Restriction map for
pFN21AE3272
(Circular) (Six-base) MAPSORT of: pFN21AE3272.seq Check: 1289 from: 1 to: 6730
pFN21AE3272 6730 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2161 2541 2949 2958 3078 2161
Size: 380 408 9 120 5813
Fragments arranged by size:
5813 408 380 120 9
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3438 4540 278
Size: 53 83 186 936 1902 1102 2468
Fragments arranged by size:
2468 1902 1102 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4073 1937
Size: 2136 4594
Acc65I G'GTAC_C
Cuts at: 4051 4051
Size: 6730
AclI AA'CG_TT
Cuts at: 1563 4857 5230 1563
Size: 3294 373 3063
Fragments arranged by size:
3294 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2210 2261 3889 4793 6177 6445 1632
Size: 578 51 1628 904 1384 268 1917
Fragments arranged by size:
1917 1628 1384 904 578 268 51
AfeI AGC'GCT
Cuts at: 3848 3848
Size: 6730
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6533
AflIII A'CryG_T
Cuts at: 1203 2466 3021 5629 6606 1203
Size: 1263 555 2608 977 1327
Fragments arranged by size:
2608 1327 1263 977 555
AgeI A'CCGG_T
Cuts at: 5561 5561
Size: 6730
AhdI GACnn_n'nnGTC
Cuts at: 5459 5459
Size: 6730
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6730
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6730
AlwNI CAG_nnn'CTG
Cuts at: 2968 3049 3238 4473 6045 2968
Size: 81 189 1235 1572 3653
Fragments arranged by size:
3653 1572 1235 189 81
ApaI G_GGCC'C
Cuts at: 2015 2048 2449 3327 3475 3881 3971 2015
Size: 33 401 878 148 406 90 4774
Fragments arranged by size:
4774 878 406 401 148 90 33
ApaLI G'TGCA_C
Cuts at: 3443 4787 5943 3443
Size: 1344 1156 4230
Fragments arranged by size:
4230 1344 1156
ApoI r'AATT_y
Cuts at: 1493 3653 4039 4265 4417 6526 1493
Size: 2160 386 226 152 2109 1697
Fragments arranged by size:
2160 2109 1697 386 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5284 160
Size: 872 4252 1606
Fragments arranged by size:
4252 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
AvaI C'yCGr_G
Cuts at: 1870 1943 2041 2525 3399 3466 3873 3995 4055
Size: 73 98 484 874 67 407 122 60
Cuts at: 4055 1870
Size: 4545
Fragments arranged by size:
4545 874 484 407 122 98 73 67
60
BamHI G'GATC_C
Cuts at: 4060 4427 4060
Size: 367 6363
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2058 2177 2529 2946 3033
Size: 325 217 627 271 119 352 417 87
Cuts at: 3033 3756 4051 5506 618
Size: 723 295 1455 1842
Fragments arranged by size:
1842 1455 723 627 417 352 325 295
271 217 119 87
BanII G_rGCy'C
Cuts at: 1391 2015 2040 2048 2449 3111 3327 3475 3780
Size: 624 25 8 401 662 216 148 305
Cuts at: 3780 3881 3971 4002 4049 1391
Size: 101 90 31 47 4072
Fragments arranged by size:
4072 662 624 401 305 216 148 101
90 47 31 25 8
BbeI G_GCGC'C
Cuts at: 2062 3037 2062
Size: 975 5755
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2198 2776 2831 3471 928
Size: 971 299 578 55 640 4187
Fragments arranged by size:
4187 971 640 578 299 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2360 2394 2822 2856 4921 4955 1903
Size: 34 423 34 428 34 2065 34 3678
Fragments arranged by size:
3678 2065 428 423 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4622 5832 4622
Size: 1210 5520
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6730
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2220 3212 5341
Size: 107 122 71 631 605 548 992 2129
Cuts at: 5341 136
Size: 1525
Fragments arranged by size:
2129 1525 992 631 605 548 122 107
71
BglII A'GATC_T
Cuts at: 3075 6725 3075
Size: 3650 3080
BlpI GC'TnA_GC
Cuts at: 3239 3930 4147 3239
Size: 691 217 5822
Fragments arranged by size:
5822 691 217
Bme1580I G_kGCm'C
Cuts at: 1790 2015 2048 2180 2449 2532 3327 3447 3475
Size: 225 33 132 269 83 795 120 28
Cuts at: 3475 3759 3881 3971 4791 5947 1790
Size: 284 122 90 820 1156 2573
Fragments arranged by size:
2573 1156 820 795 284 269 225 132
122 120 90 83 33 28
BmgBI CAC'GTC
Cuts at: 1318 2782 2803 1318
Size: 1464 21 5245
Fragments arranged by size:
5245 1464 21
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4461 5419 442
Size: 416 53 83 399 24 3044 958 1753
Fragments arranged by size:
3044 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2053 2123 3022 3296 3623 3814 5390 1239
Size: 814 70 899 274 327 191 1576 2579
Fragments arranged by size:
2579 1576 899 814 327 274 191 70
Bpu10I CC'TnA_GC
Cuts at: 2721 2849 2721
Size: 128 6602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 4855 5720 6018 2336
Size: 2519 865 298 3048
Fragments arranged by size:
3048 2519 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2546 3242 5393 882
Size: 617 1047 696 2151 2219
Fragments arranged by size:
2219 2151 1047 696 617
BsaAI yAC'GTr
Cuts at: 493 1204 3022 3448 4521 493
Size: 711 1818 426 1073 2702
Fragments arranged by size:
2702 1818 1073 711 426
BsaBI GATnn'nnATC
Cuts at: 6724 6724
Size: 6730
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2059 2081 3034 3435
Size: 53 83 186 936 526 22 953 401
Cuts at: 3435 4537 4919 5554 275
Size: 1102 382 635 1451
Fragments arranged by size:
1451 1102 953 936 635 526 401 382
186 83 53 22
BsaWI w'CCGG_w
Cuts at: 5163 5561 5835 5982 6393 5163
Size: 398 274 147 411 5500
Fragments arranged by size:
5500 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2415 2445 3812 3842 1178
Size: 30 165 30 1012 30 1367 30 4066
Fragments arranged by size:
4066 1367 1012 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2764 3370 3406 3503 1186
Size: 1578 606 36 97 4413
Fragments arranged by size:
4413 1578 606 97 36
BseYI C'CCAG_C
Cuts at: 2218 2608 3037 3326 3618 4002 5933 6429 2218
Size: 390 429 289 292 384 1931 496 2519
Fragments arranged by size:
2519 1931 496 429 390 384 292 289
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2189 3944 1163
Size: 573 453 1755 3949
Fragments arranged by size:
3949 1755 573 453
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2031 2052 2254 2617 2931 3154
Size: 275 186 30 21 202 363 314 223
Cuts at: 3154 3591 4180 4941 5969 1540
Size: 437 589 761 1028 2301
Fragments arranged by size:
2301 1028 761 589 437 363 314 275
223 202 186 30 21
BsiHKAI G_wGCw'C
Cuts at: 2040 2250 3111 3150 3447 4791 4876 5947 2040
Size: 210 861 39 297 1344 85 1071 2823
Fragments arranged by size:
2823 1344 1071 861 297 210 85 39
BsmI GAATG_Cn'
Cuts at: 4241 4334 4241
Size: 93 6637
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2015 2040 2048 2180 2250 2449 2532
Size: 399 225 25 8 132 70 199 83
Cuts at: 2532 3111 3150 3327 3447 3475 3759 3780 3881
Size: 579 39 177 120 28 284 21 101
Cuts at: 3881 3971 4002 4049 4791 4876 5947 1391
Size: 90 31 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 399 284 225 199
177 132 120 101 90 85 83 70
47 39 31 28 25 21 8
BspEI T'CCGG_A
Cuts at: 6393 6393
Size: 6730
BspHI T'CATG_A
Cuts at: 4619 6349 4619
Size: 1730 5000
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2161 2541 2949 2958 3078 3267 4085 844
Size: 1317 380 408 9 120 189 818 3489
Fragments arranged by size:
3489 1317 818 408 380 189 120 9
BsrBI CCG'CTC
Cuts at: 1394 1823 3280 4617 1394
Size: 429 1457 1337 3507
Fragments arranged by size:
3507 1457 1337 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2715 2955 3754 5225 5399 65
Size: 1183 1467 240 799 1471 174 1396
Fragments arranged by size:
1471 1467 1396 1183 799 240 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3194 3687 5374 5561 1700
Size: 110 1384 493 1687 187 2869
Fragments arranged by size:
2869 1687 1384 493 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6730
BssHII G'CGCG_C
Cuts at: 1925 2923 1925
Size: 998 5732
BssSI C'ACGA_G
Cuts at: 3457 4790 5802 3457
Size: 1333 1012 4385
Fragments arranged by size:
4385 1333 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3145 794
Size: 2351 4379
BstBI TT'CG_AA
Cuts at: 5534 5534
Size: 6730
BstEII G'GTnAC_C
Cuts at: 1184 2303 1184
Size: 1119 5611
BstYI r'GATC_y
Cuts at: 1972 3075 3955 4060 4427 4812 4829 6281 6367
Size: 1103 880 105 367 385 17 1452 86
Cuts at: 6367 6379 6725 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1103 880 385 367 346 105
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2025 3027 3100 3585 3769 513
Size: 552 939 21 1002 73 485 184 3474
Fragments arranged by size:
3474 1002 939 552 485 184 73 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2392 3652 487
Size: 683 56 1166 1260 3565
Fragments arranged by size:
3565 1260 1166 683 56
BtsI GCAGTG_nn'
Cuts at: 2868 4016 4246 5040 5060 2868
Size: 1148 230 794 20 4538
Fragments arranged by size:
4538 1148 794 230 20
ClaI AT'CG_AT
Cuts at: 1554 3837 1554
Size: 2283 4447
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2311 2346 529
Size: 35 1747 35 4913
Fragments arranged by size:
4913 1747 35 35
DraI TTT'AAA
Cuts at: 4034 4034
Size: 6730
DraIII CAC_nnn'GTG
Cuts at: 2201 2201
Size: 6730
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5737 809
Size: 507 4421 1802
Fragments arranged by size:
4421 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2028 2049 2251
Size: 54 1425 50 275 18 198 21 202
Cuts at: 2251 2712 2805 2928 2970 3151 3192 3562 3588
Size: 461 93 123 42 181 41 370 26
Cuts at: 3588 4177 5066 6695 8
Size: 589 889 1629 43
Fragments arranged by size:
1629 1425 889 589 461 370 275 202
198 181 123 93 54 50 43 42
41 26 21 18
EagI C'GGCC_G
Cuts at: 1537 1812 2028 2049 2251 2928 3151 3588 4177
Size: 275 216 21 202 677 223 437 589
Cuts at: 4177 1537
Size: 4090
Fragments arranged by size:
4090 677 589 437 275 223 216 202
21
EarI CTCTTCn'nnn_
Cuts at: 1353 4660 1353
Size: 3307 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5317 5691 5837 387
Size: 1064 138 3728 374 146 1280
Fragments arranged by size:
3728 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2038 3109 2038
Size: 1071 5659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2053 2123 2210 2261 3022 3296 3623
Size: 393 421 70 87 51 761 274 327
Cuts at: 3623 3814 3889 4793 5390 6177 6445 1239
Size: 191 75 904 597 787 268 1524
Fragments arranged by size:
1524 904 787 761 597 421 393 327
274 268 191 87 75 70 51
EcoNI CCTnn'n_nnAGG
Cuts at: 2561 2600 2561
Size: 39 6691
EcoO109I rG'GnC_Cy
Cuts at: 2185 2356 2445 2446 2521 2634 2754 3046 3256
Size: 171 89 1 75 113 120 292 210
Cuts at: 3256 3323 3396 3471 3472 3877 3878 3967 2185
Size: 67 73 75 1 405 1 89 4948
Fragments arranged by size:
4948 405 292 210 171 120 113 89
89 75 75 73 67 1 1 1
EcoRI G'AATT_C
Cuts at: 3653 4039 4417 3653
Size: 386 378 5966
Fragments arranged by size:
5966 386 378
FspI TGC'GCA
Cuts at: 5236 5236
Size: 6730
HaeII r_GCGC'y
Cuts at: 1678 2062 2102 2261 2368 2710 2797 3037 3432
Size: 384 40 159 107 342 87 240 395
Cuts at: 3432 3850 5877 1678
Size: 418 2027 2531
Fragments arranged by size:
2531 2027 418 395 384 342 240 159
107 87 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5371 5403 5445 5477 1373
Size: 32 135 32 3799 32 42 32 2626
Fragments arranged by size:
3799 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3510 4074 4320 669
Size: 988 281 1572 564 246 3079
Fragments arranged by size:
3079 1572 988 564 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6730
HpaI GTT'AAC
Cuts at: 4320 4320
Size: 6730
KasI G'GCGC_C
Cuts at: 2058 3033 2058
Size: 975 5755
KpnI G_GTAC'C
Cuts at: 4055 4055
Size: 6730
MfeI C'AATT_G
Cuts at: 4329 4329
Size: 6730
MluI A'CGCG_T
Cuts at: 6606 6606
Size: 6730
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5844 6028 1305
Size: 4539 184 2007
Fragments arranged by size:
4539 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2714 3564 10
Size: 54 1425 343 882 850 3176
Fragments arranged by size:
3176 1425 882 850 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2274 3125 3747 4688 5047 5206 518
Size: 648 1108 851 622 941 359 159 2042
Fragments arranged by size:
2042 1108 941 851 648 622 359 159
MspA1I CmG'CkG
Cuts at: 1606 2027 2218 2395 3136 3142 3421 3703 3949
Size: 421 191 177 741 6 279 282 246
Cuts at: 3949 4146 4823 5971 6216 1606
Size: 197 677 1148 245 2120
Fragments arranged by size:
2120 1148 741 677 421 282 279 246
245 197 191 177 6
NaeI GCC'GGC
Cuts at: 3196 3689 3196
Size: 493 6237
NarI GG'CG_CC
Cuts at: 2059 3034 2059
Size: 975 5755
NcoI C'CATG_G
Cuts at: 513 1065 2004 3027 3100 513
Size: 552 939 1023 73 4143
Fragments arranged by size:
4143 1023 939 552 73
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6730
NgoMIV G'CCGG_C
Cuts at: 3194 3687 3194
Size: 493 6237
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6730
NotI GC'GGCC_GC
Cuts at: 4177 4177
Size: 6730
NspI r_CATG'y
Cuts at: 1132 1229 3811 4088 5633 1132
Size: 97 2582 277 1545 2229
Fragments arranged by size:
2582 2229 1545 277 97
PasI CC'CwG_GG
Cuts at: 1348 3208 1348
Size: 1860 4870
PciI A'CATG_T
Cuts at: 5629 5629
Size: 6730
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2863 2968 1108
Size: 1755 105 4870
Fragments arranged by size:
4870 1755 105
PfoI T'CCnGG_A
Cuts at: 2069 2495 3830 2069
Size: 426 1335 4969
Fragments arranged by size:
4969 1335 426
PmeI GTTT'AAAC
Cuts at: 4034 4034
Size: 6730
PmlI CAC'GTG
Cuts at: 3448 3448
Size: 6730
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4799 4831 6312 6344 4799
Size: 32 1481 32 5185
Fragments arranged by size:
5185 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2356 2521 3256 2185
Size: 171 165 735 5659
Fragments arranged by size:
5659 735 171 165
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6730
PsiI TTA'TAA
Cuts at: 4300 4300
Size: 6730
PspOMI G'GGCC_C
Cuts at: 2011 2044 2445 3323 3471 3877 3967 2011
Size: 33 401 878 148 406 90 4774
Fragments arranged by size:
4774 878 406 401 148 90 33
PstI C_TGCA'G
Cuts at: 830 2913 3075 3141 4082 830
Size: 2083 162 66 941 3478
Fragments arranged by size:
3478 2083 941 162 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6730
PvuII CAG'CTG
Cuts at: 2218 3136 3142 2218
Size: 918 6 5806
Fragments arranged by size:
5806 918 6
SacI G_AGCT'C
Cuts at: 2040 3111 2040
Size: 1071 5659
SacII CC_GC'GG
Cuts at: 2028 2028
Size: 6730
SalI G'TCGA_C
Cuts at: 1936 4072 1936
Size: 2136 4594
SbfI CC_TGCA'GG
Cuts at: 4082 4082
Size: 6730
ScaI AGT'ACT
Cuts at: 2816 4978 6690 2816
Size: 2162 1712 2856
Fragments arranged by size:
2856 2162 1712
SfcI C'TryA_G
Cuts at: 826 1045 2209 2758 2881 2909 3071 3137 4078
Size: 219 1164 549 123 28 162 66 941
Cuts at: 4078 5213 5894 6085 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1164 1135 941 681 549 219 191
162 123 66 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 3212 3212
Size: 6730
SfoI GGC'GCC
Cuts at: 2060 3035 2060
Size: 975 5755
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6730
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6730
SmaI CCC'GGG
Cuts at: 1872 2043 2527 3401 3875 4057 1872
Size: 171 484 874 474 182 4545
Fragments arranged by size:
4545 874 484 474 182 171
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2351 4834 5735 5997 820
Size: 197 926 408 2483 901 262 1553
Fragments arranged by size:
2483 1553 926 901 408 262 197
SnaBI TAC'GTA
Cuts at: 493 4521 493
Size: 4028 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6730
SphI G_CATG'C
Cuts at: 1132 4088 1132
Size: 2956 3774
SspI AAT'ATT
Cuts at: 5 52 4654 5
Size: 47 4602 2081
Fragments arranged by size:
4602 2081 47
StuI AGG'CCT
Cuts at: 1517 2567 2876 1517
Size: 1050 309 5371
Fragments arranged by size:
5371 1050 309
StyI C'CwwG_G
Cuts at: 513 1065 2004 2322 3027 3100 4169 513
Size: 552 939 318 705 73 1069 3074
Fragments arranged by size:
3074 1069 939 705 552 318 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2672 3662 3755 4757 4774 4927 5112
Size: 1255 1092 990 93 1002 17 153 185
Cuts at: 5112 5557 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1092 1002 990 445 185 153
93 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2814 4976 6688
Size: 274 80 33 51 1443 837 2162 1712
Cuts at: 6688 96
Size: 138
Fragments arranged by size:
2162 1712 1443 837 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2287 2319 2340 2372 3476 3508 6690 6722 2287
Size: 32 21 32 1104 32 3182 32 2295
Fragments arranged by size:
3182 2295 1104 32 32 32 32 21
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6730
XbaI T'CTAG_A
Cuts at: 4066 4066
Size: 6730
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2335 2335
Size: 6730
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6730
XmaI C'CCGG_G
Cuts at: 1870 2041 2525 3399 3873 4055 1870
Size: 171 484 874 474 182 4545
Fragments arranged by size:
4545 874 484 474 182 171
XmnI GAAnn'nnTTC
Cuts at: 4859 4859
Size: 6730
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3436 4538 276
Size: 53 83 186 936 1902 1102 2468
Fragments arranged by size:
2468 1902 1102 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI
NruI NsiI PacI PspXI PsrI RsrII SanDI SapI
SexAI SrfI SwaI