Restriction map for
pFN21AE3269
(Circular) (Six-base) MAPSORT of: pFN21AE3269.seq Check: 3918 from: 1 to: 6715
pFN21AE3269 6715 bp
With 182 enzymes: SgfI *
February 10, 2017 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3766 3766
Size: 6715
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2076 3021 3677 4525
Size: 53 83 186 936 540 945 656 848
Cuts at: 4525 278
Size: 2468
Fragments arranged by size:
2468 945 936 848 656 540 186 83
53
AccI GT'mk_AC
Cuts at: 1937 3216 3690 4058 1937
Size: 1279 474 368 4594
Fragments arranged by size:
4594 1279 474 368
Acc65I G'GTAC_C
Cuts at: 4036 4036
Size: 6715
AclI AA'CG_TT
Cuts at: 1563 4842 5215 1563
Size: 3279 373 3063
Fragments arranged by size:
3279 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2885 3223 3466 3515 3554 3838 4778 6162
Size: 1253 338 243 49 39 284 940 1384
Cuts at: 6162 6430 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1253 940 338 284 268 243
49 39
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6518
AflIII A'CryG_T
Cuts at: 1203 2438 2448 3278 3750 5614 6591 1203
Size: 1235 10 830 472 1864 977 1327
Fragments arranged by size:
1864 1327 1235 977 830 472 10
AgeI A'CCGG_T
Cuts at: 3870 5546 3870
Size: 1676 5039
AhdI GACnn_n'nnGTC
Cuts at: 3097 3763 5444 3097
Size: 666 1681 4368
Fragments arranged by size:
4368 1681 666
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6715
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2327 1184
Size: 1143 5572
AlwNI CAG_nnn'CTG
Cuts at: 3409 3885 3919 3983 4458 6030 3409
Size: 476 34 64 475 1572 4094
Fragments arranged by size:
4094 1572 476 475 64 34
ApaI G_GGCC'C
Cuts at: 2052 3197 3436 3580 2052
Size: 1145 239 144 5187
Fragments arranged by size:
5187 1145 239 144
ApaLI G'TGCA_C
Cuts at: 2800 3451 4772 5928 2800
Size: 651 1321 1156 3587
Fragments arranged by size:
3587 1321 1156 651
ApoI r'AATT_y
Cuts at: 1493 4024 4250 4402 6511 1493
Size: 2531 226 152 2109 1697
Fragments arranged by size:
2531 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5269 160
Size: 872 4237 1606
Fragments arranged by size:
4237 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
AvaI C'yCGr_G
Cuts at: 1870 1943 2333 2625 3960 4040 1870
Size: 73 390 292 1335 80 4545
Fragments arranged by size:
4545 1335 390 292 80 73
AvrII C'CTAG_G
Cuts at: 3133 3133
Size: 6715
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3265 3298 3265
Size: 33 6682
BamHI G'GATC_C
Cuts at: 4045 4412 4045
Size: 367 6348
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2631 3728 3803 4036 5491
Size: 325 217 627 844 1097 75 233 1455
Cuts at: 5491 618
Size: 1842
Fragments arranged by size:
1842 1455 1097 844 627 325 233 217
75
BanII G_rGCy'C
Cuts at: 1391 2012 2052 2556 3009 3197 3436 3552 3580
Size: 621 40 504 453 188 239 116 28
Cuts at: 3580 4034 1391
Size: 454 4072
Fragments arranged by size:
4072 621 504 454 453 239 188 116
40 28
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2407 3308 3844 928
Size: 971 508 901 536 3799
Fragments arranged by size:
3799 971 901 536 508
BbvCI CC'TCA_GC
Cuts at: 3150 3150
Size: 6715
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2791 2825 4906 4940 1903
Size: 34 854 34 2081 34 3678
Fragments arranged by size:
3678 2081 854 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4607 5817 4607
Size: 1210 5505
BclI T'GATC_A
Cuts at: 1548 2430 1548
Size: 882 5833
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2574 3192 3321
Size: 107 122 71 631 605 902 618 129
Cuts at: 3321 3584 3801 5326 136
Size: 263 217 1525 1525
Fragments arranged by size:
1525 1525 902 631 618 605 263 217
129 122 107 71
BglII A'GATC_T
Cuts at: 3156 3630 6710 3156
Size: 474 3080 3161
Fragments arranged by size:
3161 3080 474
BlpI GC'TnA_GC
Cuts at: 2651 3044 4132 2651
Size: 393 1088 5234
Fragments arranged by size:
5234 1088 393
Bme1580I G_kGCm'C
Cuts at: 1790 2026 2052 2448 2636 2804 3197 3436 3455
Size: 236 26 396 188 168 393 239 19
Cuts at: 3455 3527 3580 3806 4776 5932 1790
Size: 72 53 226 970 1156 2573
Fragments arranged by size:
2573 1156 970 396 393 239 236 226
188 168 72 53 26 19
BmgBI CAC'GTC
Cuts at: 1318 3664 1318
Size: 2346 4369
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3577 4446 5404
Size: 416 53 83 399 24 2160 869 958
Cuts at: 5404 442
Size: 1753
Fragments arranged by size:
2160 1753 958 869 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6715
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2395 2395
Size: 6715
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2765 2861 3023 3329 3907 5375 1239
Size: 937 589 96 162 306 578 1468 2579
Fragments arranged by size:
2579 1468 937 589 578 306 162 96
Bpu10I CC'TnA_GC
Cuts at: 2011 2732 3150 2011
Size: 721 418 5576
Fragments arranged by size:
5576 721 418
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2741 2993 4840 5705 6003 2741
Size: 252 1847 865 298 3453
Fragments arranged by size:
3453 1847 865 298 252
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2825 3466 5378 882
Size: 617 1326 641 1912 2219
Fragments arranged by size:
2219 1912 1326 641 617
BsaAI yAC'GTr
Cuts at: 493 1204 2439 2911 3751 3799 4506 493
Size: 711 1235 472 840 48 707 2702
Fragments arranged by size:
2702 1235 840 711 707 472 48
BsaBI GATnn'nnATC
Cuts at: 6709 6709
Size: 6715
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2073 3018 3674 3833
Size: 53 83 186 936 540 945 656 159
Cuts at: 3833 4522 4904 5539 275
Size: 689 382 635 1451
Fragments arranged by size:
1451 945 936 689 656 635 540 382
186 159 83 53
BsaWI w'CCGG_w
Cuts at: 3870 5148 5546 5820 5967 6378 3870
Size: 1278 398 274 147 411 4207
Fragments arranged by size:
4207 1278 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2024 2054 2996 3026 3596
Size: 30 165 30 621 30 942 30 570
Cuts at: 3596 3626 1178
Size: 30 4267
Fragments arranged by size:
4267 942 621 570 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2176 2179 2237 2351 2354 2357 2360 3238
Size: 990 3 58 114 3 3 3 878
Cuts at: 3238 3454 1186
Size: 216 4447
Fragments arranged by size:
4447 990 878 216 114 58 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2051 2714 2959 5918 6414 2051
Size: 663 245 2959 496 2352
Fragments arranged by size:
2959 2352 663 496 245
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2273 3364 3892 1163
Size: 573 537 1091 528 3986
Fragments arranged by size:
3986 1091 573 537 528
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3871 4165 4926 5954 1540
Size: 275 186 1870 294 761 1028 2301
Fragments arranged by size:
2301 1870 1028 761 294 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 2556 2772 2804 3009 3455 3480 3552 4776
Size: 544 216 32 205 446 25 72 1224
Cuts at: 4776 4861 5932 2012
Size: 85 1071 2795
Fragments arranged by size:
2795 1224 1071 544 446 216 205 85
72 32 25
BsmI GAATG_Cn'
Cuts at: 4226 4319 4226
Size: 93 6622
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2026 2052 2448 2556 2636 2772
Size: 399 222 14 26 396 108 80 136
Cuts at: 2772 2804 3009 3197 3436 3455 3480 3527 3552
Size: 32 205 188 239 19 25 47 25
Cuts at: 3552 3580 3806 4034 4776 4861 5932 1391
Size: 28 226 228 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 396 239 228 226
222 205 188 136 108 85 80 47
32 28 26 25 25 19 14
BspEI T'CCGG_A
Cuts at: 6378 6378
Size: 6715
BspHI T'CATG_A
Cuts at: 4604 6334 4604
Size: 1730 4985
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3240 3417 3766 4070 844
Size: 2396 177 349 304 3489
Fragments arranged by size:
3489 2396 349 304 177
BsrBI CCG'CTC
Cuts at: 1394 1823 2259 2283 4602 1394
Size: 429 436 24 2319 3507
Fragments arranged by size:
3507 2319 436 429 24
BsrDI GCAATG_nn'
Cuts at: 65 1248 3696 5210 5384 65
Size: 1183 2448 1514 174 1396
Fragments arranged by size:
2448 1514 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2568 3870 5359 5546 1700
Size: 110 758 1302 1489 187 2869
Fragments arranged by size:
2869 1489 1302 758 187 110
BsrGI T'GTAC_A
Cuts at: 96 3603 96
Size: 3507 3208
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6715
BssSI C'ACGA_G
Cuts at: 2669 2900 4775 5787 2669
Size: 231 1875 1012 3597
Fragments arranged by size:
3597 1875 1012 231
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3919 794
Size: 3125 3590
BstBI TT'CG_AA
Cuts at: 5519 5519
Size: 6715
BstEII G'GTnAC_C
Cuts at: 1184 3227 1184
Size: 2043 4672
BstYI r'GATC_y
Cuts at: 1972 3156 3630 4045 4412 4797 4814 6266 6352
Size: 1184 474 415 367 385 17 1452 86
Cuts at: 6352 6364 6710 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1184 474 415 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3878 3878
Size: 6715
BtgI C'CryG_G
Cuts at: 513 1065 2213 2490 2511 3222 513
Size: 552 1148 277 21 711 4006
Fragments arranged by size:
4006 1148 711 552 277 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2512 487
Size: 683 56 1286 4690
Fragments arranged by size:
4690 1286 683 56
BtsI GCAGTG_nn'
Cuts at: 2170 2327 2563 3943 4231 5025 5045 2170
Size: 157 236 1380 288 794 20 3840
Fragments arranged by size:
3840 1380 794 288 236 157 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6715
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6680
DraI TTT'AAA
Cuts at: 4019 4019
Size: 6715
DraIII CAC_nnn'GTG
Cuts at: 2439 3475 3571 2439
Size: 1036 96 5583
Fragments arranged by size:
5583 1036 96
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5722 809
Size: 507 4406 1802
Fragments arranged by size:
4406 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2535 4162 5051
Size: 54 1425 50 275 18 705 1627 889
Cuts at: 5051 6680 8
Size: 1629 43
Fragments arranged by size:
1629 1627 1425 889 705 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4162 1537
Size: 275 2350 4090
Fragments arranged by size:
4090 2350 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2561 2990 3242 3485 3977 4645 1353
Size: 1208 429 252 243 492 668 3423
Fragments arranged by size:
3423 1208 668 492 429 252 243
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2257 5302 5676 5822 387
Size: 1064 138 668 3045 374 146 1280
Fragments arranged by size:
3045 1280 1064 668 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 2554 3007 3550 2010
Size: 544 453 543 5175
Fragments arranged by size:
5175 544 543 453
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2765 2861 2885 3023 3223 3329
Size: 393 544 589 96 24 138 200 106
Cuts at: 3329 3466 3515 3554 3838 3907 4778 5375 6162
Size: 137 49 39 284 69 871 597 787
Cuts at: 6162 6430 1239
Size: 268 1524
Fragments arranged by size:
1524 871 787 597 589 544 393 284
268 200 138 137 106 96 69 49
39 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3413 3413
Size: 6715
EcoO109I rG'GnC_Cy
Cuts at: 2048 2860 3052 3174 3193 3194 3577 2048
Size: 812 192 122 19 1 383 5186
Fragments arranged by size:
5186 812 383 192 122 19 1
EcoRI G'AATT_C
Cuts at: 4024 4402 4024
Size: 378 6337
FspI TGC'GCA
Cuts at: 5221 5221
Size: 6715
HaeII r_GCGC'y
Cuts at: 1678 2658 5862 1678
Size: 980 3204 2531
Fragments arranged by size:
3204 2531 980
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3083 3115 3749 3781 5356
Size: 32 135 32 1511 32 634 32 1575
Cuts at: 5356 5388 5430 5462 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1575 1511 634 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4059 4305 669
Size: 988 281 2121 246 3079
Fragments arranged by size:
3079 2121 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6715
HpaI GTT'AAC
Cuts at: 4305 4305
Size: 6715
KpnI G_GTAC'C
Cuts at: 4040 4040
Size: 6715
MfeI C'AATT_G
Cuts at: 4314 4314
Size: 6715
MluI A'CGCG_T
Cuts at: 6591 6591
Size: 6715
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2286 5829 6013 1305
Size: 981 3543 184 2007
Fragments arranged by size:
3543 2007 981 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4893
Fragments arranged by size:
4893 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2531 3266 3389 3701 3749 4673 5032
Size: 648 1365 735 123 312 48 924 359
Cuts at: 5032 5191 518
Size: 159 2042
Fragments arranged by size:
2042 1365 924 735 648 359 312 159
123 48
MspA1I CmG'CkG
Cuts at: 1606 2215 2595 2679 2718 2728 3366 3486 3609
Size: 609 380 84 39 10 638 120 123
Cuts at: 3609 4131 4808 5956 6201 1606
Size: 522 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 638 609 522 380 245
123 120 84 39 10
NaeI GCC'GGC
Cuts at: 2570 2570
Size: 6715
NcoI C'CATG_G
Cuts at: 513 1065 3222 513
Size: 552 2157 4006
Fragments arranged by size:
4006 2157 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6715
NgoMIV G'CCGG_C
Cuts at: 2568 2568
Size: 6715
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6715
NotI GC'GGCC_GC
Cuts at: 4162 4162
Size: 6715
NspI r_CATG'y
Cuts at: 1132 1229 2236 2308 3282 4073 5618 1132
Size: 97 1007 72 974 791 1545 2229
Fragments arranged by size:
2229 1545 1007 974 791 97 72
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6715
PciI A'CATG_T
Cuts at: 3278 5614 3278
Size: 2336 4379
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6715
PfoI T'CCnGG_A
Cuts at: 3021 3021
Size: 6715
PmeI GTTT'AAAC
Cuts at: 4019 4019
Size: 6715
PmlI CAC'GTG
Cuts at: 2439 2911 3751 3799 2439
Size: 472 840 48 5355
Fragments arranged by size:
5355 840 472 48
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4784 4816 6297 6329 4784
Size: 32 1481 32 5170
Fragments arranged by size:
5170 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3052 3174 3052
Size: 122 6593
PshAI GACnn'nnGTC
Cuts at: 1613 3848 1613
Size: 2235 4480
PsiI TTA'TAA
Cuts at: 4285 4285
Size: 6715
PspOMI G'GGCC_C
Cuts at: 2048 3193 3432 3576 2048
Size: 1145 239 144 5187
Fragments arranged by size:
5187 1145 239 144
PstI C_TGCA'G
Cuts at: 830 2177 2411 2880 3376 3414 3672 4067 830
Size: 1347 234 469 496 38 258 395 3478
Fragments arranged by size:
3478 1347 496 469 395 258 234 38
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6715
PvuII CAG'CTG
Cuts at: 2679 2718 2679
Size: 39 6676
SacI G_AGCT'C
Cuts at: 2012 2556 3009 3552 2012
Size: 544 453 543 5175
Fragments arranged by size:
5175 544 543 453
SacII CC_GC'GG
Cuts at: 2216 2216
Size: 6715
SalI G'TCGA_C
Cuts at: 1936 4057 1936
Size: 2121 4594
SapI GCTCTTCn'nnn_
Cuts at: 2561 2990 3242 3485 2561
Size: 429 252 243 5791
Fragments arranged by size:
5791 429 252 243
SbfI CC_TGCA'GG
Cuts at: 4067 4067
Size: 6715
ScaI AGT'ACT
Cuts at: 4963 6675 4963
Size: 1712 5003
SfcI C'TryA_G
Cuts at: 826 1045 2173 2407 2455 2686 2876 3361 3372
Size: 219 1128 234 48 231 190 485 11
Cuts at: 3372 3410 3668 4063 5198 5879 6070 826
Size: 38 258 395 1135 681 191 1471
Fragments arranged by size:
1471 1135 1128 681 485 395 258 234
231 219 191 190 48 38 11
SfiI GGCCn_nnn'nGGCC
Cuts at: 3192 3192
Size: 6715
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6715
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6715
SmaI CCC'GGG
Cuts at: 1872 3962 4042 1872
Size: 2090 80 4545
Fragments arranged by size:
4545 2090 80
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2756 3008 4819 5720 5982 820
Size: 197 926 813 252 1811 901 262 1553
Fragments arranged by size:
1811 1553 926 901 813 262 252 197
SnaBI TAC'GTA
Cuts at: 493 4506 493
Size: 4013 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6715
SphI G_CATG'C
Cuts at: 1132 2236 4073 1132
Size: 1104 1837 3774
Fragments arranged by size:
3774 1837 1104
SspI AAT'ATT
Cuts at: 5 52 4639 5
Size: 47 4587 2081
Fragments arranged by size:
4587 2081 47
StuI AGG'CCT
Cuts at: 1517 2692 3558 1517
Size: 1175 866 4674
Fragments arranged by size:
4674 1175 866
StyI C'CwwG_G
Cuts at: 513 1065 3133 3222 3768 4154 513
Size: 552 2068 89 546 386 3074
Fragments arranged by size:
3074 2068 552 546 386 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3215 3559 4742 4759 4912 5097 5542
Size: 1255 1635 344 1183 17 153 185 445
Cuts at: 5542 325
Size: 1498
Fragments arranged by size:
1635 1498 1255 1183 445 344 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3603 4961 6673
Size: 274 80 33 51 1443 1626 1358 1712
Cuts at: 6673 96
Size: 138
Fragments arranged by size:
1712 1626 1443 1358 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2282 2314 6675 6707 2282
Size: 32 4361 32 2290
Fragments arranged by size:
4361 2290 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2498 1316
Size: 1182 5533
XbaI T'CTAG_A
Cuts at: 4051 4051
Size: 6715
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2614 3722 2614
Size: 1108 5607
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6715
XmaI C'CCGG_G
Cuts at: 1870 3960 4040 1870
Size: 2090 80 4545
Fragments arranged by size:
4545 2090 80
XmnI GAAnn'nnTTC
Cuts at: 4844 4844
Size: 6715
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2074 3019 3675 4523
Size: 53 83 186 936 540 945 656 848
Cuts at: 4523 276
Size: 2468
Fragments arranged by size:
2468 945 936 848 656 540 186 83
53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstXI
BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PspXI PsrI RsrII SanDI SexAI SfoI
SrfI SwaI