Restriction map for
pFN21AE3227
(Circular) (Six-base) MAPSORT of: pFN21AE3227.seq Check: 8522 from: 1 to: 6265
pFN21AE3227 6265 bp
With 182 enzymes: SgfI *
March 28, 2012 14:21 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4075 278
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3444 3608 1937
Size: 1507 164 4594
Fragments arranged by size:
4594 1507 164
Acc65I G'GTAC_C
Cuts at: 3586 3586
Size: 6265
AclI AA'CG_TT
Cuts at: 1563 2643 4392 4765 1563
Size: 1080 1749 373 3063
Fragments arranged by size:
3063 1749 1080 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3168 3363 3481 4328 5712 5980 1632
Size: 1536 195 118 847 1384 268 1917
Fragments arranged by size:
1917 1536 1384 847 268 195 118
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6068
AflIII A'CryG_T
Cuts at: 1203 2028 2557 3073 5164 6141 1203
Size: 825 529 516 2091 977 1327
Fragments arranged by size:
2091 1327 977 825 529 516
AgeI A'CCGG_T
Cuts at: 5096 5096
Size: 6265
AhdI GACnn_n'nnGTC
Cuts at: 4994 4994
Size: 6265
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2467 1184
Size: 1283 4982
AlwNI CAG_nnn'CTG
Cuts at: 3215 3561 4008 5580 3215
Size: 346 447 1572 3900
Fragments arranged by size:
3900 1572 447 346
ApaI G_GGCC'C
Cuts at: 2012 2012
Size: 6265
ApaLI G'TGCA_C
Cuts at: 4322 5478 4322
Size: 1156 5109
ApoI r'AATT_y
Cuts at: 1493 2141 2456 3574 3800 3952 6061 1493
Size: 648 315 1118 226 152 2109 1697
Fragments arranged by size:
2109 1697 1118 648 315 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4819 160
Size: 872 3787 1606
Fragments arranged by size:
3787 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
AvaI C'yCGr_G
Cuts at: 1870 1943 3590 1870
Size: 73 1647 4545
Fragments arranged by size:
4545 1647 73
BamHI G'GATC_C
Cuts at: 2997 3595 3962 2997
Size: 598 367 5300
Fragments arranged by size:
5300 598 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3586 5041 618
Size: 325 217 627 1799 1455 1842
Fragments arranged by size:
1842 1799 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 3584 1391
Size: 621 1572 4072
Fragments arranged by size:
4072 1572 621
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3487 928
Size: 971 1588 3706
Fragments arranged by size:
3706 1588 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4456 4490 1903
Size: 34 2519 34 3678
Fragments arranged by size:
3678 2519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4157 5367 4157
Size: 1210 5055
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6265
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2540 4876 136
Size: 107 122 71 631 605 868 2336 1525
Fragments arranged by size:
2336 1525 868 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3141 6260 3141
Size: 3119 3146
BlpI GC'TnA_GC
Cuts at: 2524 3682 2524
Size: 1158 5107
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2968 4326 5482 1790
Size: 222 956 1358 1156 2573
Fragments arranged by size:
2573 1358 1156 956 222
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6265
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2315 3540 3996
Size: 416 53 83 399 24 898 1225 456
Cuts at: 3996 4954 442
Size: 958 1753
Fragments arranged by size:
1753 1225 958 898 456 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6265
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2897 4925 1239
Size: 1658 2028 2579
Fragments arranged by size:
2579 2028 1658
Bpu10I CC'TnA_GC
Cuts at: 2692 2692
Size: 6265
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 2469 2488 4390 5255 5553 2447
Size: 22 19 1902 865 298 3159
Fragments arranged by size:
3159 1902 865 298 22 19
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4928 882
Size: 617 3429 2219
Fragments arranged by size:
3429 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4056 493
Size: 711 2852 2702
Fragments arranged by size:
2852 2702 711
BsaBI GATnn'nnATC
Cuts at: 6259 6259
Size: 6265
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4072 4454 5089 275
Size: 53 83 186 936 2539 382 635 1451
Fragments arranged by size:
2539 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4698 5096 5370 5517 5928 4698
Size: 398 274 147 411 5035
Fragments arranged by size:
5035 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6040
Fragments arranged by size:
6040 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2472 1186
Size: 1286 4979
BseYI C'CCAG_C
Cuts at: 2056 2472 5468 5964 2056
Size: 416 2996 496 2357
Fragments arranged by size:
2996 2357 496 416
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2053 2108 1163
Size: 573 317 55 5320
Fragments arranged by size:
5320 573 317 55
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3715 4476 5504 1540
Size: 275 186 1714 761 1028 2301
Fragments arranged by size:
2301 1714 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4326 4411 5482 4326
Size: 85 1071 5109
Fragments arranged by size:
5109 1071 85
BsmI GAATG_Cn'
Cuts at: 2321 2547 2817 2887 3399 3776 3869 2321
Size: 226 270 70 512 377 93 4717
Fragments arranged by size:
4717 512 377 270 226 93 70
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2968 3584 4326 4411 5482 1391
Size: 399 222 956 616 742 85 1071 2174
Fragments arranged by size:
2174 1071 956 742 616 399 222 85
BspEI T'CCGG_A
Cuts at: 5928 5928
Size: 6265
BspHI T'CATG_A
Cuts at: 4154 5884 4154
Size: 1730 4535
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3620 844
Size: 2776 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4152 1394
Size: 429 2329 3507
Fragments arranged by size:
3507 2329 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 4760 4934 65
Size: 1183 1245 2267 174 1396
Fragments arranged by size:
2267 1396 1245 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2037 4909 5096 1700
Size: 110 227 2872 187 2869
Fragments arranged by size:
2872 2869 227 187 110
BsrGI T'GTAC_A
Cuts at: 96 2685 96
Size: 2589 3676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6265
BssSI C'ACGA_G
Cuts at: 4325 5337 4325
Size: 1012 5253
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2378 794
Size: 1584 4681
BstBI TT'CG_AA
Cuts at: 5069 5069
Size: 6265
BstEII G'GTnAC_C
Cuts at: 1184 2172 1184
Size: 988 5277
BstYI r'GATC_y
Cuts at: 1972 2997 3036 3141 3595 3962 4347 4364 5816
Size: 1025 39 105 454 367 385 17 1452
Cuts at: 5816 5902 5914 6260 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1025 454 385 367 346 105
86 39 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4774
Fragments arranged by size:
4774 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2710 487
Size: 683 56 1484 4042
Fragments arranged by size:
4042 1484 683 56
BtsI GCAGTG_nn'
Cuts at: 2239 2535 2583 2852 3781 4575 4595 2239
Size: 296 48 269 929 794 20 3909
Fragments arranged by size:
3909 929 794 296 269 48 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6265
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6230
DraI TTT'AAA
Cuts at: 3569 3569
Size: 6265
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5272 809
Size: 507 3956 1802
Fragments arranged by size:
3956 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3165 3712 4601
Size: 54 1425 50 275 18 1335 547 889
Cuts at: 4601 6230 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1335 889 547 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3712 1537
Size: 275 1900 4090
Fragments arranged by size:
4090 1900 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4195 1353
Size: 2842 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4852 5226 5372 387
Size: 1064 138 3263 374 146 1280
Fragments arranged by size:
3263 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2897 3168 3363 3481 4328 4925 5712
Size: 393 1265 271 195 118 847 597 787
Cuts at: 5712 5980 1239
Size: 268 1524
Fragments arranged by size:
1524 1265 847 787 597 393 271 268
195 118
EcoRI G'AATT_C
Cuts at: 3574 3952 3574
Size: 378 5887
FspI TGC'GCA
Cuts at: 4771 4771
Size: 6265
HaeII r_GCGC'y
Cuts at: 1678 2057 2120 2224 5412 1678
Size: 379 63 104 3188 2531
Fragments arranged by size:
3188 2531 379 104 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 2855 2887 4906
Size: 32 135 32 770 32 481 32 2019
Cuts at: 4906 4938 4980 5012 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2019 770 481 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3609 3855 669
Size: 988 281 1671 246 3079
Fragments arranged by size:
3079 1671 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6265
HpaI GTT'AAC
Cuts at: 3855 3855
Size: 6265
KpnI G_GTAC'C
Cuts at: 3590 3590
Size: 6265
MfeI C'AATT_G
Cuts at: 3864 3864
Size: 6265
MluI A'CGCG_T
Cuts at: 6141 6141
Size: 6265
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5379 5563 1305
Size: 4074 184 2007
Fragments arranged by size:
4074 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3167 10
Size: 54 1425 343 1335 3108
Fragments arranged by size:
3108 1425 1335 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2773 2843 2961 3051 3186 3372 4223
Size: 648 1607 70 118 90 135 186 851
Cuts at: 4223 4582 4741 518
Size: 359 159 2042
Fragments arranged by size:
2042 1607 851 648 359 186 159 135
118 90 70
MspA1I CmG'CkG
Cuts at: 1606 3501 3681 4358 5506 5751 1606
Size: 1895 180 677 1148 245 2120
Fragments arranged by size:
2120 1895 1148 677 245 180
NaeI GCC'GGC
Cuts at: 2039 2039
Size: 6265
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4774
Fragments arranged by size:
4774 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6265
NgoMIV G'CCGG_C
Cuts at: 2037 2037
Size: 6265
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6265
NotI GC'GGCC_GC
Cuts at: 3712 3712
Size: 6265
NspI r_CATG'y
Cuts at: 1132 1229 2561 2772 2848 3077 3623 5168 1132
Size: 97 1332 211 76 229 546 1545 2229
Fragments arranged by size:
2229 1545 1332 546 229 211 97 76
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6265
PciI A'CATG_T
Cuts at: 2557 3073 5164 2557
Size: 516 2091 3658
Fragments arranged by size:
3658 2091 516
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3007 1108
Size: 1899 4366
PmeI GTTT'AAAC
Cuts at: 3569 3569
Size: 6265
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2507 2539 4334 4366 5847 5879 2507
Size: 32 1795 32 1481 32 2893
Fragments arranged by size:
2893 1795 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6265
PsiI TTA'TAA
Cuts at: 2355 3835 2355
Size: 1480 4785
PspOMI G'GGCC_C
Cuts at: 2008 2008
Size: 6265
PstI C_TGCA'G
Cuts at: 830 3617 830
Size: 2787 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6265
PvuII CAG'CTG
Cuts at: 3501 3501
Size: 6265
SalI G'TCGA_C
Cuts at: 1936 3607 1936
Size: 1671 4594
SbfI CC_TGCA'GG
Cuts at: 3617 3617
Size: 6265
ScaI AGT'ACT
Cuts at: 2137 4513 6225 2137
Size: 2376 1712 2177
Fragments arranged by size:
2376 2177 1712
SfcI C'TryA_G
Cuts at: 826 1045 2178 2710 2796 3613 4748 5429 5620
Size: 219 1133 532 86 817 1135 681 191
Cuts at: 5620 826
Size: 1471
Fragments arranged by size:
1471 1135 1133 817 681 532 219 191
86
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6265
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6265
SmaI CCC'GGG
Cuts at: 1872 3592 1872
Size: 1720 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2426 2467 2484 4369 5270 5532
Size: 197 926 483 41 17 1885 901 262
Cuts at: 5532 820
Size: 1553
Fragments arranged by size:
1885 1553 926 901 483 262 197 41
17
SnaBI TAC'GTA
Cuts at: 493 4056 493
Size: 3563 2702
SpeI A'CTAG_T
Cuts at: 152 3406 152
Size: 3254 3011
SphI G_CATG'C
Cuts at: 1132 2772 3623 1132
Size: 1640 851 3774
Fragments arranged by size:
3774 1640 851
SspI AAT'ATT
Cuts at: 5 52 2830 4189 5
Size: 47 2778 1359 2081
Fragments arranged by size:
2778 2081 1359 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6265
StyI C'CwwG_G
Cuts at: 513 1065 2004 2906 3704 513
Size: 552 939 902 798 3074
Fragments arranged by size:
3074 939 902 798 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4292 4309 4462 4647 5092 325
Size: 1255 2712 17 153 185 445 1498
Fragments arranged by size:
2712 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2135 2685 3196
Size: 274 80 33 51 1443 158 550 511
Cuts at: 3196 4511 6223 96
Size: 1315 1712 138
Fragments arranged by size:
1712 1443 1315 550 511 274 158 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6225 6257 6225
Size: 32 6233
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6265
XbaI T'CTAG_A
Cuts at: 2614 3022 3601 2614
Size: 408 579 5278
Fragments arranged by size:
5278 579 408
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6265
XmaI C'CCGG_G
Cuts at: 1870 3590 1870
Size: 1720 4545
XmnI GAAnn'nnTTC
Cuts at: 4394 4394
Size: 6265
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4073 276
Size: 53 83 186 936 2539 2468
Fragments arranged by size:
2539 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI