Restriction map for
pFN21AE3196
(Circular) (Six-base) MAPSORT of: pFN21AE3196.seq Check: 5905 from: 1 to: 6115
pFN21AE3196 6115 bp
With 182 enzymes: SgfI *
October 25, 2019 12:32 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2938 2938
Size: 6115
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3925 278
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3458 1937
Size: 1521 4594
Acc65I G'GTAC_C
Cuts at: 3436 3436
Size: 6115
AclI AA'CG_TT
Cuts at: 1563 4242 4615 1563
Size: 2679 373 3063
Fragments arranged by size:
3063 2679 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2204 4178 5562 5830 1632
Size: 572 1974 1384 268 1917
Fragments arranged by size:
1974 1917 1384 572 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5918
AflIII A'CryG_T
Cuts at: 1203 5014 5991 1203
Size: 3811 977 1327
Fragments arranged by size:
3811 1327 977
AgeI A'CCGG_T
Cuts at: 4946 4946
Size: 6115
AhdI GACnn_n'nnGTC
Cuts at: 4844 4844
Size: 6115
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2369 2699 2870 1184
Size: 1185 330 171 4429
Fragments arranged by size:
4429 1185 330 171
AlwNI CAG_nnn'CTG
Cuts at: 2597 3858 5430 2597
Size: 1261 1572 3282
Fragments arranged by size:
3282 1572 1261
ApaI G_GGCC'C
Cuts at: 2960 2960
Size: 6115
ApaLI G'TGCA_C
Cuts at: 3323 4172 5328 3323
Size: 849 1156 4110
Fragments arranged by size:
4110 1156 849
ApoI r'AATT_y
Cuts at: 1493 3424 3650 3802 5911 1493
Size: 1931 226 152 2109 1697
Fragments arranged by size:
2109 1931 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4669 160
Size: 872 3637 1606
Fragments arranged by size:
3637 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 2283 3440 1870
Size: 73 119 221 1157 4545
Fragments arranged by size:
4545 1157 221 119 73
BamHI G'GATC_C
Cuts at: 2266 3445 3812 2266
Size: 1179 367 4569
Fragments arranged by size:
4569 1179 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2846 3016 3436 4891 618
Size: 325 217 627 1059 170 420 1455 1842
Fragments arranged by size:
1842 1455 1059 627 420 325 217 170
BanII G_rGCy'C
Cuts at: 1391 2136 2535 2960 3255 3366 3411 3434 1391
Size: 745 399 425 295 111 45 23 4072
Fragments arranged by size:
4072 745 425 399 295 111 45 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2664 928
Size: 971 765 4379
Fragments arranged by size:
4379 971 765
BbvCI CC'TCA_GC
Cuts at: 2391 2391
Size: 6115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4306 4340 1903
Size: 34 2369 34 3678
Fragments arranged by size:
3678 2369 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4007 5217 4007
Size: 1210 4905
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6115
BfrBI ATG'CAT
Cuts at: 3118 3118
Size: 6115
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2286 4726 136
Size: 107 122 71 631 605 614 2440 1525
Fragments arranged by size:
2440 1525 631 614 605 122 107 71
BglII A'GATC_T
Cuts at: 6110 6110
Size: 6115
BlpI GC'TnA_GC
Cuts at: 2762 3532 2762
Size: 770 5345
Bme1580I G_kGCm'C
Cuts at: 1790 2849 2960 3000 3021 3231 3327 4176 5332
Size: 1059 111 40 21 210 96 849 1156
Cuts at: 5332 1790
Size: 2573
Fragments arranged by size:
2573 1156 1059 849 210 111 96 40
21
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6115
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2560 3846 4804
Size: 416 53 83 399 24 1143 1286 958
Cuts at: 4804 442
Size: 1753
Fragments arranged by size:
1753 1286 1143 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2520 3005 4775 1239
Size: 1281 485 1770 2579
Fragments arranged by size:
2579 1770 1281 485
Bpu10I CC'TnA_GC
Cuts at: 2391 2391
Size: 6115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1998 4240 5105 5403 1998
Size: 2242 865 298 2710
Fragments arranged by size:
2710 2242 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2549 4778 882
Size: 617 1050 2229 2219
Fragments arranged by size:
2229 2219 1050 617
BsaAI yAC'GTr
Cuts at: 493 1204 2374 3130 3906 493
Size: 711 1170 756 776 2702
Fragments arranged by size:
2702 1170 776 756 711
BsaBI GATnn'nnATC
Cuts at: 6109 6109
Size: 6115
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2154 2818 3111
Size: 53 83 186 936 558 63 664 293
Cuts at: 3111 3922 4304 4939 275
Size: 811 382 635 1451
Fragments arranged by size:
1451 936 811 664 635 558 382 293
186 83 63 53
BsaWI w'CCGG_w
Cuts at: 2364 4548 4946 5220 5367 5778 2364
Size: 2184 398 274 147 411 2701
Fragments arranged by size:
2701 2184 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5890
Fragments arranged by size:
5890 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 3002 1186
Size: 1291 525 4299
Fragments arranged by size:
4299 1291 525
BseYI C'CCAG_C
Cuts at: 2467 2475 2587 3371 3384 5318 5814 2467
Size: 8 112 784 13 1934 496 2768
Fragments arranged by size:
2768 1934 784 496 112 13 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2396 2659 2711 3038 3069 3123 3332
Size: 573 660 263 52 327 31 54 209
Cuts at: 3332 1163
Size: 3946
Fragments arranged by size:
3946 660 573 327 263 209 54 52
31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2459 2887 3565 4326 5354 1540
Size: 275 186 458 428 678 761 1028 2301
Fragments arranged by size:
2301 1028 761 678 458 428 275 186
BsiHKAI G_wGCw'C
Cuts at: 2101 2295 2763 3327 4176 4261 5332 2101
Size: 194 468 564 849 85 1071 2884
Fragments arranged by size:
2884 1071 849 564 468 194 85
BsmI GAATG_Cn'
Cuts at: 3212 3626 3719 3212
Size: 414 93 5608
Fragments arranged by size:
5608 414 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2496 2496
Size: 6115
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2136 2295 2535 2763 2849 2960
Size: 399 311 35 159 240 228 86 111
Cuts at: 2960 3000 3021 3231 3255 3327 3366 3411 3434
Size: 40 21 210 24 72 39 45 23
Cuts at: 3434 4176 4261 5332 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 311 240 228 210
159 111 86 85 72 45 40 39
35 24 23 21
BspEI T'CCGG_A
Cuts at: 2364 5778 2364
Size: 3414 2701
BspHI T'CATG_A
Cuts at: 4004 5734 4004
Size: 1730 4385
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2635 2724 2938 3470 844
Size: 1791 89 214 532 3489
Fragments arranged by size:
3489 1791 532 214 89
BsrBI CCG'CTC
Cuts at: 1394 1823 2012 2428 2542 4002 1394
Size: 429 189 416 114 1460 3507
Fragments arranged by size:
3507 1460 429 416 189 114
BsrDI GCAATG_nn'
Cuts at: 65 1248 2781 4610 4784 65
Size: 1183 1533 1829 174 1396
Fragments arranged by size:
1829 1533 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2093 2142 2694 4759 4946 1700
Size: 110 283 49 552 2065 187 2869
Fragments arranged by size:
2869 2065 552 283 187 110 49
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6115
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6115
BssSI C'ACGA_G
Cuts at: 2516 3147 4175 5187 2516
Size: 631 1028 1012 3444
Fragments arranged by size:
3444 1028 1012 631
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6115
BstBI TT'CG_AA
Cuts at: 4919 4919
Size: 6115
BstEII G'GTnAC_C
Cuts at: 1184 2647 1184
Size: 1463 4652
BstXI CCAn_nnnn'nTGG
Cuts at: 2633 2633
Size: 6115
BstYI r'GATC_y
Cuts at: 1972 2266 3445 3812 4197 4214 5666 5752 5764
Size: 294 1179 367 385 17 1452 86 12
Cuts at: 5764 6110 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1179 385 367 346 294 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2109 2202 2249 2297 2881 3090 3177
Size: 552 1044 93 47 48 584 209 87
Cuts at: 3177 3231 513
Size: 54 3397
Fragments arranged by size:
3397 1044 584 552 209 93 87 54
48 47
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5376
Fragments arranged by size:
5376 683 56
BtsI GCAGTG_nn'
Cuts at: 3047 3076 3285 3631 4425 4445 3047
Size: 29 209 346 794 20 4717
Fragments arranged by size:
4717 794 346 209 29 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6115
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6080
DraI TTT'AAA
Cuts at: 3419 3419
Size: 6115
DraIII CAC_nnn'GTG
Cuts at: 2521 2977 2521
Size: 456 5659
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5122 809
Size: 507 3806 1802
Fragments arranged by size:
3806 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2884 3174 3562
Size: 54 1425 50 275 18 1054 290 388
Cuts at: 3562 4451 6080 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1054 889 388 290 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2884 3562 1537
Size: 275 1072 678 4090
Fragments arranged by size:
4090 1072 678 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2366 2756 2905 3173 3399 4045 1353
Size: 1013 390 149 268 226 646 3423
Fragments arranged by size:
3423 1013 646 390 268 226 149
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2446 4702 5076 5222 387
Size: 1064 138 857 2256 374 146 1280
Fragments arranged by size:
2256 1280 1064 857 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2520 3005 4178 4775 5562 5830
Size: 393 572 316 485 1173 597 787 268
Cuts at: 5830 1239
Size: 1524
Fragments arranged by size:
1524 1173 787 597 572 485 393 316
268
EcoO109I rG'GnC_Cy
Cuts at: 2229 2956 2229
Size: 727 5388
EcoRI G'AATT_C
Cuts at: 3424 3802 3424
Size: 378 5737
FspI TGC'GCA
Cuts at: 2873 4621 2873
Size: 1748 4367
HaeII r_GCGC'y
Cuts at: 1678 2214 2225 5262 1678
Size: 536 11 3037 2531
Fragments arranged by size:
3037 2531 536 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4756 4788 4830 4862 1373
Size: 32 135 32 3184 32 42 32 2626
Fragments arranged by size:
3184 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3459 3705 669
Size: 988 281 1521 246 3079
Fragments arranged by size:
3079 1521 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6115
HpaI GTT'AAC
Cuts at: 3705 3705
Size: 6115
KpnI G_GTAC'C
Cuts at: 3440 3440
Size: 6115
MfeI C'AATT_G
Cuts at: 3714 3714
Size: 6115
MluI A'CGCG_T
Cuts at: 5991 5991
Size: 6115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2439 5229 5413 1305
Size: 1134 2790 184 2007
Fragments arranged by size:
2790 2007 1134 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3176 10
Size: 54 1425 343 1344 2949
Fragments arranged by size:
2949 1425 1344 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 4073 4432 4591 518
Size: 648 1248 1659 359 159 2042
Fragments arranged by size:
2042 1659 1248 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2037 2242 2251 2273 2299 2475 2883 3099
Size: 431 205 9 22 26 176 408 216
Cuts at: 3099 3107 3351 3375 3531 4208 5356 5601 1606
Size: 8 244 24 156 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 431 408 245 244 216
205 176 156 26 24 22 9 8
NaeI GCC'GGC
Cuts at: 2144 2696 2144
Size: 552 5563
NcoI C'CATG_G
Cuts at: 513 1065 3090 3177 513
Size: 552 2025 87 3451
Fragments arranged by size:
3451 2025 552 87
NdeI CA'TA_TG
Cuts at: 387 3195 387
Size: 2808 3307
NgoMIV G'CCGG_C
Cuts at: 2142 2694 2142
Size: 552 5563
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6115
NotI GC'GGCC_GC
Cuts at: 3562 3562
Size: 6115
NsiI A_TGCA'T
Cuts at: 3120 3120
Size: 6115
NspI r_CATG'y
Cuts at: 1132 1229 3473 5018 1132
Size: 97 2244 1545 2229
Fragments arranged by size:
2244 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6115
PciI A'CATG_T
Cuts at: 5014 5014
Size: 6115
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6115
PmeI GTTT'AAAC
Cuts at: 3419 3419
Size: 6115
PmlI CAC'GTG
Cuts at: 2374 2374
Size: 6115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4184 4216 5697 5729 4184
Size: 32 1481 32 4570
Fragments arranged by size:
4570 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2229 2229
Size: 6115
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6115
PsiI TTA'TAA
Cuts at: 3685 3685
Size: 6115
PspOMI G'GGCC_C
Cuts at: 2956 2956
Size: 6115
PstI C_TGCA'G
Cuts at: 830 2107 3467 830
Size: 1277 1360 3478
Fragments arranged by size:
3478 1360 1277
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6115
PvuII CAG'CTG
Cuts at: 2037 3107 3375 2037
Size: 1070 268 4777
Fragments arranged by size:
4777 1070 268
SacII CC_GC'GG
Cuts at: 2252 2300 2884 2252
Size: 48 584 5483
Fragments arranged by size:
5483 584 48
SalI G'TCGA_C
Cuts at: 1936 3457 1936
Size: 1521 4594
SanDI GG'GwC_CC
Cuts at: 2229 2229
Size: 6115
SapI GCTCTTCn'nnn_
Cuts at: 2366 3399 2366
Size: 1033 5082
SbfI CC_TGCA'GG
Cuts at: 3467 3467
Size: 6115
ScaI AGT'ACT
Cuts at: 4363 6075 4363
Size: 1712 4403
SfcI C'TryA_G
Cuts at: 826 1045 2103 2904 3463 4598 5279 5470 826
Size: 219 1058 801 559 1135 681 191 1471
Fragments arranged by size:
1471 1135 1058 801 681 559 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6115
SgrAI Cr'CCGG_yG
Cuts at: 1700 2093 1700
Size: 393 5722
SmaI CCC'GGG
Cuts at: 1872 2285 3442 1872
Size: 413 1157 4545
Fragments arranged by size:
4545 1157 413
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2013 4219 5120 5382 820
Size: 197 926 70 2206 901 262 1553
Fragments arranged by size:
2206 1553 926 901 262 197 70
SnaBI TAC'GTA
Cuts at: 493 3906 493
Size: 3413 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6115
SphI G_CATG'C
Cuts at: 1132 3473 1132
Size: 2341 3774
SspI AAT'ATT
Cuts at: 5 52 4039 5
Size: 47 3987 2081
Fragments arranged by size:
3987 2081 47
StuI AGG'CCT
Cuts at: 1517 2351 1517
Size: 834 5281
StyI C'CwwG_G
Cuts at: 513 1065 3090 3177 3255 3554 513
Size: 552 2025 87 78 299 3074
Fragments arranged by size:
3074 2025 552 299 87 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2722 3103 3342 4142 4159 4312 4497
Size: 1255 1142 381 239 800 17 153 185
Cuts at: 4497 4942 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1142 800 445 381 239 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4361 6073 96
Size: 274 80 33 51 1443 2384 1712 138
Fragments arranged by size:
2384 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6075 6107 6075
Size: 32 6083
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6115
XbaI T'CTAG_A
Cuts at: 3451 3451
Size: 6115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2232 3336 2232
Size: 1104 5011
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6115
XmaI C'CCGG_G
Cuts at: 1870 2283 3440 1870
Size: 413 1157 4545
Fragments arranged by size:
4545 1157 413
XmnI GAAnn'nnTTC
Cuts at: 4244 4244
Size: 6115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3923 276
Size: 53 83 186 936 2389 2468
Fragments arranged by size:
2468 2389 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI
KasI NarI NruI PacI PfoI PspXI PsrI RsrII
SacI SexAI SfiI SfoI SrfI SwaI