Restriction map for
pFN21AE3286
(Circular) (Six-base) MAPSORT of: pFN21AE3286.seq Check: 9685 from: 1 to: 6904
pFN21AE3286 6904 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4714 278
Size: 53 83 186 936 3178 2468
Fragments arranged by size:
3178 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2497 4247 1937
Size: 560 1750 4594
Fragments arranged by size:
4594 1750 560
Acc65I G'GTAC_C
Cuts at: 4225 4225
Size: 6904
AclI AA'CG_TT
Cuts at: 1563 5031 5404 1563
Size: 3468 373 3063
Fragments arranged by size:
3468 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3408 4152 4967 6351 6619 1632
Size: 1776 744 815 1384 268 1917
Fragments arranged by size:
1917 1776 1384 815 744 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6707
AflIII A'CryG_T
Cuts at: 1203 2608 5803 6780 1203
Size: 1405 3195 977 1327
Fragments arranged by size:
3195 1405 1327 977
AgeI A'CCGG_T
Cuts at: 5735 5735
Size: 6904
AhdI GACnn_n'nnGTC
Cuts at: 5633 5633
Size: 6904
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6904
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3355 4207 1184
Size: 2171 852 3881
Fragments arranged by size:
3881 2171 852
AlwNI CAG_nnn'CTG
Cuts at: 2174 4130 4145 4647 6219 2174
Size: 1956 15 502 1572 2859
Fragments arranged by size:
2859 1956 1572 502 15
ApaLI G'TGCA_C
Cuts at: 4961 6117 4961
Size: 1156 5748
ApoI r'AATT_y
Cuts at: 1493 2281 2305 2387 2431 2456 2685 2935 3486
Size: 788 24 82 44 25 229 250 551
Cuts at: 3486 3856 4213 4439 4591 6700 1493
Size: 370 357 226 152 2109 1697
Fragments arranged by size:
2109 1697 788 551 370 357 250 229
226 152 82 44 25 24
AseI AT'TA_AT
Cuts at: 160 1032 3692 5458 160
Size: 872 2660 1766 1606
Fragments arranged by size:
2660 1766 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6904
AvaI C'yCGr_G
Cuts at: 1870 1943 2088 4229 1870
Size: 73 145 2141 4545
Fragments arranged by size:
4545 2141 145 73
BamHI G'GATC_C
Cuts at: 4234 4601 4234
Size: 367 6537
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2616 4225 5680 618
Size: 325 217 627 829 1609 1455 1842
Fragments arranged by size:
1842 1609 1455 829 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2551 4223 1391
Size: 1160 1672 4072
Fragments arranged by size:
4072 1672 1160
BbeI G_GCGC'C
Cuts at: 2620 2620
Size: 6904
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5933
BbvCI CC'TCA_GC
Cuts at: 2817 2817
Size: 6904
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5095 5129 1903
Size: 34 3158 34 3678
Fragments arranged by size:
3678 3158 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4796 6006 4796
Size: 1210 5694
BclI T'GATC_A
Cuts at: 1548 3849 1548
Size: 2301 4603
BfrBI ATG'CAT
Cuts at: 3091 3740 3091
Size: 649 6255
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5515 136
Size: 107 122 71 631 605 3843 1525
Fragments arranged by size:
3843 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3098 6899 3098
Size: 3801 3103
BlpI GC'TnA_GC
Cuts at: 4321 4321
Size: 6904
Bme1580I G_kGCm'C
Cuts at: 1790 2378 4965 6121 1790
Size: 588 2587 1156 2573
Fragments arranged by size:
2587 2573 1156 588
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6904
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2871 4635 5593
Size: 416 53 83 399 24 1454 1764 958
Cuts at: 5593 442
Size: 1753
Fragments arranged by size:
1764 1753 1454 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6904
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2060 2145 2411 4116 5564 1239
Size: 821 85 266 1705 1448 2579
Fragments arranged by size:
2579 1705 1448 821 266 85
Bpu10I CC'TnA_GC
Cuts at: 2817 2817
Size: 6904
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3966 5029 5894 6192 3966
Size: 1063 865 298 4678
Fragments arranged by size:
4678 1063 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5567 882
Size: 617 4068 2219
Fragments arranged by size:
4068 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4695 493
Size: 711 3491 2702
Fragments arranged by size:
3491 2702 711
BsaBI GATnn'nnATC
Cuts at: 2477 6898 2477
Size: 4421 2483
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2617 3247 4711 5093
Size: 53 83 186 936 1084 630 1464 382
Cuts at: 5093 5728 275
Size: 635 1451
Fragments arranged by size:
1464 1451 1084 936 635 630 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5337 5735 6009 6156 6567 5337
Size: 398 274 147 411 5674
Fragments arranged by size:
5674 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6679
Fragments arranged by size:
6679 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2036 2039 2063 2240 1186
Size: 850 3 24 177 5850
Fragments arranged by size:
5850 850 177 24 3
BseYI C'CCAG_C
Cuts at: 2099 2107 2167 6107 6603 2099
Size: 8 60 3940 496 2400
Fragments arranged by size:
3940 2400 496 60 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6331
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4354 5115 6143 1540
Size: 275 186 2353 761 1028 2301
Fragments arranged by size:
2353 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4965 5050 6121 4965
Size: 85 1071 5748
Fragments arranged by size:
5748 1071 85
BsmI GAATG_Cn'
Cuts at: 3158 3639 4415 4508 3158
Size: 481 776 93 5554
Fragments arranged by size:
5554 776 481 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2378 2551 4223 4965 5050 6121 1391
Size: 399 588 173 1672 742 85 1071 2174
Fragments arranged by size:
2174 1672 1071 742 588 399 173 85
BspEI T'CCGG_A
Cuts at: 6567 6567
Size: 6904
BspHI T'CATG_A
Cuts at: 4793 6523 4793
Size: 1730 5174
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4259 844
Size: 3415 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4791 1394
Size: 429 2968 3507
Fragments arranged by size:
3507 2968 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3816 5399 5573 65
Size: 1183 2568 1583 174 1396
Fragments arranged by size:
2568 1583 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5548 5735 1700
Size: 110 3738 187 2869
Fragments arranged by size:
3738 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2710 2798 96
Size: 2614 88 4202
Fragments arranged by size:
4202 2614 88
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6904
BssSI C'ACGA_G
Cuts at: 4964 5976 4964
Size: 1012 5892
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3329 794
Size: 2535 4369
BstBI TT'CG_AA
Cuts at: 5708 5708
Size: 6904
BstEII G'GTnAC_C
Cuts at: 1184 3061 1184
Size: 1877 5027
BstXI CCAn_nnnn'nTGG
Cuts at: 2542 2542
Size: 6904
BstYI r'GATC_y
Cuts at: 1972 3098 4234 4601 4986 5003 6455 6541 6553
Size: 1126 1136 367 385 17 1452 86 12
Cuts at: 6553 6899 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1136 1126 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2223 2379 2679 2223
Size: 156 300 6448
Fragments arranged by size:
6448 300 156
BtgI C'CryG_G
Cuts at: 513 1065 2004 3655 513
Size: 552 939 1651 3762
Fragments arranged by size:
3762 1651 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6165
Fragments arranged by size:
6165 683 56
BtsI GCAGTG_nn'
Cuts at: 2664 3324 3375 4420 5214 5234 2664
Size: 660 51 1045 794 20 4334
Fragments arranged by size:
4334 1045 794 660 51 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6904
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6869
DraI TTT'AAA
Cuts at: 2970 4057 4071 4208 2970
Size: 1087 14 137 5666
Fragments arranged by size:
5666 1087 137 14
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2364 5911 809
Size: 507 1048 3547 1802
Fragments arranged by size:
3547 1802 1048 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4351 5240 6869
Size: 54 1425 50 275 18 2521 889 1629
Cuts at: 6869 8
Size: 43
Fragments arranged by size:
2521 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4351 1537
Size: 275 2539 4090
Fragments arranged by size:
4090 2539 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3474 3654 4834 1353
Size: 2121 180 1180 3423
Fragments arranged by size:
3423 2121 1180 180
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2033 5491 5865 6011 387
Size: 1064 138 444 3458 374 146 1280
Fragments arranged by size:
3458 1280 1064 444 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2060 2145 2411 3408 4116 4152 4967
Size: 393 428 85 266 997 708 36 815
Cuts at: 4967 5564 6351 6619 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 997 815 787 708 597 428 393
268 266 85 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2042 2183 2042
Size: 141 6763
EcoO109I rG'GnC_Cy
Cuts at: 2074 2104 3416 2074
Size: 30 1312 5562
Fragments arranged by size:
5562 1312 30
EcoRI G'AATT_C
Cuts at: 4213 4591 4213
Size: 378 6526
EcoRV GAT'ATC
Cuts at: 3208 3208
Size: 6904
FspI TGC'GCA
Cuts at: 5410 5410
Size: 6904
HaeII r_GCGC'y
Cuts at: 1678 2620 6051 1678
Size: 942 3431 2531
Fragments arranged by size:
3431 2531 942
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2627 2659 3451 3483 5545
Size: 32 135 32 1055 32 792 32 2062
Cuts at: 5545 5577 5619 5651 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2062 1055 792 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2498 3754 4248 4494 669
Size: 988 281 560 1256 494 246 3079
Fragments arranged by size:
3079 1256 988 560 494 281 246
HindIII A'AGCT_T
Cuts at: 748 2411 748
Size: 1663 5241
HpaI GTT'AAC
Cuts at: 4494 4494
Size: 6904
KasI G'GCGC_C
Cuts at: 2616 2616
Size: 6904
KpnI G_GTAC'C
Cuts at: 4229 4229
Size: 6904
MfeI C'AATT_G
Cuts at: 4503 4503
Size: 6904
MluI A'CGCG_T
Cuts at: 6780 6780
Size: 6904
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2168 6018 6202 1305
Size: 863 3850 184 2007
Fragments arranged by size:
3850 2007 863 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5082
Fragments arranged by size:
5082 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4862 5221 5380 518
Size: 648 3696 359 159 2042
Fragments arranged by size:
3696 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3535 4320 4997 6145 6390 1606
Size: 1929 785 677 1148 245 2120
Fragments arranged by size:
2120 1929 1148 785 677 245
NarI GG'CG_CC
Cuts at: 2617 2617
Size: 6904
NcoI C'CATG_G
Cuts at: 513 1065 2004 3655 513
Size: 552 939 1651 3762
Fragments arranged by size:
3762 1651 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6904
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6904
NotI GC'GGCC_GC
Cuts at: 4351 4351
Size: 6904
NsiI A_TGCA'T
Cuts at: 3093 3742 3093
Size: 649 6255
NspI r_CATG'y
Cuts at: 1132 1229 2612 3239 3562 4262 5807 1132
Size: 97 1383 627 323 700 1545 2229
Fragments arranged by size:
2229 1545 1383 700 627 323 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6904
PciI A'CATG_T
Cuts at: 2608 5803 2608
Size: 3195 3709
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3661 1108
Size: 2553 4351
PmeI GTTT'AAAC
Cuts at: 4208 4208
Size: 6904
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4973 5005 6486 6518 4973
Size: 32 1481 32 5359
Fragments arranged by size:
5359 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2074 2104 3416 2074
Size: 30 1312 5562
Fragments arranged by size:
5562 1312 30
PshAI GACnn'nnGTC
Cuts at: 1613 3881 1613
Size: 2268 4636
PsiI TTA'TAA
Cuts at: 4474 4474
Size: 6904
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2554 2586 2554
Size: 32 6872
PstI C_TGCA'G
Cuts at: 830 3319 3334 4196 4256 830
Size: 2489 15 862 60 3478
Fragments arranged by size:
3478 2489 862 60 15
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6904
PvuII CAG'CTG
Cuts at: 3535 3535
Size: 6904
SalI G'TCGA_C
Cuts at: 1936 2496 4246 1936
Size: 560 1750 4594
Fragments arranged by size:
4594 1750 560
SanDI GG'GwC_CC
Cuts at: 2104 2104
Size: 6904
SbfI CC_TGCA'GG
Cuts at: 4256 4256
Size: 6904
ScaI AGT'ACT
Cuts at: 3360 3889 5152 6864 3360
Size: 529 1263 1712 3400
Fragments arranged by size:
3400 1712 1263 529
SfcI C'TryA_G
Cuts at: 826 1045 3201 3315 3330 3789 4192 4252 5387
Size: 219 2156 114 15 459 403 60 1135
Cuts at: 5387 6068 6259 826
Size: 681 191 1471
Fragments arranged by size:
2156 1471 1135 681 459 403 219 191
114 60 15
SfoI GGC'GCC
Cuts at: 2618 2618
Size: 6904
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6904
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6904
SmaI CCC'GGG
Cuts at: 1872 2090 4231 1872
Size: 218 2141 4545
Fragments arranged by size:
4545 2141 218
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3981 5008 5909 6171 820
Size: 197 926 2038 1027 901 262 1553
Fragments arranged by size:
2038 1553 1027 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4695 493
Size: 4202 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6904
SphI G_CATG'C
Cuts at: 1132 3562 4262 1132
Size: 2430 700 3774
Fragments arranged by size:
3774 2430 700
SspI AAT'ATT
Cuts at: 5 52 2793 3055 3281 4828 5
Size: 47 2741 262 226 1547 2081
Fragments arranged by size:
2741 2081 1547 262 226 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6904
StyI C'CwwG_G
Cuts at: 513 1065 2004 3178 3225 3655 4343 513
Size: 552 939 1174 47 430 688 3074
Fragments arranged by size:
3074 1174 939 688 552 430 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4013 4931 4948 5101 5286 5731 325
Size: 1255 2433 918 17 153 185 445 1498
Fragments arranged by size:
2433 1498 1255 918 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2710 2798 3082
Size: 274 80 33 51 1443 733 88 284
Cuts at: 3082 3358 3887 4074 5150 6862 96
Size: 276 529 187 1076 1712 138
Fragments arranged by size:
1712 1443 1076 733 529 284 276 274
187 138 88 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3123 3155 6864 6896 3123
Size: 32 3709 32 3131
Fragments arranged by size:
3709 3131 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6904
XbaI T'CTAG_A
Cuts at: 2275 2842 3118 3148 4240 2275
Size: 567 276 30 1092 4939
Fragments arranged by size:
4939 1092 567 276 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2866 2011
Size: 855 6049
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6904
XmaI C'CCGG_G
Cuts at: 1870 2088 4229 1870
Size: 218 2141 4545
Fragments arranged by size:
4545 2141 218
XmnI GAAnn'nnTTC
Cuts at: 3648 5033 3648
Size: 1385 5519
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4712 276
Size: 53 83 186 936 3178 2468
Fragments arranged by size:
3178 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI
PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI
RsrII SacI SacII SapI SexAI SfiI SrfI SwaI