Restriction map for
pFN21AE3195
(Circular) (Six-base) MAPSORT of: pFN21AE3195.seq Check: 5486 from: 1 to: 6079
pFN21AE3195 6079 bp
With 182 enzymes: SgfI *
March 28, 2012 12:52 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3296 3296
Size: 6079
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3889 278
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3422 1937
Size: 1485 4594
Acc65I G'GTAC_C
Cuts at: 3400 3400
Size: 6079
AclI AA'CG_TT
Cuts at: 1563 4206 4579 1563
Size: 2643 373 3063
Fragments arranged by size:
3063 2643 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3180 4142 5526 5794 1632
Size: 1548 962 1384 268 1917
Fragments arranged by size:
1917 1548 1384 962 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5882
AflIII A'CryG_T
Cuts at: 1203 2856 4978 5955 1203
Size: 1653 2122 977 1327
Fragments arranged by size:
2122 1653 1327 977
AgeI A'CCGG_T
Cuts at: 3251 4910 3251
Size: 1659 4420
AhdI GACnn_n'nnGTC
Cuts at: 4808 4808
Size: 6079
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6079
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2265 1184
Size: 1081 4998
AlwNI CAG_nnn'CTG
Cuts at: 3822 5394 3822
Size: 1572 4507
ApaI G_GGCC'C
Cuts at: 2048 2048
Size: 6079
ApaLI G'TGCA_C
Cuts at: 2718 4136 5292 2718
Size: 1418 1156 3505
Fragments arranged by size:
3505 1418 1156
ApoI r'AATT_y
Cuts at: 1493 2469 2630 3388 3614 3766 5875 1493
Size: 976 161 758 226 152 2109 1697
Fragments arranged by size:
2109 1697 976 758 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 4633 160
Size: 872 3601 1606
Fragments arranged by size:
3601 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
AvaI C'yCGr_G
Cuts at: 1870 1943 2166 2378 2450 3404 1870
Size: 73 223 212 72 954 4545
Fragments arranged by size:
4545 954 223 212 73 72
BamHI G'GATC_C
Cuts at: 2424 2898 3409 3776 2424
Size: 474 511 367 4727
Fragments arranged by size:
4727 511 474 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3400 4855 618
Size: 325 217 627 1613 1455 1842
Fragments arranged by size:
1842 1613 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2048 2186 3375 3398 1391
Size: 657 138 1189 23 4072
Fragments arranged by size:
4072 1189 657 138 23
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3129 3168 928
Size: 971 1230 39 3839
Fragments arranged by size:
3839 1230 971 39
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4270 4304 1903
Size: 34 2333 34 3678
Fragments arranged by size:
3678 2333 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3971 5181 3971
Size: 1210 4869
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6079
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4690 136
Size: 107 122 71 631 605 3018 1525
Fragments arranged by size:
3018 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6074 6074
Size: 6079
BlpI GC'TnA_GC
Cuts at: 3496 3496
Size: 6079
Bme1580I G_kGCm'C
Cuts at: 1790 2048 2722 4140 5296 1790
Size: 258 674 1418 1156 2573
Fragments arranged by size:
2573 1418 1156 674 258
BmgBI CAC'GTC
Cuts at: 1318 2434 1318
Size: 1116 4963
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2117 3810 4768
Size: 416 53 83 399 24 700 1693 958
Cuts at: 4768 442
Size: 1753
Fragments arranged by size:
1753 1693 958 700 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6079
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2526 4739 1239
Size: 1287 2213 2579
Fragments arranged by size:
2579 2213 1287
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3344 4204 5069 5367 3344
Size: 860 865 298 4056
Fragments arranged by size:
4056 865 860 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4742 882
Size: 617 3243 2219
Fragments arranged by size:
3243 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3870 493
Size: 711 2666 2702
Fragments arranged by size:
2702 2666 711
BsaBI GATnn'nnATC
Cuts at: 2423 6073 2423
Size: 3650 2429
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3886 4268 4903 275
Size: 53 83 186 936 2353 382 635 1451
Fragments arranged by size:
2353 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2481 3251 4512 4910 5184 5331 5742 2481
Size: 770 1261 398 274 147 411 2818
Fragments arranged by size:
2818 1261 770 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5854
Fragments arranged by size:
5854 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2270 2777 2902 1186
Size: 1084 507 125 4363
Fragments arranged by size:
4363 1084 507 125
BseYI C'CCAG_C
Cuts at: 5282 5778 5282
Size: 496 5583
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 1163
Size: 573 678 4828
Fragments arranged by size:
4828 678 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2030 2136 2323 3529 4290 5318
Size: 275 186 29 106 187 1206 761 1028
Cuts at: 5318 1540
Size: 2301
Fragments arranged by size:
2301 1206 1028 761 275 187 186 106
29
BsiHKAI G_wGCw'C
Cuts at: 2722 3375 4140 4225 5296 2722
Size: 653 765 85 1071 3505
Fragments arranged by size:
3505 1071 765 653 85
BsmI GAATG_Cn'
Cuts at: 3590 3683 3590
Size: 93 5986
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2048 2186 2722 3375 3398 4140 4225
Size: 399 258 138 536 653 23 742 85
Cuts at: 4225 5296 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 653 536 399 258 138
85 23
BspEI T'CCGG_A
Cuts at: 2481 5742 2481
Size: 3261 2818
BspHI T'CATG_A
Cuts at: 3968 5698 3968
Size: 1730 4349
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2358 3296 3434 844
Size: 1514 938 138 3489
Fragments arranged by size:
3489 1514 938 138
BsrBI CCG'CTC
Cuts at: 1394 1823 3966 1394
Size: 429 2143 3507
Fragments arranged by size:
3507 2143 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2529 4574 4748 65
Size: 1183 1281 2045 174 1396
Fragments arranged by size:
2045 1396 1281 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2080 2364 3251 4723 4910 1700
Size: 110 270 284 887 1472 187 2869
Fragments arranged by size:
2869 1472 887 284 270 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6079
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6079
BssSI C'ACGA_G
Cuts at: 4139 5151 4139
Size: 1012 5067
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6079
BstBI TT'CG_AA
Cuts at: 4883 4883
Size: 6079
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6079
BstYI r'GATC_y
Cuts at: 1972 2424 2690 2898 3007 3187 3409 3776 4161
Size: 452 266 208 109 180 222 367 385
Cuts at: 4161 4178 5630 5716 5728 6074 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 452 385 367 346 266 222
208 180 109 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3294 513
Size: 552 939 1290 3298
Fragments arranged by size:
3298 1290 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5340
Fragments arranged by size:
5340 683 56
BtsI GCAGTG_nn'
Cuts at: 3595 4389 4409 3595
Size: 794 20 5265
Fragments arranged by size:
5265 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6079
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6044
DraI TTT'AAA
Cuts at: 3383 3383
Size: 6079
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5086 809
Size: 507 3770 1802
Fragments arranged by size:
3770 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2027 2133 2268
Size: 54 1425 50 275 18 197 106 135
Cuts at: 2268 2553 2872 3246 3526 4415 6044 8
Size: 285 319 374 280 889 1629 43
Fragments arranged by size:
1629 1425 889 374 319 285 280 275
197 135 106 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2027 2133 3526 1537
Size: 275 215 106 1393 4090
Fragments arranged by size:
4090 1393 275 215 106
EarI CTCTTCn'nnn_
Cuts at: 1353 4009 1353
Size: 2656 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4666 5040 5186 387
Size: 1064 138 3077 374 146 1280
Fragments arranged by size:
3077 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3373 3373
Size: 6079
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2526 3180 4142 4739 5526 5794 1239
Size: 393 894 654 962 597 787 268 1524
Fragments arranged by size:
1524 962 894 787 654 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2044 2782 3223 2044
Size: 738 441 4900
Fragments arranged by size:
4900 738 441
EcoRI G'AATT_C
Cuts at: 2630 3388 3766 2630
Size: 758 378 4943
Fragments arranged by size:
4943 758 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2698 2698
Size: 6079
FspI TGC'GCA
Cuts at: 4585 4585
Size: 6079
HaeII r_GCGC'y
Cuts at: 1678 5226 1678
Size: 3548 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4720 4752 4794 4826 1373
Size: 32 135 32 3148 32 42 32 2626
Fragments arranged by size:
3148 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2761 3423 3669 669
Size: 988 281 823 662 246 3079
Fragments arranged by size:
3079 988 823 662 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6079
HpaI GTT'AAC
Cuts at: 3669 3669
Size: 6079
KpnI G_GTAC'C
Cuts at: 3404 3404
Size: 6079
MfeI C'AATT_G
Cuts at: 2511 3678 2511
Size: 1167 4912
MluI A'CGCG_T
Cuts at: 5955 5955
Size: 6079
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2718 5193 5377 1305
Size: 1413 2475 184 2007
Fragments arranged by size:
2475 2007 1413 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2555 2874 10
Size: 54 1425 343 723 319 3215
Fragments arranged by size:
3215 1425 723 343 319 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2436 4037 4396 4555 518
Size: 648 1270 1601 359 159 2042
Fragments arranged by size:
2042 1601 1270 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2032 2551 2707 3349 3495 4172 5320 5565
Size: 426 519 156 642 146 677 1148 245
Cuts at: 5565 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 642 519 426 245 156
146
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4588
Fragments arranged by size:
4588 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6079
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6079
NotI GC'GGCC_GC
Cuts at: 2027 2133 3526 2027
Size: 106 1393 4580
Fragments arranged by size:
4580 1393 106
NspI r_CATG'y
Cuts at: 1132 1229 2193 2329 2682 2860 3437 4982 1132
Size: 97 964 136 353 178 577 1545 2229
Fragments arranged by size:
2229 1545 964 577 353 178 136 97
PasI CC'CwG_GG
Cuts at: 1348 2048 1348
Size: 700 5379
PciI A'CATG_T
Cuts at: 2856 4978 2856
Size: 2122 3957
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6079
PfoI T'CCnGG_A
Cuts at: 2730 3219 2730
Size: 489 5590
PmeI GTTT'AAAC
Cuts at: 3383 3383
Size: 6079
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4148 4180 5661 5693 4148
Size: 32 1481 32 4534
Fragments arranged by size:
4534 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2782 3223 2782
Size: 441 5638
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6079
PsiI TTA'TAA
Cuts at: 3649 3649
Size: 6079
PspOMI G'GGCC_C
Cuts at: 2044 2044
Size: 6079
PstI C_TGCA'G
Cuts at: 830 2355 2948 3431 830
Size: 1525 593 483 3478
Fragments arranged by size:
3478 1525 593 483
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6079
PvuII CAG'CTG
Cuts at: 2707 2707
Size: 6079
SacI G_AGCT'C
Cuts at: 3375 3375
Size: 6079
SalI G'TCGA_C
Cuts at: 1936 3421 1936
Size: 1485 4594
SbfI CC_TGCA'GG
Cuts at: 3431 3431
Size: 6079
ScaI AGT'ACT
Cuts at: 2530 4327 6039 2530
Size: 1797 1712 2570
Fragments arranged by size:
2570 1797 1712
SfcI C'TryA_G
Cuts at: 826 1045 2017 2351 2944 3427 4562 5243 5434
Size: 219 972 334 593 483 1135 681 191
Cuts at: 5434 826
Size: 1471
Fragments arranged by size:
1471 1135 972 681 593 483 334 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6079
SgrAI Cr'CCGG_yG
Cuts at: 1700 2080 3251 1700
Size: 380 1171 4528
Fragments arranged by size:
4528 1171 380
SmaI CCC'GGG
Cuts at: 1872 3406 1872
Size: 1534 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3359 4183 5084 5346 820
Size: 197 926 1416 824 901 262 1553
Fragments arranged by size:
1553 1416 926 901 824 262 197
SnaBI TAC'GTA
Cuts at: 493 3870 493
Size: 3377 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6079
SphI G_CATG'C
Cuts at: 1132 2193 2329 2682 3437 1132
Size: 1061 136 353 755 3774
Fragments arranged by size:
3774 1061 755 353 136
SspI AAT'ATT
Cuts at: 5 52 2621 4003 5
Size: 47 2569 1382 2081
Fragments arranged by size:
2569 2081 1382 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6079
StyI C'CwwG_G
Cuts at: 513 1065 2004 2271 3518 513
Size: 552 939 267 1247 3074
Fragments arranged by size:
3074 1247 939 552 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2785 4106 4123 4276 4461 4906 325
Size: 1255 1205 1321 17 153 185 445 1498
Fragments arranged by size:
1498 1321 1255 1205 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2528 4325 6037
Size: 274 80 33 51 1443 551 1797 1712
Cuts at: 6037 96
Size: 138
Fragments arranged by size:
1797 1712 1443 551 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2712 2744 6039 6071 2712
Size: 32 3295 32 2720
Fragments arranged by size:
3295 2720 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2747 1316
Size: 1431 4648
XbaI T'CTAG_A
Cuts at: 3415 3415
Size: 6079
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6079
XmaI C'CCGG_G
Cuts at: 1870 3404 1870
Size: 1534 4545
XmnI GAAnn'nnTTC
Cuts at: 4208 4208
Size: 6079
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3887 276
Size: 53 83 186 936 2353 2468
Fragments arranged by size:
2468 2353 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI