Restriction map for
pFN21AE3259
(Circular) (Six-base) MAPSORT of: pFN21AE3259.seq Check: 4315 from: 1 to: 6556
pFN21AE3259 6556 bp
With 182 enzymes: SgfI *
July 6, 2012 17:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2786 3096 2786
Size: 310 6246
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4366 278
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3899 1937
Size: 1962 4594
Acc65I G'GTAC_C
Cuts at: 3488 3877 3488
Size: 389 6167
AclI AA'CG_TT
Cuts at: 1563 4683 5056 1563
Size: 3120 373 3063
Fragments arranged by size:
3120 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3120 3306 4619 6003 6271 1632
Size: 1488 186 1313 1384 268 1917
Fragments arranged by size:
1917 1488 1384 1313 268 186
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6359
AflIII A'CryG_T
Cuts at: 1203 2081 2769 5455 6432 1203
Size: 878 688 2686 977 1327
Fragments arranged by size:
2686 1327 977 878 688
AgeI A'CCGG_T
Cuts at: 5387 5387
Size: 6556
AhdI GACnn_n'nnGTC
Cuts at: 5285 5285
Size: 6556
AleI CACnn'nnGTG
Cuts at: 1166 2348 1166
Size: 1182 5374
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6556
AlwNI CAG_nnn'CTG
Cuts at: 3851 4299 5871 3851
Size: 448 1572 4536
Fragments arranged by size:
4536 1572 448
ApaI G_GGCC'C
Cuts at: 3011 3011
Size: 6556
ApaLI G'TGCA_C
Cuts at: 2069 2773 4613 5769 2069
Size: 704 1840 1156 2856
Fragments arranged by size:
2856 1840 1156 704
ApoI r'AATT_y
Cuts at: 1493 2109 2873 3689 3778 3865 4091 4243 6352
Size: 616 764 816 89 87 226 152 2109
Cuts at: 6352 1493
Size: 1697
Fragments arranged by size:
2109 1697 816 764 616 226 152 89
87
AseI AT'TA_AT
Cuts at: 160 1032 5110 160
Size: 872 4078 1606
Fragments arranged by size:
4078 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
AvaI C'yCGr_G
Cuts at: 1870 1943 3881 1870
Size: 73 1938 4545
Fragments arranged by size:
4545 1938 73
BamHI G'GATC_C
Cuts at: 3886 4253 3886
Size: 367 6189
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3193 3293 3344 3488 3502
Size: 325 217 627 1406 100 51 144 14
Cuts at: 3502 3566 3877 5332 618
Size: 64 311 1455 1842
Fragments arranged by size:
1842 1455 1406 627 325 311 217 144
100 64 51 14
BanII G_rGCy'C
Cuts at: 1391 2653 3011 3069 3875 1391
Size: 1262 358 58 806 4072
Fragments arranged by size:
4072 1262 806 358 58
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5585
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3347 3381 4747 4781 1903
Size: 34 1410 34 1366 34 3678
Fragments arranged by size:
3678 1410 1366 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4448 5658 4448
Size: 1210 5346
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6556
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2790 5167 136
Size: 107 122 71 631 605 1118 2377 1525
Fragments arranged by size:
2377 1525 1118 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3447 6551 3447
Size: 3104 3452
BlpI GC'TnA_GC
Cuts at: 3973 3973
Size: 6556
Bme1580I G_kGCm'C
Cuts at: 1790 2073 2355 2777 3011 3296 3642 4617 5773
Size: 283 282 422 234 285 346 975 1156
Cuts at: 5773 1790
Size: 2573
Fragments arranged by size:
2573 1156 975 422 346 285 283 282
234
BmgBI CAC'GTC
Cuts at: 1318 2686 1318
Size: 1368 5188
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2349 4287 5245
Size: 416 53 83 399 24 932 1938 958
Cuts at: 5245 442
Size: 1753
Fragments arranged by size:
1938 1753 958 932 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6556
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2688 2951 5216 1239
Size: 1449 263 2265 2579
Fragments arranged by size:
2579 2265 1449 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3666 4681 5546 5844 3666
Size: 1015 865 298 4378
Fragments arranged by size:
4378 1015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5219 882
Size: 617 3720 2219
Fragments arranged by size:
3720 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2772 4347 493
Size: 711 1568 1575 2702
Fragments arranged by size:
2702 1575 1568 711
BsaBI GATnn'nnATC
Cuts at: 6550 6550
Size: 6556
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4363 4745 5380 275
Size: 53 83 186 936 2830 382 635 1451
Fragments arranged by size:
2830 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4989 5387 5661 5808 6219 4989
Size: 398 274 147 411 5326
Fragments arranged by size:
5326 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6331
Fragments arranged by size:
6331 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2134 2146 3137 3140 3152 3227 3230 3233
Size: 948 12 991 3 12 75 3 3
Cuts at: 3233 3254 3257 3260 3604 1186
Size: 21 3 3 344 4138
Fragments arranged by size:
4138 991 948 344 75 21 12 12
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3580 5759 6255 3580
Size: 2179 496 3881
Fragments arranged by size:
3881 2179 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2818 3068 1163
Size: 573 1082 250 4651
Fragments arranged by size:
4651 1082 573 250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4006 4767 5795 1540
Size: 275 186 2005 761 1028 2301
Fragments arranged by size:
2301 2005 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2073 2653 2777 2992 4617 4702 5773 2073
Size: 580 124 215 1625 85 1071 2856
Fragments arranged by size:
2856 1625 1071 580 215 124 85
BsmI GAATG_Cn'
Cuts at: 3379 4067 4160 3379
Size: 688 93 5775
Fragments arranged by size:
5775 688 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2073 2355 2653 2777 2992 3011 3069
Size: 399 283 282 298 124 215 19 58
Cuts at: 3069 3296 3642 3875 4617 4702 5773 1391
Size: 227 346 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 346 298 283 282
233 227 215 124 85 58 19
BspEI T'CCGG_A
Cuts at: 6219 6219
Size: 6556
BspHI T'CATG_A
Cuts at: 4445 6175 4445
Size: 1730 4826
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2066 2786 2852 3096 3911 844
Size: 1222 720 66 244 815 3489
Fragments arranged by size:
3489 1222 815 720 244 66
BsrBI CCG'CTC
Cuts at: 1394 1823 4443 1394
Size: 429 2620 3507
Fragments arranged by size:
3507 2620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5051 5225 65
Size: 1183 3803 174 1396
Fragments arranged by size:
3803 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5200 5387 1700
Size: 110 3390 187 2869
Fragments arranged by size:
3390 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2293 96
Size: 2197 4359
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6556
BssSI C'ACGA_G
Cuts at: 2724 4616 5628 2724
Size: 1892 1012 3652
Fragments arranged by size:
3652 1892 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2799 3156 3374 794
Size: 2005 357 218 3976
Fragments arranged by size:
3976 2005 357 218
BstBI TT'CG_AA
Cuts at: 5360 5360
Size: 6556
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6556
BstYI r'GATC_y
Cuts at: 1972 3447 3886 4253 4638 4655 6107 6193 6205
Size: 1475 439 367 385 17 1452 86 12
Cuts at: 6205 6551 1972
Size: 346 1977
Fragments arranged by size:
1977 1475 1452 439 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2474 2474
Size: 6556
BtgI C'CryG_G
Cuts at: 513 1065 3834 513
Size: 552 2769 3235
Fragments arranged by size:
3235 2769 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2114 487
Size: 683 56 888 4929
Fragments arranged by size:
4929 888 683 56
BtsI GCAGTG_nn'
Cuts at: 2091 2197 3084 4072 4866 4886 2091
Size: 106 887 988 794 20 3761
Fragments arranged by size:
3761 988 887 794 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6556
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6521
DraI TTT'AAA
Cuts at: 2115 3860 2115
Size: 1745 4811
DraIII CAC_nnn'GTG
Cuts at: 3200 3200
Size: 6556
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5563 809
Size: 507 4247 1802
Fragments arranged by size:
4247 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4003 4892 6521
Size: 54 1425 50 275 18 2173 889 1629
Cuts at: 6521 8
Size: 43
Fragments arranged by size:
2173 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4003 1537
Size: 275 2191 4090
Fragments arranged by size:
4090 2191 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2641 3129 3225 3231 3321 3400 3549 3622
Size: 1288 488 96 6 90 79 149 73
Cuts at: 3622 3810 4486 1353
Size: 188 676 3423
Fragments arranged by size:
3423 1288 676 488 188 149 96 90
79 73 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2705 5143 5517 5663 387
Size: 1064 138 1116 2438 374 146 1280
Fragments arranged by size:
2438 1280 1116 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2651 2651
Size: 6556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2688 2951 3120 3306 4619 5216 6003
Size: 393 1056 263 169 186 1313 597 787
Cuts at: 6003 6271 1239
Size: 268 1524
Fragments arranged by size:
1524 1313 1056 787 597 393 268 263
186 169
EcoNI CCTnn'n_nnAGG
Cuts at: 2161 2477 2161
Size: 316 6240
EcoO109I rG'GnC_Cy
Cuts at: 3008 3710 3008
Size: 702 5854
EcoRI G'AATT_C
Cuts at: 3865 4243 3865
Size: 378 6178
FspI TGC'GCA
Cuts at: 5062 5062
Size: 6556
HaeII r_GCGC'y
Cuts at: 1678 5703 1678
Size: 4025 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2129 2161 2801 2833 2974
Size: 32 135 32 557 32 640 32 141
Cuts at: 2974 3006 3462 3494 5197 5229 5271 5303 1373
Size: 32 456 32 1703 32 42 32 2626
Fragments arranged by size:
2626 1703 640 557 456 141 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2137 3535 3900 4146 669
Size: 988 281 199 1398 365 246 3079
Fragments arranged by size:
3079 1398 988 365 281 246 199
HindIII A'AGCT_T
Cuts at: 748 3187 748
Size: 2439 4117
HpaI GTT'AAC
Cuts at: 4146 4146
Size: 6556
KpnI G_GTAC'C
Cuts at: 3492 3881 3492
Size: 389 6167
MfeI C'AATT_G
Cuts at: 4155 4155
Size: 6556
MluI A'CGCG_T
Cuts at: 6432 6432
Size: 6556
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5670 5854 1305
Size: 4365 184 2007
Fragments arranged by size:
4365 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4734
Fragments arranged by size:
4734 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2348 2915 4514 4873 5032 518
Size: 648 1182 567 1599 359 159 2042
Fragments arranged by size:
2042 1599 1182 648 567 359 159
MspA1I CmG'CkG
Cuts at: 1606 3162 3584 3972 4649 5797 6042 1606
Size: 1556 422 388 677 1148 245 2120
Fragments arranged by size:
2120 1556 1148 677 422 388 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6004
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6556
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6556
NotI GC'GGCC_GC
Cuts at: 4003 4003
Size: 6556
NspI r_CATG'y
Cuts at: 1132 1229 2085 3914 5459 1132
Size: 97 856 1829 1545 2229
Fragments arranged by size:
2229 1829 1545 856 97
PasI CC'CwG_GG
Cuts at: 1348 2374 1348
Size: 1026 5530
PciI A'CATG_T
Cuts at: 2081 5455 2081
Size: 3374 3182
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6556
PfoI T'CCnGG_A
Cuts at: 3430 3430
Size: 6556
PmeI GTTT'AAAC
Cuts at: 3860 3860
Size: 6556
PmlI CAC'GTG
Cuts at: 2772 2772
Size: 6556
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4625 4657 6138 6170 4625
Size: 32 1481 32 5011
Fragments arranged by size:
5011 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6556
PsiI TTA'TAA
Cuts at: 4126 4126
Size: 6556
PspOMI G'GGCC_C
Cuts at: 3007 3007
Size: 6556
PstI C_TGCA'G
Cuts at: 830 2098 2783 3079 3093 3908 830
Size: 1268 685 296 14 815 3478
Fragments arranged by size:
3478 1268 815 685 296 14
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6556
PvuII CAG'CTG
Cuts at: 3584 3584
Size: 6556
SacI G_AGCT'C
Cuts at: 2653 2653
Size: 6556
SalI G'TCGA_C
Cuts at: 1936 3898 1936
Size: 1962 4594
SapI GCTCTTCn'nnn_
Cuts at: 2641 2641
Size: 6556
SbfI CC_TGCA'GG
Cuts at: 3908 3908
Size: 6556
ScaI AGT'ACT
Cuts at: 2612 4804 6516 2612
Size: 2192 1712 2652
Fragments arranged by size:
2652 2192 1712
SfcI C'TryA_G
Cuts at: 826 1045 2094 2779 3075 3089 3904 5039 5720
Size: 219 1049 685 296 14 815 1135 681
Cuts at: 5720 5911 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1049 815 685 681 296 219
191 14
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6556
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6556
SmaI CCC'GGG
Cuts at: 1872 3883 1872
Size: 2011 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3681 4660 5561 5823 820
Size: 197 926 1738 979 901 262 1553
Fragments arranged by size:
1738 1553 979 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4347 493
Size: 3854 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6556
SphI G_CATG'C
Cuts at: 1132 3914 1132
Size: 2782 3774
SspI AAT'ATT
Cuts at: 5 52 3032 4480 5
Size: 47 2980 1448 2081
Fragments arranged by size:
2980 2081 1448 47
StuI AGG'CCT
Cuts at: 1517 3560 1517
Size: 2043 4513
StyI C'CwwG_G
Cuts at: 513 1065 3115 3995 513
Size: 552 2050 880 3074
Fragments arranged by size:
3074 2050 880 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3311 4583 4600 4753 4938 5383 325
Size: 1255 1731 1272 17 153 185 445 1498
Fragments arranged by size:
1731 1498 1272 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2293 2316 2409
Size: 274 80 33 51 1443 316 23 93
Cuts at: 2409 2610 4802 6514 96
Size: 201 2192 1712 138
Fragments arranged by size:
2192 1712 1443 316 274 201 138 93
80 51 33 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6516 6548 6516
Size: 32 6524
Tth111I GACn'n_nGTC
Cuts at: 1316 2216 3476 1316
Size: 900 1260 4396
Fragments arranged by size:
4396 1260 900
XbaI T'CTAG_A
Cuts at: 3892 3892
Size: 6556
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2414 2414
Size: 6556
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6556
XmaI C'CCGG_G
Cuts at: 1870 3881 1870
Size: 2011 4545
XmnI GAAnn'nnTTC
Cuts at: 4685 4685
Size: 6556
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4364 276
Size: 53 83 186 936 2830 2468
Fragments arranged by size:
2830 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoRV FalI
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PpuMI PspXI PsrI RsrII SacII SanDI SexAI
SfiI SfoI SrfI SwaI