Restriction map for
pFN21AE3212
(Circular) (Six-base) MAPSORT of: pFN21AE3212.seq Check: 9576 from: 1 to: 6142
pFN21AE3212 6142 bp
With 182 enzymes: SgfI *
July 6, 2012 17:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3952 278
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 3463 3463
Size: 6142
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2614 4205 5589 5857 1632
Size: 982 1591 1384 268 1917
Fragments arranged by size:
1917 1591 1384 982 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 2949 5041 6018 1203
Size: 1746 2092 977 1327
Fragments arranged by size:
2092 1746 1327 977
AgeI A'CCGG_T
Cuts at: 4973 4973
Size: 6142
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 3885 5457 3885
Size: 1572 4570
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 3467 1870
Size: 73 1524 4545
Fragments arranged by size:
4545 1524 73
BamHI G'GATC_C
Cuts at: 2740 3472 3839 2740
Size: 732 367 5043
Fragments arranged by size:
5043 732 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2337 3463 4918 618
Size: 325 217 627 550 1126 1455 1842
Fragments arranged by size:
1842 1455 1126 627 550 325 217
BanII G_rGCy'C
Cuts at: 1391 2054 2934 2966 3461 1391
Size: 663 880 32 495 4072
Fragments arranged by size:
4072 880 663 495 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2586 2985 928
Size: 971 687 399 4085
Fragments arranged by size:
4085 971 687 399
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3149 3183 4333 4367 1903
Size: 34 1212 34 1150 34 3678
Fragments arranged by size:
3678 1212 1150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2819 4034 5244 2819
Size: 1215 1210 3717
Fragments arranged by size:
3717 1215 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4753 136
Size: 107 122 71 631 605 3081 1525
Fragments arranged by size:
3081 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3018 6137 3018
Size: 3119 3023
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2804 3375 4203 5359 1790
Size: 1014 571 828 1156 2573
Fragments arranged by size:
2573 1156 1014 828 571
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2740 3873 4831
Size: 416 53 83 399 24 1323 1133 958
Cuts at: 4831 442
Size: 1753
Fragments arranged by size:
1753 1323 1133 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2351 2433 2530 3398 4802 1239
Size: 848 264 82 97 868 1404 2579
Fragments arranged by size:
2579 1404 868 848 264 97 82
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2980 4267 5132 5430 2980
Size: 1287 865 298 3692
Fragments arranged by size:
3692 1287 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4805 882
Size: 617 3306 2219
Fragments arranged by size:
3306 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2952 2970 3933 493
Size: 711 1748 18 963 2702
Fragments arranged by size:
2702 1748 963 711 18
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3949 4331 4966 275
Size: 53 83 186 936 2416 382 635 1451
Fragments arranged by size:
2416 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2382 4575 4973 5247 5394 5805 2382
Size: 2193 398 274 147 411 2719
Fragments arranged by size:
2719 2193 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3044 3074 1178
Size: 30 165 30 1641 30 4246
Fragments arranged by size:
4246 1641 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3176 3383 1186
Size: 1990 207 3945
Fragments arranged by size:
3945 1990 207
BseYI C'CCAG_C
Cuts at: 5345 5841 5345
Size: 496 5646
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5569
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3592 4353 5381 1540
Size: 275 186 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2934 2966 4203 4288 5359 2934
Size: 32 1237 85 1071 3717
Fragments arranged by size:
3717 1237 1071 85 32
BsmI GAATG_Cn'
Cuts at: 3412 3653 3746 3412
Size: 241 93 5808
Fragments arranged by size:
5808 241 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2804 2934 2966 3375 3461 4203
Size: 399 264 750 130 32 409 86 742
Cuts at: 4203 4288 5359 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 750 742 409 399 264 130
86 85 32
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 3321 4031 5761 3321
Size: 710 1730 3702
Fragments arranged by size:
3702 1730 710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3112 4029 1394
Size: 429 188 1101 917 3507
Fragments arranged by size:
3507 1101 917 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4786 4973 1700
Size: 110 2976 187 2869
Fragments arranged by size:
2976 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6142
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2605 2605
Size: 6142
BstYI r'GATC_y
Cuts at: 1972 2740 3018 3472 3839 4224 4241 5693 5779
Size: 768 278 454 367 385 17 1452 86
Cuts at: 5779 5791 6137 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 768 454 385 367 346 278
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2518 513
Size: 552 939 514 4137
Fragments arranged by size:
4137 939 552 514
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5403
Fragments arranged by size:
5403 683 56
BtsI GCAGTG_nn'
Cuts at: 3042 3658 4452 4472 3042
Size: 616 794 20 4712
Fragments arranged by size:
4712 794 616 20
ClaI AT'CG_AT
Cuts at: 1554 2287 1554
Size: 733 5409
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2701 2871 3589
Size: 54 1425 50 275 18 871 170 718
Cuts at: 3589 4478 6107 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 871 718 275 170 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3589 1537
Size: 275 1777 4090
Fragments arranged by size:
4090 1777 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2490 4072 1353
Size: 1137 1582 3423
Fragments arranged by size:
3423 1582 1137
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2079 4729 5103 5249 387
Size: 1064 138 490 2650 374 146 1280
Fragments arranged by size:
2650 1280 1064 490 374 146 138
EcoICRI GAG'CTC
Cuts at: 2932 2964 2932
Size: 32 6110
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2351 2433 2530 2614 3398 4205
Size: 393 455 264 82 97 84 784 807
Cuts at: 4205 4802 5589 5857 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 807 787 784 597 455 393 268
264 97 84 82
EcoNI CCTnn'n_nnAGG
Cuts at: 2031 3398 2031
Size: 1367 4775
EcoO109I rG'GnC_Cy
Cuts at: 2785 2785
Size: 6142
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
EcoRV GAT'ATC
Cuts at: 2448 3205 2448
Size: 757 5385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 6142
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 5289 1678
Size: 3611 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2732 2764 4783 4815 4857
Size: 32 135 32 1160 32 2019 32 42
Cuts at: 4857 4889 1373
Size: 32 2626
Fragments arranged by size:
2626 2019 1160 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3486 3732 669
Size: 988 281 1548 246 3079
Fragments arranged by size:
3079 1548 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6142
HpaI GTT'AAC
Cuts at: 3732 3732
Size: 6142
KpnI G_GTAC'C
Cuts at: 3467 3467
Size: 6142
MfeI C'AATT_G
Cuts at: 3239 3741 3239
Size: 502 5640
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5256 5440 1305
Size: 3951 184 2007
Fragments arranged by size:
3951 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2703 10
Size: 54 1425 343 871 3449
Fragments arranged by size:
3449 1425 871 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2648 2807 4100 4459 4618 518
Size: 648 1482 159 1293 359 159 2042
Fragments arranged by size:
2042 1482 1293 648 359 159 159
MspA1I CmG'CkG
Cuts at: 1606 2770 3232 3385 3558 4235 5383 5628 1606
Size: 1164 462 153 173 677 1148 245 2120
Fragments arranged by size:
2120 1164 1148 677 462 245 173 153
NcoI C'CATG_G
Cuts at: 513 1065 2004 2518 513
Size: 552 939 514 4137
Fragments arranged by size:
4137 939 552 514
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 3589 3589
Size: 6142
NspI r_CATG'y
Cuts at: 1132 1229 2410 3500 5045 1132
Size: 97 1181 1090 1545 2229
Fragments arranged by size:
2229 1545 1181 1090 97
PasI CC'CwG_GG
Cuts at: 1348 2543 1348
Size: 1195 4947
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2508 1108
Size: 1400 4742
PfoI T'CCnGG_A
Cuts at: 2064 2064
Size: 6142
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PmlI CAC'GTG
Cuts at: 2952 2952
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2785 2785
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3494 830
Size: 2664 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6142
PvuII CAG'CTG
Cuts at: 2770 3232 3385 2770
Size: 462 153 5527
Fragments arranged by size:
5527 462 153
SacI G_AGCT'C
Cuts at: 2934 2966 2934
Size: 32 6110
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 2345 3053 4390 6102 2345
Size: 708 1337 1712 2385
Fragments arranged by size:
2385 1712 1337 708
SfcI C'TryA_G
Cuts at: 826 1045 2205 3490 4625 5306 5497 826
Size: 219 1160 1285 1135 681 191 1471
Fragments arranged by size:
1471 1285 1160 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2959 4246 5147 5409 820
Size: 197 926 1016 1287 901 262 1553
Fragments arranged by size:
1553 1287 1016 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 2410 3500 1132
Size: 1278 1090 3774
Fragments arranged by size:
3774 1278 1090
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2004 2518 3581 513
Size: 552 939 514 1063 3074
Fragments arranged by size:
3074 1063 939 552 514
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4169 4186 4339 4524 4969 325
Size: 1255 2589 17 153 185 445 1498
Fragments arranged by size:
2589 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2343 2640 3051
Size: 274 80 33 51 1443 366 297 411
Cuts at: 3051 4388 6100 96
Size: 1337 1712 138
Fragments arranged by size:
1712 1443 1337 411 366 297 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2640 2672 2817 2849 2936 2968 3348 3380 6102
Size: 32 145 32 87 32 380 32 2722
Cuts at: 6102 6134 2640
Size: 32 2648
Fragments arranged by size:
2722 2648 380 145 87 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6142
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2540 2540
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6142
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 2618 4271 2618
Size: 1653 4489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3950 276
Size: 53 83 186 936 2416 2468
Fragments arranged by size:
2468 2416 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI