Restriction map for
pFN21AE3228
(Circular) (Six-base) MAPSORT of: pFN21AE3228.seq Check: 4000 from: 1 to: 6265
pFN21AE3228 6265 bp
With 182 enzymes: SgfI *
June 19, 2019 18:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3608 1937
Size: 1671 4594
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 4392 4765 1563
Size: 2829 373 3063
Fragments arranged by size:
3063 2829 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2897 2927 4328 5712 5980 1632
Size: 1265 30 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 1265 268 30
AfeI AGC'GCT
Cuts at: 3110 3544 3110
Size: 434 5831
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 3562 5164 6141 1203
Size: 2359 1602 977 1327
Fragments arranged by size:
2359 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 2204 1166
Size: 1038 5227
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6265
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3299 3331 3299
Size: 32 6233
AlwNI CAG_nnn'CTG
Cuts at: 2082 2875 3100 4008 5580 2082
Size: 793 225 908 1572 2767
Fragments arranged by size:
2767 1572 908 793 225
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 3574 3800 3952 6061 1493
Size: 2081 226 152 2109 1697
Fragments arranged by size:
2109 2081 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 3590 1870
Size: 73 196 1451 4545
Fragments arranged by size:
4545 1451 196 73
BamHI G'GATC_C
Cuts at: 3595 3962 3595
Size: 367 5898
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2047 3496 3586 5041 618
Size: 325 217 627 260 1449 90 1455 1842
Fragments arranged by size:
1842 1455 1449 627 325 260 217 90
BanII G_rGCy'C
Cuts at: 1391 3584 1391
Size: 2193 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5294
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4456 4490 1903
Size: 34 50 34 2435 34 3678
Fragments arranged by size:
3678 2435 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 2640 3123 1548
Size: 1092 483 4690
Fragments arranged by size:
4690 1092 483
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2766 4876 136
Size: 107 122 71 631 605 1094 2110 1525
Fragments arranged by size:
2110 1525 1094 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2040 6260 2040
Size: 4220 2045
BlpI GC'TnA_GC
Cuts at: 3682 3682
Size: 6265
Bme1580I G_kGCm'C
Cuts at: 1790 2052 3501 4326 5482 1790
Size: 262 1449 825 1156 2573
Fragments arranged by size:
2573 1449 1156 825 262
BmgBI CAC'GTC
Cuts at: 1318 2656 1318
Size: 1338 4927
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3996 4954 442
Size: 416 53 83 399 24 2579 958 1753
Fragments arranged by size:
2579 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3401 1056
Size: 2345 3920
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2500 2771 3155 3538 4925 1239
Size: 1261 271 384 383 1387 2579
Fragments arranged by size:
2579 1387 1261 384 383 271
Bpu10I CC'TnA_GC
Cuts at: 2314 2314
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2344 4390 5255 5553 2344
Size: 2046 865 298 3056
Fragments arranged by size:
3056 2046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2734 4056 493
Size: 711 1530 1322 2702
Fragments arranged by size:
2702 1530 1322 711
BsaBI GATnn'nnATC
Cuts at: 2726 6259 2726
Size: 3533 2732
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2193 2985 3266 4072
Size: 53 83 186 936 660 792 281 806
Cuts at: 4072 4454 5089 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 806 792 660 635 382 281
186 83 53
BsaWI w'CCGG_w
Cuts at: 2298 4698 5096 5370 5517 5928 2298
Size: 2400 398 274 147 411 2635
Fragments arranged by size:
2635 2400 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6040
Fragments arranged by size:
6040 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2416 2879 3008 3023 3047 3233 3458 3541
Size: 1230 463 129 15 24 186 225 83
Cuts at: 3541 1186
Size: 3910
Fragments arranged by size:
3910 1230 463 225 186 129 83 24
15
BseYI C'CCAG_C
Cuts at: 3476 5468 5964 3476
Size: 1992 496 3777
Fragments arranged by size:
3777 1992 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2705 2756 3234 3307 1163
Size: 573 318 651 51 478 73 4121
Fragments arranged by size:
4121 651 573 478 318 73 51
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2928 3468 3715 4476 5504 1540
Size: 275 186 927 540 247 761 1028 2301
Fragments arranged by size:
2301 1028 927 761 540 275 247 186
BsiHKAI G_wGCw'C
Cuts at: 2781 2848 2915 4326 4411 5482 2781
Size: 67 67 1411 85 1071 3564
Fragments arranged by size:
3564 1411 1071 85 67 67
BsmI GAATG_Cn'
Cuts at: 3776 3869 3776
Size: 93 6172
BsmBI CGTCTCn'nnnn_
Cuts at: 3370 3370
Size: 6265
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2781 2848 2915 3501 3584 4326
Size: 399 262 729 67 67 586 83 742
Cuts at: 4326 4411 5482 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 729 586 399 262 85
83 67 67
BspEI T'CCGG_A
Cuts at: 2298 5928 2298
Size: 3630 2635
BspHI T'CATG_A
Cuts at: 2565 3372 4154 5884 2565
Size: 807 782 1730 2946
Fragments arranged by size:
2946 1730 807 782
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2892 3620 844
Size: 2048 728 3489
Fragments arranged by size:
3489 2048 728
BsrBI CCG'CTC
Cuts at: 1394 1823 3139 3370 4152 1394
Size: 429 1316 231 782 3507
Fragments arranged by size:
3507 1316 782 429 231
BsrDI GCAATG_nn'
Cuts at: 65 1248 2312 4760 4934 65
Size: 1183 1064 2448 174 1396
Fragments arranged by size:
2448 1396 1183 1064 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 4909 5096 1700
Size: 110 950 2149 187 2869
Fragments arranged by size:
2869 2149 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6265
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6265
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6265
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2500 2011
Size: 489 5776
BstYI r'GATC_y
Cuts at: 1972 2040 2078 3595 3962 4347 4364 5816 5902
Size: 68 38 1517 367 385 17 1452 86
Cuts at: 5902 5914 6260 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1517 1452 385 367 346 86 68
38 17 12
Bsu36I CC'TnA_GG
Cuts at: 3030 3030
Size: 6265
BtgI C'CryG_G
Cuts at: 513 1065 2052 2553 513
Size: 552 987 501 4225
Fragments arranged by size:
4225 987 552 501
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5526
Fragments arranged by size:
5526 683 56
BtsI GCAGTG_nn'
Cuts at: 2960 3781 4575 4595 2960
Size: 821 794 20 4630
Fragments arranged by size:
4630 821 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2984 5272 809
Size: 507 1668 2288 1802
Fragments arranged by size:
2288 1802 1668 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2925 3465 3712
Size: 54 1425 50 275 18 1095 540 247
Cuts at: 3712 4601 6230 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1095 889 540 275 247 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2925 3465 3712 1537
Size: 275 1113 540 247 4090
Fragments arranged by size:
4090 1113 540 275 247
EarI CTCTTCn'nnn_
Cuts at: 1353 2564 2903 3249 4195 1353
Size: 1211 339 346 946 3423
Fragments arranged by size:
3423 1211 946 346 339
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2258 4852 5226 5372 387
Size: 1064 138 669 2594 374 146 1280
Fragments arranged by size:
2594 1280 1064 669 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2500 2771 2897 2927 3155 3538 4328
Size: 393 868 271 126 30 228 383 790
Cuts at: 4328 4925 5712 5980 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 868 790 787 597 393 383 271
268 228 126 30
EcoO109I rG'GnC_Cy
Cuts at: 2064 2881 3097 3359 2064
Size: 817 216 262 4970
Fragments arranged by size:
4970 817 262 216
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2936 2966 3112 3546 5412 1678
Size: 1258 30 146 434 1866 2531
Fragments arranged by size:
2531 1866 1258 434 146 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 2525 2557 4906
Size: 32 135 32 825 32 96 32 2349
Cuts at: 4906 4938 4980 5012 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2349 825 135 96 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1992 5379 5563 1305
Size: 687 3387 184 2007
Fragments arranged by size:
3387 2007 687 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4443
Fragments arranged by size:
4443 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2120 2204 2732 4223 4582 4741 518
Size: 648 954 84 528 1491 359 159 2042
Fragments arranged by size:
2042 1491 954 648 528 359 159 84
MspA1I CmG'CkG
Cuts at: 1606 2905 3480 3508 3681 4358 5506 5751 1606
Size: 1299 575 28 173 677 1148 245 2120
Fragments arranged by size:
2120 1299 1148 677 575 245 173 28
NaeI GCC'GGC
Cuts at: 2762 2762
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2553 513
Size: 552 1488 4225
Fragments arranged by size:
4225 1488 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 2760 2760
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 3397 1052
Size: 2345 3920
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 3452 3623 5168 1132
Size: 97 2223 171 1545 2229
Fragments arranged by size:
2229 2223 1545 171 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 5164 5164
Size: 6265
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3100 3112 1108
Size: 1992 12 4261
Fragments arranged by size:
4261 1992 12
PfoI T'CCnGG_A
Cuts at: 3201 3533 3554 3201
Size: 332 21 5912
Fragments arranged by size:
5912 332 21
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PmlI CAC'GTG
Cuts at: 2734 2734
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4334 4366 5847 5879 4334
Size: 32 1481 32 4720
Fragments arranged by size:
4720 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2881 3097 3359 2881
Size: 216 262 5787
Fragments arranged by size:
5787 262 216
PshAI GACnn'nnGTC
Cuts at: 1613 3269 1613
Size: 1656 4609
PsiI TTA'TAA
Cuts at: 3835 3835
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3617 830
Size: 2787 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 2905 3480 3508 2905
Size: 575 28 5662
Fragments arranged by size:
5662 575 28
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SapI GCTCTTCn'nnn_
Cuts at: 2903 2903
Size: 6265
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 3062 4513 6225 3062
Size: 1451 1712 3102
Fragments arranged by size:
3102 1712 1451
SexAI A'CCwGG_T
Cuts at: 3099 3099
Size: 6265
SfcI C'TryA_G
Cuts at: 826 1045 2478 3613 4748 5429 5620 826
Size: 219 1433 1135 1135 681 191 1471
Fragments arranged by size:
1471 1433 1135 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 2141 3592 1872
Size: 269 1451 4545
Fragments arranged by size:
4545 1451 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2323 4369 5270 5532 820
Size: 197 926 380 2046 901 262 1553
Fragments arranged by size:
2046 1553 926 901 380 262 197
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6265
SphI G_CATG'C
Cuts at: 1132 3623 1132
Size: 2491 3774
SspI AAT'ATT
Cuts at: 5 52 4189 5
Size: 47 4137 2081
Fragments arranged by size:
4137 2081 47
StuI AGG'CCT
Cuts at: 1517 2814 1517
Size: 1297 4968
StyI C'CwwG_G
Cuts at: 513 1065 2553 3704 513
Size: 552 1488 1151 3074
Fragments arranged by size:
3074 1488 1151 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 4292 4309 4462 4647 5092 325
Size: 1255 455 2257 17 153 185 445 1498
Fragments arranged by size:
2257 1498 1255 455 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2247 2712 3060
Size: 274 80 33 51 1443 270 465 348
Cuts at: 3060 3151 4511 6223 96
Size: 91 1360 1712 138
Fragments arranged by size:
1712 1443 1360 465 348 274 270 138
91 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2293 2325 6225 6257 2293
Size: 32 3900 32 2301
Fragments arranged by size:
3900 2301 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3101 1316
Size: 1785 4480
XbaI T'CTAG_A
Cuts at: 3601 3601
Size: 6265
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 2139 3590 1870
Size: 269 1451 4545
Fragments arranged by size:
4545 1451 269
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI XcmI