Restriction map for
pFN21AE3184
(Circular) (Six-base) MAPSORT of: pFN21AE3184.seq Check: 106 from: 1 to: 5971
pFN21AE3184 5971 bp
With 182 enzymes: SgfI *
March 28, 2012 13:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3781 278
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3314 1937
Size: 1377 4594
Acc65I G'GTAC_C
Cuts at: 3292 3292
Size: 5971
AclI AA'CG_TT
Cuts at: 1563 4098 4471 1563
Size: 2535 373 3063
Fragments arranged by size:
3063 2535 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2419 4034 5418 5686 1632
Size: 787 1615 1384 268 1917
Fragments arranged by size:
1917 1615 1384 787 268
AflII C'TTAA_G
Cuts at: 820 1017 2726 820
Size: 197 1709 4065
Fragments arranged by size:
4065 1709 197
AflIII A'CryG_T
Cuts at: 1203 4870 5847 1203
Size: 3667 977 1327
Fragments arranged by size:
3667 1327 977
AgeI A'CCGG_T
Cuts at: 4802 4802
Size: 5971
AhdI GACnn_n'nnGTC
Cuts at: 4700 4700
Size: 5971
AleI CACnn'nnGTG
Cuts at: 1166 2354 1166
Size: 1188 4783
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5971
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2550 2582 2550
Size: 32 5939
AlwNI CAG_nnn'CTG
Cuts at: 3714 5286 3714
Size: 1572 4399
ApaLI G'TGCA_C
Cuts at: 3115 4028 5184 3115
Size: 913 1156 3902
Fragments arranged by size:
3902 1156 913
ApoI r'AATT_y
Cuts at: 1493 3280 3506 3658 5767 1493
Size: 1787 226 152 2109 1697
Fragments arranged by size:
2109 1787 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4525 160
Size: 872 3493 1606
Fragments arranged by size:
3493 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
AvaI C'yCGr_G
Cuts at: 1870 1943 3296 1870
Size: 73 1353 4545
Fragments arranged by size:
4545 1353 73
AvrII C'CTAG_G
Cuts at: 3233 3233
Size: 5971
BamHI G'GATC_C
Cuts at: 3301 3668 3301
Size: 367 5604
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3292 4747 618
Size: 325 217 627 1505 1455 1842
Fragments arranged by size:
1842 1505 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2061 2903 3149 3290 1391
Size: 670 842 246 141 4072
Fragments arranged by size:
4072 842 670 246 141
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5000
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4162 4196 1903
Size: 34 2225 34 3678
Fragments arranged by size:
3678 2225 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3863 5073 3863
Size: 1210 4761
BclI T'GATC_A
Cuts at: 1548 2358 1548
Size: 810 5161
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4582 136
Size: 107 122 71 631 605 2910 1525
Fragments arranged by size:
2910 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5966 5966
Size: 5971
BlpI GC'TnA_GC
Cuts at: 3388 3388
Size: 5971
Bme1580I G_kGCm'C
Cuts at: 1790 2869 3092 3119 4032 5188 1790
Size: 1079 223 27 913 1156 2573
Fragments arranged by size:
2573 1156 1079 913 223 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5971
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2474 2872 3702
Size: 416 53 83 399 24 1057 398 830
Cuts at: 3702 4660 442
Size: 958 1753
Fragments arranged by size:
1753 1057 958 830 416 399 398 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5971
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4631 1239
Size: 3392 2579
Bpu10I CC'TnA_GC
Cuts at: 2142 2142
Size: 5971
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4096 4961 5259 4096
Size: 865 298 4808
Fragments arranged by size:
4808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2465 2776 2798 4634 882
Size: 617 966 311 22 1836 2219
Fragments arranged by size:
2219 1836 966 617 311 22
BsaAI yAC'GTr
Cuts at: 493 1204 3082 3762 493
Size: 711 1878 680 2702
Fragments arranged by size:
2702 1878 711 680
BsaBI GATnn'nnATC
Cuts at: 2638 5965 2638
Size: 3327 2644
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2658 3778 4160 4795
Size: 53 83 186 936 1125 1120 382 635
Cuts at: 4795 275
Size: 1451
Fragments arranged by size:
1451 1125 1120 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4404 4802 5076 5223 5634 4404
Size: 398 274 147 411 4741
Fragments arranged by size:
4741 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 1178
Size: 30 165 30 1147 30 4569
Fragments arranged by size:
4569 1147 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2388 2816 3182 1186
Size: 1202 428 366 3975
Fragments arranged by size:
3975 1202 428 366
BseYI C'CCAG_C
Cuts at: 2054 5174 5670 2054
Size: 3120 496 2355
Fragments arranged by size:
3120 2355 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2942 3062 1163
Size: 573 1206 120 4072
Fragments arranged by size:
4072 1206 573 120
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 4182 5210 1540
Size: 275 186 1420 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3119 4032 4117 5188 3119
Size: 913 85 1071 3902
Fragments arranged by size:
3902 1071 913 85
BsmI GAATG_Cn'
Cuts at: 3482 3575 3482
Size: 93 5878
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 2869 2903 3092 3119 3149 3290
Size: 399 271 808 34 189 27 30 141
Cuts at: 3290 4032 4117 5188 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 399 271 189 141
85 34 30 27
BspEI T'CCGG_A
Cuts at: 5634 5634
Size: 5971
BspHI T'CATG_A
Cuts at: 3860 5590 3860
Size: 1730 4241
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3326 844
Size: 2482 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3858 1394
Size: 429 2035 3507
Fragments arranged by size:
3507 2035 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2625 4466 4640 65
Size: 1183 1377 1841 174 1396
Fragments arranged by size:
1841 1396 1377 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2624 4615 4802 1700
Size: 110 814 1991 187 2869
Fragments arranged by size:
2869 1991 814 187 110
BsrGI T'GTAC_A
Cuts at: 96 2812 2829 96
Size: 2716 17 3238
Fragments arranged by size:
3238 2716 17
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5971
BssSI C'ACGA_G
Cuts at: 4031 5043 4031
Size: 1012 4959
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5971
BstBI TT'CG_AA
Cuts at: 2029 4775 2029
Size: 2746 3225
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5971
BstYI r'GATC_y
Cuts at: 1972 2639 3301 3668 4053 4070 5522 5608 5620
Size: 667 662 367 385 17 1452 86 12
Cuts at: 5620 5966 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 667 662 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2117 2117
Size: 5971
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5419
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3194 487
Size: 683 56 1968 3264
Fragments arranged by size:
3264 1968 683 56
BtsI GCAGTG_nn'
Cuts at: 2384 3487 4281 4301 2384
Size: 1103 794 20 4054
Fragments arranged by size:
4054 1103 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5971
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5936
DraI TTT'AAA
Cuts at: 2020 3275 2020
Size: 1255 4716
DraIII CAC_nnn'GTG
Cuts at: 3096 3096
Size: 5971
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4978 809
Size: 507 3662 1802
Fragments arranged by size:
3662 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936
Size: 54 1425 50 275 18 1588 889 1629
Cuts at: 5936 8
Size: 43
Fragments arranged by size:
1629 1588 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3418 1537
Size: 275 1606 4090
Fragments arranged by size:
4090 1606 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3233 3901 1353
Size: 1880 668 3423
Fragments arranged by size:
3423 1880 668
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4558 4932 5078 387
Size: 1064 138 2969 374 146 1280
Fragments arranged by size:
2969 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2419 4034 4631 5418 5686 1239
Size: 393 787 1615 597 787 268 1524
Fragments arranged by size:
1615 1524 787 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2771 2771
Size: 5971
EcoRI G'AATT_C
Cuts at: 3280 3658 3280
Size: 378 5593
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2841 2841
Size: 5971
FspI TGC'GCA
Cuts at: 4477 4477
Size: 5971
HaeII r_GCGC'y
Cuts at: 1678 5118 1678
Size: 3440 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4612 4644 4686 4718 1373
Size: 32 135 32 3040 32 42 32 2626
Fragments arranged by size:
3040 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3315 3561 669
Size: 988 281 1377 246 3079
Fragments arranged by size:
3079 1377 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5971
HpaI GTT'AAC
Cuts at: 3561 3561
Size: 5971
KpnI G_GTAC'C
Cuts at: 3296 3296
Size: 5971
MfeI C'AATT_G
Cuts at: 3570 3570
Size: 5971
MluI A'CGCG_T
Cuts at: 5847 5847
Size: 5971
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2545 5085 5269 1305
Size: 1240 2540 184 2007
Fragments arranged by size:
2540 2007 1240 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4149
Fragments arranged by size:
4149 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2285 2354 3929 4288 4447 518
Size: 648 843 276 69 1575 359 159 2042
Fragments arranged by size:
2042 1575 843 648 359 276 159 69
MspA1I CmG'CkG
Cuts at: 1606 2054 2926 3387 4064 5212 5457 1606
Size: 448 872 461 677 1148 245 2120
Fragments arranged by size:
2120 1148 872 677 461 448 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5419
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5971
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5971
NotI GC'GGCC_GC
Cuts at: 3418 3418
Size: 5971
NspI r_CATG'y
Cuts at: 1132 1229 3329 4874 1132
Size: 97 2100 1545 2229
Fragments arranged by size:
2229 2100 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5971
PciI A'CATG_T
Cuts at: 4870 4870
Size: 5971
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5971
PfoI T'CCnGG_A
Cuts at: 2331 3129 2331
Size: 798 5173
PmeI GTTT'AAAC
Cuts at: 3275 3275
Size: 5971
PmlI CAC'GTG
Cuts at: 3082 3082
Size: 5971
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2550 2582 3237 3269 4040 4072 5553 5585 2550
Size: 32 655 32 771 32 1481 32 2936
Fragments arranged by size:
2936 1481 771 655 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2771 2771
Size: 5971
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5971
PsiI TTA'TAA
Cuts at: 3041 3541 3041
Size: 500 5471
PstI C_TGCA'G
Cuts at: 830 2215 2391 3219 3323 830
Size: 1385 176 828 104 3478
Fragments arranged by size:
3478 1385 828 176 104
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5971
PvuII CAG'CTG
Cuts at: 2054 2926 2054
Size: 872 5099
SalI G'TCGA_C
Cuts at: 1936 3313 1936
Size: 1377 4594
SbfI CC_TGCA'GG
Cuts at: 3323 3323
Size: 5971
ScaI AGT'ACT
Cuts at: 2675 4219 5931 2675
Size: 1544 1712 2715
Fragments arranged by size:
2715 1712 1544
SfcI C'TryA_G
Cuts at: 826 1045 2049 2211 2387 2775 3045 3215 3319
Size: 219 1004 162 176 388 270 170 104
Cuts at: 3319 4454 5135 5326 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 1004 681 388 270 219 191
176 170 162 104
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5971
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5971
SmaI CCC'GGG
Cuts at: 1872 3298 1872
Size: 1426 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2726 4075 4976 5238 820
Size: 197 926 783 1349 901 262 1553
Fragments arranged by size:
1553 1349 926 901 783 262 197
SnaBI TAC'GTA
Cuts at: 493 3762 493
Size: 3269 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5971
SphI G_CATG'C
Cuts at: 1132 3329 1132
Size: 2197 3774
SspI AAT'ATT
Cuts at: 5 52 2841 3895 5
Size: 47 2789 1054 2081
Fragments arranged by size:
2789 2081 1054 47
StuI AGG'CCT
Cuts at: 1517 2821 1517
Size: 1304 4667
StyI C'CwwG_G
Cuts at: 513 1065 3233 3410 513
Size: 552 2168 177 3074
Fragments arranged by size:
3074 2168 552 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3281 3998 4015 4168 4353 4798 325
Size: 1255 1701 717 17 153 185 445 1498
Fragments arranged by size:
1701 1498 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2673 2812 2829
Size: 274 80 33 51 1443 696 139 17
Cuts at: 2829 4217 5929 96
Size: 1388 1712 138
Fragments arranged by size:
1712 1443 1388 696 274 139 138 80
51 33 17
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5931 5963 5931
Size: 32 5939
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5971
XbaI T'CTAG_A
Cuts at: 3307 3307
Size: 5971
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2090 2329 3079 2090
Size: 239 750 4982
Fragments arranged by size:
4982 750 239
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5971
XmaI C'CCGG_G
Cuts at: 1870 3296 1870
Size: 1426 4545
XmnI GAAnn'nnTTC
Cuts at: 2991 4100 2991
Size: 1109 4862
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3779 276
Size: 53 83 186 936 2245 2468
Fragments arranged by size:
2468 2245 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI