Restriction map for
pFN21AE3278
(Circular) (Six-base) MAPSORT of: pFN21AE3278.seq Check: 7395 from: 1 to: 6793
pFN21AE3278 6793 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4603 278
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3660 4136 1937
Size: 1723 476 4594
Fragments arranged by size:
4594 1723 476
Acc65I G'GTAC_C
Cuts at: 4114 4114
Size: 6793
AclI AA'CG_TT
Cuts at: 1563 4920 5293 1563
Size: 3357 373 3063
Fragments arranged by size:
3357 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2249 2261 2392 4856 6240 6508 1632
Size: 617 12 131 2464 1384 268 1917
Fragments arranged by size:
2464 1917 1384 617 268 131 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6596
AflIII A'CryG_T
Cuts at: 1203 2368 2659 2868 5692 6669 1203
Size: 1165 291 209 2824 977 1327
Fragments arranged by size:
2824 1327 1165 977 291 209
AgeI A'CCGG_T
Cuts at: 5624 5624
Size: 6793
AhdI GACnn_n'nnGTC
Cuts at: 5522 5522
Size: 6793
AleI CACnn'nnGTG
Cuts at: 1166 3038 3527 1166
Size: 1872 489 4432
Fragments arranged by size:
4432 1872 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4025 1184
Size: 2841 3952
AlwNI CAG_nnn'CTG
Cuts at: 2455 3394 3570 4536 6108 2455
Size: 939 176 966 1572 3140
Fragments arranged by size:
3140 1572 966 939 176
ApaLI G'TGCA_C
Cuts at: 4850 6006 4850
Size: 1156 5637
ApoI r'AATT_y
Cuts at: 1493 2458 2843 3318 3869 4102 4328 4480 6589
Size: 965 385 475 551 233 226 152 2109
Cuts at: 6589 1493
Size: 1697
Fragments arranged by size:
2109 1697 965 551 475 385 233 226
152
AseI AT'TA_AT
Cuts at: 160 1032 5347 160
Size: 872 4315 1606
Fragments arranged by size:
4315 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
AvaI C'yCGr_G
Cuts at: 1870 1943 2042 2972 4118 1870
Size: 73 99 930 1146 4545
Fragments arranged by size:
4545 1146 930 99 73
BamHI G'GATC_C
Cuts at: 4123 4490 4123
Size: 367 6426
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2110 2985 4114 5569 618
Size: 325 217 627 323 875 1129 1455 1842
Fragments arranged by size:
1842 1455 1129 875 627 325 323 217
BanII G_rGCy'C
Cuts at: 1391 3267 4112 1391
Size: 1876 845 4072
Fragments arranged by size:
4072 1876 845
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2338 4074 928
Size: 971 439 1736 3647
Fragments arranged by size:
3647 1736 971 439
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3605 3639 4984 5018 1903
Size: 34 1668 34 1345 34 3678
Fragments arranged by size:
3678 1668 1345 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3054 4685 5895 3054
Size: 1631 1210 3952
Fragments arranged by size:
3952 1631 1210
BclI T'GATC_A
Cuts at: 1548 3916 1548
Size: 2368 4425
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5404 136
Size: 107 122 71 631 605 3732 1525
Fragments arranged by size:
3732 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6788 6788
Size: 6793
BlpI GC'TnA_GC
Cuts at: 2244 2682 4210 2244
Size: 438 1528 4827
Fragments arranged by size:
4827 1528 438
Bme1580I G_kGCm'C
Cuts at: 1790 4854 6010 1790
Size: 3064 1156 2573
Fragments arranged by size:
3064 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6793
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2867 3613 4524
Size: 416 53 83 399 24 1450 746 911
Cuts at: 4524 5482 442
Size: 958 1753
Fragments arranged by size:
1753 1450 958 911 746 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6793
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2041 2041
Size: 6793
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2910 3648 3860 5453 1239
Size: 1671 738 212 1593 2579
Fragments arranged by size:
2579 1671 1593 738 212
Bpu10I CC'TnA_GC
Cuts at: 2100 2990 2100
Size: 890 5903
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3578 3821 4918 5783 6081 3578
Size: 243 1097 865 298 4290
Fragments arranged by size:
4290 1097 865 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3614 5456 882
Size: 617 2115 1842 2219
Fragments arranged by size:
2219 2115 1842 617
BsaAI yAC'GTr
Cuts at: 493 1204 2542 2660 2871 4584 493
Size: 711 1338 118 211 1713 2702
Fragments arranged by size:
2702 1713 1338 711 211 118
BsaBI GATnn'nnATC
Cuts at: 2813 6787 2813
Size: 3974 2819
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4600 4982 5617 275
Size: 53 83 186 936 3067 382 635 1451
Fragments arranged by size:
3067 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5226 5624 5898 6045 6456 5226
Size: 398 274 147 411 5563
Fragments arranged by size:
5563 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6568
Fragments arranged by size:
6568 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6793
BseYI C'CCAG_C
Cuts at: 5996 6492 5996
Size: 496 6297
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2673 1163
Size: 573 937 5283
Fragments arranged by size:
5283 937 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4243 5004 6032 1540
Size: 275 186 2242 761 1028 2301
Fragments arranged by size:
2301 2242 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2022 3267 4854 4939 6010 2022
Size: 1245 1587 85 1071 2805
Fragments arranged by size:
2805 1587 1245 1071 85
BsmI GAATG_Cn'
Cuts at: 3363 3628 4304 4397 3363
Size: 265 676 93 5759
Fragments arranged by size:
5759 676 265 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 3267 4112 4854 4939 6010 1391
Size: 399 232 1245 845 742 85 1071 2174
Fragments arranged by size:
2174 1245 1071 845 742 399 232 85
BspEI T'CCGG_A
Cuts at: 6456 6456
Size: 6793
BspHI T'CATG_A
Cuts at: 4682 6412 4682
Size: 1730 5063
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 4148 844
Size: 1397 1907 3489
Fragments arranged by size:
3489 1907 1397
BsrBI CCG'CTC
Cuts at: 1394 1823 4680 1394
Size: 429 2857 3507
Fragments arranged by size:
3507 2857 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3777 5288 5462 65
Size: 1183 2529 1511 174 1396
Fragments arranged by size:
2529 1511 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4014 5437 5624 1700
Size: 110 2204 1423 187 2869
Fragments arranged by size:
2869 2204 1423 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6793
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6793
BssSI C'ACGA_G
Cuts at: 2514 4044 4853 5865 2514
Size: 1530 809 1012 3442
Fragments arranged by size:
3442 1530 1012 809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6793
BstBI TT'CG_AA
Cuts at: 2473 5597 2473
Size: 3124 3669
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6793
BstXI CCAn_nnnn'nTGG
Cuts at: 3953 3953
Size: 6793
BstYI r'GATC_y
Cuts at: 1972 2499 3589 4123 4490 4875 4892 6344 6430
Size: 527 1090 534 367 385 17 1452 86
Cuts at: 6430 6442 6788 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1090 534 527 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3661 3661
Size: 6793
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6241
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6054
Fragments arranged by size:
6054 683 56
BtsI GCAGTG_nn'
Cuts at: 4309 5103 5123 4309
Size: 794 20 5979
Fragments arranged by size:
5979 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6793
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6758
DraI TTT'AAA
Cuts at: 2776 4097 2776
Size: 1321 5472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5800 809
Size: 507 4484 1802
Fragments arranged by size:
4484 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4240 5129 6758
Size: 54 1425 50 275 18 2410 889 1629
Cuts at: 6758 8
Size: 43
Fragments arranged by size:
2410 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4240 1537
Size: 275 2428 4090
Fragments arranged by size:
4090 2428 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2163 3617 3645 4723 1353
Size: 810 1454 28 1078 3423
Fragments arranged by size:
3423 1454 1078 810 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3209 3824 5380 5754 5900 387
Size: 1064 138 1620 615 1556 374 146 1280
Fragments arranged by size:
1620 1556 1280 1064 615 374 146 138
EcoICRI GAG'CTC
Cuts at: 3265 3265
Size: 6793
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2249 2261 2392 2910 3648 3860 4856
Size: 393 617 12 131 518 738 212 996
Cuts at: 4856 5453 6240 6508 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 996 787 738 617 597 518 393
268 212 131 12
EcoO109I rG'GnC_Cy
Cuts at: 3286 3420 3286
Size: 134 6659
EcoRI G'AATT_C
Cuts at: 2843 3869 4102 4480 2843
Size: 1026 233 378 5156
Fragments arranged by size:
5156 1026 378 233
EcoRV GAT'ATC
Cuts at: 3404 3404
Size: 6793
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2362 3190 2362
Size: 828 5965
FspI TGC'GCA
Cuts at: 5299 5299
Size: 6793
HaeII r_GCGC'y
Cuts at: 1678 5940 1678
Size: 4262 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2264 2296 5434 5466 5508
Size: 32 135 32 692 32 3138 32 42
Cuts at: 5508 5540 1373
Size: 32 2626
Fragments arranged by size:
3138 2626 692 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4137 4383 669
Size: 988 281 2199 246 3079
Fragments arranged by size:
3079 2199 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6793
HpaI GTT'AAC
Cuts at: 4383 4383
Size: 6793
KpnI G_GTAC'C
Cuts at: 4118 4118
Size: 6793
MfeI C'AATT_G
Cuts at: 4392 4392
Size: 6793
MluI A'CGCG_T
Cuts at: 6669 6669
Size: 6793
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2398 2858 5907 6091 1305
Size: 1093 460 3049 184 2007
Fragments arranged by size:
3049 2007 1093 460 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4971
Fragments arranged by size:
4971 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2695 3038 3527 3893 4751 5110 5269
Size: 648 1529 343 489 366 858 359 159
Cuts at: 5269 518
Size: 2042
Fragments arranged by size:
2042 1529 858 648 489 366 359 343
159
MspA1I CmG'CkG
Cuts at: 1606 2768 3202 4209 4886 6034 6279 1606
Size: 1162 434 1007 677 1148 245 2120
Fragments arranged by size:
2120 1162 1148 1007 677 434 245
NaeI GCC'GGC
Cuts at: 4016 4016
Size: 6793
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6241
NdeI CA'TA_TG
Cuts at: 387 3037 3337 387
Size: 2650 300 3843
Fragments arranged by size:
3843 2650 300
NgoMIV G'CCGG_C
Cuts at: 4014 4014
Size: 6793
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6793
NotI GC'GGCC_GC
Cuts at: 4240 4240
Size: 6793
NspI r_CATG'y
Cuts at: 1132 1229 2568 3006 4151 5696 1132
Size: 97 1339 438 1145 1545 2229
Fragments arranged by size:
2229 1545 1339 1145 438 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6793
PciI A'CATG_T
Cuts at: 5692 5692
Size: 6793
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6793
PfoI T'CCnGG_A
Cuts at: 2502 3645 2502
Size: 1143 5650
PmeI GTTT'AAAC
Cuts at: 4097 4097
Size: 6793
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4862 4894 6375 6407 4862
Size: 32 1481 32 5248
Fragments arranged by size:
5248 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3606 1613
Size: 1993 4800
PsiI TTA'TAA
Cuts at: 4363 4363
Size: 6793
PstI C_TGCA'G
Cuts at: 830 2442 4145 830
Size: 1612 1703 3478
Fragments arranged by size:
3478 1703 1612
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6793
PvuII CAG'CTG
Cuts at: 3202 3202
Size: 6793
SacI G_AGCT'C
Cuts at: 3267 3267
Size: 6793
SalI G'TCGA_C
Cuts at: 1936 4135 1936
Size: 2199 4594
SbfI CC_TGCA'GG
Cuts at: 4145 4145
Size: 6793
ScaI AGT'ACT
Cuts at: 5041 6753 5041
Size: 1712 5081
SfcI C'TryA_G
Cuts at: 826 1045 2438 2604 3253 4141 5276 5957 6148
Size: 219 1393 166 649 888 1135 681 191
Cuts at: 6148 826
Size: 1471
Fragments arranged by size:
1471 1393 1135 888 681 649 219 191
166
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6793
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6793
SmaI CCC'GGG
Cuts at: 1872 4120 1872
Size: 2248 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3593 3800 4897 5798 6060 820
Size: 197 926 1650 207 1097 901 262 1553
Fragments arranged by size:
1650 1553 1097 926 901 262 207 197
SnaBI TAC'GTA
Cuts at: 493 4584 493
Size: 4091 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6793
SphI G_CATG'C
Cuts at: 1132 3006 4151 1132
Size: 1874 1145 3774
Fragments arranged by size:
3774 1874 1145
SspI AAT'ATT
Cuts at: 5 52 2386 2733 2749 4717 5
Size: 47 2334 347 16 1968 2081
Fragments arranged by size:
2334 2081 1968 347 47 16
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6793
StyI C'CwwG_G
Cuts at: 513 1065 4038 4232 513
Size: 552 2973 194 3074
Fragments arranged by size:
3074 2973 552 194
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3969 4820 4837 4990 5175 5620 325
Size: 1255 2389 851 17 153 185 445 1498
Fragments arranged by size:
2389 1498 1255 851 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2740 2902 3990
Size: 274 80 33 51 1443 763 162 1088
Cuts at: 3990 5039 6751 96
Size: 1049 1712 138
Fragments arranged by size:
1712 1443 1088 1049 763 274 162 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6753 6785 6753
Size: 32 6761
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6793
XbaI T'CTAG_A
Cuts at: 4129 4129
Size: 6793
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6793
XmaI C'CCGG_G
Cuts at: 1870 4118 1870
Size: 2248 4545
XmnI GAAnn'nnTTC
Cuts at: 2177 3638 3830 4922 2177
Size: 1461 192 1092 4048
Fragments arranged by size:
4048 1461 1092 192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4601 276
Size: 53 83 186 936 3067 2468
Fragments arranged by size:
3067 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII EcoNI FseI
FspAI KasI NarI NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI