Restriction map for
pFN21AE3293
(Circular) (Six-base) MAPSORT of: pFN21AE3293.seq Check: 9428 from: 1 to: 7000
pFN21AE3293 7000 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3124 3648 3124
Size: 524 6476
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4164 4810 278
Size: 53 83 186 936 2628 646 2468
Fragments arranged by size:
2628 2468 936 646 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3162 4343 1937
Size: 1225 1181 4594
Fragments arranged by size:
4594 1225 1181
Acc65I G'GTAC_C
Cuts at: 4321 4321
Size: 7000
AclI AA'CG_TT
Cuts at: 1563 5127 5500 1563
Size: 3564 373 3063
Fragments arranged by size:
3564 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2086 3011 3035 3665 5063 6447 6715 1632
Size: 454 925 24 630 1398 1384 268 1917
Fragments arranged by size:
1917 1398 1384 925 630 454 268 24
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6803
AflIII A'CryG_T
Cuts at: 1203 5899 6876 1203
Size: 4696 977 1327
Fragments arranged by size:
4696 1327 977
AgeI A'CCGG_T
Cuts at: 5831 5831
Size: 7000
AhdI GACnn_n'nnGTC
Cuts at: 5729 5729
Size: 7000
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7000
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3755 3918 4205 1184
Size: 2571 163 287 3979
Fragments arranged by size:
3979 2571 287 163
AlwNI CAG_nnn'CTG
Cuts at: 2133 2284 2349 2986 4743 6315 2133
Size: 151 65 637 1757 1572 2818
Fragments arranged by size:
2818 1757 1572 637 151 65
ApaI G_GGCC'C
Cuts at: 3025 3295 3025
Size: 270 6730
ApaLI G'TGCA_C
Cuts at: 5057 6213 5057
Size: 1156 5844
ApoI r'AATT_y
Cuts at: 1493 2484 3046 4309 4535 4687 6796 1493
Size: 991 562 1263 226 152 2109 1697
Fragments arranged by size:
2109 1697 1263 991 562 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5554 160
Size: 872 4522 1606
Fragments arranged by size:
4522 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 3055 4325 1870
Size: 73 1011 101 1270 4545
Fragments arranged by size:
4545 1270 1011 101 73
BamHI G'GATC_C
Cuts at: 4330 4697 4330
Size: 367 6633
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2079 4148 4321 5776 618
Size: 325 217 627 292 2069 173 1455 1842
Fragments arranged by size:
2069 1842 1455 627 325 292 217 173
BanII G_rGCy'C
Cuts at: 1391 3025 3295 4319 1391
Size: 1634 270 1024 4072
Fragments arranged by size:
4072 1634 1024 270
BbeI G_GCGC'C
Cuts at: 2083 4152 2083
Size: 2069 4931
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2041 928
Size: 971 142 5887
Fragments arranged by size:
5887 971 142
BbvCI CC'TCA_GC
Cuts at: 3480 3480
Size: 7000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3218 3252 5191 5225 1903
Size: 34 1281 34 1939 34 3678
Fragments arranged by size:
3678 1939 1281 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3182 4892 6102 3182
Size: 1710 1210 4080
Fragments arranged by size:
4080 1710 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7000
BfrBI ATG'CAT
Cuts at: 2476 2476
Size: 7000
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4097 5611 136
Size: 107 122 71 631 605 2425 1514 1525
Fragments arranged by size:
2425 1525 1514 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6995 6995
Size: 7000
BlpI GC'TnA_GC
Cuts at: 2567 3570 4417 2567
Size: 1003 847 5150
Fragments arranged by size:
5150 1003 847
Bme1580I G_kGCm'C
Cuts at: 1790 3025 3241 3295 5061 6217 1790
Size: 1235 216 54 1766 1156 2573
Fragments arranged by size:
2573 1766 1235 1156 216 54
BmgBI CAC'GTC
Cuts at: 1318 3166 1318
Size: 1848 5152
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4731 5689 442
Size: 416 53 83 399 24 3314 958 1753
Fragments arranged by size:
3314 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7000
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2568 2568
Size: 7000
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2342 2615 3842 5660 1239
Size: 1103 273 1227 1818 2579
Fragments arranged by size:
2579 1818 1227 1103 273
Bpu10I CC'TnA_GC
Cuts at: 2360 3480 3743 2360
Size: 1120 263 5617
Fragments arranged by size:
5617 1120 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 3782 4091 5125 5990 6288 2426
Size: 1356 309 1034 865 298 3138
Fragments arranged by size:
3138 1356 1034 865 309 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2375 3451 5663 882
Size: 617 876 1076 2212 2219
Fragments arranged by size:
2219 2212 1076 876 617
BsaAI yAC'GTr
Cuts at: 493 1204 4791 493
Size: 711 3587 2702
Fragments arranged by size:
3587 2702 711
BsaBI GATnn'nnATC
Cuts at: 2708 6994 2708
Size: 4286 2714
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2080 2181 4149 4161
Size: 53 83 186 936 547 101 1968 12
Cuts at: 4161 4807 5189 5824 275
Size: 646 382 635 1451
Fragments arranged by size:
1968 1451 936 646 635 547 382 186
101 83 53 12
BsaWI w'CCGG_w
Cuts at: 5433 5831 6105 6252 6663 5433
Size: 398 274 147 411 5770
Fragments arranged by size:
5770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4185 4215 1178
Size: 30 165 30 2782 30 3963
Fragments arranged by size:
3963 2782 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3932 4024 1186
Size: 2746 92 4162
Fragments arranged by size:
4162 2746 92
BseYI C'CCAG_C
Cuts at: 2032 2775 2805 3288 4152 6203 6699 2032
Size: 743 30 483 864 2051 496 2333
Fragments arranged by size:
2333 2051 864 743 496 483 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 3284 3393 3581 3680 3878 3888
Size: 573 654 894 109 188 99 198 10
Cuts at: 3888 4165 1163
Size: 277 3998
Fragments arranged by size:
3998 894 654 573 277 198 188 109
99 10
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3040 4450 5211 6239 1540
Size: 275 186 1039 1410 761 1028 2301
Fragments arranged by size:
2301 1410 1039 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3868 4016 5061 5146 6217 3868
Size: 148 1045 85 1071 4651
Fragments arranged by size:
4651 1071 1045 148 85
BsmI GAATG_Cn'
Cuts at: 4511 4604 4511
Size: 93 6907
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3025 3241 3295 3868 4016 4319 5061
Size: 399 1235 216 54 573 148 303 742
Cuts at: 5061 5146 6217 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1235 1071 742 573 399 303 216
148 85 54
BspEI T'CCGG_A
Cuts at: 6663 6663
Size: 7000
BspHI T'CATG_A
Cuts at: 4889 6619 4889
Size: 1730 5270
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3124 3477 3648 4355 844
Size: 2280 353 171 707 3489
Fragments arranged by size:
3489 2280 707 353 171
BsrBI CCG'CTC
Cuts at: 1394 1823 3613 3656 3681 4887 1394
Size: 429 1790 43 25 1206 3507
Fragments arranged by size:
3507 1790 1206 429 43 25
BsrDI GCAATG_nn'
Cuts at: 65 1248 5495 5669 65
Size: 1183 4247 174 1396
Fragments arranged by size:
4247 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5644 5831 1700
Size: 110 3834 187 2869
Fragments arranged by size:
3834 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2631 96
Size: 2535 4465
BssHII G'CGCG_C
Cuts at: 1925 3335 1925
Size: 1410 5590
BssSI C'ACGA_G
Cuts at: 3155 5060 6072 3155
Size: 1905 1012 4083
Fragments arranged by size:
4083 1905 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3257 794
Size: 2463 4537
BstBI TT'CG_AA
Cuts at: 5804 5804
Size: 7000
BstEII G'GTnAC_C
Cuts at: 1184 3091 1184
Size: 1907 5093
BstXI CCAn_nnnn'nTGG
Cuts at: 2512 3274 2512
Size: 762 6238
BstYI r'GATC_y
Cuts at: 1972 4330 4697 5082 5099 6551 6637 6649 6995
Size: 2358 367 385 17 1452 86 12 346
Cuts at: 6995 1972
Size: 1977
Fragments arranged by size:
2358 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3352 3910 513
Size: 552 939 1348 558 3603
Fragments arranged by size:
3603 1348 939 558 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6261
Fragments arranged by size:
6261 683 56
BtsI GCAGTG_nn'
Cuts at: 2873 3130 3296 3330 3593 3692 3699 3935 3950
Size: 257 166 34 263 99 7 236 15
Cuts at: 3950 4516 5310 5330 2873
Size: 566 794 20 4543
Fragments arranged by size:
4543 794 566 263 257 236 166 99
34 20 15 7
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7000
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6965
DraI TTT'AAA
Cuts at: 4304 4304
Size: 7000
DraIII CAC_nnn'GTG
Cuts at: 2254 3257 2254
Size: 1003 5997
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6007 809
Size: 507 4691 1802
Fragments arranged by size:
4691 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 2307 2391
Size: 54 1425 50 275 18 258 219 84
Cuts at: 2391 2762 2916 3504 3517 4098 4257 4447 5336
Size: 371 154 588 13 581 159 190 889
Cuts at: 5336 6965 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 588 581 371 275 258
219 190 159 154 84 54 50 43
18 13
EagI C'GGCC_G
Cuts at: 1537 1812 4447 1537
Size: 275 2635 4090
Fragments arranged by size:
4090 2635 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2819 3402 3641 4930 1353
Size: 1466 583 239 1289 3423
Fragments arranged by size:
3423 1466 1289 583 239
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2152 5587 5961 6107 387
Size: 1064 138 563 3435 374 146 1280
Fragments arranged by size:
3435 1280 1064 563 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2086 2342 2615 3011 3035 3665 3842
Size: 393 454 256 273 396 24 630 177
Cuts at: 3842 5063 5660 6447 6715 1239
Size: 1221 597 787 268 1524
Fragments arranged by size:
1524 1221 787 630 597 454 396 393
273 268 256 177 24
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 3473 2324
Size: 1149 5851
EcoO109I rG'GnC_Cy
Cuts at: 2608 2615 2815 2911 3022 3292 3357 2608
Size: 7 200 96 111 270 65 6251
Fragments arranged by size:
6251 270 200 111 96 65 7
EcoRI G'AATT_C
Cuts at: 4309 4687 4309
Size: 378 6622
FspI TGC'GCA
Cuts at: 5506 5506
Size: 7000
HaeII r_GCGC'y
Cuts at: 1678 2083 2134 4152 6147 1678
Size: 405 51 2018 1995 2531
Fragments arranged by size:
2531 2018 1995 405 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2984 3016 3817 3849 5641
Size: 32 135 32 1412 32 801 32 1792
Cuts at: 5641 5673 5715 5747 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1792 1412 801 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4344 4590 669
Size: 988 281 2406 246 3079
Fragments arranged by size:
3079 2406 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7000
HpaI GTT'AAC
Cuts at: 4590 4590
Size: 7000
KasI G'GCGC_C
Cuts at: 2079 4148 2079
Size: 2069 4931
KpnI G_GTAC'C
Cuts at: 4325 4325
Size: 7000
MfeI C'AATT_G
Cuts at: 2643 4599 2643
Size: 1956 5044
MluI A'CGCG_T
Cuts at: 6876 6876
Size: 7000
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3060 3543 3658 6114 6298 1305
Size: 1755 483 115 2456 184 2007
Fragments arranged by size:
2456 2007 1755 483 184 115
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2090 2309 2393 2764 2918
Size: 54 1425 343 258 219 84 371 154
Cuts at: 2918 3519 4259 10
Size: 601 740 2751
Fragments arranged by size:
2751 1425 740 601 371 343 258 219
154 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2528 2699 4053 4958 5317 5476 518
Size: 648 1362 171 1354 905 359 159 2042
Fragments arranged by size:
2042 1362 1354 905 648 359 171 159
MspA1I CmG'CkG
Cuts at: 1606 2094 3707 4156 4416 5093 6241 6486 1606
Size: 488 1613 449 260 677 1148 245 2120
Fragments arranged by size:
2120 1613 1148 677 488 449 260 245
NarI GG'CG_CC
Cuts at: 2080 4149 2080
Size: 2069 4931
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5509
Fragments arranged by size:
5509 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7000
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7000
NotI GC'GGCC_GC
Cuts at: 4447 4447
Size: 7000
NsiI A_TGCA'T
Cuts at: 2478 2478
Size: 7000
NspI r_CATG'y
Cuts at: 1132 1229 2476 2480 2566 2731 4358 5903 1132
Size: 97 1247 4 86 165 1627 1545 2229
Fragments arranged by size:
2229 1627 1545 1247 165 97 86 4
PasI CC'CwG_GG
Cuts at: 1348 2284 3118 1348
Size: 936 834 5230
Fragments arranged by size:
5230 936 834
PciI A'CATG_T
Cuts at: 5899 5899
Size: 7000
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2782 3109 3838 1108
Size: 1674 327 729 4270
Fragments arranged by size:
4270 1674 729 327
PfoI T'CCnGG_A
Cuts at: 2460 2592 3345 4164 2460
Size: 132 753 819 5296
Fragments arranged by size:
5296 819 753 132
PmeI GTTT'AAAC
Cuts at: 4304 4304
Size: 7000
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5069 5101 6582 6614 5069
Size: 32 1481 32 5455
Fragments arranged by size:
5455 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2615 2815 2615
Size: 200 6800
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7000
PsiI TTA'TAA
Cuts at: 4570 4570
Size: 7000
PspOMI G'GGCC_C
Cuts at: 3021 3291 3021
Size: 270 6730
PstI C_TGCA'G
Cuts at: 830 2883 3706 3945 3970 4352 830
Size: 2053 823 239 25 382 3478
Fragments arranged by size:
3478 2053 823 382 239 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7000
PvuII CAG'CTG
Cuts at: 3707 4156 3707
Size: 449 6551
SalI G'TCGA_C
Cuts at: 1936 4342 1936
Size: 2406 4594
SbfI CC_TGCA'GG
Cuts at: 4352 4352
Size: 7000
ScaI AGT'ACT
Cuts at: 5248 6960 5248
Size: 1712 5288
SfcI C'TryA_G
Cuts at: 826 1045 2741 2879 3702 3941 3966 4348 5483
Size: 219 1696 138 823 239 25 382 1135
Cuts at: 5483 6164 6355 826
Size: 681 191 1471
Fragments arranged by size:
1696 1471 1135 823 681 382 239 219
191 138 25
SfoI GGC'GCC
Cuts at: 2081 4150 2081
Size: 2069 4931
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7000
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7000
SmaI CCC'GGG
Cuts at: 1872 2956 4327 1872
Size: 1084 1371 4545
Fragments arranged by size:
4545 1371 1084
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 3761 4106 5104 6005 6267
Size: 197 926 498 1320 345 998 901 262
Cuts at: 6267 820
Size: 1553
Fragments arranged by size:
1553 1320 998 926 901 498 345 262
197
SnaBI TAC'GTA
Cuts at: 493 4791 493
Size: 4298 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7000
SphI G_CATG'C
Cuts at: 1132 2566 2731 4358 1132
Size: 1434 165 1627 3774
Fragments arranged by size:
3774 1627 1434 165
SspI AAT'ATT
Cuts at: 5 52 4924 5
Size: 47 4872 2081
Fragments arranged by size:
4872 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7000
StyI C'CwwG_G
Cuts at: 513 1065 2004 4439 513
Size: 552 939 2435 3074
Fragments arranged by size:
3074 2435 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2354 3788 3851 4256 5027 5044 5197
Size: 1255 774 1434 63 405 771 17 153
Cuts at: 5197 5382 5827 325
Size: 185 445 1498
Fragments arranged by size:
1498 1434 1255 774 771 445 405 185
153 63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2535 2631 5246
Size: 274 80 33 51 1443 558 96 2615
Cuts at: 5246 6958 96
Size: 1712 138
Fragments arranged by size:
2615 1712 1443 558 274 138 96 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6960 6992 6960
Size: 32 6968
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7000
XbaI T'CTAG_A
Cuts at: 2839 4336 2839
Size: 1497 5503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2317 3182 2317
Size: 865 6135
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7000
XmaI C'CCGG_G
Cuts at: 1870 2954 4325 1870
Size: 1084 1371 4545
Fragments arranged by size:
4545 1371 1084
XmnI GAAnn'nnTTC
Cuts at: 5129 5129
Size: 7000
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4162 4808 276
Size: 53 83 186 936 2628 646 2468
Fragments arranged by size:
2628 2468 936 646 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstZ17I
Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI