Restriction map for
pFN21AE3202
(Circular) (Six-base) MAPSORT of: pFN21AE3202.seq Check: 3644 from: 1 to: 6118
pFN21AE3202 6118 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2727 3461 1937
Size: 790 734 4594
Fragments arranged by size:
4594 790 734
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2921 3066 4181 5565 5833 1632
Size: 731 558 145 1115 1384 268 1917
Fragments arranged by size:
1917 1384 1115 731 558 268 145
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 2135 3284 1166
Size: 969 1149 4000
Fragments arranged by size:
4000 1149 969
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2099 1184
Size: 915 5203
AlwNI CAG_nnn'CTG
Cuts at: 3127 3861 5433 3127
Size: 734 1572 3812
Fragments arranged by size:
3812 1572 734
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2279 3050 3427 3653 3805 5914 1493
Size: 786 771 377 226 152 2109 1697
Fragments arranged by size:
2109 1697 786 771 377 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 3375 3443 1870
Size: 73 217 1215 68 4545
Fragments arranged by size:
4545 1215 217 73 68
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3198 3439 4894 618
Size: 325 217 627 1411 241 1455 1842
Fragments arranged by size:
1842 1455 1411 627 325 241 217
BanII G_rGCy'C
Cuts at: 1391 2028 2102 3437 1391
Size: 637 74 1335 4072
Fragments arranged by size:
4072 1335 637 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2583 928
Size: 971 684 4463
Fragments arranged by size:
4463 971 684
BbvCI CC'TCA_GC
Cuts at: 2943 2943
Size: 6118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4309 4343 1903
Size: 34 2372 34 3678
Fragments arranged by size:
3678 2372 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3391 4010 5220 3391
Size: 619 1210 4289
Fragments arranged by size:
4289 1210 619
BclI T'GATC_A
Cuts at: 1548 2139 1548
Size: 591 5527
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2781 4729 136
Size: 107 122 71 631 605 1109 1948 1525
Fragments arranged by size:
1948 1525 1109 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2049 2661 6113 2049
Size: 612 3452 2054
Fragments arranged by size:
3452 2054 612
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2678 3257 4179 5335 1790
Size: 888 579 922 1156 2573
Fragments arranged by size:
2573 1156 922 888 579
BmgBI CAC'GTC
Cuts at: 1318 2671 1318
Size: 1353 4765
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2107 3849 4807
Size: 416 53 83 399 24 690 1742 958
Cuts at: 4807 442
Size: 1753
Fragments arranged by size:
1753 1742 958 690 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1995 3149 4778 1239
Size: 756 1154 1629 2579
Fragments arranged by size:
2579 1629 1154 756
Bpu10I CC'TnA_GC
Cuts at: 2943 2943
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3233 4243 5108 5406 3233
Size: 1010 865 298 3945
Fragments arranged by size:
3945 1010 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3057 4781 882
Size: 617 1558 1724 2219
Fragments arranged by size:
2219 1724 1558 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 3075 6112 3075
Size: 3037 3081
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3925 4307 4942 275
Size: 53 83 186 936 2392 382 635 1451
Fragments arranged by size:
2392 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5893
Fragments arranged by size:
5893 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3023 1186
Size: 1837 4281
BseYI C'CCAG_C
Cuts at: 3406 5321 5817 3406
Size: 1915 496 3707
Fragments arranged by size:
3707 1915 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2720 3245 1163
Size: 573 984 525 4036
Fragments arranged by size:
4036 984 573 525
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2028 3273 4179 4264 5335 2028
Size: 1245 906 85 1071 2811
Fragments arranged by size:
2811 1245 1071 906 85
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2028 2102 2678 3257 3273 3437 4179
Size: 399 238 74 576 579 16 164 742
Cuts at: 4179 4264 5335 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 579 576 399 238 164
85 74 16
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 2736 4007 5737 2736
Size: 1271 1730 3117
Fragments arranged by size:
3117 1730 1271
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4613 4787 65
Size: 1183 3365 174 1396
Fragments arranged by size:
3365 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6118
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3268 794
Size: 2474 3644
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 2049 2661 3112 3448 3815 4200 4217 5669
Size: 77 612 451 336 367 385 17 1452
Cuts at: 5669 5755 5767 6113 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 612 451 385 367 346 336
86 77 17 12
Bsu36I CC'TnA_GG
Cuts at: 3384 3384
Size: 6118
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2330 2975 3056 3634 4428 4448 2265
Size: 65 645 81 578 794 20 3935
Fragments arranged by size:
3935 794 645 578 81 65 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3422 3422
Size: 6118
DraIII CAC_nnn'GTG
Cuts at: 3268 3268
Size: 6118
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2865 3565 4454
Size: 54 1425 50 275 18 1035 700 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1035 889 700 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2918 4048 1353
Size: 1565 1130 3423
Fragments arranged by size:
3423 1565 1130
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2026 2026
Size: 6118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 1995 2363 2921 3066 3149 4181 4778
Size: 393 363 368 558 145 83 1032 597
Cuts at: 4778 5565 5833 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1032 787 597 558 393 368 363
268 145 83
EcoO109I rG'GnC_Cy
Cuts at: 2947 2977 2947
Size: 30 6088
EcoRI G'AATT_C
Cuts at: 2279 3427 3805 2279
Size: 1148 378 4592
Fragments arranged by size:
4592 1148 378
EcoRV GAT'ATC
Cuts at: 2198 2198
Size: 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2546 2546
Size: 6118
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 5265 1678
Size: 3587 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2796 2828 4759 4791 4833
Size: 32 135 32 1224 32 1931 32 42
Cuts at: 4833 4865 1373
Size: 32 2626
Fragments arranged by size:
2626 1931 1224 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2611 3462 3708 669
Size: 988 281 673 851 246 3079
Fragments arranged by size:
3079 988 851 673 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2867 10
Size: 54 1425 343 1035 3261
Fragments arranged by size:
3261 1425 1035 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3284 4076 4435 4594 518
Size: 648 969 1149 792 359 159 2042
Fragments arranged by size:
2042 1149 969 792 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2070 2942 3534 4211 5359 5604 1606
Size: 464 872 592 677 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 592 464 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5566
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6118
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2884 2950 1348
Size: 1536 66 4516
Fragments arranged by size:
4516 1536 66
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2623 1108
Size: 1515 4603
PfoI T'CCnGG_A
Cuts at: 3030 3216 3030
Size: 186 5932
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspXI vC'TCGA_Gb
Cuts at: 2160 2160
Size: 6118
PstI C_TGCA'G
Cuts at: 830 2439 3470 830
Size: 1609 1031 3478
Fragments arranged by size:
3478 1609 1031
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2942 2942
Size: 6118
SacI G_AGCT'C
Cuts at: 2028 2028
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SexAI A'CCwGG_T
Cuts at: 2036 2036
Size: 6118
SfcI C'TryA_G
Cuts at: 826 1045 2435 3466 4601 5282 5473 826
Size: 219 1390 1031 1135 681 191 1471
Fragments arranged by size:
1471 1390 1135 1031 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3377 3445 1872
Size: 1505 68 4545
Fragments arranged by size:
4545 1505 68
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2160 3248 4222 5123 5385 820
Size: 197 926 217 1088 974 901 262 1553
Fragments arranged by size:
1553 1088 974 926 901 262 217 197
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2622 3093 3557 513
Size: 552 1557 471 464 3074
Fragments arranged by size:
3074 1557 552 471 464
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2045 2942 4145 4162 4315 4500 4945
Size: 1255 465 897 1203 17 153 185 445
Cuts at: 4945 325
Size: 1498
Fragments arranged by size:
1498 1255 1203 897 465 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3166 4364 6076
Size: 274 80 33 51 1443 1189 1198 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
1712 1443 1198 1189 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 2160 1943
Size: 217 5901
XmaI C'CCGG_G
Cuts at: 1870 3375 3443 1870
Size: 1505 68 4545
Fragments arranged by size:
4545 1505 68
XmnI GAAnn'nnTTC
Cuts at: 2283 2568 2580 4247 2283
Size: 285 12 1667 4154
Fragments arranged by size:
4154 1667 285 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoNI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI