Restriction map for
pFN21AE3242
(Circular) (Six-base) MAPSORT of: pFN21AE3242.seq Check: 6174 from: 1 to: 6400
pFN21AE3242 6400 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4210 278
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3444 3507 3597 3743 1937
Size: 1507 63 90 146 4594
Fragments arranged by size:
4594 1507 146 90 63
Acc65I G'GTAC_C
Cuts at: 3721 3721
Size: 6400
AclI AA'CG_TT
Cuts at: 1563 3085 4527 4900 1563
Size: 1522 1442 373 3063
Fragments arranged by size:
3063 1522 1442 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2802 2942 4463 5847 6115 1632
Size: 1170 140 1521 1384 268 1917
Fragments arranged by size:
1917 1521 1384 1170 268 140
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6203
AflIII A'CryG_T
Cuts at: 1203 2661 5299 6276 1203
Size: 1458 2638 977 1327
Fragments arranged by size:
2638 1458 1327 977
AgeI A'CCGG_T
Cuts at: 5231 5231
Size: 6400
AhdI GACnn_n'nnGTC
Cuts at: 5129 5129
Size: 6400
AleI CACnn'nnGTG
Cuts at: 1166 3691 1166
Size: 2525 3875
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6400
AlwNI CAG_nnn'CTG
Cuts at: 2132 2692 3463 4143 5715 2132
Size: 560 771 680 1572 2817
Fragments arranged by size:
2817 1572 771 680 560
ApaI G_GGCC'C
Cuts at: 2104 2104
Size: 6400
ApaLI G'TGCA_C
Cuts at: 4457 5613 4457
Size: 1156 5244
ApoI r'AATT_y
Cuts at: 1493 3709 3935 4087 6196 1493
Size: 2216 226 152 2109 1697
Fragments arranged by size:
2216 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4954 160
Size: 872 3922 1606
Fragments arranged by size:
3922 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
AvaI C'yCGr_G
Cuts at: 1870 1943 2040 2067 3411 3725 1870
Size: 73 97 27 1344 314 4545
Fragments arranged by size:
4545 1344 314 97 73 27
AvrII C'CTAG_G
Cuts at: 2482 3469 2482
Size: 987 5413
BamHI G'GATC_C
Cuts at: 2075 3730 4097 2075
Size: 1655 367 4378
Fragments arranged by size:
4378 1655 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 2396 2749 2804 2814
Size: 325 217 627 601 8 353 55 10
Cuts at: 2814 3576 3693 3721 5176 618
Size: 762 117 28 1455 1842
Fragments arranged by size:
1842 1455 762 627 601 353 325 217
117 55 28 10 8
BanII G_rGCy'C
Cuts at: 1391 2104 2532 2630 2770 2778 2977 3031 3719
Size: 713 428 98 140 8 199 54 688
Cuts at: 3719 1391
Size: 4072
Fragments arranged by size:
4072 713 688 428 199 140 98 54
8
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2854 928
Size: 971 955 4474
Fragments arranged by size:
4474 971 955
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3317 3351 4591 4625 1903
Size: 34 50 34 1296 34 1240 34 3678
Fragments arranged by size:
3678 1296 1240 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2885 4292 5502 2885
Size: 1407 1210 3783
Fragments arranged by size:
3783 1407 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6400
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2099 2167 2487
Size: 107 122 71 631 605 427 68 320
Cuts at: 2487 5011 136
Size: 2524 1525
Fragments arranged by size:
2524 1525 631 605 427 320 122 107
71 68
BglII A'GATC_T
Cuts at: 6395 6395
Size: 6400
BlpI GC'TnA_GC
Cuts at: 3817 3817
Size: 6400
Bme1580I G_kGCm'C
Cuts at: 1790 2104 2391 2809 4461 5617 1790
Size: 314 287 418 1652 1156 2573
Fragments arranged by size:
2573 1652 1156 418 314 287
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6400
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3302 4131 5089
Size: 416 53 83 399 24 1885 829 958
Cuts at: 5089 442
Size: 1753
Fragments arranged by size:
1885 1753 958 829 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6400
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2718 2718
Size: 6400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2222 2775 3113 5060 1239
Size: 983 553 338 1947 2579
Fragments arranged by size:
2579 1947 983 553 338
Bpu10I CC'TnA_GC
Cuts at: 2442 2442
Size: 6400
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2177 3134 4525 5390 5688 2177
Size: 957 1391 865 298 2889
Fragments arranged by size:
2889 1391 957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5063 882
Size: 617 3564 2219
Fragments arranged by size:
3564 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2664 3658 4191 493
Size: 711 1460 994 533 2702
Fragments arranged by size:
2702 1460 994 711 533
BsaBI GATnn'nnATC
Cuts at: 6394 6394
Size: 6400
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4207 4589 5224 275
Size: 53 83 186 936 2674 382 635 1451
Fragments arranged by size:
2674 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2399 4833 5231 5505 5652 6063 2399
Size: 2434 398 274 147 411 2736
Fragments arranged by size:
2736 2434 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2807 2837 1178
Size: 30 165 30 1404 30 4741
Fragments arranged by size:
4741 1404 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2772 2918 3036 1186
Size: 1586 146 118 4550
Fragments arranged by size:
4550 1586 146 118
BseYI C'CCAG_C
Cuts at: 2125 2206 3233 5603 6099 2125
Size: 81 1027 2370 496 2426
Fragments arranged by size:
2426 2370 1027 496 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2400 1163
Size: 573 664 5163
Fragments arranged by size:
5163 664 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3850 4611 5639 1540
Size: 275 186 1849 761 1028 2301
Fragments arranged by size:
2301 1849 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2025 2778 3387 4461 4546 5617 2025
Size: 753 609 1074 85 1071 2808
Fragments arranged by size:
2808 1074 1071 753 609 85
BsmI GAATG_Cn'
Cuts at: 2680 3307 3911 4004 2680
Size: 627 604 93 5076
Fragments arranged by size:
5076 627 604 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2452 2452
Size: 6400
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2025 2104 2391 2532 2630 2770 2778
Size: 399 235 79 287 141 98 140 8
Cuts at: 2778 2809 2977 3031 3387 3719 4461 4546 5617
Size: 31 168 54 356 332 742 85 1071
Cuts at: 5617 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 356 332 287 235
168 141 140 98 85 79 54 31
8
BspEI T'CCGG_A
Cuts at: 6063 6063
Size: 6400
BspHI T'CATG_A
Cuts at: 4289 6019 4289
Size: 1730 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3755 844
Size: 2911 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2938 4287 1394
Size: 429 1115 1349 3507
Fragments arranged by size:
3507 1349 1115 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4895 5069 65
Size: 1183 3647 174 1396
Fragments arranged by size:
3647 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3573 5044 5231 1700
Size: 110 1763 1471 187 2869
Fragments arranged by size:
2869 1763 1471 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6400
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6400
BssSI C'ACGA_G
Cuts at: 3380 3609 4460 5472 3380
Size: 229 851 1012 4308
Fragments arranged by size:
4308 1012 851 229
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2749 794
Size: 1955 4445
BstBI TT'CG_AA
Cuts at: 2647 5204 2647
Size: 2557 3843
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6400
BstXI CCAn_nnnn'nTGG
Cuts at: 3164 3164
Size: 6400
BstYI r'GATC_y
Cuts at: 1972 2075 3730 4097 4482 4499 5951 6037 6049
Size: 103 1655 367 385 17 1452 86 12
Cuts at: 6049 6395 1972
Size: 346 1977
Fragments arranged by size:
1977 1655 1452 385 367 346 103 86
17 12
BstZ17I GTA'TAC
Cuts at: 3508 3508
Size: 6400
Bsu36I CC'TnA_GG
Cuts at: 2224 2224
Size: 6400
BtgI C'CryG_G
Cuts at: 513 1065 2392 2542 513
Size: 552 1327 150 4371
Fragments arranged by size:
4371 1327 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5661
Fragments arranged by size:
5661 683 56
BtsI GCAGTG_nn'
Cuts at: 2356 2693 3452 3916 4710 4730 2356
Size: 337 759 464 794 20 4026
Fragments arranged by size:
4026 794 759 464 337 20
ClaI AT'CG_AT
Cuts at: 1554 3397 1554
Size: 1843 4557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2436 2471 529
Size: 35 1872 35 4458
Fragments arranged by size:
4458 1872 35 35
DraI TTT'AAA
Cuts at: 3704 3704
Size: 6400
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3611 5407 809
Size: 507 2295 1796 1802
Fragments arranged by size:
2295 1802 1796 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2168 3553 3847
Size: 54 1425 50 275 18 338 1385 294
Cuts at: 3847 4736 6365 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1385 889 338 294 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3847 1537
Size: 275 2035 4090
Fragments arranged by size:
4090 2035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2715 3279 4330 1353
Size: 1362 564 1051 3423
Fragments arranged by size:
3423 1362 1051 564
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2465 2554 4987 5361 5507 387
Size: 1064 138 876 89 2433 374 146 1280
Fragments arranged by size:
2433 1280 1064 876 374 146 138 89
EcoICRI GAG'CTC
Cuts at: 2776 2776
Size: 6400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2775 2802 2942 3113 4463 5060
Size: 393 590 553 27 140 171 1350 597
Cuts at: 5060 5847 6115 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1350 787 597 590 553 393 268
171 140 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2757 2757
Size: 6400
EcoO109I rG'GnC_Cy
Cuts at: 2100 2228 2243 2100
Size: 128 15 6257
Fragments arranged by size:
6257 128 15
EcoRI G'AATT_C
Cuts at: 3709 4087 3709
Size: 378 6022
EcoRV GAT'ATC
Cuts at: 2562 2562
Size: 6400
FspI TGC'GCA
Cuts at: 4906 4906
Size: 6400
HaeII r_GCGC'y
Cuts at: 1678 5547 1678
Size: 3869 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2593 2625 3278 3310 3405
Size: 32 135 32 1021 32 653 32 95
Cuts at: 3405 3437 5041 5073 5115 5147 1373
Size: 32 1604 32 42 32 2626
Fragments arranged by size:
2626 1604 1021 653 135 95 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3744 3990 669
Size: 988 281 1416 390 246 3079
Fragments arranged by size:
3079 1416 988 390 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6400
HpaI GTT'AAC
Cuts at: 3990 3990
Size: 6400
KpnI G_GTAC'C
Cuts at: 3725 3725
Size: 6400
MfeI C'AATT_G
Cuts at: 3999 3999
Size: 6400
MluI A'CGCG_T
Cuts at: 6276 6276
Size: 6400
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3276 5514 5698 1305
Size: 1971 2238 184 2007
Fragments arranged by size:
2238 2007 1971 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3555 10
Size: 54 1425 343 1723 2855
Fragments arranged by size:
2855 1723 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3691 4358 4717 4876 518
Size: 648 2525 667 359 159 2042
Fragments arranged by size:
2525 2042 667 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3514 3816 4493 5641 5886 1606
Size: 1908 302 677 1148 245 2120
Fragments arranged by size:
2120 1908 1148 677 302 245
NcoI C'CATG_G
Cuts at: 513 1065 2542 513
Size: 552 1477 4371
Fragments arranged by size:
4371 1477 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6400
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6400
NotI GC'GGCC_GC
Cuts at: 3847 3847
Size: 6400
NspI r_CATG'y
Cuts at: 1132 1229 3596 3758 5303 1132
Size: 97 2367 162 1545 2229
Fragments arranged by size:
2367 2229 1545 162 97
PasI CC'CwG_GG
Cuts at: 1348 2097 1348
Size: 749 5651
PciI A'CATG_T
Cuts at: 5299 5299
Size: 6400
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2132 1108
Size: 1024 5376
PfoI T'CCnGG_A
Cuts at: 2494 2494
Size: 6400
PmeI GTTT'AAAC
Cuts at: 3704 3704
Size: 6400
PmlI CAC'GTG
Cuts at: 2664 2664
Size: 6400
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4469 4501 5982 6014 4469
Size: 32 1481 32 4855
Fragments arranged by size:
4855 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2361 2721 3321 1613
Size: 748 360 600 4692
Fragments arranged by size:
4692 748 600 360
PsiI TTA'TAA
Cuts at: 3970 3970
Size: 6400
PspOMI G'GGCC_C
Cuts at: 2100 2100
Size: 6400
PstI C_TGCA'G
Cuts at: 830 2688 3459 3752 830
Size: 1858 771 293 3478
Fragments arranged by size:
3478 1858 771 293
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6400
PvuII CAG'CTG
Cuts at: 3514 3514
Size: 6400
SacI G_AGCT'C
Cuts at: 2778 2778
Size: 6400
SalI G'TCGA_C
Cuts at: 1936 3742 1936
Size: 1806 4594
SapI GCTCTTCn'nnn_
Cuts at: 3279 3279
Size: 6400
SbfI CC_TGCA'GG
Cuts at: 3752 3752
Size: 6400
ScaI AGT'ACT
Cuts at: 4648 6360 4648
Size: 1712 4688
SexAI A'CCwGG_T
Cuts at: 3633 3633
Size: 6400
SfcI C'TryA_G
Cuts at: 826 1045 2684 3455 3748 4883 5564 5755 826
Size: 219 1639 771 293 1135 681 191 1471
Fragments arranged by size:
1639 1471 1135 771 681 293 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6400
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6400
SmaI CCC'GGG
Cuts at: 1872 3727 1872
Size: 1855 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2192 3149 4504 5405 5667 820
Size: 197 926 249 957 1355 901 262 1553
Fragments arranged by size:
1553 1355 957 926 901 262 249 197
SnaBI TAC'GTA
Cuts at: 493 4191 493
Size: 3698 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6400
SphI G_CATG'C
Cuts at: 1132 3758 1132
Size: 2626 3774
SspI AAT'ATT
Cuts at: 5 52 4324 5
Size: 47 4272 2081
Fragments arranged by size:
4272 2081 47
StuI AGG'CCT
Cuts at: 1517 2015 3161 1517
Size: 498 1146 4756
Fragments arranged by size:
4756 1146 498
StyI C'CwwG_G
Cuts at: 513 1065 2379 2482 2542 3469 3839 513
Size: 552 1314 103 60 927 370 3074
Fragments arranged by size:
3074 1314 927 552 370 103 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2464 3078 3333 4427 4444 4597 4782
Size: 1255 884 614 255 1094 17 153 185
Cuts at: 4782 5227 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1094 884 614 445 255 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3358 4646 6358
Size: 274 80 33 51 1443 1381 1288 1712
Cuts at: 6358 96
Size: 138
Fragments arranged by size:
1712 1443 1381 1288 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2546 2578 2807 2839 6360 6392 2546
Size: 32 229 32 3521 32 2554
Fragments arranged by size:
3521 2554 229 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6400
XbaI T'CTAG_A
Cuts at: 3736 3736
Size: 6400
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6400
XmaI C'CCGG_G
Cuts at: 1870 3725 1870
Size: 1855 4545
XmnI GAAnn'nnTTC
Cuts at: 4529 4529
Size: 6400
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4208 276
Size: 53 83 186 936 2674 2468
Fragments arranged by size:
2674 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI
BsiWI DraIII FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PpuMI PspXI PsrI RsrII
SacII SanDI SfiI SfoI SrfI SwaI XcmI