Restriction map for
pFN21AE3281
(Circular) (Six-base) MAPSORT of: pFN21AE3281.seq Check: 7620 from: 1 to: 6841
pFN21AE3281 6841 bp
With 182 enzymes: SgfI *
August 20, 2012 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2851 2851
Size: 6841
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4651 278
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4184 1937
Size: 2247 4594
Acc65I G'GTAC_C
Cuts at: 4162 4162
Size: 6841
AclI AA'CG_TT
Cuts at: 1563 4968 5341 1563
Size: 3405 373 3063
Fragments arranged by size:
3405 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3328 3512 3906 4904 6288 6556 1632
Size: 1696 184 394 998 1384 268 1917
Fragments arranged by size:
1917 1696 1384 998 394 268 184
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6644
AflIII A'CryG_T
Cuts at: 1203 5740 6717 1203
Size: 4537 977 1327
Fragments arranged by size:
4537 1327 977
AgeI A'CCGG_T
Cuts at: 2884 5672 2884
Size: 2788 4053
AhdI GACnn_n'nnGTC
Cuts at: 5570 5570
Size: 6841
AleI CACnn'nnGTG
Cuts at: 1166 4067 1166
Size: 2901 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3303 1184
Size: 2119 4722
AlwNI CAG_nnn'CTG
Cuts at: 2246 4584 6156 2246
Size: 2338 1572 2931
Fragments arranged by size:
2931 2338 1572
ApaLI G'TGCA_C
Cuts at: 4898 6054 4898
Size: 1156 5685
ApoI r'AATT_y
Cuts at: 1493 2260 2290 2650 2985 3040 3469 4150 4376
Size: 767 30 360 335 55 429 681 226
Cuts at: 4376 4528 6637 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 767 681 429 360 335 226
152 55 30
AseI AT'TA_AT
Cuts at: 160 1032 3263 3522 5395 160
Size: 872 2231 259 1873 1606
Fragments arranged by size:
2231 1873 1606 872 259
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
AvaI C'yCGr_G
Cuts at: 1870 1943 2217 2664 4166 1870
Size: 73 274 447 1502 4545
Fragments arranged by size:
4545 1502 447 274 73
AvrII C'CTAG_G
Cuts at: 3134 3134
Size: 6841
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2661 2694 2661
Size: 33 6808
BamHI G'GATC_C
Cuts at: 2437 4171 4538 2437
Size: 1734 367 4740
Fragments arranged by size:
4740 1734 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2103 2660 4162 5617 618
Size: 325 217 627 316 557 1502 1455 1842
Fragments arranged by size:
1842 1502 1455 627 557 325 316 217
BanII G_rGCy'C
Cuts at: 1391 2190 2218 2386 2635 2847 3134 3835 4160
Size: 799 28 168 249 212 287 701 325
Cuts at: 4160 1391
Size: 4072
Fragments arranged by size:
4072 799 701 325 287 249 212 168
28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 3129 928
Size: 971 137 1093 4640
Fragments arranged by size:
4640 1093 971 137
BbvCI CC'TCA_GC
Cuts at: 2307 2307
Size: 6841
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 5032 5066 1903
Size: 34 50 34 3011 34 3678
Fragments arranged by size:
3678 3011 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2874 3469 4733 5943 2874
Size: 595 1264 1210 3772
Fragments arranged by size:
3772 1264 1210 595
BclI T'GATC_A
Cuts at: 1548 2011 3052 1548
Size: 463 1041 5337
Fragments arranged by size:
5337 1041 463
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2198 5452 136
Size: 107 122 71 631 605 526 3254 1525
Fragments arranged by size:
3254 1525 631 605 526 122 107 71
BglII A'GATC_T
Cuts at: 2086 2328 3402 3967 6836 2086
Size: 242 1074 565 2869 2091
Fragments arranged by size:
2869 2091 1074 565 242
BlpI GC'TnA_GC
Cuts at: 3922 4258 3922
Size: 336 6505
Bme1580I G_kGCm'C
Cuts at: 1790 2164 2665 4902 6058 1790
Size: 374 501 2237 1156 2573
Fragments arranged by size:
2573 2237 1156 501 374
BmgBI CAC'GTC
Cuts at: 1318 4065 1318
Size: 2747 4094
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4572 5530 442
Size: 416 53 83 399 24 3155 958 1753
Fragments arranged by size:
3155 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2950 5501 1239
Size: 1711 2551 2579
Fragments arranged by size:
2579 2551 1711
Bpu10I CC'TnA_GC
Cuts at: 2307 2307
Size: 6841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3148 3352 4966 5831 6129 3148
Size: 204 1614 865 298 3860
Fragments arranged by size:
3860 1614 865 298 204
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2966 5504 882
Size: 617 1467 2538 2219
Fragments arranged by size:
2538 2219 1467 617
BsaAI yAC'GTr
Cuts at: 493 1204 3431 4632 493
Size: 711 2227 1201 2702
Fragments arranged by size:
2702 2227 1201 711
BsaBI GATnn'nnATC
Cuts at: 6835 6835
Size: 6841
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4648 5030 5665 275
Size: 53 83 186 936 3115 382 635 1451
Fragments arranged by size:
3115 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2884 5274 5672 5946 6093 6504 2884
Size: 2390 398 274 147 411 3221
Fragments arranged by size:
3221 2390 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2874 2904 1178
Size: 30 165 30 1471 30 5115
Fragments arranged by size:
5115 1471 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2882 2915 3049 1186
Size: 1696 33 134 4978
Fragments arranged by size:
4978 1696 134 33
BseYI C'CCAG_C
Cuts at: 3667 6044 6540 3667
Size: 2377 496 3968
Fragments arranged by size:
3968 2377 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2859 4118 1163
Size: 573 1123 1259 3886
Fragments arranged by size:
3886 1259 1123 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4291 5052 6080 1540
Size: 275 186 2290 761 1028 2301
Fragments arranged by size:
2301 2290 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2218 3204 4902 4987 6058 2218
Size: 986 1698 85 1071 3001
Fragments arranged by size:
3001 1698 1071 986 85
BsmI GAATG_Cn'
Cuts at: 3292 4352 4445 3292
Size: 1060 93 5688
Fragments arranged by size:
5688 1060 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2164 2190 2218 2386 2635 2665 2847
Size: 399 374 26 28 168 249 30 182
Cuts at: 2847 3134 3204 3835 4160 4902 4987 6058 1391
Size: 287 70 631 325 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 631 399 374 325 287
249 182 168 85 70 30 28 26
BspEI T'CCGG_A
Cuts at: 6504 6504
Size: 6841
BspHI T'CATG_A
Cuts at: 4730 6460 4730
Size: 1730 5111
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2851 4196 844
Size: 2007 1345 3489
Fragments arranged by size:
3489 2007 1345
BsrBI CCG'CTC
Cuts at: 1394 1823 4728 1394
Size: 429 2905 3507
Fragments arranged by size:
3507 2905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3648 5336 5510 65
Size: 1183 2400 1688 174 1396
Fragments arranged by size:
2400 1688 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2884 5485 5672 1700
Size: 110 1074 2601 187 2869
Fragments arranged by size:
2869 2601 1074 187 110
BsrGI T'GTAC_A
Cuts at: 96 3804 96
Size: 3708 3133
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6841
BssSI C'ACGA_G
Cuts at: 4901 5913 4901
Size: 1012 5829
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3089 794
Size: 2295 4546
BstBI TT'CG_AA
Cuts at: 5645 5645
Size: 6841
BstEII G'GTnAC_C
Cuts at: 1184 2617 1184
Size: 1433 5408
BstYI r'GATC_y
Cuts at: 1972 2086 2328 2437 3402 3967 4171 4538 4923
Size: 114 242 109 965 565 204 367 385
Cuts at: 4923 4940 6392 6478 6490 6836 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 965 565 385 367 346 242
204 114 109 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6289
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6102
Fragments arranged by size:
6102 683 56
BtsI GCAGTG_nn'
Cuts at: 4357 5151 5171 4357
Size: 794 20 6027
Fragments arranged by size:
6027 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6806
DraI TTT'AAA
Cuts at: 3373 4145 3373
Size: 772 6069
DraIII CAC_nnn'GTG
Cuts at: 2548 3226 2548
Size: 678 6163
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2558 5848 809
Size: 507 1242 3290 1802
Fragments arranged by size:
3290 1802 1242 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4134 4288 5177
Size: 54 1425 50 275 18 2304 154 889
Cuts at: 5177 6806 8
Size: 1629 43
Fragments arranged by size:
2304 1629 1425 889 275 154 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4288 1537
Size: 275 2476 4090
Fragments arranged by size:
4090 2476 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2529 3347 3933 3944 4771 1353
Size: 1176 818 586 11 827 3423
Fragments arranged by size:
3423 1176 827 818 586 11
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5428 5802 5948 387
Size: 1064 138 3839 374 146 1280
Fragments arranged by size:
3839 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2216 2216
Size: 6841
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2950 3328 3512 3906 4904 5501 6288
Size: 393 1318 378 184 394 998 597 787
Cuts at: 6288 6556 1239
Size: 268 1524
Fragments arranged by size:
1524 1318 998 787 597 394 393 378
268 184
EcoO109I rG'GnC_Cy
Cuts at: 2965 2965
Size: 6841
EcoRI G'AATT_C
Cuts at: 4150 4528 4150
Size: 378 6463
EcoRV GAT'ATC
Cuts at: 3436 3436
Size: 6841
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3606 3764 3606
Size: 158 6683
FspI TGC'GCA
Cuts at: 5347 5347
Size: 6841
HaeII r_GCGC'y
Cuts at: 1678 5988 1678
Size: 4310 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2601 2633 3044
Size: 32 135 32 825 32 172 32 411
Cuts at: 3044 3076 5482 5514 5556 5588 1373
Size: 32 2406 32 42 32 2626
Fragments arranged by size:
2626 2406 825 411 172 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2563 4185 4431 669
Size: 988 281 625 1622 246 3079
Fragments arranged by size:
3079 1622 988 625 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6841
HpaI GTT'AAC
Cuts at: 4431 4431
Size: 6841
KpnI G_GTAC'C
Cuts at: 4166 4166
Size: 6841
MfeI C'AATT_G
Cuts at: 4440 4440
Size: 6841
MluI A'CGCG_T
Cuts at: 6717 6717
Size: 6841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4054 5955 6139 1305
Size: 2749 1901 184 2007
Fragments arranged by size:
2749 2007 1901 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4136 10
Size: 54 1425 343 2304 2715
Fragments arranged by size:
2715 2304 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2787 3812 4067 4799 5158 5317 518
Size: 648 1621 1025 255 732 359 159 2042
Fragments arranged by size:
2042 1621 1025 732 648 359 255 159
MspA1I CmG'CkG
Cuts at: 1606 2816 3916 4257 4934 6082 6327 1606
Size: 1210 1100 341 677 1148 245 2120
Fragments arranged by size:
2120 1210 1148 1100 677 341 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6289
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6841
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6841
NotI GC'GGCC_GC
Cuts at: 4288 4288
Size: 6841
NspI r_CATG'y
Cuts at: 1132 1229 4199 5744 1132
Size: 97 2970 1545 2229
Fragments arranged by size:
2970 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6841
PciI A'CATG_T
Cuts at: 5740 5740
Size: 6841
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2467 3226 3674 1108
Size: 1359 759 448 4275
Fragments arranged by size:
4275 1359 759 448
PmeI GTTT'AAAC
Cuts at: 4145 4145
Size: 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4910 4942 6423 6455 4910
Size: 32 1481 32 5296
Fragments arranged by size:
5296 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2965 2965
Size: 6841
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6841
PsiI TTA'TAA
Cuts at: 4411 4411
Size: 6841
PstI C_TGCA'G
Cuts at: 830 2596 3094 4193 830
Size: 1766 498 1099 3478
Fragments arranged by size:
3478 1766 1099 498
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6841
PvuII CAG'CTG
Cuts at: 3916 3916
Size: 6841
SacI G_AGCT'C
Cuts at: 2218 2218
Size: 6841
SalI G'TCGA_C
Cuts at: 1936 4183 1936
Size: 2247 4594
SapI GCTCTTCn'nnn_
Cuts at: 2529 3933 2529
Size: 1404 5437
SbfI CC_TGCA'GG
Cuts at: 4193 4193
Size: 6841
ScaI AGT'ACT
Cuts at: 5089 6801 5089
Size: 1712 5129
SexAI A'CCwGG_T
Cuts at: 3222 3222
Size: 6841
SfcI C'TryA_G
Cuts at: 826 1045 2592 3090 4189 5324 6005 6196 826
Size: 219 1547 498 1099 1135 681 191 1471
Fragments arranged by size:
1547 1471 1135 1099 681 498 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6841
SmaI CCC'GGG
Cuts at: 1872 4168 1872
Size: 2296 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3127 3331 4945 5846 6108 820
Size: 197 926 1184 204 1614 901 262 1553
Fragments arranged by size:
1614 1553 1184 926 901 262 204 197
SnaBI TAC'GTA
Cuts at: 493 4632 493
Size: 4139 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6841
SphI G_CATG'C
Cuts at: 1132 4199 1132
Size: 3067 3774
SspI AAT'ATT
Cuts at: 5 52 3511 3715 3906 4765 5
Size: 47 3459 204 191 859 2081
Fragments arranged by size:
3459 2081 859 204 191 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6841
StyI C'CwwG_G
Cuts at: 513 1065 2340 3134 4280 513
Size: 552 1275 794 1146 3074
Fragments arranged by size:
3074 1275 1146 794 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2459 4868 4885 5038 5223 5668 325
Size: 1255 879 2409 17 153 185 445 1498
Fragments arranged by size:
2409 1498 1255 879 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3804 5087 6799
Size: 274 80 33 51 1443 1827 1283 1712
Cuts at: 6799 96
Size: 138
Fragments arranged by size:
1827 1712 1443 1283 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6801 6833 6801
Size: 32 6809
Tth111I GACn'n_nGTC
Cuts at: 1316 2179 1316
Size: 863 5978
XbaI T'CTAG_A
Cuts at: 4177 4177
Size: 6841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2468 2468
Size: 6841
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6841
XmaI C'CCGG_G
Cuts at: 1870 4166 1870
Size: 2296 4545
XmnI GAAnn'nnTTC
Cuts at: 4970 4970
Size: 6841
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4649 276
Size: 53 83 186 936 3115 2468
Fragments arranged by size:
3115 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BbeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI