Restriction map for
pFN21AE3222
(Circular) (Six-base) MAPSORT of: pFN21AE3222.seq Check: 3112 from: 1 to: 6217
pFN21AE3222 6217 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4027 278
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3560 1937
Size: 1623 4594
Acc65I G'GTAC_C
Cuts at: 3538 3538
Size: 6217
AclI AA'CG_TT
Cuts at: 1563 2550 4344 4717 1563
Size: 987 1794 373 3063
Fragments arranged by size:
3063 1794 987 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3307 4280 5664 5932 1632
Size: 1675 973 1384 268 1917
Fragments arranged by size:
1917 1675 1384 973 268
AflII C'TTAA_G
Cuts at: 820 1017 2015 820
Size: 197 998 5022
Fragments arranged by size:
5022 998 197
AflIII A'CryG_T
Cuts at: 1203 5116 6093 1203
Size: 3913 977 1327
Fragments arranged by size:
3913 1327 977
AgeI A'CCGG_T
Cuts at: 5048 5048
Size: 6217
AhdI GACnn_n'nnGTC
Cuts at: 4946 4946
Size: 6217
AleI CACnn'nnGTG
Cuts at: 1166 2165 1166
Size: 999 5218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6217
AlwNI CAG_nnn'CTG
Cuts at: 2899 3413 3960 5532 2899
Size: 514 547 1572 3584
Fragments arranged by size:
3584 1572 547 514
ApaLI G'TGCA_C
Cuts at: 4274 5430 4274
Size: 1156 5061
ApoI r'AATT_y
Cuts at: 1493 2312 2331 2795 3188 3526 3752 3904 6013
Size: 819 19 464 393 338 226 152 2109
Cuts at: 6013 1493
Size: 1697
Fragments arranged by size:
2109 1697 819 464 393 338 226 152
19
AseI AT'TA_AT
Cuts at: 160 1032 4771 160
Size: 872 3739 1606
Fragments arranged by size:
3739 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
AvaI C'yCGr_G
Cuts at: 1870 1943 3105 3513 3542 1870
Size: 73 1162 408 29 4545
Fragments arranged by size:
4545 1162 408 73 29
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2376 2409 2668 2701 2376
Size: 33 259 33 5892
Fragments arranged by size:
5892 259 33 33
BamHI G'GATC_C
Cuts at: 3547 3914 3547
Size: 367 5850
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3538 4993 618
Size: 325 217 627 1751 1455 1842
Fragments arranged by size:
1842 1751 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2652 2691 2769 3438 3536 1391
Size: 1261 39 78 669 98 4072
Fragments arranged by size:
4072 1261 669 98 78 39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2411 928
Size: 971 512 4734
Fragments arranged by size:
4734 971 512
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3086 3120 4408 4442 1903
Size: 34 1149 34 1288 34 3678
Fragments arranged by size:
3678 1288 1149 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2669 4109 5319 2669
Size: 1440 1210 3567
Fragments arranged by size:
3567 1440 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6217
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4828 136
Size: 107 122 71 631 605 3156 1525
Fragments arranged by size:
3156 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6212 6212
Size: 6217
BlpI GC'TnA_GC
Cuts at: 3093 3634 3093
Size: 541 5676
Bme1580I G_kGCm'C
Cuts at: 1790 2141 2718 4278 5434 1790
Size: 351 577 1560 1156 2573
Fragments arranged by size:
2573 1560 1156 577 351
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6217
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3948 4906 442
Size: 416 53 83 399 24 2531 958 1753
Fragments arranged by size:
2531 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6217
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 6217
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2299 2835 2940 3045 3390 4877 1239
Size: 1060 536 105 105 345 1487 2579
Fragments arranged by size:
2579 1487 1060 536 345 105 105
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 4342 5207 5505 2288
Size: 2054 865 298 3000
Fragments arranged by size:
3000 2054 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2072 4880 882
Size: 617 573 2808 2219
Fragments arranged by size:
2808 2219 617 573
BsaAI yAC'GTr
Cuts at: 493 1204 4008 493
Size: 711 2804 2702
Fragments arranged by size:
2804 2702 711
BsaBI GATnn'nnATC
Cuts at: 2502 2948 6211 2502
Size: 446 3263 2508
Fragments arranged by size:
3263 2508 446
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4024 4406 5041 275
Size: 53 83 186 936 2491 382 635 1451
Fragments arranged by size:
2491 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4650 5048 5322 5469 5880 4650
Size: 398 274 147 411 4987
Fragments arranged by size:
4987 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2039 2069 2364 2394 1178
Size: 30 165 30 636 30 295 30 5001
Fragments arranged by size:
5001 636 295 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2777 3494 1186
Size: 1591 717 3909
Fragments arranged by size:
3909 1591 717
BseYI C'CCAG_C
Cuts at: 3274 5420 5916 3274
Size: 2146 496 3575
Fragments arranged by size:
3575 2146 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2018 2582 3009 1163
Size: 573 282 564 427 4371
Fragments arranged by size:
4371 573 564 427 282
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3667 4428 5456 1540
Size: 275 186 1666 761 1028 2301
Fragments arranged by size:
2301 1666 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2691 2769 3504 4278 4363 5434 2691
Size: 78 735 774 85 1071 3474
Fragments arranged by size:
3474 1071 774 735 85 78
BsmI GAATG_Cn'
Cuts at: 3728 3821 3728
Size: 93 6124
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2141 2652 2691 2718 2769 3438 3504
Size: 399 351 511 39 27 51 669 66
Cuts at: 3504 3536 4278 4363 5434 1391
Size: 32 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 669 511 399 351 85
66 51 39 32 27
BspEI T'CCGG_A
Cuts at: 5880 5880
Size: 6217
BspHI T'CATG_A
Cuts at: 2665 4106 5836 2665
Size: 1441 1730 3046
Fragments arranged by size:
3046 1730 1441
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3572 844
Size: 2728 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4104 1394
Size: 429 2281 3507
Fragments arranged by size:
3507 2281 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4712 4886 65
Size: 1183 3464 174 1396
Fragments arranged by size:
3464 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4861 5048 1700
Size: 110 3051 187 2869
Fragments arranged by size:
3051 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6217
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6217
BssSI C'ACGA_G
Cuts at: 4277 5289 4277
Size: 1012 5205
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6217
BstBI TT'CG_AA
Cuts at: 3316 5021 3316
Size: 1705 4512
BstEII G'GTnAC_C
Cuts at: 1184 2456 1184
Size: 1272 4945
BstXI CCAn_nnnn'nTGG
Cuts at: 2118 2118
Size: 6217
BstYI r'GATC_y
Cuts at: 1972 2943 3547 3914 4299 4316 5768 5854 5866
Size: 971 604 367 385 17 1452 86 12
Cuts at: 5866 6212 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 971 604 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2058 3364 513
Size: 552 939 54 1306 3366
Fragments arranged by size:
3366 1306 939 552 54
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5478
Fragments arranged by size:
5478 683 56
BtsI GCAGTG_nn'
Cuts at: 2030 3144 3733 4527 4547 2030
Size: 1114 589 794 20 3700
Fragments arranged by size:
3700 1114 794 589 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6217
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2496 2531 529
Size: 35 1932 35 4215
Fragments arranged by size:
4215 1932 35 35
DraI TTT'AAA
Cuts at: 3521 3521
Size: 6217
DraIII CAC_nnn'GTG
Cuts at: 3491 3491
Size: 6217
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2199 5224 809
Size: 507 883 3025 1802
Fragments arranged by size:
3025 1802 883 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3664 4553 6182
Size: 54 1425 50 275 18 1834 889 1629
Cuts at: 6182 8
Size: 43
Fragments arranged by size:
1834 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3664 1537
Size: 275 1852 4090
Fragments arranged by size:
4090 1852 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2017 2075 4147 1353
Size: 664 58 2072 3423
Fragments arranged by size:
3423 2072 664 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4804 5178 5324 387
Size: 1064 138 3215 374 146 1280
Fragments arranged by size:
3215 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2689 2767 2689
Size: 78 6139
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2299 2835 2940 3045 3307 3390 4280
Size: 393 667 536 105 105 262 83 890
Cuts at: 4280 4877 5664 5932 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 890 787 667 597 536 393 268
262 105 105 83
EcoO109I rG'GnC_Cy
Cuts at: 2810 3109 2810
Size: 299 5918
EcoRI G'AATT_C
Cuts at: 3526 3904 3526
Size: 378 5839
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2491 2645 3442 2491
Size: 154 797 5266
Fragments arranged by size:
5266 797 154
FspI TGC'GCA
Cuts at: 4723 4723
Size: 6217
HaeII r_GCGC'y
Cuts at: 1678 5364 1678
Size: 3686 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2037 2069 2986 3018 3236
Size: 32 135 32 465 32 917 32 218
Cuts at: 3236 3268 4858 4890 4932 4964 1373
Size: 32 1590 32 42 32 2626
Fragments arranged by size:
2626 1590 917 465 218 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3561 3807 669
Size: 988 281 1623 246 3079
Fragments arranged by size:
3079 1623 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6217
HpaI GTT'AAC
Cuts at: 3807 3807
Size: 6217
KpnI G_GTAC'C
Cuts at: 3542 3542
Size: 6217
MfeI C'AATT_G
Cuts at: 3255 3816 3255
Size: 561 5656
MluI A'CGCG_T
Cuts at: 6093 6093
Size: 6217
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3108 5331 5515 1305
Size: 1803 2223 184 2007
Fragments arranged by size:
2223 2007 1803 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4395
Fragments arranged by size:
4395 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2057 2116 2165 2360 2670 4175 4534
Size: 648 891 59 49 195 310 1505 359
Cuts at: 4534 4693 518
Size: 159 2042
Fragments arranged by size:
2042 1505 891 648 359 310 195 159
59 49
MspA1I CmG'CkG
Cuts at: 1606 2984 3633 4310 5458 5703 1606
Size: 1378 649 677 1148 245 2120
Fragments arranged by size:
2120 1378 1148 677 649 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2058 3364 513
Size: 552 939 54 1306 3366
Fragments arranged by size:
3366 1306 939 552 54
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6217
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6217
NotI GC'GGCC_GC
Cuts at: 3664 3664
Size: 6217
NspI r_CATG'y
Cuts at: 1132 1229 3575 5120 1132
Size: 97 2346 1545 2229
Fragments arranged by size:
2346 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6217
PciI A'CATG_T
Cuts at: 5116 5116
Size: 6217
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2234 1108
Size: 1126 5091
PfoI T'CCnGG_A
Cuts at: 2812 2847 2812
Size: 35 6182
PmeI GTTT'AAAC
Cuts at: 3521 3521
Size: 6217
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4286 4318 5799 5831 4286
Size: 32 1481 32 4672
Fragments arranged by size:
4672 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2810 3109 2810
Size: 299 5918
PshAI GACnn'nnGTC
Cuts at: 1613 2347 1613
Size: 734 5483
PsiI TTA'TAA
Cuts at: 3787 3787
Size: 6217
PspXI vC'TCGA_Gb
Cuts at: 3513 3513
Size: 6217
PstI C_TGCA'G
Cuts at: 830 3163 3450 3569 830
Size: 2333 287 119 3478
Fragments arranged by size:
3478 2333 287 119
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6217
PvuII CAG'CTG
Cuts at: 2984 2984
Size: 6217
SacI G_AGCT'C
Cuts at: 2691 2769 2691
Size: 78 6139
SalI G'TCGA_C
Cuts at: 1936 3559 1936
Size: 1623 4594
SbfI CC_TGCA'GG
Cuts at: 3569 3569
Size: 6217
ScaI AGT'ACT
Cuts at: 2615 4465 6177 2615
Size: 1850 1712 2655
Fragments arranged by size:
2655 1850 1712
SfcI C'TryA_G
Cuts at: 826 1045 3159 3446 3565 4700 5381 5572 826
Size: 219 2114 287 119 1135 681 191 1471
Fragments arranged by size:
2114 1471 1135 681 287 219 191 119
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6217
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6217
SmaI CCC'GGG
Cuts at: 1872 3544 1872
Size: 1672 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2015 2303 3513 4321 5222 5484
Size: 197 926 72 288 1210 808 901 262
Cuts at: 5484 820
Size: 1553
Fragments arranged by size:
1553 1210 926 901 808 288 262 197
72
SnaBI TAC'GTA
Cuts at: 493 4008 493
Size: 3515 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6217
SphI G_CATG'C
Cuts at: 1132 3575 1132
Size: 2443 3774
SspI AAT'ATT
Cuts at: 5 52 4141 5
Size: 47 4089 2081
Fragments arranged by size:
4089 2081 47
StuI AGG'CCT
Cuts at: 1517 2013 1517
Size: 496 5721
StyI C'CwwG_G
Cuts at: 513 1065 2004 2058 2710 3112 3364 3656 513
Size: 552 939 54 652 402 252 292 3074
Fragments arranged by size:
3074 939 652 552 402 292 252 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4244 4261 4414 4599 5044 325
Size: 1255 2664 17 153 185 445 1498
Fragments arranged by size:
2664 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2613 4463 6175
Size: 274 80 33 51 1443 636 1850 1712
Cuts at: 6175 96
Size: 138
Fragments arranged by size:
1850 1712 1443 636 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2364 2396 2725 2757 2829 2861 3057 3089 6177
Size: 32 329 32 72 32 196 32 3088
Cuts at: 6177 6209 2364
Size: 32 2372
Fragments arranged by size:
3088 2372 329 196 72 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6217
XbaI T'CTAG_A
Cuts at: 3553 3553
Size: 6217
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2366 2366
Size: 6217
XhoI C'TCGA_G
Cuts at: 1943 3513 1943
Size: 1570 4647
XmaI C'CCGG_G
Cuts at: 1870 3542 1870
Size: 1672 4545
XmnI GAAnn'nnTTC
Cuts at: 2928 4346 2928
Size: 1418 4799
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4025 276
Size: 53 83 186 936 2491 2468
Fragments arranged by size:
2491 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI