Restriction map for
pFN21AE3200
(Circular) (Six-base) MAPSORT of: pFN21AE3200.seq Check: 4421 from: 1 to: 11032
pFN21AE3200 11032 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5878 7169 7310 5878
Size: 1291 141 9600
Fragments arranged by size:
9600 1291 141
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2988 8842 278
Size: 53 83 186 936 1452 5854 2468
Fragments arranged by size:
5854 2468 1452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2295 4644 6783 8375 1937
Size: 358 2349 2139 1592 4594
Fragments arranged by size:
4594 2349 2139 1592 358
Acc65I G'GTAC_C
Cuts at: 4428 6201 8353 4428
Size: 1773 2152 7107
Fragments arranged by size:
7107 2152 1773
AclI AA'CG_TT
Cuts at: 1563 9159 9532 1563
Size: 7596 373 3063
Fragments arranged by size:
7596 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2537 3032 3429 3642 3812 4587 4632 4838
Size: 905 495 397 213 170 775 45 206
Cuts at: 4838 4895 5684 6275 7728 7884 9095 10479 10747
Size: 57 789 591 1453 156 1211 1384 268
Cuts at: 10747 1632
Size: 1917
Fragments arranged by size:
1917 1453 1384 1211 905 789 775 591
495 397 268 213 206 170 156 57
45
AfeI AGC'GCT
Cuts at: 2073 7665 8153 2073
Size: 5592 488 4952
Fragments arranged by size:
5592 4952 488
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10835
AflIII A'CryG_T
Cuts at: 1203 3693 4416 9931 10908 1203
Size: 2490 723 5515 977 1327
Fragments arranged by size:
5515 2490 1327 977 723
AgeI A'CCGG_T
Cuts at: 2223 2739 9863 2223
Size: 516 7124 3392
Fragments arranged by size:
7124 3392 516
AhdI GACnn_n'nnGTC
Cuts at: 9761 9761
Size: 11032
AleI CACnn'nnGTG
Cuts at: 1166 5861 7052 1166
Size: 4695 1191 5146
Fragments arranged by size:
5146 4695 1191
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 5750 7070 1184
Size: 4566 1320 5146
Fragments arranged by size:
5146 4566 1320
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3077 3109 3077
Size: 32 11000
AlwNI CAG_nnn'CTG
Cuts at: 3617 4870 4918 6190 6298 7384 8125 8775 10347
Size: 1253 48 1272 108 1086 741 650 1572
Cuts at: 10347 3617
Size: 4302
Fragments arranged by size:
4302 1572 1272 1253 1086 741 650 108
48
ApaI G_GGCC'C
Cuts at: 2155 4238 5873 6467 6484 7701 2155
Size: 2083 1635 594 17 1217 5486
Fragments arranged by size:
5486 2083 1635 1217 594 17
ApaLI G'TGCA_C
Cuts at: 4079 9089 10245 4079
Size: 5010 1156 4866
Fragments arranged by size:
5010 4866 1156
ApoI r'AATT_y
Cuts at: 1493 2253 2646 3017 3153 4799 6110 6154 7678
Size: 760 393 371 136 1646 1311 44 1524
Cuts at: 7678 8341 8567 8719 10828 1493
Size: 663 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 1524 1311 760 663 393
371 226 152 136 44
AscI GG'CGCG_CC
Cuts at: 7591 7591
Size: 11032
AseI AT'TA_AT
Cuts at: 160 1032 9586 160
Size: 872 8554 1606
Fragments arranged by size:
8554 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11032
AvaI C'yCGr_G
Cuts at: 1870 1943 2167 2193 7544 7575 8054 8357 1870
Size: 73 224 26 5351 31 479 303 4545
Fragments arranged by size:
5351 4545 479 303 224 73 31 26
BamHI G'GATC_C
Cuts at: 2506 6759 8322 8362 8729 2506
Size: 4253 1563 40 367 4809
Fragments arranged by size:
4809 4253 1563 367 40
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2997 4428 4443 5073 5348
Size: 325 217 627 1210 1431 15 630 275
Cuts at: 5348 6201 6441 7555 7580 7634 8353 9808 618
Size: 853 240 1114 25 54 719 1455 1842
Fragments arranged by size:
1842 1455 1431 1210 1114 853 719 630
627 325 275 240 217 54 25 15
BanII G_rGCy'C
Cuts at: 1391 2052 2155 2342 2583 2710 3360 4238 4714
Size: 661 103 187 241 127 650 878 476
Cuts at: 4714 5361 5565 5607 5873 5965 6467 6484 6660
Size: 647 204 42 266 92 502 17 176
Cuts at: 6660 7427 7701 7862 8063 8240 8259 8351 1391
Size: 767 274 161 201 177 19 92 4072
Fragments arranged by size:
4072 878 767 661 650 647 502 476
274 266 241 204 201 187 177 176
161 127 103 92 92 42 19 17
BbeI G_GCGC'C
Cuts at: 5077 6445 5077
Size: 1368 9664
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3264 3800 4221 7221 7479 7500 7515
Size: 971 1365 536 421 3000 258 21 15
Cuts at: 7515 7761 7791 7872 928
Size: 246 30 81 4088
Fragments arranged by size:
4088 3000 1365 971 536 421 258 246
81 30 21 15
BbvCI CC'TCA_GC
Cuts at: 2840 2840
Size: 11032
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5549 5583 5744 5778 9223 9257 1903
Size: 34 3612 34 161 34 3445 34 3678
Fragments arranged by size:
3678 3612 3445 161 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8924 10134 8924
Size: 1210 9822
BclI T'GATC_A
Cuts at: 1548 2451 3864 3924 1548
Size: 903 1413 60 8656
Fragments arranged by size:
8656 1413 903 60
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2468 4556 4858
Size: 107 122 71 631 605 796 2088 302
Cuts at: 4858 7063 7578 7601 9643 136
Size: 2205 515 23 2042 1525
Fragments arranged by size:
2205 2088 2042 1525 796 631 605 515
302 122 107 71 23
BglII A'GATC_T
Cuts at: 7236 11027 7236
Size: 3791 7241
BlpI GC'TnA_GC
Cuts at: 2801 7832 8449 2801
Size: 5031 617 5384
Fragments arranged by size:
5384 5031 617
Bme1580I G_kGCm'C
Cuts at: 1790 2155 4083 4197 4238 4446 5767 5873 6467
Size: 365 1928 114 41 208 1321 106 594
Cuts at: 6467 6484 7059 7583 7637 7701 8220 9093 10249
Size: 17 575 524 54 64 519 873 1156
Cuts at: 10249 1790
Size: 2573
Fragments arranged by size:
2573 1928 1321 1156 873 594 575 524
519 365 208 114 106 64 54 41
17
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11032
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3807 4373 4676
Size: 416 53 83 399 24 2390 566 303
Cuts at: 4676 8763 9721 442
Size: 4087 958 1753
Fragments arranged by size:
4087 2390 1753 958 566 416 399 303
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2912 1056
Size: 1856 9176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3079 4579 3079
Size: 1500 9532
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2036 2063 2108 2201 2567 2724 3063 4173
Size: 797 27 45 93 366 157 339 1110
Cuts at: 4173 4814 5285 5306 5355 5456 6167 6717 6860
Size: 641 471 21 49 101 711 550 143
Cuts at: 6860 7169 7211 7388 7406 7535 7652 7836 8102
Size: 309 42 177 18 129 117 184 266
Cuts at: 8102 8129 8147 8206 9692 1239
Size: 27 18 59 1486 2579
Fragments arranged by size:
2579 1486 1110 797 711 641 550 471
366 339 309 266 184 177 157 143
129 117 101 93 59 49 45 42
27 27 21 18 18
Bpu10I CC'TnA_GC
Cuts at: 2840 3320 3811 7067 2840
Size: 480 491 3256 6805
Fragments arranged by size:
6805 3256 491 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2987 5369 9157 10022 10320 2987
Size: 2382 3788 865 298 3699
Fragments arranged by size:
3788 3699 2382 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2583 4019 7613 9695 882
Size: 617 1084 1436 3594 2082 2219
Fragments arranged by size:
3594 2219 2082 1436 1084 617
BsaAI yAC'GTr
