Restriction map for
pFN21AE3208
(Circular) (Six-base) MAPSORT of: pFN21AE3208.seq Check: 1124 from: 1 to: 5776
pFN21AE3208 5776 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3586 278
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3119 1937
Size: 1182 4594
Acc65I G'GTAC_C
Cuts at: 3097 3097
Size: 5776
AclI AA'CG_TT
Cuts at: 1563 3903 4276 1563
Size: 2340 373 3063
Fragments arranged by size:
3063 2340 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3839 5223 5491 1632
Size: 2207 1384 268 1917
Fragments arranged by size:
2207 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5579
AflIII A'CryG_T
Cuts at: 1203 4675 5652 1203
Size: 3472 977 1327
Fragments arranged by size:
3472 1327 977
AgeI A'CCGG_T
Cuts at: 4607 4607
Size: 5776
AhdI GACnn_n'nnGTC
Cuts at: 4505 4505
Size: 5776
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5776
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5776
AlwNI CAG_nnn'CTG
Cuts at: 2081 2623 2755 3519 5091 2081
Size: 542 132 764 1572 2766
Fragments arranged by size:
2766 1572 764 542 132
ApaI G_GGCC'C
Cuts at: 2745 2745
Size: 5776
ApaLI G'TGCA_C
Cuts at: 3833 4989 3833
Size: 1156 4620
ApoI r'AATT_y
Cuts at: 1493 3085 3311 3463 5572 1493
Size: 1592 226 152 2109 1697
Fragments arranged by size:
2109 1697 1592 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4330 160
Size: 872 3298 1606
Fragments arranged by size:
3298 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
AvaI C'yCGr_G
Cuts at: 1870 1943 2192 2376 3101 1870
Size: 73 249 184 725 4545
Fragments arranged by size:
4545 725 249 184 73
BamHI G'GATC_C
Cuts at: 3106 3473 3106
Size: 367 5409
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2898 3097 4552 618
Size: 325 217 627 1111 199 1455 1842
Fragments arranged by size:
1842 1455 1111 627 325 217 199
BanII G_rGCy'C
Cuts at: 1391 2180 2319 2351 2745 3095 1391
Size: 789 139 32 394 350 4072
Fragments arranged by size:
4072 789 394 350 139 32
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3967 4001 1903
Size: 34 2030 34 3678
Fragments arranged by size:
3678 2030 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3668 4878 3668
Size: 1210 4566
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5776
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2236 2261
Size: 107 122 71 631 605 337 227 25
Cuts at: 2261 2414 4387 136
Size: 153 1973 1525
Fragments arranged by size:
1973 1525 631 605 337 227 153 122
107 71 25
BglII A'GATC_T
Cuts at: 5771 5771
Size: 5776
BlpI GC'TnA_GC
Cuts at: 2129 2515 3193 2129
Size: 386 678 4712
Fragments arranged by size:
4712 678 386
Bme1580I G_kGCm'C
Cuts at: 1790 2745 2810 3837 4993 1790
Size: 955 65 1027 1156 2573
Fragments arranged by size:
2573 1156 1027 955 65
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5776
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3072 3507 4465
Size: 416 53 83 399 24 1655 435 958
Cuts at: 4465 442
Size: 1753
Fragments arranged by size:
1753 1655 958 435 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2462 2585 2600 2645 2720 2732 2741 4436
Size: 1223 123 15 45 75 12 9 1695
Cuts at: 4436 1239
Size: 2579
Fragments arranged by size:
2579 1695 1223 123 75 45 15 12
9
Bpu10I CC'TnA_GC
Cuts at: 2777 2777
Size: 5776
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2306 3901 4766 5064 2306
Size: 1595 865 298 3018
Fragments arranged by size:
3018 1595 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4439 882
Size: 617 2940 2219
Fragments arranged by size:
2940 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2049 3567 493
Size: 711 845 1518 2702
Fragments arranged by size:
2702 1518 845 711
BsaBI GATnn'nnATC
Cuts at: 5770 5770
Size: 5776
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3583 3965 4600 275
Size: 53 83 186 936 2050 382 635 1451
Fragments arranged by size:
2050 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4209 4607 4881 5028 5439 4209
Size: 398 274 147 411 4546
Fragments arranged by size:
4546 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5551
Fragments arranged by size:
5551 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2426 2459 2519 2603 2654 2738 2920 3036
Size: 1240 33 60 84 51 84 182 116
Cuts at: 3036 1186
Size: 3926
Fragments arranged by size:
3926 1240 182 116 84 84 60 51
33
BseYI C'CCAG_C
Cuts at: 2133 4979 5475 2133
Size: 2846 496 2434
Fragments arranged by size:
2846 2434 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2218 2375 2386 2441 2822 2866 1163
Size: 573 482 157 11 55 381 44 4073
Fragments arranged by size:
4073 573 482 381 157 55 44 11
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3226 3987 5015 1540
Size: 275 186 1225 761 1028 2301
Fragments arranged by size:
2301 1225 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2054 2310 2385 2735 3837 3922 4993 2054
Size: 256 75 350 1102 85 1071 2837
Fragments arranged by size:
2837 1102 1071 350 256 85 75
BsmI GAATG_Cn'
Cuts at: 3287 3380 3287
Size: 93 5683
BsmBI CGTCTCn'nnnn_
Cuts at: 2621 2621
Size: 5776
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2054 2180 2310 2319 2351 2385 2735
Size: 399 264 126 130 9 32 34 350
Cuts at: 2735 2745 2810 3095 3837 3922 4993 1391
Size: 10 65 285 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 350 285 264 130
126 85 65 34 32 10 9
BspEI T'CCGG_A
Cuts at: 5439 5439
Size: 5776
BspHI T'CATG_A
Cuts at: 3665 5395 3665
Size: 1730 4046
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2232 3131 844
Size: 1388 899 3489
Fragments arranged by size:
3489 1388 899
BsrBI CCG'CTC
Cuts at: 1394 1823 2545 2728 3663 1394
Size: 429 722 183 935 3507
Fragments arranged by size:
3507 935 722 429 183
BsrDI GCAATG_nn'
Cuts at: 65 1248 4271 4445 65
Size: 1183 3023 174 1396
Fragments arranged by size:
3023 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2235 2385 2682 4420 4607 1700
Size: 110 425 150 297 1738 187 2869
Fragments arranged by size:
2869 1738 425 297 187 150 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5776
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5776
BssSI C'ACGA_G
Cuts at: 2312 3836 4848 2312
Size: 1524 1012 3240
Fragments arranged by size:
3240 1524 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2389 2398 794
Size: 1595 9 4172
Fragments arranged by size:
4172 1595 9
BstBI TT'CG_AA
Cuts at: 4580 4580
Size: 5776
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5776
BstXI CCAn_nnnn'nTGG
Cuts at: 2062 2359 2062
Size: 297 5479
BstYI r'GATC_y
Cuts at: 1972 3106 3473 3858 3875 5327 5413 5425 5771
Size: 1134 367 385 17 1452 86 12 346
Cuts at: 5771 1972
Size: 1977
Fragments arranged by size:
1977 1452 1134 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5037
Fragments arranged by size:
5037 683 56
BtsI GCAGTG_nn'
Cuts at: 2424 2810 2988 3292 4086 4106 2424
Size: 386 178 304 794 20 4094
Fragments arranged by size:
4094 794 386 304 178 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5776
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5741
DraI TTT'AAA
Cuts at: 3080 3080
Size: 5776
DraIII CAC_nnn'GTG
Cuts at: 2398 2398
Size: 5776
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4783 809
Size: 507 3467 1802
Fragments arranged by size:
3467 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2253 3223
Size: 54 1425 50 275 18 268 155 970
Cuts at: 3223 4112 5741 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 970 889 275 268 155 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3223 1537
Size: 275 1411 4090
Fragments arranged by size:
4090 1411 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2595 2631 2973 3706 1353
Size: 1242 36 342 733 3423
Fragments arranged by size:
3423 1242 733 342 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2179 4363 4737 4883 387
Size: 1064 138 590 2184 374 146 1280
Fragments arranged by size:
2184 1280 1064 590 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2462 2585 2600 2645 2720 2732 2741
Size: 393 830 123 15 45 75 12 9
Cuts at: 2741 3839 4436 5223 5491 1239
Size: 1098 597 787 268 1524
Fragments arranged by size:
1524 1098 830 787 597 393 268 123
75 45 15 12 9
EcoNI CCTnn'n_nnAGG
Cuts at: 2300 3051 2300
Size: 751 5025
EcoO109I rG'GnC_Cy
Cuts at: 2741 2742 2741
Size: 1 5775
EcoRI G'AATT_C
Cuts at: 3085 3463 3085
Size: 378 5398
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2197 2197
Size: 5776
FspI TGC'GCA
Cuts at: 4282 4282
Size: 5776
HaeII r_GCGC'y
Cuts at: 1678 2958 4923 1678
Size: 1280 1965 2531
Fragments arranged by size:
2531 1965 1280
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2106 2138 2473 2505 2896
Size: 32 135 32 534 32 335 32 391
Cuts at: 2896 2928 4417 4449 4491 4523 1373
Size: 32 1489 32 42 32 2626
Fragments arranged by size:
2626 1489 534 391 335 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3120 3366 669
Size: 988 281 1182 246 3079
Fragments arranged by size:
3079 1182 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2324 748
Size: 1576 4200
HpaI GTT'AAC
Cuts at: 3366 3366
Size: 5776
KpnI G_GTAC'C
Cuts at: 3101 3101
Size: 5776
MfeI C'AATT_G
Cuts at: 3375 3375
Size: 5776
MluI A'CGCG_T
Cuts at: 5652 5652
Size: 5776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4890 5074 1305
Size: 3585 184 2007
Fragments arranged by size:
3585 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 10
Size: 54 1425 343 268 3686
Fragments arranged by size:
3686 1425 343 268 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3734 4093 4252 518
Size: 648 1191 1377 359 159 2042
Fragments arranged by size:
2042 1377 1191 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2719 3192 3869 5017 5262 1606
Size: 522 591 473 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 591 522 473 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4285
Fragments arranged by size:
4285 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5776
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5776
NotI GC'GGCC_GC
Cuts at: 3223 3223
Size: 5776
NspI r_CATG'y
Cuts at: 1132 1229 3134 4679 1132
Size: 97 1905 1545 2229
Fragments arranged by size:
2229 1905 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5776
PciI A'CATG_T
Cuts at: 4675 4675
Size: 5776
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 1108
Size: 1515 4261
PfoI T'CCnGG_A
Cuts at: 2658 2854 2658
Size: 196 5580
PmeI GTTT'AAAC
Cuts at: 3080 3080
Size: 5776
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3845 3877 5358 5390 3845
Size: 32 1481 32 4231
Fragments arranged by size:
4231 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5776
PsiI TTA'TAA
Cuts at: 3346 3346
Size: 5776
PspOMI G'GGCC_C
Cuts at: 2741 2741
Size: 5776
PstI C_TGCA'G
Cuts at: 830 2037 2286 2496 2718 2760 2995 3128 830
Size: 1207 249 210 222 42 235 133 3478
Fragments arranged by size:
3478 1207 249 235 222 210 133 42
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5776
PvuII CAG'CTG
Cuts at: 2128 2719 2128
Size: 591 5185
SalI G'TCGA_C
Cuts at: 1936 3118 1936
Size: 1182 4594
SapI GCTCTTCn'nnn_
Cuts at: 2595 2973 2595
Size: 378 5398
SbfI CC_TGCA'GG
Cuts at: 3128 3128
Size: 5776
ScaI AGT'ACT
Cuts at: 4024 5736 4024
Size: 1712 4064
SexAI A'CCwGG_T
Cuts at: 2589 2589
Size: 5776
SfcI C'TryA_G
Cuts at: 826 1045 2033 2282 2492 2714 2756 2991 3124
Size: 219 988 249 210 222 42 235 133
Cuts at: 3124 4259 4940 5131 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 988 681 249 235 222 219
210 191 133 42
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5776
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5776
SmaI CCC'GGG
Cuts at: 1872 2378 3103 1872
Size: 506 725 4545
Fragments arranged by size:
4545 725 506
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2321 3880 4781 5043 820
Size: 197 926 378 1559 901 262 1553
Fragments arranged by size:
1559 1553 926 901 378 262 197
SnaBI TAC'GTA
Cuts at: 493 3567 493
Size: 3074 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5776
SphI G_CATG'C
Cuts at: 1132 3134 1132
Size: 2002 3774
SspI AAT'ATT
Cuts at: 5 52 3700 5
Size: 47 3648 2081
Fragments arranged by size:
3648 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5776
StyI C'CwwG_G
Cuts at: 513 1065 2004 2247 3215 513
Size: 552 939 243 968 3074
Fragments arranged by size:
3074 968 939 552 243
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3017 3803 3820 3973 4158 4603 325
Size: 1255 1437 786 17 153 185 445 1498
Fragments arranged by size:
1498 1437 1255 786 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4022 5734 96
Size: 274 80 33 51 1443 2045 1712 138
Fragments arranged by size:
2045 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2033 2065 5736 5768 2033
Size: 32 3671 32 2041
Fragments arranged by size:
3671 2041 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5776
XbaI T'CTAG_A
Cuts at: 2884 3112 2884
Size: 228 5548
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5776
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5776
XmaI C'CCGG_G
Cuts at: 1870 2376 3101 1870
Size: 506 725 4545
Fragments arranged by size:
4545 725 506
XmnI GAAnn'nnTTC
Cuts at: 3905 3905
Size: 5776
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3584 276
Size: 53 83 186 936 2050 2468
Fragments arranged by size:
2468 2050 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI