Restriction map for
pFN21AE3201
(Circular) (Six-base) MAPSORT of: pFN21AE3201.seq Check: 2415 from: 1 to: 6118
pFN21AE3201 6118 bp
With 182 enzymes: SgfI *
March 28, 2012 12:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2535 3928 278
Size: 53 83 186 936 999 1393 2468
Fragments arranged by size:
2468 1393 999 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2538 4181 5565 5833 1632
Size: 906 1643 1384 268 1917
Fragments arranged by size:
1917 1643 1384 906 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 2715 5017 5994 1203
Size: 1512 2302 977 1327
Fragments arranged by size:
2302 1512 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 4847 4847
Size: 6118
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6118
AlwNI CAG_nnn'CTG
Cuts at: 2572 3861 5433 2572
Size: 1289 1572 3257
Fragments arranged by size:
3257 1572 1289
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2445 2805 3056 3427 3653 3805 5914 1493
Size: 952 360 251 371 226 152 2109 1697
Fragments arranged by size:
2109 1697 952 371 360 251 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 2370 3443 1870
Size: 73 427 1073 4545
Fragments arranged by size:
4545 1073 427 73
BamHI G'GATC_C
Cuts at: 3448 3815 3448
Size: 367 5751
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2029 3439 4894 618
Size: 325 217 627 242 1410 1455 1842
Fragments arranged by size:
1842 1455 1410 627 325 242 217
BanII G_rGCy'C
Cuts at: 1391 2331 3437 1391
Size: 940 1106 4072
Fragments arranged by size:
4072 1106 940
BbeI G_GCGC'C
Cuts at: 2033 2033
Size: 6118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2134 2173 2385 3309 928
Size: 971 235 39 212 924 3737
Fragments arranged by size:
3737 971 924 235 212 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2525 2559 4309 4343 1903
Size: 34 588 34 1750 34 3678
Fragments arranged by size:
3678 1750 588 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3119 4010 5220 3119
Size: 891 1210 4017
Fragments arranged by size:
4017 1210 891
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4729 136
Size: 107 122 71 631 605 337 2720 1525
Fragments arranged by size:
2720 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 3174 6113 3174
Size: 2939 3179
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 4179 5335 1790
Size: 2389 1156 2573
Fragments arranged by size:
2573 2389 1156
BmgBI CAC'GTC
Cuts at: 1318 3022 1318
Size: 1704 4414
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2856 3849 4807
Size: 416 53 83 399 24 1439 993 958
Cuts at: 4807 442
Size: 1753
Fragments arranged by size:
1753 1439 993 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3063 4778 1239
Size: 1824 1715 2579
Fragments arranged by size:
2579 1824 1715
Bpu10I CC'TnA_GC
Cuts at: 3012 3012
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2297 4243 5108 5406 2297
Size: 1946 865 298 3009
Fragments arranged by size:
3009 1946 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2012 2793 4781 882
Size: 617 513 781 1988 2219
Fragments arranged by size:
2219 1988 781 617 513
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2030 2532 3397 3925
Size: 53 83 186 936 497 502 865 528
Cuts at: 3925 4307 4942 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 865 635 528 502 497 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2318 2348 1178
Size: 30 165 30 915 30 4948
Fragments arranged by size:
4948 915 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2060 3296 3299 3302 3311 3371 3382 1186
Size: 874 1236 3 3 9 60 11 3922
Fragments arranged by size:
3922 1236 874 60 11 9 3 3
BseYI C'CCAG_C
Cuts at: 5321 5817 5321
Size: 496 5622
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5545
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2371 4179 4264 5335 2371
Size: 1808 85 1071 3154
Fragments arranged by size:
3154 1808 1071 85
BsmI GAATG_Cn'
Cuts at: 3629 3722 3629
Size: 93 6025
BsmBI CGTCTCn'nnnn_
Cuts at: 2018 3355 2018
Size: 1337 4781
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2371 3437 4179 4264 5335 1391
Size: 399 541 40 1066 742 85 1071 2174
Fragments arranged by size:
2174 1071 1066 742 541 399 85 40
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 2149 4007 5737 2149
Size: 1858 1730 2530
Fragments arranged by size:
2530 1858 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3473 844
Size: 2629 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2170 4613 4787 65
Size: 1183 922 2443 174 1396
Fragments arranged by size:
2443 1396 1183 922 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4762 4949 1700
Size: 110 2952 187 2869
Fragments arranged by size:
2952 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2899 96
Size: 2803 3315
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 2098 3174 3448 3815 4200 4217 5669 5755
Size: 126 1076 274 367 385 17 1452 86
Cuts at: 5755 5767 6113 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1076 385 367 346 274 126
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4618
Fragments arranged by size:
4618 939 552 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 2637 3634 4428 4448 2637
Size: 997 794 20 4307
Fragments arranged by size:
4307 997 794 20
ClaI AT'CG_AT
Cuts at: 1554 2431 1554
Size: 877 5241
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 3166 3422 3166
Size: 256 5862
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2044 5125 809
Size: 507 728 3081 1802
Fragments arranged by size:
3081 1802 728 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2010 3565 4454
Size: 54 1425 50 275 18 180 1555 889
Cuts at: 4454 6083 8
Size: 1629 43
Fragments arranged by size:
1629 1555 1425 889 275 180 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2196 2243 3261 3273 3288 3348 4048 1353
Size: 843 47 1018 12 15 60 700 3423
Fragments arranged by size:
3423 1018 843 700 60 47 15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2538 3063 4181 4778 5565 5833 1239
Size: 393 906 525 1118 597 787 268 1524
Fragments arranged by size:
1524 1118 906 787 597 525 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3038 3251 3038
Size: 213 5905
EcoRI G'AATT_C
Cuts at: 2445 3427 3805 2445
Size: 982 378 4758
Fragments arranged by size:
4758 982 378
EcoRV GAT'ATC
Cuts at: 2193 2193
Size: 6118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2975 2975
Size: 6118
FspI TGC'GCA
Cuts at: 4624 4624
Size: 6118
HaeII r_GCGC'y
Cuts at: 1678 2033 5265 1678
Size: 355 3232 2531
Fragments arranged by size:
3232 2531 355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2237 2269 4759 4791 4833
Size: 32 135 32 665 32 2490 32 42
Cuts at: 4833 4865 1373
Size: 32 2626
Fragments arranged by size:
2626 2490 665 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2414 748
Size: 1666 4452
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KasI G'GCGC_C
Cuts at: 2029 2029
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2207 2961 3239 5232 5416 1305
Size: 902 754 278 1993 184 2007
Fragments arranged by size:
2007 1993 902 754 278 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4296
Fragments arranged by size:
4296 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 4076 4435 4594 518
Size: 648 1464 1446 359 159 2042
Fragments arranged by size:
2042 1464 1446 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 2158 2543 2705 3402 3534 4211 5359
Size: 429 123 385 162 697 132 677 1148
Cuts at: 5359 5604 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 697 677 429 385 245 162
132 123
NarI GG'CG_CC
Cuts at: 2030 2030
Size: 6118
NcoI C'CATG_G
Cuts at: 513 1065 2004 2013 513
Size: 552 939 9 4618
Fragments arranged by size:
4618 939 552 9
NdeI CA'TA_TG
Cuts at: 387 3204 387
Size: 2817 3301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 2719 3213 3476 5021 1132
Size: 97 1490 494 263 1545 2229
Fragments arranged by size:
2229 1545 1490 494 263 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 2715 5017 2715
Size: 2302 3816
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3038 3251 3038
Size: 213 5905
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PstI C_TGCA'G
Cuts at: 830 2644 3470 830
Size: 1814 826 3478
Fragments arranged by size:
3478 1814 826
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 2543 2705 2543
Size: 162 5956
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SfcI C'TryA_G
Cuts at: 826 1045 2640 3231 3466 4601 5282 5473 826
Size: 219 1595 591 235 1135 681 191 1471
Fragments arranged by size:
1595 1471 1135 681 591 235 219 191
SfoI GGC'GCC
Cuts at: 2031 2031
Size: 6118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2312 2370 4222 5123 5385 820
Size: 197 926 369 58 1852 901 262 1553
Fragments arranged by size:
1852 1553 926 901 369 262 197 58
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 2321 4042 5
Size: 47 2269 1721 2081
Fragments arranged by size:
2269 2081 1721 47
StuI AGG'CCT
Cuts at: 1517 2300 2972 1517
Size: 783 672 4663
Fragments arranged by size:
4663 783 672
StyI C'CwwG_G
Cuts at: 513 1065 2004 2013 3557 513
Size: 552 939 9 1544 3074
Fragments arranged by size:
3074 1544 939 552 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2036 4145 4162 4315 4500 4945 325
Size: 1255 456 2109 17 153 185 445 1498
Fragments arranged by size:
2109 1498 1255 456 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2622 2899 4364
Size: 274 80 33 51 1443 645 277 1465
Cuts at: 4364 6076 96
Size: 1712 138
Fragments arranged by size:
1712 1465 1443 645 277 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2318 2350 6078 6110 2318
Size: 32 3728 32 2326
Fragments arranged by size:
3728 2326 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 2370 1943
Size: 427 5691
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 3030 4247 3030
Size: 1217 4901
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2533 3926 276
Size: 53 83 186 936 999 1393 2468
Fragments arranged by size:
2468 1393 999 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI