Restriction map for
pFN21AE3213
(Circular) (Six-base) MAPSORT of: pFN21AE3213.seq Check: 618 from: 1 to: 6178
pFN21AE3213 6178 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2166 2166
Size: 6178
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3428 3521 1937
Size: 1491 93 4594
Fragments arranged by size:
4594 1491 93
Acc65I G'GTAC_C
Cuts at: 2040 3499 2040
Size: 1459 4719
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2129 2735 4241 5625 5893 1632
Size: 497 606 1506 1384 268 1917
Fragments arranged by size:
1917 1506 1384 606 497 268
AfeI AGC'GCT
Cuts at: 2087 2939 2087
Size: 852 5326
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 2804 1166
Size: 1638 4540
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AlwNI CAG_nnn'CTG
Cuts at: 3121 3921 5493 3121
Size: 800 1572 3806
Fragments arranged by size:
3806 1572 800
ApaI G_GGCC'C
Cuts at: 2757 2873 2757
Size: 116 6062
ApaLI G'TGCA_C
Cuts at: 2789 4235 5391 2789
Size: 1446 1156 3576
Fragments arranged by size:
3576 1446 1156
ApoI r'AATT_y
Cuts at: 1493 3487 3713 3865 5974 1493
Size: 1994 226 152 2109 1697
Fragments arranged by size:
2109 1994 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2443 2858 2941 3062 3503 1870
Size: 73 500 415 83 121 441 4545
Fragments arranged by size:
4545 500 441 415 121 83 73
BamHI G'GATC_C
Cuts at: 3508 3875 3508
Size: 367 5811
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2040 2840 3499 4954 618
Size: 325 217 627 253 800 659 1455 1842
Fragments arranged by size:
1842 1455 800 659 627 325 253 217
BanII G_rGCy'C
Cuts at: 1391 2681 2757 2859 2873 3497 1391
Size: 1290 76 102 14 624 4072
Fragments arranged by size:
4072 1290 624 102 76 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2138 2954 928
Size: 971 239 816 4152
Fragments arranged by size:
4152 971 816 239
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2314 4070 5280 2314
Size: 1756 1210 3212
Fragments arranged by size:
3212 1756 1210
BclI T'GATC_A
Cuts at: 1548 2775 2796 3259 1548
Size: 1227 21 463 4467
Fragments arranged by size:
4467 1227 463 21
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2838 4789 136
Size: 107 122 71 631 605 1166 1951 1525
Fragments arranged by size:
1951 1525 1166 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6173 6173
Size: 6178
BlpI GC'TnA_GC
Cuts at: 3595 3595
Size: 6178
Bme1580I G_kGCm'C
Cuts at: 1790 2757 2793 2873 4239 5395 1790
Size: 967 36 80 1366 1156 2573
Fragments arranged by size:
2573 1366 1156 967 80 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6178
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2526 3909 4867
Size: 416 53 83 399 24 1109 1383 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1753 1383 1109 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2713 2963 4838 1239
Size: 1474 250 1875 2579
Fragments arranged by size:
2579 1875 1474 250
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2489 3314 4303 5168 5466 2489
Size: 825 989 865 298 3201
Fragments arranged by size:
3201 989 865 825 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4841 882
Size: 617 3342 2219
Fragments arranged by size:
3342 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2794 3404 3969 493
Size: 711 1590 610 565 2702
Fragments arranged by size:
2702 1590 711 610 565
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3985 4367 5002 275
Size: 53 83 186 936 2452 382 635 1451
Fragments arranged by size:
2452 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5953
Fragments arranged by size:
5953 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3080 1186
Size: 1894 4284
BseYI C'CCAG_C
Cuts at: 5381 5877 5381
Size: 496 5682
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2746 3005 3197 1163
Size: 573 1010 259 192 4144
Fragments arranged by size:
4144 1010 573 259 192
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2396 3628 4389 5417 1540
Size: 275 186 395 1232 761 1028 2301
Fragments arranged by size:
2301 1232 1028 761 395 275 186
BsiHKAI G_wGCw'C
Cuts at: 2391 2793 4239 4324 5395 2391
Size: 402 1446 85 1071 3174
Fragments arranged by size:
3174 1446 1071 402 85
BsmI GAATG_Cn'
Cuts at: 2586 3345 3689 3782 2586
Size: 759 344 93 4982
Fragments arranged by size:
4982 759 344 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2391 2681 2757 2793 2859 2873 3497
Size: 399 601 290 76 36 66 14 624
Cuts at: 3497 4239 4324 5395 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 624 601 399 290 85
76 66 36 14
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 2190 3219 4067 5797 2190
Size: 1029 848 1730 2571
Fragments arranged by size:
2571 1730 1029 848
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2166 3006 3533 844
Size: 1322 840 527 3489
Fragments arranged by size:
3489 1322 840 527
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2391 4822 5009 1700
Size: 110 581 2431 187 2869
Fragments arranged by size:
2869 2431 581 187 110
BsrGI T'GTAC_A
Cuts at: 96 2967 3027 96
Size: 2871 60 3247
Fragments arranged by size:
3247 2871 60
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6178
BstBI TT'CG_AA
Cuts at: 4982 4982
Size: 6178
BstEII G'GTnAC_C
Cuts at: 1184 2946 1184
Size: 1762 4416
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 2743
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 3508 3875 4260 4277 5729 5815 5827 6173
Size: 1536 367 385 17 1452 86 12 346
Cuts at: 6173 1972
Size: 1977
Fragments arranged by size:
1977 1536 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2865 2865
Size: 6178
BtgI C'CryG_G
Cuts at: 513 1065 2004 2044 2682 513
Size: 552 939 40 638 4009
Fragments arranged by size:
4009 939 638 552 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 2758 3694 4488 4508 2758
Size: 936 794 20 4428
Fragments arranged by size:
4428 936 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 2274 3482 2274
Size: 1208 4970
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2393 2745 3625
Size: 54 1425 50 275 18 563 352 880
Cuts at: 3625 4514 6143 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 880 563 352 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2393 3625 1537
Size: 275 581 1232 4090
Fragments arranged by size:
4090 1232 581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2004 2136 2591 3048 4108 1353
Size: 651 132 455 457 1060 3423
Fragments arranged by size:
3423 1060 651 457 455 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4765 5139 5285 387
Size: 1064 138 3176 374 146 1280
Fragments arranged by size:
3176 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2129 2713 2735 2963 4241 4838 5625
Size: 393 497 584 22 228 1278 597 787
Cuts at: 5625 5893 1239
Size: 268 1524
Fragments arranged by size:
1524 1278 787 597 584 497 393 268
228 22
EcoO109I rG'GnC_Cy
Cuts at: 2314 2754 2869 2314
Size: 440 115 5623
Fragments arranged by size:
5623 440 115
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2740 2740
Size: 6178
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2089 2941 5325 1678
Size: 411 852 2384 2531
Fragments arranged by size:
2531 2384 852 411
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2546 2578 3239 3271 3415
Size: 32 135 32 974 32 661 32 144
Cuts at: 3415 3447 4819 4851 4893 4925 1373
Size: 32 1372 32 42 32 2626
Fragments arranged by size:
2626 1372 974 661 144 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3382 3429 3522 3768 669
Size: 988 281 1444 47 93 246 3079
Fragments arranged by size:
3079 1444 988 281 246 93 47
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6178
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KpnI G_GTAC'C
Cuts at: 2044 3503 2044
Size: 1459 4719
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2760 2973 5292 5476 1305
Size: 1455 213 2319 184 2007
Fragments arranged by size:
2319 2007 1455 213 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2747 10
Size: 54 1425 343 915 3441
Fragments arranged by size:
3441 1425 915 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2804 4136 4495 4654 518
Size: 648 1638 1332 359 159 2042
Fragments arranged by size:
2042 1638 1332 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3594 4271 5419 5664 1606
Size: 1988 677 1148 245 2120
Fragments arranged by size:
2120 1988 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4687
Fragments arranged by size:
4687 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 2494 3536 5081 1132
Size: 97 1265 1042 1545 2229
Fragments arranged by size:
2229 1545 1265 1042 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6178
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2426 1108
Size: 1318 4860
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PmlI CAC'GTG
Cuts at: 2794 2794
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2314 2314
Size: 6178
PshAI GACnn'nnGTC
Cuts at: 1613 2296 1613
Size: 683 5495
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PspOMI G'GGCC_C
Cuts at: 2753 2869 2753
Size: 116 6062
PstI C_TGCA'G
Cuts at: 830 2163 2610 3246 3530 830
Size: 1333 447 636 284 3478
Fragments arranged by size:
3478 1333 636 447 284
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3427 3520 1936
Size: 1491 93 4594
Fragments arranged by size:
4594 1491 93
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SfcI C'TryA_G
Cuts at: 826 1045 2159 2606 3242 3526 4661 5342 5533
Size: 219 1114 447 636 284 1135 681 191
Cuts at: 5533 826
Size: 1471
Fragments arranged by size:
1471 1135 1114 681 636 447 284 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 3505 1872
Size: 1633 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2443 2504 3329 4282 5183 5445
Size: 197 926 500 61 825 953 901 262
Cuts at: 5445 820
Size: 1553
Fragments arranged by size:
1553 953 926 901 825 500 262 197
61
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 2494 3536 1132
Size: 1362 1042 3774
Fragments arranged by size:
3774 1362 1042
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 3015 3268 1517
Size: 1498 253 4427
Fragments arranged by size:
4427 1498 253
StyI C'CwwG_G
Cuts at: 513 1065 2004 2917 3204 3617 513
Size: 552 939 913 287 413 3074
Fragments arranged by size:
3074 939 913 552 413 287
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4205 4222 4375 4560 5005 325
Size: 1255 2625 17 153 185 445 1498
Fragments arranged by size:
2625 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2967 3027 4424
Size: 274 80 33 51 1443 990 60 1397
Cuts at: 4424 6136 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1397 990 274 138 80 60
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2833 2865 6138 6170 2833
Size: 32 3273 32 2841
Fragments arranged by size:
3273 2841 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XhoI C'TCGA_G
Cuts at: 1943 2443 1943
Size: 500 5678
XmaI C'CCGG_G
Cuts at: 1870 3503 1870
Size: 1633 4545
XmnI GAAnn'nnTTC
Cuts at: 2720 3057 4307 2720
Size: 337 1250 4591
Fragments arranged by size:
4591 1250 337
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI