Restriction map for
pFN21AE3267
(Circular) (Six-base) MAPSORT of: pFN21AE3267.seq Check: 4117 from: 1 to: 6691
pFN21AE3267 6691 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4501 278
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4034 1937
Size: 2097 4594
Acc65I G'GTAC_C
Cuts at: 4012 4012
Size: 6691
AclI AA'CG_TT
Cuts at: 1563 4818 5191 1563
Size: 3255 373 3063
Fragments arranged by size:
3255 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3129 4754 6138 6406 1632
Size: 1497 1625 1384 268 1917
Fragments arranged by size:
1917 1625 1497 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6494
AflIII A'CryG_T
Cuts at: 1203 5590 6567 1203
Size: 4387 977 1327
Fragments arranged by size:
4387 1327 977
AgeI A'CCGG_T
Cuts at: 5522 5522
Size: 6691
AhdI GACnn_n'nnGTC
Cuts at: 5420 5420
Size: 6691
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6691
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6691
AlwNI CAG_nnn'CTG
Cuts at: 4434 6006 4434
Size: 1572 5119
ApaLI G'TGCA_C
Cuts at: 4748 5904 4748
Size: 1156 5535
ApoI r'AATT_y
Cuts at: 1493 2412 3209 4000 4226 4378 6487 1493
Size: 919 797 791 226 152 2109 1697
Fragments arranged by size:
2109 1697 919 797 791 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2012 5245 160
Size: 872 980 3233 1606
Fragments arranged by size:
3233 1606 980 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
AvaI C'yCGr_G
Cuts at: 1870 1943 4016 1870
Size: 73 2073 4545
Fragments arranged by size:
4545 2073 73
BamHI G'GATC_C
Cuts at: 2575 3103 4021 4388 2575
Size: 528 918 367 4878
Fragments arranged by size:
4878 918 528 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3039 4012 5467 618
Size: 325 217 627 1252 973 1455 1842
Fragments arranged by size:
1842 1455 1252 973 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2068 2514 4010 1391
Size: 677 446 1496 4072
Fragments arranged by size:
4072 1496 677 446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5720
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2950 2984 4882 4916 1903
Size: 34 1013 34 1898 34 3678
Fragments arranged by size:
3678 1898 1013 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4583 5793 4583
Size: 1210 5481
BclI T'GATC_A
Cuts at: 1548 2440 2923 1548
Size: 892 483 5316
Fragments arranged by size:
5316 892 483
BfrBI ATG'CAT
Cuts at: 2059 2059
Size: 6691
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5302 136
Size: 107 122 71 631 605 3630 1525
Fragments arranged by size:
3630 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6686 6686
Size: 6691
BlpI GC'TnA_GC
Cuts at: 4108 4108
Size: 6691
Bme1580I G_kGCm'C
Cuts at: 1790 2132 3042 3478 4752 5908 1790
Size: 342 910 436 1274 1156 2573
Fragments arranged by size:
2573 1274 1156 910 436 342
BmgBI CAC'GTC
Cuts at: 1318 3926 1318
Size: 2608 4083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3839 4422 5380
Size: 416 53 83 399 24 2422 583 958
Cuts at: 5380 442
Size: 1753
Fragments arranged by size:
2422 1753 958 583 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6691
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 3608 5351 1239
Size: 1247 1122 1743 2579
Fragments arranged by size:
2579 1743 1247 1122
Bpu10I CC'TnA_GC
Cuts at: 2154 3005 2154
Size: 851 5840
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2041 2235 4816 5681 5979 2041
Size: 194 2581 865 298 2753
Fragments arranged by size:
2753 2581 865 298 194
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3584 5354 882
Size: 617 2085 1770 2219
Fragments arranged by size:
2219 2085 1770 617
BsaAI yAC'GTr
Cuts at: 493 1204 4482 493
Size: 711 3278 2702
Fragments arranged by size:
3278 2702 711
BsaBI GATnn'nnATC
Cuts at: 2439 6685 2439
Size: 4246 2445
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4498 4880 5515 275
Size: 53 83 186 936 2965 382 635 1451
Fragments arranged by size:
2965 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5124 5522 5796 5943 6354 5124
Size: 398 274 147 411 5461
Fragments arranged by size:
5461 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 1178
Size: 30 165 30 708 30 5728
Fragments arranged by size:
5728 708 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2309 3014 1186
Size: 1123 705 4863
Fragments arranged by size:
4863 1123 705
BseYI C'CCAG_C
Cuts at: 5894 6390 5894
Size: 496 6195
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2672 1163
Size: 573 936 5182
Fragments arranged by size:
5182 936 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4141 4902 5930 1540
Size: 275 186 2140 761 1028 2301
Fragments arranged by size:
2301 2140 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2068 4752 4837 5908 2068
Size: 2684 85 1071 2851
Fragments arranged by size:
2851 2684 1071 85
BsmI GAATG_Cn'
Cuts at: 2618 3386 3518 4202 4295 2618
Size: 768 132 684 93 5014
Fragments arranged by size:
5014 768 684 132 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2630 2630
Size: 6691
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2132 2514 3042 3478 4010 4752
Size: 399 278 64 382 528 436 532 742
Cuts at: 4752 4837 5908 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 532 528 436 399 382
278 85 64
BspEI T'CCGG_A
Cuts at: 6354 6354
Size: 6691
BspHI T'CATG_A
Cuts at: 3898 4580 6310 3898
Size: 682 1730 4279
Fragments arranged by size:
4279 1730 682
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4046 844
Size: 3202 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4578 1394
Size: 429 2755 3507
Fragments arranged by size:
3507 2755 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5186 5360 65
Size: 1183 3938 174 1396
Fragments arranged by size:
3938 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5335 5522 1700
Size: 110 3525 187 2869
Fragments arranged by size:
3525 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6691
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6691
BssSI C'ACGA_G
Cuts at: 4751 5763 4751
Size: 1012 5679
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6691
BstBI TT'CG_AA
Cuts at: 2204 5495 2204
Size: 3291 3400
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6691
BstYI r'GATC_y
Cuts at: 1972 2575 3103 3888 4021 4388 4773 4790 6242
Size: 603 528 785 133 367 385 17 1452
Cuts at: 6242 6328 6340 6686 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 785 603 528 385 367 346
133 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5200
Fragments arranged by size:
5200 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5952
Fragments arranged by size:
5952 683 56
BtsI GCAGTG_nn'
Cuts at: 2684 2904 3183 4207 5001 5021 2684
Size: 220 279 1024 794 20 4354
Fragments arranged by size:
4354 1024 794 279 220 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6691
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6656
DraI TTT'AAA
Cuts at: 2079 2835 3995 2079
Size: 756 1160 4775
Fragments arranged by size:
4775 1160 756
DraIII CAC_nnn'GTG
Cuts at: 2234 2234
Size: 6691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5698 809
Size: 507 4382 1802
Fragments arranged by size:
4382 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4138 5027 6656
Size: 54 1425 50 275 18 2308 889 1629
Cuts at: 6656 8
Size: 43
Fragments arranged by size:
2308 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4138 1537
Size: 275 2326 4090
Fragments arranged by size:
4090 2326 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4621 1353
Size: 3268 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5278 5652 5798 387
Size: 1064 138 3689 374 146 1280
Fragments arranged by size:
3689 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2066 2066
Size: 6691
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2486 3129 3608 4754 5351 6138 6406
Size: 393 854 643 479 1146 597 787 268
Cuts at: 6406 1239
Size: 1524
Fragments arranged by size:
1524 1146 854 787 643 597 479 393
268
EcoRI G'AATT_C
Cuts at: 4000 4378 4000
Size: 378 6313
EcoRV GAT'ATC
Cuts at: 3763 3763
Size: 6691
FspI TGC'GCA
Cuts at: 5197 5197
Size: 6691
HaeII r_GCGC'y
Cuts at: 1678 5838 1678
Size: 4160 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3880 3912 5332 5364 5406
Size: 32 135 32 2308 32 1420 32 42
Cuts at: 5406 5438 1373
Size: 32 2626
Fragments arranged by size:
2626 2308 1420 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3394 3681 4035 4281 669
Size: 988 281 1456 287 354 246 3079
Fragments arranged by size:
3079 1456 988 354 287 281 246
HindIII A'AGCT_T
Cuts at: 748 2484 748
Size: 1736 4955
HpaI GTT'AAC
Cuts at: 4281 4281
Size: 6691
KpnI G_GTAC'C
Cuts at: 4016 4016
Size: 6691
MfeI C'AATT_G
Cuts at: 4290 4290
Size: 6691
MluI A'CGCG_T
Cuts at: 6567 6567
Size: 6691
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2895 3081 5805 5989 1305
Size: 1590 186 2724 184 2007
Fragments arranged by size:
2724 2007 1590 186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4869
Fragments arranged by size:
4869 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4649 5008 5167 518
Size: 648 3483 359 159 2042
Fragments arranged by size:
3483 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2521 4107 4784 5932 6177 1606
Size: 915 1586 677 1148 245 2120
Fragments arranged by size:
2120 1586 1148 915 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5200
Fragments arranged by size:
5200 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6691
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6691
NotI GC'GGCC_GC
Cuts at: 4138 4138
Size: 6691
NsiI A_TGCA'T
Cuts at: 2061 2061
Size: 6691
NspI r_CATG'y
Cuts at: 1132 1229 2573 2802 2981 4049 5594 1132
Size: 97 1344 229 179 1068 1545 2229
Fragments arranged by size:
2229 1545 1344 1068 229 179 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6691
PciI A'CATG_T
Cuts at: 5590 5590
Size: 6691
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2234 1108
Size: 1126 5565
PfoI T'CCnGG_A
Cuts at: 3507 3507
Size: 6691
PmeI GTTT'AAAC
Cuts at: 3995 3995
Size: 6691
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4760 4792 6273 6305 4760
Size: 32 1481 32 5146
Fragments arranged by size:
5146 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6691
PsiI TTA'TAA
Cuts at: 2360 2723 2951 4261 2360
Size: 363 228 1310 4790
Fragments arranged by size:
4790 1310 363 228
PstI C_TGCA'G
Cuts at: 830 3122 3140 4043 830
Size: 2292 18 903 3478
Fragments arranged by size:
3478 2292 903 18
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6691
PvuII CAG'CTG
Cuts at: 2521 2521
Size: 6691
SacI G_AGCT'C
Cuts at: 2068 2068
Size: 6691
SalI G'TCGA_C
Cuts at: 1936 4033 1936
Size: 2097 4594
SbfI CC_TGCA'GG
Cuts at: 4043 4043
Size: 6691
ScaI AGT'ACT
Cuts at: 3134 4939 6651 3134
Size: 1805 1712 3174
Fragments arranged by size:
3174 1805 1712
SexAI A'CCwGG_T
Cuts at: 2601 2601
Size: 6691
SfcI C'TryA_G
Cuts at: 826 1045 3118 3136 3860 4039 5174 5855 6046
Size: 219 2073 18 724 179 1135 681 191
Cuts at: 6046 826
Size: 1471
Fragments arranged by size:
2073 1471 1135 724 681 219 191 179
18
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6691
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6691
SmaI CCC'GGG
Cuts at: 1872 4018 1872
Size: 2146 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2020 2250 4795 5696 5958 820
Size: 197 926 77 230 2545 901 262 1553
Fragments arranged by size:
2545 1553 926 901 262 230 197 77
SnaBI TAC'GTA
Cuts at: 493 4482 493
Size: 3989 2702
SpeI A'CTAG_T
Cuts at: 152 2531 152
Size: 2379 4312
SphI G_CATG'C
Cuts at: 1132 4049 1132
Size: 2917 3774
SspI AAT'ATT
Cuts at: 5 52 3411 3941 4615 5
Size: 47 3359 530 674 2081
Fragments arranged by size:
3359 2081 674 530 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6691
StyI C'CwwG_G
Cuts at: 513 1065 2004 2132 4130 513
Size: 552 939 128 1998 3074
Fragments arranged by size:
3074 1998 939 552 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3352 4718 4735 4888 5073 5518 325
Size: 1255 1772 1366 17 153 185 445 1498
Fragments arranged by size:
1772 1498 1366 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2801 3132 4937
Size: 274 80 33 51 1443 824 331 1805
Cuts at: 4937 6649 96
Size: 1712 138
Fragments arranged by size:
1805 1712 1443 824 331 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2111 2143 3088 3120 6651 6683 2111
Size: 32 945 32 3531 32 2119
Fragments arranged by size:
3531 2119 945 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6691
XbaI T'CTAG_A
Cuts at: 4027 4027
Size: 6691
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6691
XmaI C'CCGG_G
Cuts at: 1870 4016 1870
Size: 2146 4545
XmnI GAAnn'nnTTC
Cuts at: 3150 4820 3150
Size: 1670 5021
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4499 276
Size: 53 83 186 936 2965 2468
Fragments arranged by size:
2965 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI EcoO109I
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SfiI SfoI SrfI SwaI XcmI