Cuts at: 493 1204 6540 8823 493
Size: 711 5336 2283 2702
Fragments arranged by size:
5336 2702 2283 711
BsaBI GATnn'nnATC
Cuts at: 4971 11026 4971
Size: 6055 4977
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2985 3380 4524 5074
Size: 53 83 186 936 1452 395 1144 550
Cuts at: 5074 6442 7476 7626 8839 9221 9856 275
Size: 1368 1034 150 1213 382 635 1451
Fragments arranged by size:
1452 1451 1368 1213 1144 1034 936 635
550 395 382 186 150 83 53
BsaWI w'CCGG_w
Cuts at: 2223 2739 3756 4140 5622 8145 9465 9863 10137
Size: 516 1017 384 1482 2523 1320 398 274
Cuts at: 10137 10284 10695 2223
Size: 147 411 2560
Fragments arranged by size:
2560 2523 1482 1320 1017 516 411 398
384 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2009 2039 3061 3091 4622
Size: 30 165 30 606 30 1022 30 1531
Cuts at: 4622 4652 5258 5288 1178
Size: 30 606 30 6922
Fragments arranged by size:
6922 1531 1022 606 606 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2717 2773 3068 3323 3407 3461 3464
Size: 1015 516 56 295 255 84 54 3
Cuts at: 3464 3469 3764 4528 4721 5228 6439 7074 7187
Size: 5 295 764 193 507 1211 635 113
Cuts at: 7187 7298 1186
Size: 111 4920
Fragments arranged by size:
4920 1211 1015 764 635 516 507 295
295 255 193 113 111 84 56 54
5 3
BseYI C'CCAG_C
Cuts at: 2621 2709 2830 2851 3528 4182 4707 5344 5574
Size: 88 121 21 677 654 525 637 230
Cuts at: 5574 6460 7599 7688 7744 8281 10235 10731 2621
Size: 886 1139 89 56 537 1954 496 2922
Fragments arranged by size:
2922 1954 1139 886 677 654 637 537
525 496 230 121 89 88 56 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2113 2615 6547 7030 7430 1163
Size: 573 377 502 3932 483 400 4765
Fragments arranged by size:
4765 3932 573 502 483 400 377
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4532 7957 7961 8482 9243 10271
Size: 275 186 2531 3425 4 521 761 1028
Cuts at: 10271 1540
Size: 2301
Fragments arranged by size:
3425 2531 2301 1028 761 521 275 186
4
BsiHKAI G_wGCw'C
Cuts at: 2583 2807 3360 4083 5565 5965 6311 8063 9093
Size: 224 553 723 1482 400 346 1752 1030
Cuts at: 9093 9178 10249 2583
Size: 85 1071 3366
Fragments arranged by size:
3366 1752 1482 1071 1030 723 553 400
346 224 85
BsmI GAATG_Cn'
Cuts at: 3213 5005 6597 8543 8636 3213
Size: 1792 1592 1946 93 5609
Fragments arranged by size:
5609 1946 1792 1592 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2084 2819 3685 2084
Size: 735 866 9431
Fragments arranged by size:
9431 866 735
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2052 2155 2342 2583 2710 2807 3360
Size: 399 262 103 187 241 127 97 553
Cuts at: 3360 4083 4197 4238 4446 4714 5361 5565 5607
Size: 723 114 41 208 268 647 204 42
Cuts at: 5607 5767 5873 5965 6311 6467 6484 6660 7059
Size: 160 106 92 346 156 17 176 399
Cuts at: 7059 7427 7583 7637 7701 7862 8063 8220 8240
Size: 368 156 54 64 161 201 157 20
Cuts at: 8240 8259 8351 9093 9178 10249 1391
Size: 19 92 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 723 647 553 399 399
368 346 268 262 241 208 204 201
187 176 161 160 157 156 156 127
114 106 103 97 92 92 85 64
54 42 41 20 19 17
BspEI T'CCGG_A
Cuts at: 3756 4140 8145 10695 3756
Size: 384 4005 2550 4093
Fragments arranged by size:
4093 4005 2550 384
BspHI T'CATG_A
Cuts at: 6054 8034 8921 10651 6054
Size: 1980 887 1730 6435
Fragments arranged by size:
6435 1980 1730 887
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4120 5133 5514 5878 6922 6948 7169 7310
Size: 3276 1013 381 364 1044 26 221 141
Cuts at: 7310 8387 844
Size: 1077 3489
Fragments arranged by size:
3489 3276 1077 1044 1013 381 364 221
141 26
BsrBI CCG'CTC
Cuts at: 1394 1823 3100 7082 7836 8919 1394
Size: 429 1277 3982 754 1083 3507
Fragments arranged by size:
3982 3507 1277 1083 754 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5835 5851 9527 9701 65
Size: 1183 4587 16 3676 174 1396
Fragments arranged by size:
4587 3676 1396 1183 174 16
BsrFI r'CCGG_y
Cuts at: 1700 1810 2082 2223 2559 2739 3324 5058 6438
Size: 110 272 141 336 180 585 1734 1380
Cuts at: 6438 6602 7956 9676 9863 1700
Size: 164 1354 1720 187 2869
Fragments arranged by size:
2869 1734 1720 1380 1354 585 336 272
187 180 164 141 110
BsrGI T'GTAC_A
Cuts at: 96 2411 4872 96
Size: 2315 2461 6256
Fragments arranged by size:
6256 2461 2315
BssHII G'CGCG_C
Cuts at: 1925 7406 7591 1925
Size: 5481 185 5366
Fragments arranged by size:
5481 5366 185
BssSI C'ACGA_G
Cuts at: 4654 8300 9092 10104 4654
Size: 3646 792 1012 5582
Fragments arranged by size:
5582 3646 1012 792
BstAPI GCAn_nnn'nTGC
Cuts at: 794 6561 7381 7453 794
Size: 5767 820 72 4373
Fragments arranged by size:
5767 4373 820 72
BstBI TT'CG_AA
Cuts at: 9836 9836
Size: 11032
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11032
BstXI CCAn_nnnn'nTGG
Cuts at: 2076 7513 2076
Size: 5437 5595
BstYI r'GATC_y
Cuts at: 1972 2506 2940 4069 4143 5397 6759 7236 8322
Size: 534 434 1129 74 1254 1362 477 1086
Cuts at: 8322 8362 8729 9114 9131 10583 10669 10681 11027
Size: 40 367 385 17 1452 86 12 346
Cuts at: 11027 1972
Size: 1977
Fragments arranged by size:
1977 1452 1362 1254 1129 1086 534 477
434 385 367 346 86 74 40 17
12
Bsu36I CC'TnA_GG
Cuts at: 6149 6149
Size: 11032
BtgI C'CryG_G
Cuts at: 513 1065 2004 4089 4461 4626 4950 5247 7257
Size: 552 939 2085 372 165 324 297 2010
Cuts at: 7257 7419 7961 8196 8241 513
Size: 162 542 235 45 3304
Fragments arranged by size:
3304 2085 2010 939 552 542 372 324
297 235 165 162 45
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10293
Fragments arranged by size:
10293 683 56
BtsI GCAGTG_nn'
Cuts at: 2601 5135 5439 7401 8548 9342 9362 2601
Size: 2534 304 1962 1147 794 20 4271
Fragments arranged by size:
4271 2534 1962 1147 794 304 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 11032
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 10997
DraI TTT'AAA
Cuts at: 3022 6106 8336 3022
Size: 3084 2230 5718
Fragments arranged by size:
5718 3084 2230
DraIII CAC_nnn'GTG
Cuts at: 3963 7453 3963
Size: 3490 7542
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3056 5482 10039 809
Size: 507 1740 2426 4557 1802
Fragments arranged by size:
4557 2426 1802 1740 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4529 6015 6711
Size: 54 1425 50 275 18 2699 1486 696
Cuts at: 6711 7837 7954 7958 7978 8479 9368 10997 8
Size: 1126 117 4 20 501 889 1629 43
Fragments arranged by size:
2699 1629 1486 1425 1126 889 696 501
275 117 54 50 43 20 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 4529 7954 7958 8479 1537
Size: 275 2717 3425 4 521 4090
Fragments arranged by size:
4090 3425 2717 521 275 4
EarI CTCTTCn'nnn_
Cuts at: 1353 2841 2931 3618 5574 5916 5953 6058 6416
Size: 1488 90 687 1956 342 37 105 358
Cuts at: 6416 6731 6852 7086 8189 8199 8962 1353
Size: 315 121 234 1103 10 763 3423
Fragments arranged by size:
3423 1956 1488 1103 763 687 358 342
315 234 121 105 90 37 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6353 9619 9993 10139 387
Size: 1064 138 4764 3266 374 146 1280
Fragments arranged by size:
4764 3266 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2581 3358 5563 5963 8061 2581
Size: 777 2205 400 2098 5552
Fragments arranged by size:
5552 2205 2098 777 400
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2036 2063 2108 2201 2537 2567 2724
Size: 393 404 27 45 93 336 30 157
Cuts at: 2724 3032 3063 3429 3642 3812 4173 4587 4632
Size: 308 31 366 213 170 361 414 45
Cuts at: 4632 4814 4838 4895 5285 5306 5355 5456 5684
Size: 182 24 57 390 21 49 101 228
Cuts at: 5684 6167 6275 6717 6860 7169 7211 7388 7406
Size: 483 108 442 143 309 42 177 18
Cuts at: 7406 7535 7652 7728 7836 7884 8102 8129 8147
Size: 129 117 76 108 48 218 27 18
Cuts at: 8147 8206 9095 9692 10479 10747 1239
Size: 59 889 597 787 268 1524
Fragments arranged by size:
1524 889 787 597 483 442 414 404
393 390 366 361 336 309 308 268
228 218 213 182 177 170 157 143
129 117 108 108 101 93 76 59
57 49 48 45 45 42 31 30
27 27 24 21 18 18
EcoNI CCTnn'n_nnAGG
Cuts at: 2431 4004 5068 5228 6017 6526 2431
Size: 1573 1064 160 789 509 6937
Fragments arranged by size:
6937 1573 1064 789 509 160
EcoO109I rG'GnC_Cy
Cuts at: 2152 2272 2484 3483 4234 5671 5869 6464 6481
Size: 120 212 999 751 1437 198 595 17
Cuts at: 6481 7360 7729 8086 8122 2152
Size: 879 369 357 36 5062
Fragments arranged by size:
5062 1437 999 879 751 595 369 357
212 198 120 36 17
EcoRI G'AATT_C
Cuts at: 6154 8341 8719 6154
Size: 2187 378 8467
Fragments arranged by size:
8467 2187 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4492 4637 6445 4492
Size: 145 1808 9079
Fragments arranged by size:
9079 1808 145
FseI GG_CCGG'CC
Cuts at: 7960 7960
Size: 11032
FspI TGC'GCA
Cuts at: 6918 7292 9538 6918
Size: 374 2246 8412
Fragments arranged by size:
8412 2246 374
HaeII r_GCGC'y
Cuts at: 1678 2075 5077 5247 5494 6445 6895 7667 8155
Size: 397 3002 170 247 951 450 772 488
Cuts at: 8155 10179 1678
Size: 2024 2531
Fragments arranged by size:
3002 2531 2024 951 772 488 450 397
247 170
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3061 3093 3517 3549 3916
Size: 32 135 32 1489 32 424 32 367
Cuts at: 3916 3948 4637 4669 5924 5956 6614 6646 9673
Size: 32 689 32 1255 32 658 32 3027
Cuts at: 9673 9705 9747 9779 1373
Size: 32 42 32 2626
Fragments arranged by size:
3027 2626 1489 1255 689 658 424 367
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4645 5014 6436 6451 7015 8308
Size: 988 281 2707 369 1422 15 564 1293
Cuts at: 8308 8376 8622 669
Size: 68 246 3079
Fragments arranged by size:
3079 2707 1422 1293 988 564 369 281
246 68 15
HindIII A'AGCT_T
Cuts at: 748 2786 4474 6122 6817 748
Size: 2038 1688 1648 695 4963
Fragments arranged by size:
4963 2038 1688 1648 695
HpaI GTT'AAC
Cuts at: 8622 8622
Size: 11032
KasI G'GCGC_C
Cuts at: 5073 6441 5073
Size: 1368 9664
KpnI G_GTAC'C
Cuts at: 4432 6205 8357 4432
Size: 1773 2152 7107
Fragments arranged by size:
7107 2152 1773
MfeI C'AATT_G
Cuts at: 6216 8631 6216
Size: 2415 8617
MluI A'CGCG_T
Cuts at: 10908 10908
Size: 11032
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2007 3417 5932 6025 6591 10146 10330 1305
Size: 702 1410 2515 93 566 3555 184 2007
Fragments arranged by size:
3555 2515 2007 1410 702 566 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 7980 10
Size: 54 1425 343 6148 3062
Fragments arranged by size:
6148 3062 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2306 2777 3039 3740 5861 6746 6905
Size: 648 1140 471 262 701 2121 885 159
Cuts at: 6905 7052 7640 8990 9349 9508 518
Size: 147 588 1350 359 159 2042
Fragments arranged by size:
2121 2042 1350 1140 885 701 648 588
471 359 262 159 159 147
MspA1I CmG'CkG
Cuts at: 1606 2113 4261 4816 5773 7044 7076 7189 7599
Size: 507 2148 555 957 1271 32 113 410
Cuts at: 7599 7763 7769 7831 8448 9125 10273 10518 1606
Size: 164 6 62 617 677 1148 245 2120
Fragments arranged by size:
2148 2120 1271 1148 957 677 617 555
507 410 245 164 113 62 32 6
NaeI GCC'GGC
Cuts at: 2084 3326 5060 6604 7958 2084
Size: 1242 1734 1544 1354 5158
Fragments arranged by size:
5158 1734 1544 1354 1242
NarI GG'CG_CC
Cuts at: 5074 6442 5074
Size: 1368 9664
NcoI C'CATG_G
Cuts at: 513 1065 2004 4089 5247 7419 8241 513
Size: 552 939 2085 1158 2172 822 3304
Fragments arranged by size:
3304 2172 2085 1158 939 822 552
NdeI CA'TA_TG
Cuts at: 387 2307 387
Size: 1920 9112
NgoMIV G'CCGG_C
Cuts at: 2082 3324 5058 6602 7956 2082
Size: 1242 1734 1544 1354 5158
Fragments arranged by size:
5158 1734 1544 1354 1242
NheI G'CTAG_C
Cuts at: 1052 2908 1052
Size: 1856 9176
NotI GC'GGCC_GC
Cuts at: 8479 8479
Size: 11032
NspI r_CATG'y
Cuts at: 1132 1229 4420 5912 8390 9935 1132
Size: 97 3191 1492 2478 1545 2229
Fragments arranged by size:
3191 2478 2229 1545 1492 97
PasI CC'CwG_GG
Cuts at: 1348 5675 1348
Size: 4327 6705
PciI A'CATG_T
Cuts at: 4416 9931 4416
Size: 5515 5517
PflMI CCAn_nnn'nTGG
Cuts at: 1108 6283 6460 6583 8125 1108
Size: 5175 177 123 1542 4015
Fragments arranged by size:
5175 4015 1542 177 123
PfoI T'CCnGG_A
Cuts at: 2217 2544 4357 6183 6762 7365 7668 2217
Size: 327 1813 1826 579 603 303 5581
Fragments arranged by size:
5581 1826 1813 603 579 327 303
PmeI GTTT'AAAC
Cuts at: 8336 8336
Size: 11032
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3769 3801 9101 9133 10614 10646 3769
Size: 32 5300 32 1481 32 4155
Fragments arranged by size:
5300 4155 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2272 3483 5671 8086 2272
Size: 1211 2188 2415 5218
Fragments arranged by size:
5218 2415 2188 1211
PshAI GACnn'nnGTC
Cuts at: 1613 2984 3264 4037 1613
Size: 1371 280 773 8608
Fragments arranged by size:
8608 1371 773 280
PsiI TTA'TAA
Cuts at: 8602 8602
Size: 11032
PspOMI G'GGCC_C
Cuts at: 2151 4234 5869 6463 6480 7697 2151
Size: 2083 1635 594 17 1217 5486
Fragments arranged by size:
5486 2083 1635 1217 594 17
PstI C_TGCA'G
Cuts at: 830 5130 5229 5772 7166 7768 8094 8384 830
Size: 4300 99 543 1394 602 326 290 3478
Fragments arranged by size:
4300 3478 1394 602 543 326 290 99
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11032
PvuII CAG'CTG
Cuts at: 4261 7189 7599 7831 4261
Size: 2928 410 232 7462
Fragments arranged by size:
7462 2928 410 232
SacI G_AGCT'C
Cuts at: 2583 3360 5565 5965 8063 2583
Size: 777 2205 400 2098 5552
Fragments arranged by size:
5552 2205 2098 777 400
SalI G'TCGA_C
Cuts at: 1936 4643 8374 1936
Size: 2707 3731 4594
Fragments arranged by size:
4594 3731 2707
SanDI GG'GwC_CC
Cuts at: 2272 3483 2272
Size: 1211 9821
SapI GCTCTTCn'nnn_
Cuts at: 2841 5574 5953 7086 8189 2841
Size: 2733 379 1133 1103 5684
Fragments arranged by size:
5684 2733 1133 1103 379
SbfI CC_TGCA'GG
Cuts at: 8384 8384
Size: 11032
ScaI AGT'ACT
Cuts at: 9280 10992 9280
Size: 1712 9320
SexAI A'CCwGG_T
Cuts at: 5795 5795
Size: 11032
SfcI C'TryA_G
Cuts at: 826 1045 2210 4937 5126 5225 5768 6526 7162
Size: 219 1165 2727 189 99 543 758 636
Cuts at: 7162 7764 8090 8380 9515 10196 10387 826
Size: 602 326 290 1135 681 191 1471
Fragments arranged by size:
2727 1471 1165 1135 758 681 636 602
543 326 290 219 191 189 99
SfoI GGC'GCC
Cuts at: 5075 6443 5075
Size: 1368 9664
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11032
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11032
SmaI CCC'GGG
Cuts at: 1872 2169 7577 8359 1872
Size: 297 5408 782 4545
Fragments arranged by size:
5408 4545 782 297
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3002 5384 9136 10037 10299 820
Size: 197 926 1059 2382 3752 901 262 1553
Fragments arranged by size:
3752 2382 1553 1059 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 8823 493
Size: 8330 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11032
SphI G_CATG'C
Cuts at: 1132 5912 8390 1132
Size: 4780 2478 3774
Fragments arranged by size:
4780 3774 2478
SspI AAT'ATT
Cuts at: 5 52 5533 8956 5
Size: 47 5481 3423 2081
Fragments arranged by size:
5481 3423 2081 47
StuI AGG'CCT
Cuts at: 1517 4559 5990 6404 8044 8173 1517
Size: 3042 1431 414 1640 129 4376
Fragments arranged by size:
4376 3042 1640 1431 414 129
StyI C'CwwG_G
Cuts at: 513 1065 2004 2913 3552 4089 4198 5247 5859
Size: 552 939 909 639 537 109 1049 612
Cuts at: 5859 7230 7308 7419 7436 7693 8168 8241 8262
Size: 1371 78 111 17 257 475 73 21
Cuts at: 8262 8471 513
Size: 209 3074
Fragments arranged by size:
3074 1371 1049 939 909 639 612 552
537 475 257 209 111 109 78 73
21 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2782 2996 3549 3597 5336 5613 5631
Size: 1255 1202 214 553 48 1739 277 18
Cuts at: 5631 7637 7652 9059 9076 9229 9414 9859 325
Size: 2006 15 1407 17 153 185 445 1498
Fragments arranged by size:
2006 1739 1498 1407 1255 1202 553 445
277 214 185 153 48 18 17 15
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2411 3285 4872
Size: 274 80 33 51 1443 434 874 1587
Cuts at: 4872 5289 6378 9278 10990 96
Size: 417 1089 2900 1712 138
Fragments arranged by size:
2900 1712 1587 1443 1089 874 434 417
274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5258 5290 5448 5480 6693 6725 10992 11024 5258
Size: 32 158 32 1213 32 4267 32 5266
Fragments arranged by size:
5266 4267 1213 158 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2033 1316
Size: 717 10315
XbaI T'CTAG_A
Cuts at: 4274 8368 4274
Size: 4094 6938
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2951 3373 3985 4459 5880 6667 2011
Size: 940 422 612 474 1421 787 6376
Fragments arranged by size:
6376 1421 940 787 612 474 422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11032
XmaI C'CCGG_G
Cuts at: 1870 2167 7575 8357 1870
Size: 297 5408 782 4545
Fragments arranged by size:
5408 4545 782 297
XmnI GAAnn'nnTTC
Cuts at: 4959 6404 9161 4959
Size: 1445 2757 6830
Fragments arranged by size:
6830 2757 1445
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2986 8840 276
Size: 53 83 186 936 1452 5854 2468
Fragments arranged by size:
5854 2468 1452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FseI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI
SrfI SwaI