Restriction map for
pFN21AE3250
(Circular) (Six-base) MAPSORT of: pFN21AE3250.seq Check: 2882 from: 1 to: 6436
pFN21AE3250 6436 bp
With 182 enzymes: SgfI *
March 28, 2012 14:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4246 278
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3779 1937
Size: 1842 4594
Acc65I G'GTAC_C
Cuts at: 3757 3757
Size: 6436
AclI AA'CG_TT
Cuts at: 1563 4563 4936 1563
Size: 3000 373 3063
Fragments arranged by size:
3063 3000 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3346 4499 5883 6151 1632
Size: 1714 1153 1384 268 1917
Fragments arranged by size:
1917 1714 1384 1153 268
AflII C'TTAA_G
Cuts at: 820 1017 3446 820
Size: 197 2429 3810
Fragments arranged by size:
3810 2429 197
AflIII A'CryG_T
Cuts at: 1203 2459 5335 6312 1203
Size: 1256 2876 977 1327
Fragments arranged by size:
2876 1327 1256 977
AgeI A'CCGG_T
Cuts at: 5267 5267
Size: 6436
AhdI GACnn_n'nnGTC
Cuts at: 5165 5165
Size: 6436
AleI CACnn'nnGTG
Cuts at: 1166 2460 1166
Size: 1294 5142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6436
AlwNI CAG_nnn'CTG
Cuts at: 4179 5751 4179
Size: 1572 4864
ApaLI G'TGCA_C
Cuts at: 4493 5649 4493
Size: 1156 5280
ApoI r'AATT_y
Cuts at: 1493 2307 2918 3745 3971 4123 6232 1493
Size: 814 611 827 226 152 2109 1697
Fragments arranged by size:
2109 1697 827 814 611 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4990 160
Size: 872 3958 1606
Fragments arranged by size:
3958 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
AvaI C'yCGr_G
Cuts at: 1870 1943 2009 2020 3761 1870
Size: 73 66 11 1741 4545
Fragments arranged by size:
4545 1741 73 66 11
BamHI G'GATC_C
Cuts at: 3766 4133 3766
Size: 367 6069
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2524 3757 5212 618
Size: 325 217 627 737 1233 1455 1842
Fragments arranged by size:
1842 1455 1233 737 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3755 1391
Size: 2364 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4627 4661 1903
Size: 34 50 34 2606 34 3678
Fragments arranged by size:
3678 2606 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4328 5538 4328
Size: 1210 5226
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6436
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5047 136
Size: 107 122 71 631 605 3375 1525
Fragments arranged by size:
3375 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6431 6431
Size: 6436
BlpI GC'TnA_GC
Cuts at: 3853 3853
Size: 6436
Bme1580I G_kGCm'C
Cuts at: 1790 2990 4497 5653 1790
Size: 1200 1507 1156 2573
Fragments arranged by size:
2573 1507 1200 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6436
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2743 4167 5125
Size: 416 53 83 399 24 1326 1424 958
Cuts at: 5125 442
Size: 1753
Fragments arranged by size:
1753 1424 1326 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2252 2334 2920 2988 3072 3156 3240 3256
Size: 1013 82 586 68 84 84 84 16
Cuts at: 3256 3324 3340 3492 3576 5096 1239
Size: 68 16 152 84 1520 2579
Fragments arranged by size:
2579 1520 1013 586 152 84 84 84
84 82 68 68 16 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 4561 5426 5724 2419
Size: 2142 865 298 3131
Fragments arranged by size:
3131 2142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5099 882
Size: 617 3600 2219
Fragments arranged by size:
3600 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4227 493
Size: 711 3023 2702
Fragments arranged by size:
3023 2702 711
BsaBI GATnn'nnATC
Cuts at: 6430 6430
Size: 6436
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2655 4243 4625 5260
Size: 53 83 186 936 1122 1588 382 635
Cuts at: 5260 275
Size: 1451
Fragments arranged by size:
1588 1451 1122 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3288 4869 5267 5541 5688 6099 3288
Size: 1581 398 274 147 411 3625
Fragments arranged by size:
3625 1581 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2307 2337 1178
Size: 30 165 30 904 30 5277
Fragments arranged by size:
5277 904 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6436
BseYI C'CCAG_C
Cuts at: 5639 6135 5639
Size: 496 5940
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2839 3343 3511 3595 3679 1163
Size: 573 1103 504 168 84 84 3920
Fragments arranged by size:
3920 1103 573 504 168 84 84
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2073 3886 4647 5675 1540
Size: 275 186 72 1813 761 1028 2301
Fragments arranged by size:
2301 1813 1028 761 275 186 72
BsiHKAI G_wGCw'C
Cuts at: 4497 4582 5653 4497
Size: 85 1071 5280
Fragments arranged by size:
5280 1071 85
BsmI GAATG_Cn'
Cuts at: 2371 2906 3158 3947 4040 2371
Size: 535 252 789 93 4767
Fragments arranged by size:
4767 789 535 252 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2990 3755 4497 4582 5653 1391
Size: 399 1200 765 742 85 1071 2174
Fragments arranged by size:
2174 1200 1071 765 742 399 85
BspEI T'CCGG_A
Cuts at: 6099 6099
Size: 6436
BspHI T'CATG_A
Cuts at: 3232 4325 6055 3232
Size: 1093 1730 3613
Fragments arranged by size:
3613 1730 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2609 3791 844
Size: 1765 1182 3489
Fragments arranged by size:
3489 1765 1182
BsrBI CCG'CTC
Cuts at: 1394 1823 4323 1394
Size: 429 2500 3507
Fragments arranged by size:
3507 2500 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2911 3247 4931 5105 65
Size: 1183 1663 336 1684 174 1396
Fragments arranged by size:
1684 1663 1396 1183 336 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5080 5267 1700
Size: 110 3270 187 2869
Fragments arranged by size:
3270 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6436
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6436
BssSI C'ACGA_G
Cuts at: 4496 5508 4496
Size: 1012 5424
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6436
BstBI TT'CG_AA
Cuts at: 5240 5240
Size: 6436
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6436
BstYI r'GATC_y
Cuts at: 1972 2185 3766 4133 4518 4535 5987 6073 6085
Size: 213 1581 367 385 17 1452 86 12
Cuts at: 6085 6431 1972
Size: 346 1977
Fragments arranged by size:
1977 1581 1452 385 367 346 213 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5884
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3104 487
Size: 683 56 1878 3819
Fragments arranged by size:
3819 1878 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 3499 3583 3952 4746 4766 2547
Size: 952 84 369 794 20 4217
Fragments arranged by size:
4217 952 794 369 84 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6436
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2893 2928 3229 3264 529
Size: 35 2329 35 301 35 3701
Fragments arranged by size:
3701 2329 301 35 35 35
DraI TTT'AAA
Cuts at: 3740 3740
Size: 6436
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5443 809
Size: 507 4127 1802
Fragments arranged by size:
4127 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2157 2628 3883
Size: 54 1425 50 275 18 327 471 1255
Cuts at: 3883 4772 6401 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1255 889 471 327 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3883 1537
Size: 275 2071 4090
Fragments arranged by size:
4090 2071 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4366 1353
Size: 3013 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 5023 5397 5543 387
Size: 1064 138 450 2984 374 146 1280
Fragments arranged by size:
2984 1280 1064 450 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2252 2334 2920 2988 3072 3156 3240
Size: 393 620 82 586 68 84 84 84
Cuts at: 3240 3256 3324 3340 3346 3492 3576 4499 5096
Size: 16 68 16 6 146 84 923 597
Cuts at: 5096 5883 6151 1239
Size: 787 268 1524
Fragments arranged by size:
1524 923 787 620 597 586 393 268
146 84 84 84 84 82 68 68
16 16 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2195 2195
Size: 6436
EcoO109I rG'GnC_Cy
Cuts at: 2083 2083
Size: 6436
EcoRI G'AATT_C
Cuts at: 3745 4123 3745
Size: 378 6058
FspI TGC'GCA
Cuts at: 4942 4942
Size: 6436
HaeII r_GCGC'y
Cuts at: 1678 5583 1678
Size: 3905 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2841 2873 5077
Size: 32 135 32 965 32 272 32 2204
Cuts at: 5077 5109 5151 5183 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2204 965 272 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3780 4026 669
Size: 988 281 1842 246 3079
Fragments arranged by size:
3079 1842 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6436
HpaI GTT'AAC
Cuts at: 4026 4026
Size: 6436
KpnI G_GTAC'C
Cuts at: 3761 3761
Size: 6436
MfeI C'AATT_G
Cuts at: 4035 4035
Size: 6436
MluI A'CGCG_T
Cuts at: 6312 6312
Size: 6436
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2565 3129 3465 5550 5734 1305
Size: 1260 564 336 2085 184 2007
Fragments arranged by size:
2085 2007 1260 564 336 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2630 10
Size: 54 1425 343 798 3816
Fragments arranged by size:
3816 1425 798 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2460 4394 4753 4912 518
Size: 648 1294 1934 359 159 2042
Fragments arranged by size:
2042 1934 1294 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3852 4529 5677 5922 1606
Size: 2246 677 1148 245 2120
Fragments arranged by size:
2246 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5884
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6436
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6436
NotI GC'GGCC_GC
Cuts at: 3883 3883
Size: 6436
NspI r_CATG'y
Cuts at: 1132 1229 2463 2841 3794 5339 1132
Size: 97 1234 378 953 1545 2229
Fragments arranged by size:
2229 1545 1234 953 378 97
PasI CC'CwG_GG
Cuts at: 1348 2257 1348
Size: 909 5527
PciI A'CATG_T
Cuts at: 2459 5335 2459
Size: 2876 3560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6436
PmeI GTTT'AAAC
Cuts at: 3740 3740
Size: 6436
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4505 4537 6018 6050 4505
Size: 32 1481 32 4891
Fragments arranged by size:
4891 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2083 2083
Size: 6436
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6436
PsiI TTA'TAA
Cuts at: 4006 4006
Size: 6436
PstI C_TGCA'G
Cuts at: 830 2606 3788 830
Size: 1776 1182 3478
Fragments arranged by size:
3478 1776 1182
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6436
SalI G'TCGA_C
Cuts at: 1936 3778 1936
Size: 1842 4594
SanDI GG'GwC_CC
Cuts at: 2083 2083
Size: 6436
SbfI CC_TGCA'GG
Cuts at: 2606 3788 2606
Size: 1182 5254
ScaI AGT'ACT
Cuts at: 4684 6396 4684
Size: 1712 4724
SfcI C'TryA_G
Cuts at: 826 1045 2106 2602 3784 4919 5600 5791 826
Size: 219 1061 496 1182 1135 681 191 1471
Fragments arranged by size:
1471 1182 1135 1061 681 496 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6436
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6436
SmaI CCC'GGG
Cuts at: 1872 2022 3763 1872
Size: 150 1741 4545
Fragments arranged by size:
4545 1741 150
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2398 3446 4540 5441 5703 820
Size: 197 926 455 1048 1094 901 262 1553
Fragments arranged by size:
1553 1094 1048 926 901 455 262 197
SnaBI TAC'GTA
Cuts at: 493 4227 493
Size: 3734 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6436
SphI G_CATG'C
Cuts at: 1132 3794 1132
Size: 2662 3774
SspI AAT'ATT
Cuts at: 5 52 4360 5
Size: 47 4308 2081
Fragments arranged by size:
4308 2081 47
StuI AGG'CCT
Cuts at: 1517 2893 2977 3061 3145 3397 3565 3649 1517
Size: 1376 84 84 84 252 168 84 4304
Fragments arranged by size:
4304 1376 252 168 84 84 84 84
StyI C'CwwG_G
Cuts at: 513 1065 2428 3875 513
Size: 552 1363 1447 3074
Fragments arranged by size:
3074 1447 1363 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4463 4480 4633 4818 5263 325
Size: 1255 2883 17 153 185 445 1498
Fragments arranged by size:
2883 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2163 2214 4682
Size: 274 80 33 51 1443 186 51 2468
Cuts at: 4682 6394 96
Size: 1712 138
Fragments arranged by size:
2468 1712 1443 274 186 138 80 51
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2307 2339 6396 6428 2307
Size: 32 4057 32 2315
Fragments arranged by size:
4057 2315 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6436
XbaI T'CTAG_A
Cuts at: 3772 3772
Size: 6436
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6436
XmaI C'CCGG_G
Cuts at: 1870 2020 3761 1870
Size: 150 1741 4545
Fragments arranged by size:
4545 1741 150
XmnI GAAnn'nnTTC
Cuts at: 2621 4565 2621
Size: 1944 4492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4244 276
Size: 53 83 186 936 2710 2468
Fragments arranged by size:
2710 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI
SexAI SfiI SfoI SrfI SwaI XcmI