Restriction map for
pFN21AE3291
(Circular) (Six-base) MAPSORT of: pFN21AE3291.seq Check: 8507 from: 1 to: 6964
pFN21AE3291 6964 bp
With 182 enzymes: SgfI *
March 28, 2012 12:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4143 4774 278
Size: 53 83 186 936 2607 631 2468
Fragments arranged by size:
2607 2468 936 631 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3398 3639 4307 1937
Size: 1461 241 668 4594
Fragments arranged by size:
4594 1461 668 241
Acc65I G'GTAC_C
Cuts at: 4285 4285
Size: 6964
AclI AA'CG_TT
Cuts at: 1563 5091 5464 1563
Size: 3528 373 3063
Fragments arranged by size:
3528 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2423 3128 5027 6411 6679 1632
Size: 791 705 1899 1384 268 1917
Fragments arranged by size:
1917 1899 1384 791 705 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6767
AflIII A'CryG_T
Cuts at: 1203 2938 3056 5863 6840 1203
Size: 1735 118 2807 977 1327
Fragments arranged by size:
2807 1735 1327 977 118
AgeI A'CCGG_T
Cuts at: 5795 5795
Size: 6964
AhdI GACnn_n'nnGTC
Cuts at: 5693 5693
Size: 6964
AleI CACnn'nnGTG
Cuts at: 1166 2321 1166
Size: 1155 5809
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6964
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3180 3212 3180
Size: 32 6932
AlwNI CAG_nnn'CTG
Cuts at: 2599 3003 3476 4707 6279 2599
Size: 404 473 1231 1572 3284
Fragments arranged by size:
3284 1572 1231 473 404
ApaLI G'TGCA_C
Cuts at: 5021 6177 5021
Size: 1156 5808
ApoI r'AATT_y
Cuts at: 1493 3254 3495 3677 4273 4499 4651 6760 1493
Size: 1761 241 182 596 226 152 2109 1697
Fragments arranged by size:
2109 1761 1697 596 241 226 182 152
AseI AT'TA_AT
Cuts at: 160 1032 3435 5518 160
Size: 872 2403 2083 1606
Fragments arranged by size:
2403 2083 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
AvaI C'yCGr_G
Cuts at: 1870 1943 3537 4289 1870
Size: 73 1594 752 4545
Fragments arranged by size:
4545 1594 752 73
BamHI G'GATC_C
Cuts at: 4294 4661 4294
Size: 367 6597
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4285 5740 618
Size: 325 217 627 2498 1455 1842
Fragments arranged by size:
2498 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4283 1391
Size: 2892 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5993
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3715 3749 5155 5189 1903
Size: 34 1778 34 1406 34 3678
Fragments arranged by size:
3678 1778 1406 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4856 6066 4856
Size: 1210 5754
BclI T'GATC_A
Cuts at: 1548 3042 1548
Size: 1494 5470
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5575 136
Size: 107 122 71 631 605 3903 1525
Fragments arranged by size:
3903 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6959 6959
Size: 6964
BlpI GC'TnA_GC
Cuts at: 4381 4381
Size: 6964
Bme1580I G_kGCm'C
Cuts at: 1790 5025 6181 1790
Size: 3235 1156 2573
Fragments arranged by size:
3235 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2941 3059 4177 1318
Size: 1623 118 1118 4105
Fragments arranged by size:
4105 1623 1118 118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3806 4695 5653
Size: 416 53 83 399 24 2389 889 958
Cuts at: 5653 442
Size: 1753
Fragments arranged by size:
2389 1753 958 889 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6964
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3301 3601 3301
Size: 300 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5624 1239
Size: 4385 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3273 5089 5954 6252 3273
Size: 1816 865 298 3985
Fragments arranged by size:
3985 1816 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5627 882
Size: 617 4128 2219
Fragments arranged by size:
4128 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4755 493
Size: 711 3551 2702
Fragments arranged by size:
3551 2702 711
BsaBI GATnn'nnATC
Cuts at: 2477 6958 2477
Size: 4481 2483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4140 4771 5153 5788
Size: 53 83 186 936 2607 631 382 635
Cuts at: 5788 275
Size: 1451
Fragments arranged by size:
2607 1451 936 635 631 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4100 5397 5795 6069 6216 6627 4100
Size: 1297 398 274 147 411 4437
Fragments arranged by size:
4437 1297 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3761 3791 1178
Size: 30 165 30 2358 30 4351
Fragments arranged by size:
4351 2358 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6964
BseYI C'CCAG_C
Cuts at: 4216 6167 6663 4216
Size: 1951 496 4517
Fragments arranged by size:
4517 1951 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2175 2560 2607 2949 3122 4190 1163
