Restriction map for
pFN21AE3218
(Circular) (Six-base) MAPSORT of: pFN21AE3218.seq Check: 9789 from: 1 to: 6190
pFN21AE3218 6190 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2109 2645 2887 3150 4253 5637 5905 1632
Size: 477 536 242 263 1103 1384 268 1917
Fragments arranged by size:
1917 1384 1103 536 477 268 263 242
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 5089 6066 1203
Size: 3886 977 1327
Fragments arranged by size:
3886 1327 977
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2336 2914 1184
Size: 1152 578 4460
Fragments arranged by size:
4460 1152 578
AlwNI CAG_nnn'CTG
Cuts at: 3933 5505 3933
Size: 1572 4618
ApaLI G'TGCA_C
Cuts at: 3269 4247 5403 3269
Size: 978 1156 4056
Fragments arranged by size:
4056 1156 978
ApoI r'AATT_y
Cuts at: 1493 2686 3499 3725 3877 5986 1493
Size: 1193 813 226 152 2109 1697
Fragments arranged by size:
2109 1697 1193 813 226 152
AscI GG'CGCG_CC
Cuts at: 3435 3435
Size: 6190
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2106 3429 3515 1870
Size: 73 163 1323 86 4545
Fragments arranged by size:
4545 1323 163 86 73
BamHI G'GATC_C
Cuts at: 2497 2847 3135 3520 3887 2497
Size: 350 288 385 367 4800
Fragments arranged by size:
4800 385 367 350 288
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2855 3511 4966 618
Size: 325 217 627 1068 656 1455 1842
Fragments arranged by size:
1842 1455 1068 656 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3428 3509 1391
Size: 2037 81 4072
Fragments arranged by size:
4072 2037 81
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2038 2097 2346 2633 928
Size: 971 139 59 249 287 4485
Fragments arranged by size:
4485 971 287 249 139 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4082 5292 4082
Size: 1210 4980
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6190
BfrBI ATG'CAT
Cuts at: 2378 2378
Size: 6190
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3383 4801 136
Size: 107 122 71 631 605 1711 1418 1525
Fragments arranged by size:
1711 1525 1418 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 3607 3607
Size: 6190
Bme1580I G_kGCm'C
Cuts at: 1790 3273 4251 5407 1790
Size: 1483 978 1156 2573
Fragments arranged by size:
2573 1483 1156 978
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3390 3921 4879
Size: 416 53 83 399 24 1973 531 958
Cuts at: 4879 442
Size: 1753
Fragments arranged by size:
1973 1753 958 531 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2315 2745 3346 4850 1239
Size: 1076 430 601 1504 2579
Fragments arranged by size:
2579 1504 1076 601 430
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2624 4315 5180 5478 2624
Size: 1691 865 298 3336
Fragments arranged by size:
3336 1691 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3238 3981 493
Size: 711 2034 743 2702
Fragments arranged by size:
2702 2034 743 711
BsaBI GATnn'nnATC
Cuts at: 6184 6184
Size: 6190
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3997 4379 5014 275
Size: 53 83 186 936 2464 382 635 1451
Fragments arranged by size:
2464 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2676 2844 4623 5021 5295 5442 5853 2676
Size: 168 1779 398 274 147 411 3013
Fragments arranged by size:
3013 1779 411 398 274 168 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3362 3392 3464 3494 1178
Size: 30 165 30 1959 30 72 30 3874
Fragments arranged by size:
3874 1959 165 72 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2176 3003 1186
Size: 990 827 4373
Fragments arranged by size:
4373 990 827
BseYI C'CCAG_C
Cuts at: 2177 2404 2697 2782 2991 3418 5393 5889 2177
Size: 227 293 85 209 427 1975 496 2478
Fragments arranged by size:
2478 1975 496 427 293 227 209 85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5617
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2521 3640 4401 5429 1540
Size: 275 186 520 1119 761 1028 2301
Fragments arranged by size:
2301 1119 1028 761 520 275 186
BsiHKAI G_wGCw'C
Cuts at: 3273 4251 4336 5407 3273
Size: 978 85 1071 4056
Fragments arranged by size:
4056 1071 978 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
BsmBI CGTCTCn'nnnn_
Cuts at: 2459 2459
Size: 6190
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3273 3428 3509 4251 4336 5407 1391
Size: 399 1483 155 81 742 85 1071 2174
Fragments arranged by size:
2174 1483 1071 742 399 155 85 81
BspEI T'CCGG_A
Cuts at: 2844 5853 2844
Size: 3009 3181
BspHI T'CATG_A
Cuts at: 3109 4079 5809 3109
Size: 970 1730 3490
Fragments arranged by size:
3490 1730 970
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2622 3264 3545 844
Size: 1778 642 281 3489
Fragments arranged by size:
3489 1778 642 281
BsrBI CCG'CTC
Cuts at: 1394 1823 2123 3036 4077 1394
Size: 429 300 913 1041 3507
Fragments arranged by size:
3507 1041 913 429 300
BsrDI GCAATG_nn'
Cuts at: 65 1248 4685 4859 65
Size: 1183 3437 174 1396
Fragments arranged by size:
3437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2556 2820 2955 4834 5021 1700
Size: 110 746 264 135 1879 187 2869
Fragments arranged by size:
2869 1879 746 264 187 135 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 3435 1925
Size: 1510 4680
BssSI C'ACGA_G
Cuts at: 2444 3272 4250 5262 2444
Size: 828 978 1012 3372
Fragments arranged by size:
3372 1012 978 828
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2835 794
Size: 2041 4149
BstBI TT'CG_AA
Cuts at: 2488 4994 2488
Size: 2506 3684
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2497 2847 3135 3520 3887 4272 4289 5741
Size: 525 350 288 385 367 385 17 1452
Cuts at: 5741 5827 5839 6185 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 525 385 385 367 350 346
288 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3200 3200
Size: 6190
BtgI C'CryG_G
Cuts at: 513 1065 2137 513
Size: 552 1072 4566
Fragments arranged by size:
4566 1072 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5451
Fragments arranged by size:
5451 683 56
BtsI GCAGTG_nn'
Cuts at: 3126 3303 3706 4500 4520 3126
Size: 177 403 794 20 4796
Fragments arranged by size:
4796 794 403 177 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 3494 3494
Size: 6190
DraIII CAC_nnn'GTG
Cuts at: 3213 3213
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5197 809
Size: 507 3881 1802
Fragments arranged by size:
3881 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2134 3637 4526
Size: 54 1425 50 275 18 304 1503 889
Cuts at: 4526 6155 8
Size: 1629 43
Fragments arranged by size:
1629 1503 1425 889 304 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3637 1537
Size: 275 1825 4090
Fragments arranged by size:
4090 1825 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2567 4120 1353
Size: 1214 1553 3423
Fragments arranged by size:
3423 1553 1214
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2494 4777 5151 5297 387
Size: 1064 138 905 2283 374 146 1280
Fragments arranged by size:
2283 1280 1064 905 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2109 2315 2645 2745 2887 3150 3346
Size: 393 477 206 330 100 142 263 196
Cuts at: 3346 4253 4850 5637 5905 1239
Size: 907 597 787 268 1524
Fragments arranged by size:
1524 907 787 597 477 393 330 268
263 206 196 142 100
EcoO109I rG'GnC_Cy
Cuts at: 2181 2926 3196 3466 2181
Size: 745 270 270 4905
Fragments arranged by size:
4905 745 270 270
EcoRI G'AATT_C
Cuts at: 2686 3499 3877 2686
Size: 813 378 4999
Fragments arranged by size:
4999 813 378
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 5337 1678
Size: 3659 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2135 2141 2167 2173 2514
Size: 32 135 32 563 6 26 6 341
Cuts at: 2514 2546 4831 4863 4905 4937 1373
Size: 32 2285 32 42 32 2626
Fragments arranged by size:
2626 2285 563 341 135 42 32 32
32 32 32 26 6 6
HincII GTy'rAC
Cuts at: 669 1657 1938 2160 2934 3534 3780 669
Size: 988 281 222 774 600 246 3079
Fragments arranged by size:
3079 988 774 600 281 246 222
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3789 3789
Size: 6190
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2566 5304 5488 1305
Size: 1261 2738 184 2007
Fragments arranged by size:
2738 2007 1261 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4368
Fragments arranged by size:
4368 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2142 3240 4148 4507 4666 518
Size: 648 976 1098 908 359 159 2042
Fragments arranged by size:
2042 1098 976 908 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2177 2404 2460 3606 4283 5431 5676 1606
Size: 571 227 56 1146 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 677 571 245 227 56
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5638
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 3637 3637
Size: 6190
NsiI A_TGCA'T
Cuts at: 2380 2380
Size: 6190
NspI r_CATG'y
Cuts at: 1132 1229 2833 3269 3548 5093 1132
Size: 97 1604 436 279 1545 2229
Fragments arranged by size:
2229 1604 1545 436 279 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 5089 5089
Size: 6190
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6190
PfoI T'CCnGG_A
Cuts at: 2331 2331
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PmlI CAC'GTG
Cuts at: 3238 3238
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2181 2926 3196 3466 2181
Size: 745 270 270 4905
Fragments arranged by size:
4905 745 270 270
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6190
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PstI C_TGCA'G
Cuts at: 830 2840 3542 830
Size: 2010 702 3478
Fragments arranged by size:
3478 2010 702
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
PvuII CAG'CTG
Cuts at: 2177 2404 2460 2177
Size: 227 56 5907
Fragments arranged by size:
5907 227 56
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 2567 2567
Size: 6190
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 4438 6150 4438
Size: 1712 4478
SfcI C'TryA_G
Cuts at: 826 1045 2836 3484 3538 4673 5354 5545 826
Size: 219 1791 648 54 1135 681 191 1471
Fragments arranged by size:
1791 1471 1135 681 648 219 191 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 2108 3431 3517 1872
Size: 236 1323 86 4545
Fragments arranged by size:
4545 1323 236 86
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2639 4294 5195 5457 820
Size: 197 926 696 1655 901 262 1553
Fragments arranged by size:
1655 1553 926 901 696 262 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 2833 3548 1132
Size: 1701 715 3774
Fragments arranged by size:
3774 1701 715
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2066 2508 3381 3629 513
Size: 552 1001 442 873 248 3074
Fragments arranged by size:
3074 1001 873 552 442 248
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2535 2823 4217 4234 4387 4572 5017
Size: 1255 955 288 1394 17 153 185 445
Cuts at: 5017 325
Size: 1498
Fragments arranged by size:
1498 1394 1255 955 445 288 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2906 4436 6148
Size: 274 80 33 51 1443 929 1530 1712
Cuts at: 6148 96
Size: 138
Fragments arranged by size:
1712 1530 1443 929 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2705 2737 2839 2871 2945 2962 2977 2994 3276
Size: 32 102 32 74 17 15 17 282
Cuts at: 3276 3308 6150 6182 2705
Size: 32 2842 32 2713
Fragments arranged by size:
2842 2713 282 102 74 32 32 32
32 17 17 15
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 2106 3429 3515 1870
Size: 236 1323 86 4545
Fragments arranged by size:
4545 1323 236 86
XmnI GAAnn'nnTTC
Cuts at: 4319 4319
Size: 6190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BstXI BstZ17I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI