Restriction map for
pFN21AE3264
(Circular) (Six-base) MAPSORT of: pFN21AE3264.seq Check: 4902 from: 1 to: 6634
pFN21AE3264 6634 bp
With 182 enzymes: SgfI *
March 28, 2012 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4444 278
Size: 53 83 186 936 2908 2468
Fragments arranged by size:
2908 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3977 1937
Size: 2040 4594
Acc65I G'GTAC_C
Cuts at: 3955 3955
Size: 6634
AclI AA'CG_TT
Cuts at: 1563 3790 4761 5134 1563
Size: 2227 971 373 3063
Fragments arranged by size:
3063 2227 971 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3831 3899 4697 6081 6349 1632
Size: 2199 68 798 1384 268 1917
Fragments arranged by size:
2199 1917 1384 798 268 68
AfeI AGC'GCT
Cuts at: 2027 2027
Size: 6634
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6437
AflIII A'CryG_T
Cuts at: 1203 2838 2953 5533 6510 1203
Size: 1635 115 2580 977 1327
Fragments arranged by size:
2580 1635 1327 977 115
AgeI A'CCGG_T
Cuts at: 5465 5465
Size: 6634
AhdI GACnn_n'nnGTC
Cuts at: 3876 5363 3876
Size: 1487 5147
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3767 1184
Size: 2583 4051
AlwNI CAG_nnn'CTG
Cuts at: 2233 4377 5949 2233
Size: 2144 1572 2918
Fragments arranged by size:
2918 2144 1572
ApaI G_GGCC'C
Cuts at: 2215 2215
Size: 6634
ApaLI G'TGCA_C
Cuts at: 4691 5847 4691
Size: 1156 5478
ApoI r'AATT_y
Cuts at: 1493 2366 2374 2928 3208 3943 4169 4321 6430
Size: 873 8 554 280 735 226 152 2109
Cuts at: 6430 1493
Size: 1697
Fragments arranged by size:
2109 1697 873 735 554 280 226 152
8
AseI AT'TA_AT
Cuts at: 160 1032 5188 160
Size: 872 4156 1606
Fragments arranged by size:
4156 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6634
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2120 2208 3959 1870
Size: 73 78 99 88 1751 4545
Fragments arranged by size:
4545 1751 99 88 78 73
BamHI G'GATC_C
Cuts at: 3964 4331 3964
Size: 367 6267
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2179 2448 3955 5410
Size: 325 217 627 297 95 269 1507 1455
Cuts at: 5410 618
Size: 1842
Fragments arranged by size:
1842 1507 1455 627 325 297 269 217
95
BanII G_rGCy'C
Cuts at: 1391 2215 2802 3678 3953 1391
Size: 824 587 876 275 4072
Fragments arranged by size:
4072 876 824 587 275
BbeI G_GCGC'C
Cuts at: 2183 2183
Size: 6634
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3089 3696 928
Size: 971 1190 607 3866
Fragments arranged by size:
3866 1190 971 607
BbvCI CC'TCA_GC
Cuts at: 2063 2063
Size: 6634
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3283 3317 4825 4859 1903
Size: 34 50 34 1262 34 1508 34 3678
Fragments arranged by size:
3678 1508 1262 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4526 5736 4526
Size: 1210 5424
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6634
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2067 2188 5245
Size: 107 122 71 631 605 395 121 3057
Cuts at: 5245 136
Size: 1525
Fragments arranged by size:
3057 1525 631 605 395 122 121 107
71
BglII A'GATC_T
Cuts at: 6629 6629
Size: 6634
BlpI GC'TnA_GC
Cuts at: 2111 2301 4051 2111
Size: 190 1750 4694
Fragments arranged by size:
4694 1750 190
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2215 4695 5851 1790
Size: 297 128 2480 1156 2573
Fragments arranged by size:
2573 2480 1156 297 128
BmgBI CAC'GTC
Cuts at: 1318 2751 1318
Size: 1433 5201
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2221 3672 4365
Size: 416 53 83 399 24 804 1451 693
Cuts at: 4365 5323 442
Size: 958 1753
Fragments arranged by size:
1753 1451 958 804 693 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6634
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2712 2712
Size: 6634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2979 3428 3659 3758 5294 1239
Size: 1740 449 231 99 1536 2579
Fragments arranged by size:
2579 1740 1536 449 231 99
Bpu10I CC'TnA_GC
Cuts at: 2063 2169 2063
Size: 106 6528
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4759 5624 5922 4759
Size: 865 298 5471
Fragments arranged by size:
5471 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3232 3288 5297 882
Size: 617 1733 56 2009 2219
Fragments arranged by size:
2219 2009 1733 617 56
BsaAI yAC'GTr
Cuts at: 493 1204 2716 2954 4425 493
Size: 711 1512 238 1471 2702
Fragments arranged by size:
2702 1512 1471 711 238
BsaBI GATnn'nnATC
Cuts at: 6628 6628
Size: 6634
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2180 4441 4823 5458
Size: 53 83 186 936 647 2261 382 635
Cuts at: 5458 275
Size: 1451
Fragments arranged by size:
2261 1451 936 647 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5067 5465 5739 5886 6297 5067
Size: 398 274 147 411 5404
Fragments arranged by size:
5404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2963 2993 1178
Size: 30 165 30 1560 30 4819
Fragments arranged by size:
4819 1560 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3014 3656 3761 1186
Size: 1828 642 105 4059
Fragments arranged by size:
4059 1828 642 105
BseYI C'CCAG_C
Cuts at: 2039 2080 3369 5837 6333 2039
Size: 41 1289 2468 496 2340
Fragments arranged by size:
2468 2340 1289 496 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2462 3773 1163
Size: 573 726 1311 4024
Fragments arranged by size:
4024 1311 726 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2192 2557 2610 4084 4845 5873
Size: 275 186 191 365 53 1474 761 1028
Cuts at: 5873 1540
Size: 2301
Fragments arranged by size:
2301 1474 1028 761 365 275 191 186
53
BsiHKAI G_wGCw'C
Cuts at: 2802 4695 4780 5851 2802
Size: 1893 85 1071 3585
Fragments arranged by size:
3585 1893 1071 85
BsmI GAATG_Cn'
Cuts at: 2646 4145 4238 2646
Size: 1499 93 5042
Fragments arranged by size:
5042 1499 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3638 3638
Size: 6634
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2215 2802 3678 3953 4695 4780
Size: 399 297 128 587 876 275 742 85
Cuts at: 4780 5851 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 876 742 587 399 297 275
128 85
BspEI T'CCGG_A
Cuts at: 6297 6297
Size: 6634
BspHI T'CATG_A
Cuts at: 2911 4523 6253 2911
Size: 1612 1730 3292
Fragments arranged by size:
3292 1730 1612
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2241 3547 3989 844
Size: 1397 1306 442 3489
Fragments arranged by size:
3489 1397 1306 442
BsrBI CCG'CTC
Cuts at: 1394 1823 4521 1394
Size: 429 2698 3507
Fragments arranged by size:
3507 2698 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3387 5129 5303 65
Size: 1183 2139 1742 174 1396
Fragments arranged by size:
2139 1742 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2126 2176 2624 5278 5465 1700
Size: 110 316 50 448 2654 187 2869
Fragments arranged by size:
2869 2654 448 316 187 110 50
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6634
BssHII G'CGCG_C
Cuts at: 1925 2106 1925
Size: 181 6453
BssSI C'ACGA_G
Cuts at: 3702 4694 5706 3702
Size: 992 1012 4630
Fragments arranged by size:
4630 1012 992
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6634
BstBI TT'CG_AA
Cuts at: 5438 5438
Size: 6634
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6634
BstXI CCAn_nnnn'nTGG
Cuts at: 3278 3685 3278
Size: 407 6227
BstYI r'GATC_y
Cuts at: 1972 3964 4331 4716 4733 6185 6271 6283 6629
Size: 1992 367 385 17 1452 86 12 346
Cuts at: 6629 1972
Size: 1977
Fragments arranged by size:
1992 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2551 2635 2868 3900 513
Size: 552 1486 84 233 1032 3247
Fragments arranged by size:
3247 1486 1032 552 233 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2232 487
Size: 683 56 1006 4889
Fragments arranged by size:
4889 1006 683 56
BtsI GCAGTG_nn'
Cuts at: 3577 4150 4944 4964 3577
Size: 573 794 20 5247
Fragments arranged by size:
5247 794 573 20
ClaI AT'CG_AT
Cuts at: 1554 2506 1554
Size: 952 5682
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6599
DraI TTT'AAA
Cuts at: 3938 3938
Size: 6634
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5641 809
Size: 507 4325 1802
Fragments arranged by size:
4325 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2189 2607 2871
Size: 54 1425 50 275 18 359 418 264
Cuts at: 2871 4081 4970 6599 8
Size: 1210 889 1629 43
Fragments arranged by size:
1629 1425 1210 889 418 359 275 264
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2189 2607 4081 1537
Size: 275 377 418 1474 4090
Fragments arranged by size:
4090 1474 418 377 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2021 2255 2337 2427 2679 3576 4564 1353
Size: 668 234 82 90 252 897 988 3423
Fragments arranged by size:
3423 988 897 668 252 234 90 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5221 5595 5741 387
Size: 1064 138 3632 374 146 1280
Fragments arranged by size:
3632 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2800 2800
Size: 6634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2979 3428 3659 3758 3831 3899 4697
Size: 393 1347 449 231 99 73 68 798
Cuts at: 4697 5294 6081 6349 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1347 798 787 597 449 393 268
231 99 73 68
EcoO109I rG'GnC_Cy
Cuts at: 2204 2731 3439 2204
Size: 527 708 5399
Fragments arranged by size:
5399 708 527
EcoRI G'AATT_C
Cuts at: 2928 3943 4321 2928
Size: 1015 378 5241
Fragments arranged by size:
5241 1015 378
FseI GG_CCGG'CC
Cuts at: 2130 2130
Size: 6634
FspI TGC'GCA
Cuts at: 5140 5140
Size: 6634
HaeII r_GCGC'y
Cuts at: 1678 2029 2100 2183 2199 5781 1678
Size: 351 71 83 16 3582 2531
Fragments arranged by size:
3582 2531 351 83 71 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2338 2340 2370 2372 3809
Size: 32 135 32 766 2 30 2 1437
Cuts at: 3809 3841 3862 3894 5275 5307 5349 5381 1373
Size: 32 21 32 1381 32 42 32 2626
Fragments arranged by size:
2626 1437 1381 766 135 42 32 32
32 32 32 32 30 21 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 3013 3978 4224 669
Size: 988 281 1075 965 246 3079
Fragments arranged by size:
3079 1075 988 965 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6634
HpaI GTT'AAC
Cuts at: 4224 4224
Size: 6634
KasI G'GCGC_C
Cuts at: 2179 2179
Size: 6634
KpnI G_GTAC'C
Cuts at: 3959 3959
Size: 6634
MfeI C'AATT_G
Cuts at: 4233 4233
Size: 6634
MluI A'CGCG_T
Cuts at: 6510 6510
Size: 6634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2941 3155 5748 5932 1305
Size: 1636 214 2593 184 2007
Fragments arranged by size:
2593 2007 1636 214 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2873 10
Size: 54 1425 343 1041 3771
Fragments arranged by size:
3771 1425 1041 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 2888 3276 3749 4592 4951 5110
Size: 648 1374 348 388 473 843 359 159
Cuts at: 5110 518
Size: 2042
Fragments arranged by size:
2042 1374 843 648 473 388 359 348
159
MspA1I CmG'CkG
Cuts at: 1606 2011 2067 2896 3448 3520 3902 4050 4727
Size: 405 56 829 552 72 382 148 677
Cuts at: 4727 5875 6120 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 829 677 552 405 382 245
148 72 56
NaeI GCC'GGC
Cuts at: 2128 2178 2128
Size: 50 6584
NarI GG'CG_CC
Cuts at: 2180 2180
Size: 6634
NcoI C'CATG_G
Cuts at: 513 1065 2868 513
Size: 552 1803 4279
Fragments arranged by size:
4279 1803 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6634
NgoMIV G'CCGG_C
Cuts at: 2126 2176 2126
Size: 50 6584
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6634
NotI GC'GGCC_GC
Cuts at: 4081 4081
Size: 6634
NspI r_CATG'y
Cuts at: 1132 1229 2842 3138 3748 3992 5537 1132
Size: 97 1613 296 610 244 1545 2229
Fragments arranged by size:
2229 1613 1545 610 296 244 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6634
PciI A'CATG_T
Cuts at: 2838 5533 2838
Size: 2695 3939
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2428 3036 1108
Size: 1320 608 4706
Fragments arranged by size:
4706 1320 608
PmeI GTTT'AAAC
Cuts at: 3938 3938
Size: 6634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4703 4735 6216 6248 4703
Size: 32 1481 32 5089
Fragments arranged by size:
5089 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2204 2731 3439 2204
Size: 527 708 5399
Fragments arranged by size:
5399 708 527
PshAI GACnn'nnGTC
Cuts at: 1613 2771 3627 1613
Size: 1158 856 4620
Fragments arranged by size:
4620 1158 856
PsiI TTA'TAA
Cuts at: 4204 4204
Size: 6634
PspOMI G'GGCC_C
Cuts at: 2211 2211
Size: 6634
PspXI vC'TCGA_Gb
Cuts at: 2021 2021
Size: 6634
PstI C_TGCA'G
Cuts at: 830 2166 2668 3438 3986 830
Size: 1336 502 770 548 3478
Fragments arranged by size:
3478 1336 770 548 502
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6634
PvuII CAG'CTG
Cuts at: 2896 3448 2896
Size: 552 6082
SacI G_AGCT'C
Cuts at: 2802 2802
Size: 6634
SacII CC_GC'GG
Cuts at: 3903 3903
Size: 6634
SalI G'TCGA_C
Cuts at: 1936 3976 1936
Size: 2040 4594
SanDI GG'GwC_CC
Cuts at: 2204 2204
Size: 6634
SapI GCTCTTCn'nnn_
Cuts at: 2021 2021
Size: 6634
SbfI CC_TGCA'GG
Cuts at: 3986 3986
Size: 6634
ScaI AGT'ACT
Cuts at: 4882 6594 4882
Size: 1712 4922
SfcI C'TryA_G
Cuts at: 826 1045 2162 2664 3219 3434 3982 5117 5798
Size: 219 1117 502 555 215 548 1135 681
Cuts at: 5798 5989 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1117 681 555 548 502 219
215 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2067 2067
Size: 6634
SfoI GGC'GCC
Cuts at: 2181 2181
Size: 6634
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6634
SgrAI Cr'CCGG_yG
Cuts at: 1700 2176 1700
Size: 476 6158
SmaI CCC'GGG
Cuts at: 1872 2210 3961 1872
Size: 338 1751 4545
Fragments arranged by size:
4545 1751 338
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2021 4738 5639 5901 820
Size: 197 926 78 2717 901 262 1553
Fragments arranged by size:
2717 1553 926 901 262 197 78
SnaBI TAC'GTA
Cuts at: 493 2716 4425 493
Size: 2223 1709 2702
Fragments arranged by size:
2702 2223 1709
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6634
SphI G_CATG'C
Cuts at: 1132 3748 3992 1132
Size: 2616 244 3774
Fragments arranged by size:
3774 2616 244
SspI AAT'ATT
Cuts at: 5 52 3154 3481 4558 5
Size: 47 3102 327 1077 2081
Fragments arranged by size:
3102 2081 1077 327 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6634
StyI C'CwwG_G
Cuts at: 513 1065 2649 2868 4073 513
Size: 552 1584 219 1205 3074
Fragments arranged by size:
3074 1584 1205 552 219
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2648 3620 4661 4678 4831 5016 5461
Size: 1255 1068 972 1041 17 153 185 445
Cuts at: 5461 325
Size: 1498
Fragments arranged by size:
1498 1255 1068 1041 972 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4880 6592 96
Size: 274 80 33 51 1443 2903 1712 138
Fragments arranged by size:
2903 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3112 3144 6594 6626 3112
Size: 32 3450 32 3120
Fragments arranged by size:
3450 3120 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2789 1316
Size: 1473 5161
XbaI T'CTAG_A
Cuts at: 3970 3970
Size: 6634
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2875 2875
Size: 6634
XhoI C'TCGA_G
Cuts at: 1943 2021 1943
Size: 78 6556
XmaI C'CCGG_G
Cuts at: 1870 2208 3959 1870
Size: 338 1751 4545
Fragments arranged by size:
4545 1751 338
XmnI GAAnn'nnTTC
Cuts at: 4763 4763
Size: 6634
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4442 276
Size: 53 83 186 936 2908 2468
Fragments arranged by size:
2908 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI
PacI PfoI PmlI PsrI RsrII SexAI SrfI SwaI