Restriction map for
pFN21AE3239
(Circular) (Six-base) MAPSORT of: pFN21AE3239.seq Check: 6188 from: 1 to: 6382
pFN21AE3239 6382 bp
With 182 enzymes: SgfI *
March 28, 2012 12:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4192 278
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2858 3725 1937
Size: 921 867 4594
Fragments arranged by size:
4594 921 867
Acc65I G'GTAC_C
Cuts at: 3703 3703
Size: 6382
AclI AA'CG_TT
Cuts at: 1563 4509 4882 1563
Size: 2946 373 3063
Fragments arranged by size:
3063 2946 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2595 2646 3228 3427 4445 5829 6097 1632
Size: 963 51 582 199 1018 1384 268 1917
Fragments arranged by size:
1917 1384 1018 963 582 268 199 51
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6185
AflIII A'CryG_T
Cuts at: 1203 2897 3144 5281 6258 1203
Size: 1694 247 2137 977 1327
Fragments arranged by size:
2137 1694 1327 977 247
AgeI A'CCGG_T
Cuts at: 5213 5213
Size: 6382
AhdI GACnn_n'nnGTC
Cuts at: 5111 5111
Size: 6382
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6382
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6382
AlwNI CAG_nnn'CTG
Cuts at: 4125 5697 4125
Size: 1572 4810
ApaI G_GGCC'C
Cuts at: 2022 2022
Size: 6382
ApaLI G'TGCA_C
Cuts at: 4439 5595 4439
Size: 1156 5226
ApoI r'AATT_y
Cuts at: 1493 2885 3332 3691 3917 4069 6178 1493
Size: 1392 447 359 226 152 2109 1697
Fragments arranged by size:
2109 1697 1392 447 359 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4936 160
Size: 872 3904 1606
Fragments arranged by size:
3904 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
AvaI C'yCGr_G
Cuts at: 1870 1943 2123 2257 2297 2525 3707 1870
Size: 73 180 134 40 228 1182 4545
Fragments arranged by size:
4545 1182 228 180 134 73 40
BamHI G'GATC_C
Cuts at: 3712 4079 3712
Size: 367 6015
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 2510 3642 3703 5158
Size: 325 217 627 390 333 1132 61 1455
Cuts at: 5158 618
Size: 1842
Fragments arranged by size:
1842 1455 1132 627 390 333 325 217
61
BanII G_rGCy'C
Cuts at: 1391 2022 2233 3349 3701 1391
Size: 631 211 1116 352 4072
Fragments arranged by size:
4072 1116 631 352 211
BbeI G_GCGC'C
Cuts at: 2181 2514 2181
Size: 333 6049
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2509 2643 928
Size: 971 610 134 4667
Fragments arranged by size:
4667 971 610 134
BbvCI CC'TCA_GC
Cuts at: 2061 2492 2061
Size: 431 5951
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4573 4607 1903
Size: 34 2636 34 3678
Fragments arranged by size:
3678 2636 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4274 5484 4274
Size: 1210 5172
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6382
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2175 2334 2355
Size: 107 122 71 631 605 503 159 21
Cuts at: 2355 4993 136
Size: 2638 1525
Fragments arranged by size:
2638 1525 631 605 503 159 122 107
71 21
BglII A'GATC_T
Cuts at: 6377 6377
Size: 6382
BlpI GC'TnA_GC
Cuts at: 3799 3799
Size: 6382
Bme1580I G_kGCm'C
Cuts at: 1790 2022 4443 5599 1790
Size: 232 2421 1156 2573
Fragments arranged by size:
2573 2421 1156 232
BmgBI CAC'GTC
Cuts at: 1318 3246 1318
Size: 1928 4454
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4113 5071 442
Size: 416 53 83 399 24 2696 958 1753
Fragments arranged by size:
2696 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2052 2247 3333 5042 1239
Size: 813 195 1086 1709 2579
Fragments arranged by size:
2579 1709 1086 813 195
Bpu10I CC'TnA_GC
Cuts at: 2061 2492 2061
Size: 431 5951
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3277 4507 5372 5670 3277
Size: 1230 865 298 3989
Fragments arranged by size:
3989 1230 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5045 882
Size: 617 3546 2219
Fragments arranged by size:
3546 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4173 493
Size: 711 2969 2702
Fragments arranged by size:
2969 2702 711
BsaBI GATnn'nnATC
Cuts at: 6376 6376
Size: 6382
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2072 2178 2511 4189
Size: 53 83 186 936 539 106 333 1678
Cuts at: 4189 4571 5206 275
Size: 382 635 1451
Fragments arranged by size:
1678 1451 936 635 539 382 333 186
106 83 53
BsaWI w'CCGG_w
Cuts at: 2207 2233 4815 5213 5487 5634 6045 2207
Size: 26 2582 398 274 147 411 2544
Fragments arranged by size:
2582 2544 411 398 274 147 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3336 3366 1178
Size: 30 165 30 1933 30 4194
Fragments arranged by size:
4194 1933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 3326 1186
Size: 892 1248 4242
Fragments arranged by size:
4242 1248 892
BseYI C'CCAG_C
Cuts at: 2766 3270 5585 6081 2766
Size: 504 2315 496 3067
Fragments arranged by size:
3067 2315 504 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3242 3535 1163
Size: 573 1506 293 4010
Fragments arranged by size:
4010 1506 573 293
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3832 4593 5621 1540
Size: 275 186 48 1783 761 1028 2301
Fragments arranged by size:
2301 1783 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 2233 3293 3349 4443 4528 5599 2233
Size: 1060 56 1094 85 1071 3016
Fragments arranged by size:
3016 1094 1071 1060 85 56
BsmI GAATG_Cn'
Cuts at: 3893 3986 3893
Size: 93 6289
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2022 2233 3293 3349 3701 4443 4528
Size: 399 232 211 1060 56 352 742 85
Cuts at: 4528 5599 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 1060 742 399 352 232 211
85 56
BspEI T'CCGG_A
Cuts at: 2207 2233 6045 2207
Size: 26 3812 2544
Fragments arranged by size:
3812 2544 26
BspHI T'CATG_A
Cuts at: 2632 4271 6001 2632
Size: 1639 1730 3013
Fragments arranged by size:
3013 1730 1639
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2693 3737 844
Size: 1849 1044 3489
Fragments arranged by size:
3489 1849 1044
BsrBI CCG'CTC
Cuts at: 1394 1823 4269 1394
Size: 429 2446 3507
Fragments arranged by size:
3507 2446 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4877 5051 65
Size: 1183 3629 174 1396
Fragments arranged by size:
3629 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2289 2670 5026 5213 1700
Size: 110 479 381 2356 187 2869
Fragments arranged by size:
2869 2356 479 381 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6382
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6382
BssSI C'ACGA_G
Cuts at: 2078 3150 4442 5454 2078
Size: 1072 1292 1012 3006
Fragments arranged by size:
3006 1292 1072 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6382
BstBI TT'CG_AA
Cuts at: 5186 5186
Size: 6382
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6382
BstXI CCAn_nnnn'nTGG
Cuts at: 2030 3270 2030
Size: 1240 5142
BstYI r'GATC_y
Cuts at: 1972 2716 3712 4079 4464 4481 5933 6019 6031
Size: 744 996 367 385 17 1452 86 12
Cuts at: 6031 6377 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 996 744 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2859 2859
Size: 6382
Bsu36I CC'TnA_GG
Cuts at: 3637 3637
Size: 6382
BtgI C'CryG_G
Cuts at: 513 1065 2404 2475 2506 3229 513
Size: 552 1339 71 31 723 3666
Fragments arranged by size:
3666 1339 723 552 71 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2169 487
Size: 683 56 943 4700
Fragments arranged by size:
4700 943 683 56
BtsI GCAGTG_nn'
Cuts at: 3523 3898 4692 4712 3523
Size: 375 794 20 5193
Fragments arranged by size:
5193 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6382
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6347
DraI TTT'AAA
Cuts at: 3686 3686
Size: 6382
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5389 809
Size: 507 4073 1802
Fragments arranged by size:
4073 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3829 4718
Size: 54 1425 50 275 18 216 1783 889
Cuts at: 4718 6347 8
Size: 1629 43
Fragments arranged by size:
1783 1629 1425 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3829 1537
Size: 275 234 1783 4090
Fragments arranged by size:
4090 1783 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 3285 4312 1353
Size: 1932 1027 3423
Fragments arranged by size:
3423 1932 1027
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2242 4969 5343 5489 387
Size: 1064 138 653 2727 374 146 1280
Fragments arranged by size:
2727 1280 1064 653 374 146 138
EcoICRI GAG'CTC
Cuts at: 2231 3347 2231
Size: 1116 5266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2052 2247 2595 2646 3228 3333 3427
Size: 393 420 195 348 51 582 105 94
Cuts at: 3427 4445 5042 5829 6097 1239
Size: 1018 597 787 268 1524
Fragments arranged by size:
1524 1018 787 597 582 420 393 348
268 195 105 94 51
EcoO109I rG'GnC_Cy
Cuts at: 2019 2019
Size: 6382
EcoRI G'AATT_C
Cuts at: 3332 3691 4069 3332
Size: 359 378 5645
Fragments arranged by size:
5645 378 359
EcoRV GAT'ATC
Cuts at: 2966 2966
Size: 6382
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2600 2905 3063 2600
Size: 305 158 5919
Fragments arranged by size:
5919 305 158
FspI TGC'GCA
Cuts at: 4888 4888
Size: 6382
HaeII r_GCGC'y
Cuts at: 1678 2181 2367 2514 5529 1678
Size: 503 186 147 3015 2531
Fragments arranged by size:
3015 2531 503 186 147
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2322 2354 2582 2614 3556
Size: 32 135 32 750 32 228 32 942
Cuts at: 3556 3588 5023 5055 5097 5129 1373
Size: 32 1435 32 42 32 2626
Fragments arranged by size:
2626 1435 942 750 228 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2922 3529 3726 3972 669
Size: 988 281 984 607 197 246 3079
Fragments arranged by size:
3079 988 984 607 281 246 197
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6382
HpaI GTT'AAC
Cuts at: 2922 3972 2922
Size: 1050 5332
KasI G'GCGC_C
Cuts at: 2177 2510 2177
Size: 333 6049
KpnI G_GTAC'C
Cuts at: 3707 3707
Size: 6382
MfeI C'AATT_G
Cuts at: 3981 3981
Size: 6382
MluI A'CGCG_T
Cuts at: 6258 6258
Size: 6382
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3641 5496 5680 1305
Size: 2336 1855 184 2007
Fragments arranged by size:
2336 2007 1855 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4560
Fragments arranged by size:
4560 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2912 3117 3480 3588 4340 4699 4858
Size: 648 1746 205 363 108 752 359 159
Cuts at: 4858 518
Size: 2042
Fragments arranged by size:
2042 1746 752 648 363 359 205 159
108
MspA1I CmG'CkG
Cuts at: 1606 2406 2477 3256 3798 4475 5623 5868 1606
Size: 800 71 779 542 677 1148 245 2120
Fragments arranged by size:
2120 1148 800 779 677 542 245 71
NaeI GCC'GGC
Cuts at: 2291 2291
Size: 6382
NarI GG'CG_CC
Cuts at: 2178 2511 2178
Size: 333 6049
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5830
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6382
NgoMIV G'CCGG_C
Cuts at: 2289 2289
Size: 6382
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6382
NotI GC'GGCC_GC
Cuts at: 3829 3829
Size: 6382
NspI r_CATG'y
Cuts at: 1132 1229 3148 3740 5285 1132
Size: 97 1919 592 1545 2229
Fragments arranged by size:
2229 1919 1545 592 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6382
PciI A'CATG_T
Cuts at: 3144 5281 3144
Size: 2137 4245
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6382
PmeI GTTT'AAAC
Cuts at: 3686 3686
Size: 6382
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4451 4483 5964 5996 4451
Size: 32 1481 32 4837
Fragments arranged by size:
4837 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6382
PsiI TTA'TAA
Cuts at: 3952 3952
Size: 6382
PspOMI G'GGCC_C
Cuts at: 2018 2018
Size: 6382
PspXI vC'TCGA_Gb
Cuts at: 2525 2525
Size: 6382
PstI C_TGCA'G
Cuts at: 830 2312 3734 830
Size: 1482 1422 3478
Fragments arranged by size:
3478 1482 1422
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6382
PvuII CAG'CTG
Cuts at: 3256 3256
Size: 6382
SacI G_AGCT'C
Cuts at: 2233 3349 2233
Size: 1116 5266
SacII CC_GC'GG
Cuts at: 2407 2478 2407
Size: 71 6311
SalI G'TCGA_C
Cuts at: 1936 3724 1936
Size: 1788 4594
SbfI CC_TGCA'GG
Cuts at: 3734 3734
Size: 6382
ScaI AGT'ACT
Cuts at: 2531 4630 6342 2531
Size: 2099 1712 2571
Fragments arranged by size:
2571 2099 1712
SfcI C'TryA_G
Cuts at: 826 1045 2308 3730 4865 5546 5737 826
Size: 219 1263 1422 1135 681 191 1471
Fragments arranged by size:
1471 1422 1263 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2334 2355 2334
Size: 21 6361
SfoI GGC'GCC
Cuts at: 2179 2512 2179
Size: 333 6049
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6382
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6382
SmaI CCC'GGG
Cuts at: 1872 2125 2259 2299 3709 1872
Size: 253 134 40 1410 4545
Fragments arranged by size:
4545 1410 253 134 40
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2525 3292 4486 5387 5649 820
Size: 197 926 582 767 1194 901 262 1553
Fragments arranged by size:
1553 1194 926 901 767 582 262 197
SnaBI TAC'GTA
Cuts at: 493 4173 493
Size: 3680 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6382
SphI G_CATG'C
Cuts at: 1132 3740 1132
Size: 2608 3774
SrfI GCCC'GGGC
Cuts at: 2299 2299
Size: 6382
SspI AAT'ATT
Cuts at: 5 52 4306 5
Size: 47 4254 2081
Fragments arranged by size:
4254 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6382
StyI C'CwwG_G
Cuts at: 513 1065 3821 513
Size: 552 2756 3074
Fragments arranged by size:
3074 2756 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2519 2758 2859 3276 4409 4426 4579
Size: 1255 939 239 101 417 1133 17 153
Cuts at: 4579 4764 5209 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1133 939 445 417 239 185
153 101 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2529 3317 3576
Size: 274 80 33 51 1443 552 788 259
Cuts at: 3576 4628 6340 96
Size: 1052 1712 138
Fragments arranged by size:
1712 1443 1052 788 552 274 259 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3336 3368 6342 6374 3336
Size: 32 2974 32 3344
Fragments arranged by size:
3344 2974 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6382
XbaI T'CTAG_A
Cuts at: 3718 3718
Size: 6382
XhoI C'TCGA_G
Cuts at: 1943 2525 1943
Size: 582 5800
XmaI C'CCGG_G
Cuts at: 1870 2123 2257 2297 3707 1870
Size: 253 134 40 1410 4545
Fragments arranged by size:
4545 1410 253 134 40
XmnI GAAnn'nnTTC
Cuts at: 3046 3463 4511 3046
Size: 417 1048 4917
Fragments arranged by size:
4917 1048 417
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4190 276
Size: 53 83 186 936 2656 2468
Fragments arranged by size:
2656 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsiWI BsmBI DraIII EcoNI FseI FspAI NruI NsiI
PacI PfoI PmlI PpuMI PsrI RsrII SanDI SapI
SexAI SwaI XcmI