Restriction map for
pFN21AE3270
(Circular) (Six-base) MAPSORT of: pFN21AE3270.seq Check: 7175 from: 1 to: 6724
pFN21AE3270 6724 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2758 3234 2758
Size: 476 6248
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4534 278
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4067 1937
Size: 2130 4594
Acc65I G'GTAC_C
Cuts at: 3573 4045 3573
Size: 472 6252
AclI AA'CG_TT
Cuts at: 1563 4851 5224 1563
Size: 3288 373 3063
Fragments arranged by size:
3288 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3465 3535 4787 6171 6439 1632
Size: 1833 70 1252 1384 268 1917
Fragments arranged by size:
1917 1833 1384 1252 268 70
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6527
AflIII A'CryG_T
Cuts at: 1203 5623 6600 1203
Size: 4420 977 1327
Fragments arranged by size:
4420 1327 977
AgeI A'CCGG_T
Cuts at: 5555 5555
Size: 6724
AhdI GACnn_n'nnGTC
Cuts at: 3079 5453 3079
Size: 2374 4350
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6724
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3410 3442 3410
Size: 32 6692
AlwNI CAG_nnn'CTG
Cuts at: 2350 2385 2677 2815 3169 4467 6039 2350
Size: 35 292 138 354 1298 1572 3035
Fragments arranged by size:
3035 1572 1298 354 292 138 35
ApaI G_GGCC'C
Cuts at: 2796 3358 2796
Size: 562 6162
ApaLI G'TGCA_C
Cuts at: 2805 3062 4781 5937 2805
Size: 257 1719 1156 3592
Fragments arranged by size:
3592 1719 1156 257
ApoI r'AATT_y
Cuts at: 1493 2860 4033 4259 4411 6520 1493
Size: 1367 1173 226 152 2109 1697
Fragments arranged by size:
2109 1697 1367 1173 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5278 160
Size: 872 4246 1606
Fragments arranged by size:
4246 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
AvaI C'yCGr_G
Cuts at: 1870 1943 2112 2466 2789 3288 3487 4049 1870
Size: 73 169 354 323 499 199 562 4545
Fragments arranged by size:
4545 562 499 354 323 199 169 73
BamHI G'GATC_C
Cuts at: 2419 3545 4054 4421 2419
Size: 1126 509 367 4722
Fragments arranged by size:
4722 1126 509 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3573 3906 4045 5500 618
Size: 325 217 627 1786 333 139 1455 1842
Fragments arranged by size:
1842 1786 1455 627 333 325 217 139
BanII G_rGCy'C
Cuts at: 1391 2522 2702 2732 2796 2945 2973 2982 3358
Size: 1131 180 30 64 149 28 9 376
Cuts at: 3358 3820 4043 1391
Size: 462 223 4072
Fragments arranged by size:
4072 1131 462 376 223 180 149 64
30 28 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2497 3267 928
Size: 971 598 770 4385
Fragments arranged by size:
4385 971 770 598
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2282 2316 4915 4949 1903
Size: 34 345 34 2599 34 3678
Fragments arranged by size:
3678 2599 345 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4616 5826 4616
Size: 1210 5514
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6724
BfrBI ATG'CAT
Cuts at: 2199 2199
Size: 6724
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2297 2462 2526
Size: 107 122 71 631 605 625 165 64
Cuts at: 2526 5335 136
Size: 2809 1525
Fragments arranged by size:
2809 1525 631 625 605 165 122 107
71 64
BglII A'GATC_T
Cuts at: 2364 3267 6719 2364
Size: 903 3452 2369
Fragments arranged by size:
3452 2369 903
BlpI GC'TnA_GC
Cuts at: 2622 2780 4141 2622
Size: 158 1361 5205
Fragments arranged by size:
5205 1361 158
Bme1580I G_kGCm'C
Cuts at: 1790 2796 2809 3066 3358 4785 5941 1790
Size: 1006 13 257 292 1427 1156 2573
Fragments arranged by size:
2573 1427 1156 1006 292 257 13
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6724
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2504 3065 3401
Size: 416 53 83 399 24 1087 561 336
Cuts at: 3401 3912 4455 5413 442
Size: 511 543 958 1753
Fragments arranged by size:
1753 1087 958 561 543 511 416 399
336 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6724
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2494 3502 2494
Size: 1008 5716
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2660 2744 2896 2987 3911 5384 1239
Size: 971 450 84 152 91 924 1473 2579
Fragments arranged by size:
2579 1473 971 924 450 152 91 84
Bpu10I CC'TnA_GC
Cuts at: 2516 2939 2516
Size: 423 6301
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2903 3053 4849 5714 6012 2903
Size: 150 1796 865 298 3615
Fragments arranged by size:
3615 1796 865 298 150
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2744 3747 5387 882
Size: 617 1245 1003 1640 2219
Fragments arranged by size:
2219 1640 1245 1003 617
BsaAI yAC'GTr
Cuts at: 493 1204 2833 4515 493
Size: 711 1629 1682 2702
Fragments arranged by size:
2702 1682 1629 711
BsaBI GATnn'nnATC
Cuts at: 6718 6718
Size: 6724
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4531 4913 5548 275
Size: 53 83 186 936 2998 382 635 1451
Fragments arranged by size:
2998 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2048 2646 5157 5555 5829 5976 6387 2048
Size: 598 2511 398 274 147 411 2385
Fragments arranged by size:
2511 2385 598 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2633 2663 2829 2859 3410
Size: 30 165 30 1230 30 166 30 551
Cuts at: 3410 3440 3807 3823 3837 3853 1178
Size: 30 367 16 14 16 4049
Fragments arranged by size:
4049 1230 551 367 166 165 30 30
30 30 30 16 16 14
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3575 1186
Size: 2389 4335
BseYI C'CCAG_C
Cuts at: 2105 2274 2424 2760 5927 6423 2105
Size: 169 150 336 3167 496 2406
Fragments arranged by size:
3167 2406 496 336 169 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2786 3110 3206 3608 1163
Size: 573 1050 324 96 402 4279
Fragments arranged by size:
4279 1050 573 402 324 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3121 4174 4935 5963 1540
Size: 275 186 1120 1053 761 1028 2301
Fragments arranged by size:
2301 1120 1053 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2809 2973 2982 3066 3820 4785 4870 5941 2809
Size: 164 9 84 754 965 85 1071 3592
Fragments arranged by size:
3592 1071 965 754 164 85 84 9
BsmI GAATG_Cn'
Cuts at: 2048 4235 4328 2048
Size: 2187 93 4444
Fragments arranged by size:
4444 2187 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2347 2600 2347
Size: 253 6471
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2522 2702 2732 2796 2809 2945 2973
Size: 399 732 180 30 64 13 136 28
Cuts at: 2973 2982 3066 3358 3820 4043 4785 4870 5941
Size: 9 84 292 462 223 742 85 1071
Cuts at: 5941 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 732 462 399 292 223
180 136 85 84 64 30 28 13
9
BspEI T'CCGG_A
Cuts at: 6387 6387
Size: 6724
BspHI T'CATG_A
Cuts at: 4613 6343 4613
Size: 1730 4994
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2685 2758 3162 3234 3585 4079 844
Size: 1841 73 404 72 351 494 3489
Fragments arranged by size:
3489 1841 494 404 351 73 72
BsrBI CCG'CTC
Cuts at: 1394 1823 4611 1394
Size: 429 2788 3507
Fragments arranged by size:
3507 2788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5219 5393 65
Size: 1183 3971 174 1396
Fragments arranged by size:
3971 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2070 5368 5555 1700
Size: 110 260 3298 187 2869
Fragments arranged by size:
3298 2869 260 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6724
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6724
BssSI C'ACGA_G
Cuts at: 2234 4784 5796 2234
Size: 2550 1012 3162
Fragments arranged by size:
3162 2550 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2229 794
Size: 1435 5289
BstBI TT'CG_AA
Cuts at: 5528 5528
Size: 6724
BstEII G'GTnAC_C
Cuts at: 1184 3640 1184
Size: 2456 4268
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2146
Size: 6724
BstYI r'GATC_y
Cuts at: 1972 2364 2419 3214 3267 3545 4054 4421 4806
Size: 392 55 795 53 278 509 367 385
Cuts at: 4806 4823 6275 6361 6373 6719 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 795 509 392 385 367 346
278 86 55 53 17 12
Bsu36I CC'TnA_GG
Cuts at: 2403 2403
Size: 6724
BtgI C'CryG_G
Cuts at: 513 1065 2460 3511 513
Size: 552 1395 1051 3726
Fragments arranged by size:
3726 1395 1051 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3813 487
Size: 683 56 2587 3398
Fragments arranged by size:
3398 2587 683 56
BtsI GCAGTG_nn'
Cuts at: 3682 3745 4240 5034 5054 3682
Size: 63 495 794 20 5352
Fragments arranged by size:
5352 794 495 63 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6724
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6689
DraI TTT'AAA
Cuts at: 4028 4028
Size: 6724
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5731 809
Size: 507 4415 1802
Fragments arranged by size:
4415 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2262 3118 4171
Size: 54 1425 50 275 18 432 856 1053
Cuts at: 4171 5060 6689 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1053 889 856 432 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3118 4171 1537
Size: 275 1306 1053 4090
Fragments arranged by size:
4090 1306 1053 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2509 3254 3773 4654 1353
Size: 1156 745 519 881 3423
Fragments arranged by size:
3423 1156 881 745 519
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2278 5311 5685 5831 387
Size: 1064 138 689 3033 374 146 1280
Fragments arranged by size:
3033 1280 1064 689 374 146 138
EcoICRI GAG'CTC
Cuts at: 2971 2980 3818 2971
Size: 9 838 5877
Fragments arranged by size:
5877 838 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2660 2744 2896 2987 3465 3535
Size: 393 578 450 84 152 91 478 70
Cuts at: 3535 3911 4787 5384 6171 6439 1239
Size: 376 876 597 787 268 1524
Fragments arranged by size:
1524 876 787 597 578 478 450 393
376 268 152 91 84 70
EcoNI CCTnn'n_nnAGG
Cuts at: 3089 3089
Size: 6724
EcoO109I rG'GnC_Cy
Cuts at: 2674 2793 2890 2959 3073 3355 3806 2674
Size: 119 97 69 114 282 451 5592
Fragments arranged by size:
5592 451 282 119 114 97 69
EcoRI G'AATT_C
Cuts at: 4033 4411 4033
Size: 378 6346
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3676 3676
Size: 6724
FseI GG_CCGG'CC
Cuts at: 2074 2074
Size: 6724
FspI TGC'GCA
Cuts at: 5230 5230
Size: 6724
HaeII r_GCGC'y
Cuts at: 1678 3207 3594 5871 1678
Size: 1529 387 2277 2531
Fragments arranged by size:
2531 2277 1529 387
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2633 2665 3065 3097 3444
Size: 32 135 32 1061 32 400 32 347
Cuts at: 3444 3476 3807 3839 3980 4012 5365 5397 5439
Size: 32 331 32 141 32 1353 32 42
Cuts at: 5439 5471 1373
Size: 32 2626
Fragments arranged by size:
2626 1353 1061 400 347 331 141 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4068 4314 669
Size: 988 281 2130 246 3079
Fragments arranged by size:
3079 2130 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6724
HpaI GTT'AAC
Cuts at: 4314 4314
Size: 6724
KpnI G_GTAC'C
Cuts at: 3577 4049 3577
Size: 472 6252
MfeI C'AATT_G
Cuts at: 4323 4323
Size: 6724
MluI A'CGCG_T
Cuts at: 6600 6600
Size: 6724
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5838 6022 1305
Size: 4533 184 2007
Fragments arranged by size:
4533 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2264 10
Size: 54 1425 343 432 4470
Fragments arranged by size:
4470 1425 432 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2831 2873 4682 5041 5200 518
Size: 648 1665 42 1809 359 159 2042
Fragments arranged by size:
2042 1809 1665 648 359 159 42
MspA1I CmG'CkG
Cuts at: 1606 2219 2274 2760 2854 3412 3672 3714 3852
Size: 613 55 486 94 558 260 42 138
Cuts at: 3852 4140 4817 5965 6210 1606
Size: 288 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 613 558 486 288 260
245 138 94 55 42
NaeI GCC'GGC
Cuts at: 2072 2072
Size: 6724
NcoI C'CATG_G
Cuts at: 513 1065 3511 513
Size: 552 2446 3726
Fragments arranged by size:
3726 2446 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6724
NgoMIV G'CCGG_C
Cuts at: 2070 2070
Size: 6724
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6724
NotI GC'GGCC_GC
Cuts at: 4171 4171
Size: 6724
NsiI A_TGCA'T
Cuts at: 2201 2201
Size: 6724
NspI r_CATG'y
Cuts at: 1132 1229 2103 2248 3404 4082 5627 1132
Size: 97 874 145 1156 678 1545 2229
Fragments arranged by size:
2229 1545 1156 874 678 145 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6724
PciI A'CATG_T
Cuts at: 5623 5623
Size: 6724
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6724
PfoI T'CCnGG_A
Cuts at: 2964 2964
Size: 6724
PmeI GTTT'AAAC
Cuts at: 4028 4028
Size: 6724
PmlI CAC'GTG
Cuts at: 2833 2833
Size: 6724
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3410 3442 3448 3480 4793 4825 6306 6338 3410
Size: 32 6 32 1313 32 1481 32 3796
Fragments arranged by size:
3796 1481 1313 32 32 32 32 6
PpuMI rG'GwC_Cy
Cuts at: 2674 3073 3806 2674
Size: 399 733 5592
Fragments arranged by size:
5592 733 399
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6724
PsiI TTA'TAA
Cuts at: 4294 4294
Size: 6724
PspOMI G'GGCC_C
Cuts at: 2792 3354 2792
Size: 562 6162
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3134 3166 3134
Size: 32 6692
PstI C_TGCA'G
Cuts at: 830 2174 2364 2859 3165 3203 3677 3752 4076
Size: 1344 190 495 306 38 474 75 324
Cuts at: 4076 830
Size: 3478
Fragments arranged by size:
3478 1344 495 474 324 306 190 75
38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6724
PvuII CAG'CTG
Cuts at: 2219 2274 2854 3412 3672 3852 2219
Size: 55 580 558 260 180 5091
Fragments arranged by size:
5091 580 558 260 180 55
SacI G_AGCT'C
Cuts at: 2973 2982 3820 2973
Size: 9 838 5877
Fragments arranged by size:
5877 838 9
SalI G'TCGA_C
Cuts at: 1936 4066 1936
Size: 2130 4594
SbfI CC_TGCA'GG
Cuts at: 4076 4076
Size: 6724
ScaI AGT'ACT
Cuts at: 4972 6684 4972
Size: 1712 5012
SexAI A'CCwGG_T
Cuts at: 2373 3075 3756 2373
Size: 702 681 5341
Fragments arranged by size:
5341 702 681
SfcI C'TryA_G
Cuts at: 826 1045 2170 2360 2798 2855 3161 3199 3673
Size: 219 1125 190 438 57 306 38 474
Cuts at: 3673 3748 4072 5207 5888 6079 826
Size: 75 324 1135 681 191 1471
Fragments arranged by size:
1471 1135 1125 681 474 438 324 306
219 191 190 75 57 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 2462 2462
Size: 6724
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6724
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6724
SmaI CCC'GGG
Cuts at: 1872 2468 2791 3290 4051 1872
Size: 596 323 499 761 4545
Fragments arranged by size:
4545 761 596 499 323
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2918 3068 4828 5729 5991 820
Size: 197 926 975 150 1760 901 262 1553
Fragments arranged by size:
1760 1553 975 926 901 262 197 150
SnaBI TAC'GTA
Cuts at: 493 4515 493
Size: 4022 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6724
SphI G_CATG'C
Cuts at: 1132 4082 1132
Size: 2950 3774
SspI AAT'ATT
Cuts at: 5 52 4648 5
Size: 47 4596 2081
Fragments arranged by size:
4596 2081 47
StuI AGG'CCT
Cuts at: 1517 2562 1517
Size: 1045 5679
StyI C'CwwG_G
Cuts at: 513 1065 2955 3181 3511 3792 4163 513
Size: 552 1890 226 330 281 371 3074
Fragments arranged by size:
3074 1890 552 371 330 281 226
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2266 2357 2752 3385 3537 3894 4751
Size: 1255 686 91 395 633 152 357 857
Cuts at: 4751 4768 4921 5106 5551 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1498 1255 857 686 633 445 395 357
185 153 152 91 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3052 4970 6682
Size: 274 80 33 51 1443 1075 1918 1712
Cuts at: 6682 96
Size: 138
Fragments arranged by size:
1918 1712 1443 1075 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 3823 3855 6684 6716 2829
Size: 32 962 32 2829 32 2837
Fragments arranged by size:
2837 2829 962 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3383 1316
Size: 2067 4657
XbaI T'CTAG_A
Cuts at: 4060 4060
Size: 6724
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2962 3610 3851 3951 2962
Size: 648 241 100 5735
Fragments arranged by size:
5735 648 241 100
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6724
XmaI C'CCGG_G
Cuts at: 1870 2466 2789 3288 4049 1870
Size: 596 323 499 761 4545
Fragments arranged by size:
4545 761 596 499 323
XmnI GAAnn'nnTTC
Cuts at: 4853 4853
Size: 6724
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4532 276
Size: 53 83 186 936 2998 2468
Fragments arranged by size:
2998 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I
DraIII EcoRV FspAI KasI NarI NruI PacI PspXI
RsrII SacII SanDI SapI SfoI SrfI SwaI