(Circular) (Six-base) MAPSORT of: pFN21AE3198.seq Check: 2891 from: 1 to: 5200 pFN21AE3198 5200 bp With 182 enzymes: SgfI * December 8, 2014 13:39 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2433 3010 278 Size: 53 83 186 936 897 577 2468 Fragments arranged by size: 2468 936 897 577 186 83 53 AccI GT'mk_AC Cuts at: 1937 2543 1937 Size: 606 4594 Acc65I G'GTAC_C Cuts at: 2521 2521 Size: 5200 AclI AA'CG_TT Cuts at: 1563 3327 3700 1563 Size: 1764 373 3063 Fragments arranged by size: 3063 1764 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2383 3263 4647 4915 1632 Size: 751 880 1384 268 1917 Fragments arranged by size: 1917 1384 880 751 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5003 AflIII A'CryG_T Cuts at: 1203 4099 5076 1203 Size: 2896 977 1327 Fragments arranged by size: 2896 1327 977 AgeI A'CCGG_T Cuts at: 4031 4031 Size: 5200 AhdI GACnn_n'nnGTC Cuts at: 3929 3929 Size: 5200 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5200 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5200 AlwNI CAG_nnn'CTG Cuts at: 2296 2372 2943 4515 2296 Size: 76 571 1572 2981 Fragments arranged by size: 2981 1572 571 76 ApaI G_GGCC'C Cuts at: 2220 2220 Size: 5200 ApaLI G'TGCA_C Cuts at: 3257 4413 3257 Size: 1156 4044 ApoI r'AATT_y Cuts at: 1493 2509 2735 2887 4996 1493 Size: 1016 226 152 2109 1697 Fragments arranged by size: 2109 1697 1016 226 152 AseI AT'TA_AT Cuts at: 160 1032 3754 160 Size: 872 2722 1606 Fragments arranged by size: 2722 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5200 AvaI C'yCGr_G Cuts at: 1870 1943 2525 1870 Size: 73 582 4545 Fragments arranged by size: 4545 582 73 BamHI G'GATC_C Cuts at: 2331 2530 2897 2331 Size: 199 367 4634 Fragments arranged by size: 4634 367 199 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2013 2190 2521 3976 618 Size: 325 217 627 226 177 331 1455 1842 Fragments arranged by size: 1842 1455 627 331 325 226 217 177 BanII G_rGCy'C Cuts at: 1391 2035 2220 2321 2357 2519 1391 Size: 644 185 101 36 162 4072 Fragments arranged by size: 4072 644 185 162 101 36 BbeI G_GCGC'C Cuts at: 2017 2194 2017 Size: 177 5023 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4229 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3391 3425 1903 Size: 34 1454 34 3678 Fragments arranged by size: 3678 1454 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3092 4302 3092 Size: 1210 3990 BclI T'GATC_A Cuts at: 1548 1548 Size: 5200 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2261 3811 136 Size: 107 122 71 631 605 589 1550 1525 Fragments arranged by size: 1550 1525 631 605 589 122 107 71 BglII A'GATC_T Cuts at: 5195 5195 Size: 5200 BlpI GC'TnA_GC Cuts at: 2617 2617 Size: 5200 Bme1580I G_kGCm'C Cuts at: 1790 2220 3261 4417 1790 Size: 430 1041 1156 2573 Fragments arranged by size: 2573 1156 1041 430 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5200 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2931 3889 442 Size: 416 53 83 399 24 1514 958 1753 Fragments arranged by size: 1753 1514 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3860 1239 Size: 2621 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3325 4190 4488 3325 Size: 865 298 4037 Fragments arranged by size: 4037 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3863 882 Size: 617 2364 2219 Fragments arranged by size: 2364 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2113 2991 493 Size: 711 909 878 2702 Fragments arranged by size: 2702 909 878 711 BsaBI GATnn'nnATC Cuts at: 5194 5194 Size: 5200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2014 2191 2430 3007 Size: 53 83 186 936 481 177 239 577 Cuts at: 3007 3389 4024 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 577 481 382 239 186 177 83 53 BsaWI w'CCGG_w Cuts at: 2383 3633 4031 4305 4452 4863 2383 Size: 1250 398 274 147 411 2720 Fragments arranged by size: 2720 1250 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 1178 Size: 30 165 30 819 30 4126 Fragments arranged by size: 4126 819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2256 1186 Size: 1070 4130 BseYI C'CCAG_C Cuts at: 2440 4403 4899 2440 Size: 1963 496 2741 Fragments arranged by size: 2741 1963 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4627 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2048 2211 2650 3411 4439 1540 Size: 275 186 47 163 439 761 1028 2301 Fragments arranged by size: 2301 1028 761 439 275 186 163 47 BsiHKAI G_wGCw'C Cuts at: 2321 3261 3346 4417 2321 Size: 940 85 1071 3104 Fragments arranged by size: 3104 1071 940 85 BsmI GAATG_Cn' Cuts at: 2711 2804 2711 Size: 93 5107 BsmBI CGTCTCn'nnnn_ Cuts at: 2297 2421 2437 2297 Size: 124 16 5060 Fragments arranged by size: 5060 124 16 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2035 2220 2321 2357 2519 3261 3346 Size: 399 245 185 101 36 162 742 85 Cuts at: 3346 4417 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 245 185 162 101 85 36 BspEI T'CCGG_A Cuts at: 4863 4863 Size: 5200 BspHI T'CATG_A Cuts at: 3089 4819 3089 Size: 1730 3470 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2555 844 Size: 1711 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2026 3087 1394 Size: 429 203 1061 3507 Fragments arranged by size: 3507 1061 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 3695 3869 65 Size: 1183 2447 174 1396 Fragments arranged by size: 2447 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2043 3844 4031 1700 Size: 110 233 1801 187 2869 Fragments arranged by size: 2869 1801 233 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5200 BssHII G'CGCG_C Cuts at: 1925 2196 2203 2359 1925 Size: 271 7 156 4766 Fragments arranged by size: 4766 271 156 7 BssSI C'ACGA_G Cuts at: 2281 3260 4272 2281 Size: 979 1012 3209 Fragments arranged by size: 3209 1012 979 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5200 BstBI TT'CG_AA Cuts at: 4004 4004 Size: 5200 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5200 BstYI r'GATC_y Cuts at: 1972 2331 2530 2897 3282 3299 4751 4837 4849 Size: 359 199 367 385 17 1452 86 12 Cuts at: 4849 5195 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 359 346 199 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2048 513 Size: 552 939 44 3665 Fragments arranged by size: 3665 939 552 44 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4461 Fragments arranged by size: 4461 683 56 BtsI GCAGTG_nn' Cuts at: 2057 2716 3510 3530 2057 Size: 659 794 20 3727 Fragments arranged by size: 3727 794 659 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5200 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5165 DraI TTT'AAA Cuts at: 2504 2504 Size: 5200 DraIII CAC_nnn'GTG Cuts at: 2137 2137 Size: 5200 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4207 809 Size: 507 2891 1802 Fragments arranged by size: 2891 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2045 2208 2223 Size: 54 1425 50 275 18 215 163 15 Cuts at: 2223 2410 2647 3536 5165 8 Size: 187 237 889 1629 43 Fragments arranged by size: 1629 1425 889 275 237 215 187 163 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 2045 2208 2647 1537 Size: 275 233 163 439 4090 Fragments arranged by size: 4090 439 275 233 163 EarI CTCTTCn'nnn_ Cuts at: 1353 3130 1353 Size: 1777 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3787 4161 4307 387 Size: 1064 138 2198 374 146 1280 Fragments arranged by size: 2198 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2319 2319 Size: 5200 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2383 3263 3860 4647 4915 1239 Size: 393 751 880 597 787 268 1524 Fragments arranged by size: 1524 880 787 751 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2217 2217 Size: 5200 EcoRI G'AATT_C Cuts at: 2509 2887 2509 Size: 378 4822 FspI TGC'GCA Cuts at: 3706 3706 Size: 5200 HaeII r_GCGC'y Cuts at: 1678 2017 2194 4347 1678 Size: 339 177 2153 2531 Fragments arranged by size: 2531 2153 339 177 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 3841 3873 3915 Size: 32 135 32 650 32 1587 32 42 Cuts at: 3915 3947 1373 Size: 32 2626 Fragments arranged by size: 2626 1587 650 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2544 2790 669 Size: 988 281 606 246 3079 Fragments arranged by size: 3079 988 606 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5200 HpaI GTT'AAC Cuts at: 2790 2790 Size: 5200 KasI G'GCGC_C Cuts at: 2013 2190 2013 Size: 177 5023 KpnI G_GTAC'C Cuts at: 2525 2525 Size: 5200 MfeI C'AATT_G Cuts at: 2799 2799 Size: 5200 MluI A'CGCG_T Cuts at: 5076 5076 Size: 5200 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4314 4498 1305 Size: 3009 184 2007 Fragments arranged by size: 3009 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2225 2412 10 Size: 54 1425 343 393 187 2798 Fragments arranged by size: 2798 1425 393 343 187 54 MslI CAynn'nnrTG Cuts at: 518 1166 3158 3517 3676 518 Size: 648 1992 359 159 2042 Fragments arranged by size: 2042 1992 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2086 2150 2403 2444 2616 3293 4441 Size: 444 36 64 253 41 172 677 1148 Cuts at: 4441 4686 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 444 253 245 172 64 41 36 NaeI GCC'GGC Cuts at: 2045 2045 Size: 5200 NarI GG'CG_CC Cuts at: 2014 2191 2014 Size: 177 5023 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3709 Fragments arranged by size: 3709 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5200 NgoMIV G'CCGG_C Cuts at: 2043 2043 Size: 5200 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5200 NotI GC'GGCC_GC Cuts at: 2208 2647 2208 Size: 439 4761 NspI r_CATG'y Cuts at: 1132 1229 2558 4103 1132 Size: 97 1329 1545 2229 Fragments arranged by size: 2229 1545 1329 97 PasI CC'CwG_GG Cuts at: 1348 2018 1348 Size: 670 4530 PciI A'CATG_T Cuts at: 4099 4099 Size: 5200 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2137 1108 Size: 902 127 4171 Fragments arranged by size: 4171 902 127 PmeI GTTT'AAAC Cuts at: 2504 2504 Size: 5200 PmlI CAC'GTG Cuts at: 2113 2113 Size: 5200 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3269 3301 4782 4814 3269 Size: 32 1481 32 3655 Fragments arranged by size: 3655 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5200 PsiI TTA'TAA Cuts at: 2770 2770 Size: 5200 PspOMI G'GGCC_C Cuts at: 2216 2216 Size: 5200 PstI C_TGCA'G Cuts at: 830 2552 830 Size: 1722 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5200 RsrII CG'GwC_CG Cuts at: 2492 2492 Size: 5200 SacI G_AGCT'C Cuts at: 2321 2321 Size: 5200 SacII CC_GC'GG Cuts at: 2051 2051 Size: 5200 SalI G'TCGA_C Cuts at: 1936 2542 1936 Size: 606 4594 SbfI CC_TGCA'GG Cuts at: 2552 2552 Size: 5200 ScaI AGT'ACT Cuts at: 3448 5160 3448 Size: 1712 3488 SfcI C'TryA_G Cuts at: 826 1045 2548 3683 4364 4555 826 Size: 219 1503 1135 681 191 1471 Fragments arranged by size: 1503 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2015 2192 2015 Size: 177 5023 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5200 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5200 SmaI CCC'GGG Cuts at: 1872 2527 1872 Size: 655 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3304 4205 4467 820 Size: 197 926 1361 901 262 1553 Fragments arranged by size: 1553 1361 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2991 493 Size: 2498 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5200 SphI G_CATG'C Cuts at: 1132 2558 1132 Size: 1426 3774 SspI AAT'ATT Cuts at: 5 52 3124 5 Size: 47 3072 2081 Fragments arranged by size: 3072 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5200 StyI C'CwwG_G Cuts at: 513 1065 2004 2467 2639 513 Size: 552 939 463 172 3074 Fragments arranged by size: 3074 939 552 463 172 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3227 3244 3397 3582 4027 325 Size: 1255 1647 17 153 185 445 1498 Fragments arranged by size: 1647 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3446 5158 96 Size: 274 80 33 51 1443 1469 1712 138 Fragments arranged by size: 1712 1469 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5160 5192 5160 Size: 32 5168 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5200 XbaI T'CTAG_A Cuts at: 2536 2536 Size: 5200 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5200 XmaI C'CCGG_G Cuts at: 1870 2525 1870 Size: 655 4545 XmnI GAAnn'nnTTC Cuts at: 3329 3329 Size: 5200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2431 3008 276 Size: 53 83 186 936 897 577 2468 Fragments arranged by size: 2468 936 897 577 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII SanDI SapI SexAI SfiI SrfI SwaI XcmI