Restriction map for
pFN21AE3224
(Circular) (Six-base) MAPSORT of: pFN21AE3224.seq Check: 9785 from: 1 to: 6235
pFN21AE3224 6235 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3182 3182
Size: 6235
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3578 1937
Size: 1641 4594
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2159 3088 3329 4298 5682 5950 1632
Size: 527 929 241 969 1384 268 1917
Fragments arranged by size:
1917 1384 969 929 527 268 241
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6038
AflIII A'CryG_T
Cuts at: 1203 5134 6111 1203
Size: 3931 977 1327
Fragments arranged by size:
3931 1327 977
AgeI A'CCGG_T
Cuts at: 2584 5066 2584
Size: 2482 3753
AhdI GACnn_n'nnGTC
Cuts at: 4964 4964
Size: 6235
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2580 1184
Size: 1396 4839
AlwNI CAG_nnn'CTG
Cuts at: 2323 2431 2573 3978 5550 2323
Size: 108 142 1405 1572 3008
Fragments arranged by size:
3008 1572 1405 142 108
ApaI G_GGCC'C
Cuts at: 2504 3440 3452 3461 2504
Size: 936 12 9 5278
Fragments arranged by size:
5278 936 12 9
ApaLI G'TGCA_C
Cuts at: 2608 4292 5448 2608
Size: 1684 1156 3395
Fragments arranged by size:
3395 1684 1156
ApoI r'AATT_y
Cuts at: 1493 3544 3770 3922 6031 1493
Size: 2051 226 152 2109 1697
Fragments arranged by size:
2109 2051 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2660 2660
Size: 6235
AseI AT'TA_AT
Cuts at: 160 1032 4789 160
Size: 872 3757 1606
Fragments arranged by size:
3757 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2528 2603 2666 3001 3560 1870
Size: 73 109 476 75 63 335 559 4545
Fragments arranged by size:
4545 559 476 335 109 75 73 63
BamHI G'GATC_C
Cuts at: 3390 3565 3932 3390
Size: 175 367 5693
Fragments arranged by size:
5693 367 175
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2119 2872 2936 3556 5011
Size: 325 217 627 332 753 64 620 1455
Cuts at: 5011 618
Size: 1842
Fragments arranged by size:
1842 1455 753 627 620 332 325 217
64
BanII G_rGCy'C
Cuts at: 1391 2504 2580 2841 3271 3440 3452 3461 3554
Size: 1113 76 261 430 169 12 9 93
Cuts at: 3554 1391
Size: 4072
Fragments arranged by size:
4072 1113 430 261 169 93 76 12
9
BbeI G_GCGC'C
Cuts at: 2876 2876
Size: 6235
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2021 928
Size: 971 122 5142
Fragments arranged by size:
5142 971 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3266 3286 3300 3320 4426 4460 1903
Size: 34 1329 20 14 20 1106 34 3678
Fragments arranged by size:
3678 1329 1106 34 34 20 20 14
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6235
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2632 2727 2934
Size: 107 122 71 631 605 960 95 207
Cuts at: 2934 3266 3456 4846 136
Size: 332 190 1390 1525
Fragments arranged by size:
1525 1390 960 631 605 332 207 190
122 107 95 71
BglII A'GATC_T
Cuts at: 2464 3417 6230 2464
Size: 953 2813 2469
Fragments arranged by size:
2813 2469 953
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 2124 2504 2603 2612 2852 2939 3440 3452
Size: 334 380 99 9 240 87 501 12
Cuts at: 3452 3461 4296 5452 1790
Size: 9 835 1156 2573
Fragments arranged by size:
2573 1156 835 501 380 334 240 99
87 12 9 9
BmgBI CAC'GTC
Cuts at: 1318 2800 3193 1318
Size: 1482 393 4360
Fragments arranged by size:
4360 1482 393
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3343 3966 4924
Size: 416 53 83 399 24 1926 623 958
Cuts at: 4924 442
Size: 1753
Fragments arranged by size:
1926 1753 958 623 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2528 3035 4895 1239
Size: 1289 507 1860 2579
Fragments arranged by size:
2579 1860 1289 507
Bpu10I CC'TnA_GC
Cuts at: 2042 2574 2042
Size: 532 5703
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2240 4360 5225 5523 2240
Size: 2120 865 298 2952
Fragments arranged by size:
2952 2120 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2155 2325 2333 2971 4898 882
Size: 617 656 170 8 638 1927 2219
Fragments arranged by size:
2219 1927 656 638 617 170 8
BsaAI yAC'GTr
Cuts at: 493 1204 4026 493
Size: 711 2822 2702
Fragments arranged by size:
2822 2702 711
BsaBI GATnn'nnATC
Cuts at: 6229 6229
Size: 6235
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2249 2873 4042 4424
Size: 53 83 186 936 716 624 1169 382
Cuts at: 4424 5059 275
Size: 635 1451
Fragments arranged by size:
1451 1169 936 716 635 624 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2584 4668 5066 5340 5487 5898 2584
Size: 2084 398 274 147 411 2921
Fragments arranged by size:
2921 2084 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6010
Fragments arranged by size:
6010 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2720 2723 2747 2750 2830 1186
Size: 1534 3 24 3 80 4591
Fragments arranged by size:
4591 1534 80 24 3 3
BseYI C'CCAG_C
Cuts at: 3423 3444 5438 5934 3423
Size: 21 1994 496 3724
Fragments arranged by size:
3724 1994 496 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2330 2891 3382 1163
Size: 573 594 561 491 4016
Fragments arranged by size:
4016 594 573 561 491
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3685 4446 5474 1540
Size: 275 186 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2612 3002 4296 4381 5452 2612
Size: 390 1294 85 1071 3395
Fragments arranged by size:
3395 1294 1071 390 85
BsmI GAATG_Cn'
Cuts at: 3746 3839 3746
Size: 93 6142
BsmBI CGTCTCn'nnnn_
Cuts at: 2256 2256
Size: 6235
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2504 2580 2603 2612 2841 2852
Size: 399 334 380 76 23 9 229 11
Cuts at: 2852 2939 3002 3271 3440 3452 3461 3554 4296
Size: 87 63 269 169 12 9 93 742
Cuts at: 4296 4381 5452 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 380 334 269 229
169 93 87 85 76 63 23 12
11 9 9
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2799 3182 3590 844
Size: 1955 383 408 3489
Fragments arranged by size:
3489 1955 408 383
BsrBI CCG'CTC
Cuts at: 1394 1823 2443 4122 1394
Size: 429 620 1679 3507
Fragments arranged by size:
3507 1679 620 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2091 3081 3112 4730 4904 65
Size: 1183 843 990 31 1618 174 1396
Fragments arranged by size:
1618 1396 1183 990 843 174 31
BsrFI r'CCGG_y
Cuts at: 1700 1810 2584 4879 5066 1700
Size: 110 774 2295 187 2869
Fragments arranged by size:
2869 2295 774 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 2660 2717 1925
Size: 735 57 5443
Fragments arranged by size:
5443 735 57
BssSI C'ACGA_G
Cuts at: 2328 2995 4295 5307 2328
Size: 667 1300 1012 3256
Fragments arranged by size:
3256 1300 1012 667
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 2925 3405 794
Size: 1325 806 480 3624
Fragments arranged by size:
3624 1325 806 480
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 3427 1184
Size: 2243 3992
BstXI CCAn_nnnn'nTGG
Cuts at: 2104 2104
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 2464 3390 3417 3565 3932 4317 4334 5786
Size: 492 926 27 148 367 385 17 1452
Cuts at: 5786 5872 5884 6230 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 926 492 385 367 346 148
86 27 17 12
Bsu36I CC'TnA_GG
Cuts at: 2145 2424 3045 2145
Size: 279 621 5335
Fragments arranged by size:
5335 621 279
BtgI C'CryG_G
Cuts at: 513 1065 2004 2353 2495 2886 513
Size: 552 939 349 142 391 3862
Fragments arranged by size:
3862 939 552 391 349 142
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 2064 2309 2574 2603 3103 3751 4545 4565 2064
Size: 245 265 29 500 648 794 20 3734
Fragments arranged by size:
3734 794 648 500 265 245 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2277 2312 529
Size: 35 1713 35 4452
Fragments arranged by size:
4452 1713 35 35
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2248 5242 809
Size: 507 932 2994 1802
Fragments arranged by size:
2994 1802 932 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200
Size: 54 1425 50 275 18 1852 889 1629
Cuts at: 6200 8
Size: 43
Fragments arranged by size:
1852 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4165 1353
Size: 2812 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2975 4822 5196 5342 387
Size: 1064 138 1386 1847 374 146 1280
Fragments arranged by size:
1847 1386 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2159 2528 3035 3088 3329 4298 4895
Size: 393 527 369 507 53 241 969 597
Cuts at: 4895 5682 5950 1239
Size: 787 268 1524
Fragments arranged by size:
1524 969 787 597 527 507 393 369
268 241 53
EcoNI CCTnn'n_nnAGG
Cuts at: 2917 2917
Size: 6235
EcoO109I rG'GnC_Cy
Cuts at: 2479 2500 2501 2516 2533 2680 3253 3436 3448
Size: 21 1 15 17 147 573 183 12
Cuts at: 3448 3449 3457 2479
Size: 1 8 5257
Fragments arranged by size:
5257 573 183 147 21 17 15 12
8 1 1
EcoRI G'AATT_C
Cuts at: 3544 3922 3544
Size: 378 5857
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 2744 2876 3035 5382 1678
Size: 1066 132 159 2347 2531
Fragments arranged by size:
2531 2347 1066 159 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4876 4908 4950 4982 1373
Size: 32 135 32 3304 32 42 32 2626
Fragments arranged by size:
3304 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3196 3579 3825 669
Size: 988 281 1258 383 246 3079
Fragments arranged by size:
3079 1258 988 383 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KasI G'GCGC_C
Cuts at: 2872 2872
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 3834 3834
Size: 6235
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5349 5533 1305
Size: 4044 184 2007
Fragments arranged by size:
4044 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2352 4193 4552 4711 518
Size: 648 1186 1841 359 159 2042
Fragments arranged by size:
2042 1841 1186 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2320 2437 2449 2497 2761 2806 3396 3474
Size: 714 117 12 48 264 45 590 78
Cuts at: 3474 3486 3651 4328 5476 5721 1606
Size: 12 165 677 1148 245 2120
Fragments arranged by size:
2120 1148 714 677 590 264 245 165
117 78 48 45 12 12
NarI GG'CG_CC
Cuts at: 2873 2873
Size: 6235
NcoI C'CATG_G
Cuts at: 513 1065 2004 2353 513
Size: 552 939 349 4395
Fragments arranged by size:
4395 939 552 349
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3593 5138 1132
Size: 97 2364 1545 2229
Fragments arranged by size:
2364 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3452 1348
Size: 2104 4131
PciI A'CATG_T
Cuts at: 5134 5134
Size: 6235
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3361 1108
Size: 2253 3982
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2479 2533 2680 3253 2479
Size: 54 147 573 5461
Fragments arranged by size:
5461 573 147 54
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 3805 3805
Size: 6235
PspOMI G'GGCC_C
Cuts at: 2500 3436 3448 3457 2500
Size: 936 12 9 5278
Fragments arranged by size:
5278 936 12 9
PstI C_TGCA'G
Cuts at: 830 2436 2493 2796 2921 2934 3324 3491 3587
Size: 1606 57 303 125 13 390 167 96
Cuts at: 3587 830
Size: 3478
Fragments arranged by size:
3478 1606 390 303 167 125 96 57
13
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
PvuII CAG'CTG
Cuts at: 2449 2806 2449
Size: 357 5878
SacII CC_GC'GG
Cuts at: 2498 2498
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SanDI GG'GwC_CC
Cuts at: 2533 2533
Size: 6235
SbfI CC_TGCA'GG
Cuts at: 2934 3587 2934
Size: 653 5582
ScaI AGT'ACT
Cuts at: 4483 6195 4483
Size: 1712 4523
SexAI A'CCwGG_T
Cuts at: 2676 2676
Size: 6235
SfcI C'TryA_G
Cuts at: 826 1045 2432 2489 2792 2917 2930 3320 3487
Size: 219 1387 57 303 125 13 390 167
Cuts at: 3487 3583 4718 5399 5590 826
Size: 96 1135 681 191 1471
Fragments arranged by size:
1471 1387 1135 681 390 303 219 191
167 125 96 57 13
SfiI GGCCn_nnn'nGGCC
Cuts at: 3456 3456
Size: 6235
SfoI GGC'GCC
Cuts at: 2874 2874
Size: 6235
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 2584 1700
Size: 884 5351
SmaI CCC'GGG
Cuts at: 1872 2530 2605 3562 1872
Size: 658 75 957 4545
Fragments arranged by size:
4545 957 658 75
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2255 4339 5240 5502 820
Size: 197 926 312 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 312 262 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 4159 5
Size: 47 4107 2081
Fragments arranged by size:
4107 2081 47
StuI AGG'CCT
Cuts at: 1517 2672 1517
Size: 1155 5080
StyI C'CwwG_G
Cuts at: 513 1065 2004 2353 3432 3674 513
Size: 552 939 349 1079 242 3074
Fragments arranged by size:
3074 1079 939 552 349 242
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2167 2296 3528 4262 4279 4432 4617
Size: 1255 587 129 1232 734 17 153 185
Cuts at: 4617 5062 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1232 734 587 445 185 153
129 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4481 6193 96
Size: 274 80 33 51 1443 2504 1712 138
Fragments arranged by size:
2504 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6195 6227 6195
Size: 32 6203
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3064 3571 3064
Size: 507 5728
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2589 2589
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 2528 2603 3560 1870
Size: 658 75 957 4545
Fragments arranged by size:
4545 957 658 75
XmnI GAAnn'nnTTC
Cuts at: 4364 4364
Size: 6235
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AvrII BaeI BbvCI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SapI SrfI SwaI