Restriction map for
pFN21AE3205
(Circular) (Six-base) MAPSORT of: pFN21AE3205.seq Check: 1745 from: 1 to: 6133
pFN21AE3205 6133 bp
With 182 enzymes: SgfI *
March 28, 2012 14:20 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3943 278
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3476 1937
Size: 1539 4594
Acc65I G'GTAC_C
Cuts at: 3454 3454
Size: 6133
AclI AA'CG_TT
Cuts at: 1563 4260 4633 1563
Size: 2697 373 3063
Fragments arranged by size:
3063 2697 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2002 2306 2467 4196 5580 5848 1632
Size: 370 304 161 1729 1384 268 1917
Fragments arranged by size:
1917 1729 1384 370 304 268 161
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5936
AflIII A'CryG_T
Cuts at: 1203 2970 5032 6009 1203
Size: 1767 2062 977 1327
Fragments arranged by size:
2062 1767 1327 977
AgeI A'CCGG_T
Cuts at: 4964 4964
Size: 6133
AhdI GACnn_n'nnGTC
Cuts at: 4862 4862
Size: 6133
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6133
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2957 2989 2957
Size: 32 6101
AlwNI CAG_nnn'CTG
Cuts at: 2232 2335 2408 2551 3085 3130 3325 3876 5448
Size: 103 73 143 534 45 195 551 1572
Cuts at: 5448 2232
Size: 2917
Fragments arranged by size:
2917 1572 551 534 195 143 103 73
45
ApaLI G'TGCA_C
Cuts at: 2447 4190 5346 2447
Size: 1743 1156 3234
Fragments arranged by size:
3234 1743 1156
ApoI r'AATT_y
Cuts at: 1493 3442 3668 3820 5929 1493
Size: 1949 226 152 2109 1697
Fragments arranged by size:
2109 1949 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4687 160
Size: 872 3655 1606
Fragments arranged by size:
3655 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
AvaI C'yCGr_G
Cuts at: 1870 1943 2950 2988 3299 3458 1870
Size: 73 1007 38 311 159 4545
Fragments arranged by size:
4545 1007 311 159 73 38
BamHI G'GATC_C
Cuts at: 3463 3830 3463
Size: 367 5766
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2317 2909 3295 3454 4909
Size: 325 217 627 530 592 386 159 1455
Cuts at: 4909 618
Size: 1842
Fragments arranged by size:
1842 1455 627 592 530 386 325 217
159
BanII G_rGCy'C
Cuts at: 1391 2666 2928 2951 2997 3252 3452 1391
Size: 1275 262 23 46 255 200 4072
Fragments arranged by size:
4072 1275 262 255 200 46 23
BbeI G_GCGC'C
Cuts at: 3299 3299
Size: 6133
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2449 2606 928
Size: 971 550 157 4455
Fragments arranged by size:
4455 971 550 157
BbvCI CC'TCA_GC
Cuts at: 3015 3291 3015
Size: 276 5857
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3138 3172 4324 4358 1903
Size: 34 1201 34 1152 34 3678
Fragments arranged by size:
3678 1201 1152 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4025 5235 4025
Size: 1210 4923
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6133
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3195 3207 4744
Size: 107 122 71 631 605 1523 12 1537
Cuts at: 4744 136
Size: 1525
Fragments arranged by size:
1537 1525 1523 631 605 122 107 71
12
BglII A'GATC_T
Cuts at: 2715 6128 2715
Size: 3413 2720
BlpI GC'TnA_GC
Cuts at: 3550 3550
Size: 6133
Bme1580I G_kGCm'C
Cuts at: 1790 2451 3350 4194 5350 1790
Size: 661 899 844 1156 2573
Fragments arranged by size:
2573 1156 899 844 661
BmgBI CAC'GTC
Cuts at: 1318 2725 1318
Size: 1407 4726
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2316 3864 4822
Size: 416 53 83 399 24 899 1548 958
Cuts at: 4822 442
Size: 1753
Fragments arranged by size:
1753 1548 958 899 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6133
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2878 3269 2878
Size: 391 5742
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2513 2749 2867 2984 3086 3206 3239
Size: 968 306 236 118 117 102 120 33
Cuts at: 3239 4793 1239
Size: 1554 2579
Fragments arranged by size:
2579 1554 968 306 236 120 118 117
102 33
Bpu10I CC'TnA_GC
Cuts at: 3015 3291 3015
Size: 276 5857
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2458 4258 5123 5421 2458
Size: 1800 865 298 3170
Fragments arranged by size:
3170 1800 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2206 2243 2356 4796 882
Size: 617 707 37 113 2440 2219
Fragments arranged by size:
2440 2219 707 617 113 37
BsaAI yAC'GTr
Cuts at: 493 1204 2971 3172 3262 3924 493
Size: 711 1767 201 90 662 2702
Fragments arranged by size:
2702 1767 711 662 201 90
BsaBI GATnn'nnATC
Cuts at: 6127 6127
Size: 6133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2859 3296 3940
Size: 53 83 186 936 476 850 437 644
Cuts at: 3940 4322 4957 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 850 644 635 476 437 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3426 4566 4964 5238 5385 5796 3426
Size: 1140 398 274 147 411 3763
Fragments arranged by size:
3763 1140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2957 2987 3257 3287 1178
Size: 30 165 30 1554 30 270 30 4024
Fragments arranged by size:
4024 1554 270 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2294 2936 3242 1186
Size: 1108 642 306 4077
Fragments arranged by size:
4077 1108 642 306
BseYI C'CCAG_C
Cuts at: 2265 2688 3239 5336 5832 2265
Size: 423 551 2097 496 2566
Fragments arranged by size:
2566 2097 551 496 423
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2245 2364 2923 3074 3155 3384 1163
Size: 573 509 119 559 151 81 229 3912
Fragments arranged by size:
3912 573 559 509 229 151 119 81
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3583 4344 5372 1540
Size: 275 186 1582 761 1028 2301
Fragments arranged by size:
2301 1582 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2451 2793 2970 2997 3252 4194 4279 5350 2451
Size: 342 177 27 255 942 85 1071 3234
Fragments arranged by size:
3234 1071 942 342 255 177 85 27
BsiWI C'GTAC_G
Cuts at: 3105 3105
Size: 6133
BsmI GAATG_Cn'
Cuts at: 3644 3737 3644
Size: 93 6040
BsmBI CGTCTCn'nnnn_
Cuts at: 2070 2866 2070
Size: 796 5337
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2451 2666 2793 2928 2951 2970 2997
Size: 399 661 215 127 135 23 19 27
Cuts at: 2997 3252 3350 3452 4194 4279 5350 1391
Size: 255 98 102 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 661 399 255 215 135
127 102 98 85 27 23 19
BspEI T'CCGG_A
Cuts at: 5796 5796
Size: 6133
BspHI T'CATG_A
Cuts at: 4022 5752 4022
Size: 1730 4403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2644 3356 3488 844
Size: 1800 712 132 3489
Fragments arranged by size:
3489 1800 712 132
BsrBI CCG'CTC
Cuts at: 1394 1823 4020 1394
Size: 429 2197 3507
Fragments arranged by size:
3507 2197 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3105 4628 4802 65
Size: 1183 1857 1523 174 1396
Fragments arranged by size:
1857 1523 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4777 4964 1700
Size: 110 2967 187 2869
Fragments arranged by size:
2967 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3327 96
Size: 3231 2902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6133
BssSI C'ACGA_G
Cuts at: 2276 4193 5205 2276
Size: 1917 1012 3204
Fragments arranged by size:
3204 1917 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2232 794
Size: 1438 4695
BstBI TT'CG_AA
Cuts at: 4937 4937
Size: 6133
BstEII G'GTnAC_C
Cuts at: 1184 3019 1184
Size: 1835 4298
BstYI r'GATC_y
Cuts at: 1972 2583 2715 3463 3830 4215 4232 5684 5770
Size: 611 132 748 367 385 17 1452 86
Cuts at: 5770 5782 6128 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 748 611 385 367 346 132
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3035 3193 3035
Size: 158 5975
BtgI C'CryG_G
Cuts at: 513 1065 2004 3396 513
Size: 552 939 1392 3250
Fragments arranged by size:
3250 1392 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2355 487
Size: 683 56 1129 4265
Fragments arranged by size:
4265 1129 683 56
BtsI GCAGTG_nn'
Cuts at: 3649 4443 4463 3649
Size: 794 20 5319
Fragments arranged by size:
5319 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6133
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6098
DraI TTT'AAA
Cuts at: 2434 3437 2434
Size: 1003 5130
DraIII CAC_nnn'GTG
Cuts at: 3172 3397 3172
Size: 225 5908
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5140 809
Size: 507 3824 1802
Fragments arranged by size:
3824 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2118 3580 4469
Size: 54 1425 50 275 18 288 1462 889
Cuts at: 4469 6098 8
Size: 1629 43
Fragments arranged by size:
1629 1462 1425 889 288 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3580 1537
Size: 275 1768 4090
Fragments arranged by size:
4090 1768 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2847 4063 1353
Size: 1494 1216 3423
Fragments arranged by size:
3423 1494 1216
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4720 5094 5240 387
Size: 1064 138 3131 374 146 1280
Fragments arranged by size:
3131 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2995 3250 2995
Size: 255 5878
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2002 2207 2306 2467 2513 2749 2867
Size: 393 370 205 99 161 46 236 118
Cuts at: 2867 2984 3086 3206 3239 4196 4793 5580 5848
Size: 117 102 120 33 957 597 787 268
Cuts at: 5848 1239
Size: 1524
Fragments arranged by size:
1524 957 787 597 393 370 268 236
205 161 120 118 117 102 99 46
33
EcoNI CCTnn'n_nnAGG
Cuts at: 2473 2672 2473
Size: 199 5934
EcoO109I rG'GnC_Cy
Cuts at: 2175 3226 2175
Size: 1051 5082
EcoRI G'AATT_C
Cuts at: 3442 3820 3442
Size: 378 5755
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 6133
FspI TGC'GCA
Cuts at: 4639 4639
Size: 6133
HaeII r_GCGC'y
Cuts at: 1678 2030 2552 3157 3299 5280 1678
Size: 352 522 605 142 1981 2531
Fragments arranged by size:
2531 1981 605 522 352 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4774 4806 4848 4880 1373
Size: 32 135 32 3202 32 42 32 2626
Fragments arranged by size:
3202 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3477 3723 669
Size: 988 281 1539 246 3079
Fragments arranged by size:
3079 1539 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6133
HpaI GTT'AAC
Cuts at: 3723 3723
Size: 6133
KasI G'GCGC_C
Cuts at: 3295 3295
Size: 6133
KpnI G_GTAC'C
Cuts at: 3458 3458
Size: 6133
MfeI C'AATT_G
Cuts at: 3732 3732
Size: 6133
MluI A'CGCG_T
Cuts at: 6009 6009
Size: 6133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5247 5431 1305
Size: 3942 184 2007
Fragments arranged by size:
3942 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2120 10
Size: 54 1425 343 288 4023
Fragments arranged by size:
4023 1425 343 288 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 4091 4450 4609 518
Size: 648 1566 1359 359 159 2042
Fragments arranged by size:
2042 1566 1359 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2022 2035 2229 2392 2907 3549 4226 5374
Size: 416 13 194 163 515 642 677 1148
Cuts at: 5374 5619 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 642 515 416 245 194
163 13
NarI GG'CG_CC
Cuts at: 3296 3296
Size: 6133
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4642
Fragments arranged by size:
4642 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6133
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6133
NotI GC'GGCC_GC
Cuts at: 3580 3580
Size: 6133
NspI r_CATG'y
Cuts at: 1132 1229 3491 5036 1132
Size: 97 2262 1545 2229
Fragments arranged by size:
2262 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6133
PciI A'CATG_T
Cuts at: 5032 5032
Size: 6133
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3130 3172 3262 3397 1108
Size: 2022 42 90 135 3844
Fragments arranged by size:
3844 2022 135 90 42
PfoI T'CCnGG_A
Cuts at: 2490 3051 2490
Size: 561 5572
PmeI GTTT'AAAC
Cuts at: 3437 3437
Size: 6133
PmlI CAC'GTG
Cuts at: 2971 3262 2971
Size: 291 5842
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2957 2989 4202 4234 5715 5747 2957
Size: 32 1213 32 1481 32 3343
Fragments arranged by size:
3343 1481 1213 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2175 2175
Size: 6133
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6133
PsiI TTA'TAA
Cuts at: 3703 3703
Size: 6133
PstI C_TGCA'G
Cuts at: 830 2034 2543 2920 3345 3485 830
Size: 1204 509 377 425 140 3478
Fragments arranged by size:
3478 1204 509 425 377 140
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6133
PvuII CAG'CTG
Cuts at: 2035 2392 2035
Size: 357 5776
SacI G_AGCT'C
Cuts at: 2997 3252 2997
Size: 255 5878
SalI G'TCGA_C
Cuts at: 1936 3475 1936
Size: 1539 4594
SanDI GG'GwC_CC
Cuts at: 2175 2175
Size: 6133
SbfI CC_TGCA'GG
Cuts at: 3485 3485
Size: 6133
ScaI AGT'ACT
Cuts at: 4381 6093 4381
Size: 1712 4421
SexAI A'CCwGG_T
Cuts at: 3129 3129
Size: 6133
SfcI C'TryA_G
Cuts at: 826 1045 2030 2400 2539 2916 3341 3481 4616
Size: 219 985 370 139 377 425 140 1135
Cuts at: 4616 5297 5488 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 985 681 425 377 370 219
191 140 139
SfoI GGC'GCC
Cuts at: 3297 3297
Size: 6133
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6133
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6133
SmaI CCC'GGG
Cuts at: 1872 3460 1872
Size: 1588 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2473 4237 5138 5400 820
Size: 197 926 530 1764 901 262 1553
Fragments arranged by size:
1764 1553 926 901 530 262 197
SnaBI TAC'GTA
Cuts at: 493 3924 493
Size: 3431 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6133
SphI G_CATG'C
Cuts at: 1132 3491 1132
Size: 2359 3774
SspI AAT'ATT
Cuts at: 5 52 4057 5
Size: 47 4005 2081
Fragments arranged by size:
4005 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6133
StyI C'CwwG_G
Cuts at: 513 1065 2004 2196 3572 513
Size: 552 939 192 1376 3074
Fragments arranged by size:
3074 1376 939 552 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2279 2335 2338 4160 4177 4330 4515
Size: 1255 699 56 3 1822 17 153 185
Cuts at: 4515 4960 325
Size: 445 1498
Fragments arranged by size:
1822 1498 1255 699 445 185 153 56
17 3
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3327 4379 6091
Size: 274 80 33 51 1443 1350 1052 1712
Cuts at: 6091 96
Size: 138
Fragments arranged by size:
1712 1443 1350 1052 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2100 2132 2885 2917 2994 3026 3344 3376 6093
Size: 32 753 32 77 32 318 32 2717
Cuts at: 6093 6125 2100
Size: 32 2108
Fragments arranged by size:
2717 2108 753 318 77 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2813 1316
Size: 1497 4636
XbaI T'CTAG_A
Cuts at: 2296 2363 3469 2296
Size: 67 1106 4960
Fragments arranged by size:
4960 1106 67
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3415 3415
Size: 6133
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6133
XmaI C'CCGG_G
Cuts at: 1870 3458 1870
Size: 1588 4545
XmnI GAAnn'nnTTC
Cuts at: 4262 4262
Size: 6133
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3941 276
Size: 53 83 186 936 2407 2468
Fragments arranged by size:
2468 2407 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BstXI
BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacII SapI SfiI
SrfI SwaI