Restriction map for
pFN21AE3240
(Circular) (Six-base) MAPSORT of: pFN21AE3240.seq Check: 7120 from: 1 to: 6391
pFN21AE3240 6391 bp
With 182 enzymes: SgfI *
March 28, 2012 12:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4201 278
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3734 1937
Size: 1797 4594
Acc65I G'GTAC_C
Cuts at: 3712 3712
Size: 6391
AclI AA'CG_TT
Cuts at: 1563 4518 4891 1563
Size: 2955 373 3063
Fragments arranged by size:
3063 2955 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2789 3356 4454 5838 6106 1632
Size: 1157 567 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1157 1098 567 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6194
AflIII A'CryG_T
Cuts at: 1203 5290 6267 1203
Size: 4087 977 1327
Fragments arranged by size:
4087 1327 977
AgeI A'CCGG_T
Cuts at: 5222 5222
Size: 6391
AhdI GACnn_n'nnGTC
Cuts at: 5120 5120
Size: 6391
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6391
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2865 1184
Size: 1681 4710
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2909 2941 2909
Size: 32 6359
AlwNI CAG_nnn'CTG
Cuts at: 3047 3260 4134 5706 3047
Size: 213 874 1572 3732
Fragments arranged by size:
3732 1572 874 213
ApaLI G'TGCA_C
Cuts at: 4448 5604 4448
Size: 1156 5235
ApoI r'AATT_y
Cuts at: 1493 3700 3926 4078 6187 1493
Size: 2207 226 152 2109 1697
Fragments arranged by size:
2207 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4945 160
Size: 872 3913 1606
Fragments arranged by size:
3913 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
AvaI C'yCGr_G
Cuts at: 1870 1943 3359 3396 3716 1870
Size: 73 1416 37 320 4545
Fragments arranged by size:
4545 1416 320 73 37
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2166 2199 2166
Size: 33 6358
BamHI G'GATC_C
Cuts at: 3721 4088 3721
Size: 367 6024
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3307 3712 5167 618
Size: 325 217 627 1520 405 1455 1842
Fragments arranged by size:
1842 1520 1455 627 405 325 217
BanII G_rGCy'C
Cuts at: 1391 2886 3234 3333 3710 1391
Size: 1495 348 99 377 4072
Fragments arranged by size:
4072 1495 377 348 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2204 928
Size: 971 305 5115
Fragments arranged by size:
5115 971 305
BbvCI CC'TCA_GC
Cuts at: 2052 2052
Size: 6391
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4582 4616 1903
Size: 34 2645 34 3678
Fragments arranged by size:
3678 2645 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2167 4283 5493 2167
Size: 2116 1210 3065
Fragments arranged by size:
3065 2116 1210
BclI T'GATC_A
Cuts at: 1548 3276 1548
Size: 1728 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3350 5002 136
Size: 107 122 71 631 605 1678 1652 1525
Fragments arranged by size:
1678 1652 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6386 6386
Size: 6391
BlpI GC'TnA_GC
Cuts at: 3808 3808
Size: 6391
Bme1580I G_kGCm'C
Cuts at: 1790 3312 4452 5608 1790
Size: 1522 1140 1156 2573
Fragments arranged by size:
2573 1522 1156 1140
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6391
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2156 4122 5080
Size: 416 53 83 399 24 739 1966 958
Cuts at: 5080 442
Size: 1753
Fragments arranged by size:
1966 1753 958 739 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6391
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2592 3045 5051 1239
Size: 1353 453 2006 2579
Fragments arranged by size:
2579 2006 1353 453
Bpu10I CC'TnA_GC
Cuts at: 2052 2052
Size: 6391
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4516 5381 5679 4516
Size: 865 298 5228
Fragments arranged by size:
5228 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3294 3432 5054 882
Size: 617 1795 138 1622 2219
Fragments arranged by size:
2219 1795 1622 617 138
BsaAI yAC'GTr
Cuts at: 493 1204 4182 493
Size: 711 2978 2702
Fragments arranged by size:
2978 2702 711
BsaBI GATnn'nnATC
Cuts at: 6385 6385
Size: 6391
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4198 4580 5215 275
Size: 53 83 186 936 2665 382 635 1451
Fragments arranged by size:
2665 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4824 5222 5496 5643 6054 4824
Size: 398 274 147 411 5161
Fragments arranged by size:
5161 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2191 2221 3291 3321 1178
Size: 30 165 30 788 30 1070 30 4248
Fragments arranged by size:
4248 1070 788 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2378 1186
Size: 1192 5199
BseYI C'CCAG_C
Cuts at: 5594 6090 5594
Size: 496 5895
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2796 3652 1163
Size: 573 1060 856 3902
Fragments arranged by size:
3902 1060 856 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2908 3841 4602 5630 1540
Size: 275 186 907 933 761 1028 2301
Fragments arranged by size:
2301 1028 933 907 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3234 3333 4452 4537 5608 3234
Size: 99 1119 85 1071 4017
Fragments arranged by size:
4017 1119 1071 99 85
BsmI GAATG_Cn'
Cuts at: 2851 3902 3995 2851
Size: 1051 93 5247
Fragments arranged by size:
5247 1051 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2886 3234 3312 3333 3710 4452 4537
Size: 399 1096 348 78 21 377 742 85
Cuts at: 4537 5608 1391
Size: 1071 2174
Fragments arranged by size:
2174 1096 1071 742 399 377 348 85
78 21
BspEI T'CCGG_A
Cuts at: 6054 6054
Size: 6391
BspHI T'CATG_A
Cuts at: 2889 4280 6010 2889
Size: 1391 1730 3270
Fragments arranged by size:
3270 1730 1391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2975 3746 844
Size: 2131 771 3489
Fragments arranged by size:
3489 2131 771
BsrBI CCG'CTC
Cuts at: 1394 1823 4278 1394
Size: 429 2455 3507
Fragments arranged by size:
3507 2455 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4886 5060 65
Size: 1183 3638 174 1396
Fragments arranged by size:
3638 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2292 5035 5222 1700
Size: 110 482 2743 187 2869
Fragments arranged by size:
2869 2743 482 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6391
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6391
BssSI C'ACGA_G
Cuts at: 4451 5463 4451
Size: 1012 5379
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6391
BstBI TT'CG_AA
Cuts at: 5195 5195
Size: 6391
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6391
BstYI r'GATC_y
Cuts at: 1972 2101 2234 2335 2514 3044 3721 4088 4473
Size: 129 133 101 179 530 677 367 385
Cuts at: 4473 4490 5942 6028 6040 6386 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 677 530 385 367 346 179
133 129 101 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3313 3313
Size: 6391
BtgI C'CryG_G
Cuts at: 513 1065 3345 513
Size: 552 2280 3559
Fragments arranged by size:
3559 2280 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2925 3529 487
Size: 683 56 1699 604 3349
Fragments arranged by size:
3349 1699 683 604 56
BtsI GCAGTG_nn'
Cuts at: 3907 4701 4721 3907
Size: 794 20 5577
Fragments arranged by size:
5577 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6391
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6356
DraI TTT'AAA
Cuts at: 3695 3695
Size: 6391
DraIII CAC_nnn'GTG
Cuts at: 3180 3182 3180
Size: 2 6389
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5398 809
Size: 507 4082 1802
Fragments arranged by size:
4082 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3351 3838 4727
Size: 54 1425 50 275 18 1521 487 889
Cuts at: 4727 6356 8
Size: 1629 43
Fragments arranged by size:
1629 1521 1425 889 487 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3838 1537
Size: 275 2026 4090
Fragments arranged by size:
4090 2026 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 3411 4321 1353
Size: 1281 777 910 3423
Fragments arranged by size:
3423 1281 910 777
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4978 5352 5498 387
Size: 1064 138 3389 374 146 1280
Fragments arranged by size:
3389 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3232 3331 3232
Size: 99 6292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2592 2789 3045 3356 4454 5051 5838
Size: 393 960 197 256 311 1098 597 787
Cuts at: 5838 6106 1239
Size: 268 1524
Fragments arranged by size:
1524 1098 960 787 597 393 311 268
256 197
EcoNI CCTnn'n_nnAGG
Cuts at: 3239 3338 3239
Size: 99 6292
EcoRI G'AATT_C
Cuts at: 3700 4078 3700
Size: 378 6013
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2218 2218
Size: 6391
FspI TGC'GCA
Cuts at: 4897 4897
Size: 6391
HaeII r_GCGC'y
Cuts at: 1678 3556 5538 1678
Size: 1878 1982 2531
Fragments arranged by size:
2531 1982 1878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2191 2223 2388 2420 3029
Size: 32 135 32 619 32 165 32 609
Cuts at: 3029 3061 3564 3596 5032 5064 5106 5138 1373
Size: 32 503 32 1436 32 42 32 2626
Fragments arranged by size:
2626 1436 619 609 503 165 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2950 3735 3981 669
Size: 988 281 1012 785 246 3079
Fragments arranged by size:
3079 1012 988 785 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6391
HpaI GTT'AAC
Cuts at: 3981 3981
Size: 6391
KpnI G_GTAC'C
Cuts at: 3716 3716
Size: 6391
MfeI C'AATT_G
Cuts at: 3990 3990
Size: 6391
MluI A'CGCG_T
Cuts at: 6267 6267
Size: 6391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5505 5689 1305
Size: 4200 184 2007
Fragments arranged by size:
4200 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3353 10
Size: 54 1425 343 1521 3048
Fragments arranged by size:
3048 1521 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2489 2939 3584 4349 4708 4867 518
Size: 648 1323 450 645 765 359 159 2042
Fragments arranged by size:
2042 1323 765 648 645 450 359 159
MspA1I CmG'CkG
Cuts at: 1606 3807 4484 5632 5877 1606
Size: 2201 677 1148 245 2120
Fragments arranged by size:
2201 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3345 513
Size: 552 2280 3559
Fragments arranged by size:
3559 2280 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6391
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6391
NotI GC'GGCC_GC
Cuts at: 3838 3838
Size: 6391
NspI r_CATG'y
Cuts at: 1132 1229 3749 5294 1132
Size: 97 2520 1545 2229
Fragments arranged by size:
2520 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6391
PciI A'CATG_T
Cuts at: 5290 5290
Size: 6391
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2216 2432 1108
Size: 1108 216 5067
Fragments arranged by size:
5067 1108 216
PfoI T'CCnGG_A
Cuts at: 2425 2871 2425
Size: 446 5945
PmeI GTTT'AAAC
Cuts at: 3695 3695
Size: 6391
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4460 4492 5973 6005 4460
Size: 32 1481 32 4846
Fragments arranged by size:
4846 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6391
PsiI TTA'TAA
Cuts at: 3961 3961
Size: 6391
PstI C_TGCA'G
Cuts at: 830 2353 2983 3743 830
Size: 1523 630 760 3478
Fragments arranged by size:
3478 1523 760 630
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6391
SacI G_AGCT'C
Cuts at: 3234 3333 3234
Size: 99 6292
SalI G'TCGA_C
Cuts at: 1936 3733 1936
Size: 1797 4594
SbfI CC_TGCA'GG
Cuts at: 3743 3743
Size: 6391
ScaI AGT'ACT
Cuts at: 4639 6351 4639
Size: 1712 4679
SexAI A'CCwGG_T
Cuts at: 3303 3303
Size: 6391
SfcI C'TryA_G
Cuts at: 826 1045 2349 2979 3739 4874 5555 5746 826
Size: 219 1304 630 760 1135 681 191 1471
Fragments arranged by size:
1471 1304 1135 760 681 630 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 3350 3350
Size: 6391
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6391
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6391
SmaI CCC'GGG
Cuts at: 1872 3718 1872
Size: 1846 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4495 5396 5658 820
Size: 197 926 2552 901 262 1553
Fragments arranged by size:
2552 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4182 493
Size: 3689 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6391
SphI G_CATG'C
Cuts at: 1132 3749 1132
Size: 2617 3774
SspI AAT'ATT
Cuts at: 5 52 4315 5
Size: 47 4263 2081
Fragments arranged by size:
4263 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6391
StyI C'CwwG_G
Cuts at: 513 1065 2022 3345 3830 513
Size: 552 957 1323 485 3074
Fragments arranged by size:
3074 1323 957 552 485
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2138 4418 4435 4588 4773 5218 325
Size: 1255 558 2280 17 153 185 445 1498
Fragments arranged by size:
2280 1498 1255 558 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4637 6349 96
Size: 274 80 33 51 1443 2660 1712 138
Fragments arranged by size:
2660 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3291 3323 6351 6383 3291
Size: 32 3028 32 3299
Fragments arranged by size:
3299 3028 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6391
XbaI T'CTAG_A
Cuts at: 3727 3727
Size: 6391
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2029 2029
Size: 6391
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6391
XmaI C'CCGG_G
Cuts at: 1870 3716 1870
Size: 1846 4545
XmnI GAAnn'nnTTC
Cuts at: 4520 4520
Size: 6391
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4199 276
Size: 53 83 186 936 2665 2468
Fragments arranged by size:
2665 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SfoI SrfI SwaI