Size: 573 439 385 47 342 173 1068 3937
Fragments arranged by size:
3937 1068 573 439 385 342 173 47
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4414 5175 6203 1540
Size: 275 186 2413 761 1028 2301
Fragments arranged by size:
2413 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 5025 5110 6181 5025
Size: 85 1071 5808
Fragments arranged by size:
5808 1071 85
BsmI GAATG_Cn'
Cuts at: 2779 3556 4475 4568 2779
Size: 777 919 93 5175
Fragments arranged by size:
5175 919 777 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4283 5025 5110 6181 1391
Size: 399 2493 742 85 1071 2174
Fragments arranged by size:
2493 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 4100 6627 4100
Size: 2527 4437
BspHI T'CATG_A
Cuts at: 2053 2415 4853 6583 2053
Size: 362 2438 1730 2434
Fragments arranged by size:
2438 2434 1730 362
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3927 4319 844
Size: 3083 392 3489
Fragments arranged by size:
3489 3083 392
BsrBI CCG'CTC
Cuts at: 1394 1823 4851 1394
Size: 429 3028 3507
Fragments arranged by size:
3507 3028 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4006 5459 5633 65
Size: 1183 2758 1453 174 1396
Fragments arranged by size:
2758 1453 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5608 5795 1700
Size: 110 3798 187 2869
Fragments arranged by size:
3798 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6964
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6964
BssSI C'ACGA_G
Cuts at: 5024 6036 5024
Size: 1012 5952
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3136 794
Size: 2342 4622
BstBI TT'CG_AA
Cuts at: 5768 5768
Size: 6964
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6964
BstYI r'GATC_y
Cuts at: 1972 4257 4294 4661 5046 5063 6515 6601 6613
Size: 2285 37 367 385 17 1452 86 12
Cuts at: 6613 6959 1972
Size: 346 1977
Fragments arranged by size:
2285 1977 1452 385 367 346 86 37
17 12
BstZ17I GTA'TAC
Cuts at: 3399 3399
Size: 6964
Bsu36I CC'TnA_GG
Cuts at: 3596 3596
Size: 6964
BtgI C'CryG_G
Cuts at: 513 1065 2004 2346 3769 3847 513
Size: 552 939 342 1423 78 3630
Fragments arranged by size:
3630 1423 939 552 342 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6225
Fragments arranged by size:
6225 683 56
BtsI GCAGTG_nn'
Cuts at: 2590 3134 3242 4480 5274 5294 2590
Size: 544 108 1238 794 20 4260
Fragments arranged by size:
4260 1238 794 544 108 20
ClaI AT'CG_AT
Cuts at: 1554 3228 1554
Size: 1674 5290
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6929
DraI TTT'AAA
Cuts at: 4268 4268
Size: 6964
DraIII CAC_nnn'GTG
Cuts at: 2761 2761
Size: 6964
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5971 809
Size: 507 4655 1802
Fragments arranged by size:
4655 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2454 3327 3523
Size: 54 1425 50 275 18 624 873 196
Cuts at: 3523 3682 4110 4411 5300 6929 8
Size: 159 428 301 889 1629 43
Fragments arranged by size:
1629 1425 889 873 624 428 301 275
196 159 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4411 1537
Size: 275 2599 4090
Fragments arranged by size:
4090 2599 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3064 3420 4894 1353
Size: 1711 356 1474 3423
Fragments arranged by size:
3423 1711 1474 356
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3683 5551 5925 6071 387
Size: 1064 138 2094 1868 374 146 1280
Fragments arranged by size:
2094 1868 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2423 3128 5027 5624 6411 6679 1239
Size: 393 791 705 1899 597 787 268 1524
Fragments arranged by size:
1899 1524 791 787 705 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3540 3886 3540
Size: 346 6618
EcoO109I rG'GnC_Cy
Cuts at: 2048 3600 3916 2048
Size: 1552 316 5096
Fragments arranged by size:
5096 1552 316
EcoRI G'AATT_C
Cuts at: 4273 4651 4273
Size: 378 6586
EcoRV GAT'ATC
Cuts at: 2413 2604 2413
Size: 191 6773
FspI TGC'GCA
Cuts at: 5470 5470
Size: 6964
HaeII r_GCGC'y
Cuts at: 1678 6111 1678
Size: 4433 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2711 2743 5605 5637 5679
Size: 32 135 32 1139 32 2862 32 42
Cuts at: 5679 5711 1373
Size: 32 2626
Fragments arranged by size:
2862 2626 1139 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4204 4308 4554 669
Size: 988 281 2266 104 246 3079
Fragments arranged by size:
3079 2266 988 281 246 104
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6964
HpaI GTT'AAC
Cuts at: 4554 4554
Size: 6964
KpnI G_GTAC'C
Cuts at: 4289 4289
Size: 6964
MfeI C'AATT_G
Cuts at: 2319 4563 2319
Size: 2244 4720
MluI A'CGCG_T
Cuts at: 6840 6840
Size: 6964
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3001 6078 6262 1305
Size: 1696 3077 184 2007
Fragments arranged by size:
3077 2007 1696 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2456 3684 4112 10
Size: 54 1425 343 624 1228 428 2862
Fragments arranged by size:
2862 1425 1228 624 428 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 2565 2702 2789 3756 4922 5281
Size: 648 1155 244 137 87 967 1166 359
Cuts at: 5281 5440 518
Size: 159 2042
Fragments arranged by size:
2042 1166 1155 967 648 359 244 159
137 87
MspA1I CmG'CkG
Cuts at: 1606 3003 4031 4380 5057 6205 6450 1606
Size: 1397 1028 349 677 1148 245 2120
Fragments arranged by size:
2120 1397 1148 1028 677 349 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2346 3847 513
Size: 552 939 342 1501 3630
Fragments arranged by size:
3630 1501 939 552 342
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6964
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6964
NotI GC'GGCC_GC
Cuts at: 4411 4411
Size: 6964
NspI r_CATG'y
Cuts at: 1132 1229 2543 2707 4322 5867 1132
Size: 97 1314 164 1615 1545 2229
Fragments arranged by size:
2229 1615 1545 1314 164 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6964
PciI A'CATG_T
Cuts at: 5863 5863
Size: 6964
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6964
PfoI T'CCnGG_A
Cuts at: 3099 3099
Size: 6964
PmeI GTTT'AAAC
Cuts at: 4268 4268
Size: 6964
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5033 5065 6546 6578 5033
Size: 32 1481 32 5419
Fragments arranged by size:
5419 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2048 3600 3916 2048
Size: 1552 316 5096
Fragments arranged by size:
5096 1552 316
PshAI GACnn'nnGTC
Cuts at: 1613 2840 1613
Size: 1227 5737
PsiI TTA'TAA
Cuts at: 4534 4534
Size: 6964
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2669 2701 3389 3421 2669
Size: 32 688 32 6212
Fragments arranged by size:
6212 688 32 32
PstI C_TGCA'G
Cuts at: 830 2028 3469 3494 3793 4316 830
Size: 1198 1441 25 299 523 3478
Fragments arranged by size:
3478 1441 1198 523 299 25
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6964
PvuII CAG'CTG
Cuts at: 3003 4031 3003
Size: 1028 5936
SalI G'TCGA_C
Cuts at: 1936 4306 1936
Size: 2370 4594
SbfI CC_TGCA'GG
Cuts at: 4316 4316
Size: 6964
ScaI AGT'ACT
Cuts at: 5212 6924 5212
Size: 1712 5252
SfcI C'TryA_G
Cuts at: 826 1045 2024 2750 3446 3465 3490 3789 4312
Size: 219 979 726 696 19 25 299 523
Cuts at: 4312 5447 6128 6319 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 979 726 696 681 523 299
219 191 25 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6964
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6964
SmaI CCC'GGG
Cuts at: 1872 4291 1872
Size: 2419 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3288 5068 5969 6231 820
Size: 197 926 1345 1780 901 262 1553
Fragments arranged by size:
1780 1553 1345 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4755 493
Size: 4262 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6964
SphI G_CATG'C
Cuts at: 1132 4322 1132
Size: 3190 3774
SspI AAT'ATT
Cuts at: 5 52 2857 3126 4888 5
Size: 47 2805 269 1762 2081
Fragments arranged by size:
2805 2081 1762 269 47
StuI AGG'CCT
Cuts at: 1517 2364 4157 1517
Size: 847 1793 4324
Fragments arranged by size:
4324 1793 847
StyI C'CwwG_G
Cuts at: 513 1065 2004 2346 3200 3324 3685 3847 4403
Size: 552 939 342 854 124 361 162 556
Cuts at: 4403 513
Size: 3074
Fragments arranged by size:
3074 939 854 556 552 361 342 162
124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3128 3840 3953 4208 4991 5008 5161
Size: 1255 1548 712 113 255 783 17 153
Cuts at: 5161 5346 5791 325
Size: 185 445 1498
Fragments arranged by size:
1548 1498 1255 783 712 445 255 185
153 113 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5210 6922 96
Size: 274 80 33 51 1443 3233 1712 138
Fragments arranged by size:
3233 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 3024 3056 3761 3793 6924 6956 2588
Size: 32 404 32 705 32 3131 32 2596
Fragments arranged by size:
3131 2596 705 404 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6964
XbaI T'CTAG_A
Cuts at: 4300 4300
Size: 6964
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2551 4234 2551
Size: 1683 5281
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6964
XmaI C'CCGG_G
Cuts at: 1870 4289 1870
Size: 2419 4545
XmnI GAAnn'nnTTC
Cuts at: 5093 5093
Size: 6964
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4141 4772 276
Size: 53 83 186 936 2607 631 2468
Fragments arranged by size:
2607 2468 936 631 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstXI EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI