI G'GCGC_C
Cuts at: 2020 3044 5907 6029 7754 2020
Size: 1024 2863 122 1725 6636
Fragments arranged by size:
6636 2863 1725 1024 122
KpnI G_GTAC'C
Cuts at: 9695 9695
Size: 12370
MfeI C'AATT_G
Cuts at: 9969 9969
Size: 12370
MluI A'CGCG_T
Cuts at: 6414 12246 6414
Size: 5832 6538
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2466 3183 4305 4419 6006 7761 9609 9618
Size: 1161 717 1122 114 1587 1755 1848 9
Cuts at: 9618 11484 11668 1305
Size: 1866 184 2007
Fragments arranged by size:
2007 1866 1848 1755 1587 1161 1122 717
184 114 9
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2463 2553 7299 9125 10
Size: 54 1425 343 631 90 4746 1826 3255
Fragments arranged by size:
4746 3255 1826 1425 631 343 90 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 3060 3437 3659 4997 5190 5735
Size: 648 1564 330 377 222 1338 193 545
Cuts at: 5735 6725 6812 7097 7217 7548 9629 10328 10687
Size: 990 87 285 120 331 2081 699 359
Cuts at: 10687 10846 518
Size: 159 2042
Fragments arranged by size:
2081 2042 1564 1338 990 699 648 545
377 359 331 330 285 222 193 159
120 87
MspA1I CmG'CkG
Cuts at: 1606 2268 2388 2613 2850 2905 3516 4113 4356
Size: 662 120 225 237 55 611 597 243
Cuts at: 4356 4680 4932 5250 5265 5400 5760 5913 5994
Size: 324 252 318 15 135 360 153 81
Cuts at: 5994 6334 6757 7020 7204 7556 7635 7677 7897
Size: 340 423 263 184 352 79 42 220
Cuts at: 7897 8374 8389 8874 9052 9282 9786 10463 11611
Size: 477 15 485 178 230 504 677 1148
Cuts at: 11611 11856 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 662 611 597 504 485
477 423 360 352 340 324 318 263
252 245 243 237 230 225 220 184
178 153 135 120 81 79 55 42
15 15
NaeI GCC'GGC
Cuts at: 2531 4640 4696 4739 5524 6541 6718 7197 7286
Size: 2109 56 43 785 1017 177 479 89
Cuts at: 7286 7725 8792 2531
Size: 439 1067 6109
Fragments arranged by size:
6109 2109 1067 1017 785 479 439 177
89 56 43
NarI GG'CG_CC
Cuts at: 2021 3045 5908 6030 7755 2021
Size: 1024 2863 122 1725 6636
Fragments arranged by size:
6636 2863 1725 1024 122
NcoI C'CATG_G
Cuts at: 513 1065 2515 3091 7008 7575 8623 513
Size: 552 1450 576 3917 567 1048 4260
Fragments arranged by size:
4260 3917 1450 1048 576 567 552
NdeI CA'TA_TG
Cuts at: 387 8280 387
Size: 7893 4477
NgoMIV G'CCGG_C
Cuts at: 2529 4638 4694 4737 5522 6539 6716 7195 7284
Size: 2109 56 43 785 1017 177 479 89
Cuts at: 7284 7723 8790 2529
Size: 439 1067 6109
Fragments arranged by size:
6109 2109 1067 1017 785 479 439 177
89 56 43
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 12370
NotI GC'GGCC_GC
Cuts at: 7227 9817 7227
Size: 2590 9780
NsiI A_TGCA'T
Cuts at: 3134 6095 7983 8322 3134
Size: 2961 1888 339 7182
Fragments arranged by size:
7182 2961 1888 339
NspI r_CATG'y
Cuts at: 1132 1229 2226 3132 3281 3442 4027 6093 6698
Size: 97 997 906 149 161 585 2066 605
Cuts at: 6698 7570 7904 7981 8035 8320 8593 9634 9728
Size: 872 334 77 54 285 273 1041 94
Cuts at: 9728 11273 1132
Size: 1545 2229
Fragments arranged by size:
2229 2066 1545 1041 997 906 872 605
585 334 285 273 161 149 97 94
77 54
PasI CC'CwG_GG
Cuts at: 1348 2275 4232 5149 7594 1348
Size: 927 1957 917 2445 6124
Fragments arranged by size:
6124 2445 1957 927 917
PciI A'CATG_T
Cuts at: 11269 11269
Size: 12370
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2233 3241 4584 5268 6286 1108
Size: 1125 1008 1343 684 1018 7192
Fragments arranged by size:
7192 1343 1125 1018 1008 684
PfoI T'CCnGG_A
Cuts at: 2788 5702 6285 7872 2788
Size: 2914 583 1587 7286
Fragments arranged by size:
7286 2914 1587 583
PmeI GTTT'AAAC
Cuts at: 9674 9674
Size: 12370
PmlI CAC'GTG
Cuts at: 2230 4419 4479 4908 6036 2230
Size: 2189 60 429 1128 8564
Fragments arranged by size:
8564 2189 1128 429 60
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 10439 10471 11952 11984 10439
Size: 32 1481 32 10825
Fragments arranged by size:
10825 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2179 2934 3908 5434 6207 7453 8041 8527 2179
Size: 755 974 1526 773 1246 588 486 6022
Fragments arranged by size:
6022 1526 1246 974 773 755 588 486
PshAI GACnn'nnGTC
Cuts at: 1613 2784 1613
Size: 1171 11199
PsiI TTA'TAA
Cuts at: 9940 9940
Size: 12370
PspOMI G'GGCC_C
Cuts at: 2164 2288 3220 3340 6130 6142 8880 9343 2164
Size: 124 932 120 2790 12 2738 463 5191
Fragments arranged by size:
5191 2790 2738 932 463 124 120 12
PstI C_TGCA'G
Cuts at: 830 2267 2672 3002 3233 3330 4520 6395 6402
Size: 1437 405 330 231 97 1190 1875 7
Cuts at: 6402 6489 7676 8949 9033 9198 9231 9722 830
Size: 87 1187 1273 84 165 33 491 3478
Fragments arranged by size:
3478 1875 1437 1273 1190 1187 491 405
330 231 165 97 87 84 33 7
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 12370
PvuII CAG'CTG
Cuts at: 2268 2905 5760 6757 7204 8374 2268
Size: 637 2855 997 447 1170 6264
Fragments arranged by size:
6264 2855 1170 997 637 447
SacI G_AGCT'C
Cuts at: 4976 6792 9272 4976
Size: 1816 2480 8074
Fragments arranged by size:
8074 2480 1816
SacII CC_GC'GG
Cuts at: 5995 7021 7636 5995
Size: 1026 615 10729
Fragments arranged by size:
10729 1026 615
SalI G'TCGA_C
Cuts at: 1936 4607 9712 1936
Size: 2671 5105 4594
Fragments arranged by size:
5105 4594 2671
SbfI CC_TGCA'GG
Cuts at: 9722 9722
Size: 12370
ScaI AGT'ACT
Cuts at: 2876 6500 6608 10618 12330 2876
Size: 3624 108 4010 1712 2916
Fragments arranged by size:
4010 3624 2916 1712 108
SexAI A'CCwGG_T
Cuts at: 5436 5742 8355 9138 5436
Size: 306 2613 783 8668
Fragments arranged by size:
8668 2613 783 306
SfcI C'TryA_G
Cuts at: 826 1045 2263 2668 2998 3229 3326 4516 4699
Size: 219 1218 405 330 231 97 1190 183
Cuts at: 4699 5131 5755 6292 6391 6398 6485 7672 8945
Size: 432 624 537 99 7 87 1187 1273
Cuts at: 8945 9029 9194 9227 9509 9718 10853 11534 11725
Size: 84 165 33 282 209 1135 681 191
Cuts at: 11725 826
Size: 1471
Fragments arranged by size:
1471 1273 1218 1190 1187 1135 681 624
537 432 405 330 282 231 219 209
191 183 165 99 97 87 84 33
7
SfiI GGCCn_nnn'nGGCC
Cuts at: 9291 9291
Size: 12370
SfoI GGC'GCC
Cuts at: 2022 3046 5909 6031 7756 2022
Size: 1024 2863 122 1725 6636
Fragments arranged by size:
6636 2863 1725 1024 122
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 12370
SgrAI Cr'CCGG_yG
Cuts at: 1700 7195 7284 1700
Size: 5495 89 6786
Fragments arranged by size:
6786 5495 89
SmaI CCC'GGG
Cuts at: 1872 2563 8213 9697 1872
Size: 691 5650 1484 4545
Fragments arranged by size:
5650 4545 1484 691
SmlI C'TyrA_G
Cuts at: 820 1017 1943 7385 8450 8531 10474 11375 11637
Size: 197 926 5442 1065 81 1943 901 262
Cuts at: 11637 820
Size: 1553
Fragments arranged by size:
5442 1943 1553 1065 926 901 262 197
81
SnaBI TAC'GTA
Cuts at: 493 10161 493
Size: 9668 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 12370
SphI G_CATG'C
Cuts at: 1132 3132 3281 3442 7904 7981 8035 8320 8593
Size: 2000 149 161 4462 77 54 285 273
Cuts at: 8593 9634 9728 1132
Size: 1041 94 3774
Fragments arranged by size:
4462 3774 2000 1041 285 273 161 149
94 77 54
SrfI GCCC'GGGC
Cuts at: 2563 2563
Size: 12370
SspI AAT'ATT
Cuts at: 5 52 10294 5
Size: 47 10242 2081
Fragments arranged by size:
10242 2081 47
StuI AGG'CCT
Cuts at: 1517 2138 2483 6953 7067 8499 9662 1517
Size: 621 345 4470 114 1432 1163 4225
Fragments arranged by size:
4470 4225 1432 1163 621 345 114
StyI C'CwwG_G
Cuts at: 513 1065 2515 3091 7008 7575 8235 8523 8623
Size: 552 1450 576 3917 567 660 288 100
Cuts at: 8623 9259 9809 513
Size: 636 550 3074
Fragments arranged by size:
3917 3074 1450 660 636 576 567 552
550 288 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2521 2645 2738 2963 3248 4224 4679
Size: 1255 941 124 93 225 285 976 455
Cuts at: 4679 4736 5255 5470 5774 5861 8738 8768 9143
Size: 57 519 215 304 87 2877 30 375
Cuts at: 9143 10397 10414 10567 10752 11197 325
Size: 1254 17 153 185 445 1498
Fragments arranged by size:
2877 1498 1255 1254 976 941 519 455
445 375 304 285 225 215 185 153
124 93 87 57 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2376 2874 6498
Size: 274 80 33 51 1443 399 498 3624
Cuts at: 6498 6606 8349 10616 12328 96
Size: 108 1743 2267 1712 138
Fragments arranged by size:
3624 2267 1743 1712 1443 498 399 274
138 108 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2366 2398 2496 2528 3413 3445 3722 3754 4237
Size: 32 98 32 885 32 277 32 483
Cuts at: 4237 4269 4634 4666 4927 4959 6569 6601 6653
Size: 32 365 32 261 32 1610 32 52
Cuts at: 6653 6685 9458 9490 12330 12362 2366
Size: 32 2773 32 2840 32 2374
Fragments arranged by size:
2840 2773 2374 1610 885 483 365 277
261 98 52 32 32 32 32 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3752 1316
Size: 2436 9934
XbaI T'CTAG_A
Cuts at: 9706 9706
Size: 12370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4483 5156 7033 7843 8242 8437 8764 8857 9118
Size: 673 1877 810 399 195 327 93 261
Cuts at: 9118 9286 4483
Size: 168 7567
Fragments arranged by size:
7567 1877 810 673 399 327 261 195
168 93
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 12370
XmaI C'CCGG_G
Cuts at: 1870 2561 8211 9695 1870
Size: 691 5650 1484 4545
Fragments arranged by size:
5650 4545 1484 691
XmnI GAAnn'nnTTC
Cuts at: 6500 10499 6500
Size: 3999 8371
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2719 3361 7015 7624
Size: 53 83 186 936 1185 642 3654 609
Cuts at: 7624 8065 10178 276
Size: 441 2113 2468
Fragments arranged by size:
3654 2468 2113 1185 936 642 609 441
186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BsiWI BstZ17I EcoRV FseI FspAI
NruI PacI PspXI PsrI RsrII SanDI SapI SwaI