Restriction map for
pFN21AE3181
(Circular) (Six-base) MAPSORT of: pFN21AE3181.seq Check: 3201 from: 1 to: 5959
pFN21AE3181 5959 bp
With 182 enzymes: SgfI *
March 28, 2012 13:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3769 278
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2318 3302 1937
Size: 381 984 4594
Fragments arranged by size:
4594 984 381
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 4858 5835 1203
Size: 3655 977 1327
Fragments arranged by size:
3655 1327 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5959
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AlwNI CAG_nnn'CTG
Cuts at: 3702 5274 3702
Size: 1572 4387
ApaI G_GGCC'C
Cuts at: 2257 2257
Size: 5959
ApaLI G'TGCA_C
Cuts at: 3198 4016 5172 3198
Size: 818 1156 3985
Fragments arranged by size:
3985 1156 818
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2250 3284 1870
Size: 73 307 1034 4545
Fragments arranged by size:
4545 1034 307 73
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3280 4735 618
Size: 325 217 627 1493 1455 1842
Fragments arranged by size:
1842 1493 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2257 3278 1391
Size: 866 1021 4072
Fragments arranged by size:
4072 1021 866
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2531 2607 928
Size: 971 632 76 4280
Fragments arranged by size:
4280 971 632 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2829 2863 4150 4184 1903
Size: 34 892 34 1287 34 3678
Fragments arranged by size:
3678 1287 892 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 2017 1548
Size: 469 5490
BfrBI ATG'CAT
Cuts at: 2578 2578
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5954 5954
Size: 5959
BlpI GC'TnA_GC
Cuts at: 2676 3376 2676
Size: 700 5259
Bme1580I G_kGCm'C
Cuts at: 1790 2257 2852 3202 4020 5176 1790
Size: 467 595 350 818 1156 2573
Fragments arranged by size:
2573 1156 818 595 467 350
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2971 3690 4648
Size: 416 53 83 399 24 1554 719 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1554 958 719 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4619 1239
Size: 3380 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4084 4949 5247 4084
Size: 865 298 4796
Fragments arranged by size:
4796 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2091 4622 882
Size: 617 592 2531 2219
Fragments arranged by size:
2531 2219 617 592
BsaAI yAC'GTr
Cuts at: 493 1204 2660 3750 493
Size: 711 1456 1090 2702
Fragments arranged by size:
2702 1456 1090 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3072 3766 4148 4783
Size: 53 83 186 936 1539 694 382 635
Cuts at: 4783 275
Size: 1451
Fragments arranged by size:
1539 1451 936 694 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2104 2134 1178
Size: 30 165 30 701 30 5003
Fragments arranged by size:
5003 701 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2181 1186
Size: 995 4964
BseYI C'CCAG_C
Cuts at: 2165 5162 5658 2165
Size: 2997 496 2466
Fragments arranged by size:
2997 2466 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5386
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2318 3409 4170 5198 1540
Size: 275 186 317 1091 761 1028 2301
Fragments arranged by size:
2301 1091 1028 761 317 275 186
BsiHKAI G_wGCw'C
Cuts at: 3202 4020 4105 5176 3202
Size: 818 85 1071 3985
Fragments arranged by size:
3985 1071 818 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2257 2852 3202 3278 4020 4105 5176
Size: 399 467 595 350 76 742 85 1071
Cuts at: 5176 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 595 467 399 350 85
76
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 2281 3848 5578 2281
Size: 1567 1730 2662
Fragments arranged by size:
2662 1730 1567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3314 844
Size: 2470 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2593 4454 4628 65
Size: 1183 1345 1861 174 1396
Fragments arranged by size:
1861 1396 1345 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2313 3074 4603 4790 1700
Size: 110 503 761 1529 187 2869
Fragments arranged by size:
2869 1529 761 503 187 110
BsrGI T'GTAC_A
Cuts at: 96 2944 96
Size: 2848 3111
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5959
BssSI C'ACGA_G
Cuts at: 4019 5031 4019
Size: 1012 4947
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5959
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstXI CCAn_nnnn'nTGG
Cuts at: 2948 2948
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 3147 3289 3656 4041 4058 5510 5596 5608
Size: 1175 142 367 385 17 1452 86 12
Cuts at: 5608 5954 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1175 385 367 346 142 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2145 2193 2145
Size: 48 5911
BtgI C'CryG_G
Cuts at: 513 1065 2918 513
Size: 552 1853 3554
Fragments arranged by size:
3554 1853 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5220
Fragments arranged by size:
5220 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5924
DraI TTT'AAA
Cuts at: 2137 3263 2137
Size: 1126 4833
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2311 2938 3406
Size: 54 1425 50 275 18 481 627 468
Cuts at: 3406 4295 5924 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 627 481 468 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2882 3889 1353
Size: 1529 1007 3423
Fragments arranged by size:
3423 1529 1007
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4546 4920 5066 387
Size: 1064 138 2957 374 146 1280
Fragments arranged by size:
2957 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4022 4619 5406 5674 1239
Size: 393 2390 597 787 268 1524
Fragments arranged by size:
2390 1524 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 3248 3248
Size: 5959
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 4465 4465
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 5106 1678
Size: 3428 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2372 2404 2811 2843 4600
Size: 32 135 32 800 32 407 32 1757
Cuts at: 4600 4632 4674 4706 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1757 800 407 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2319 3303 3549 669
Size: 988 281 381 984 246 3079
Fragments arranged by size:
3079 988 984 381 281 246
HindIII A'AGCT_T
Cuts at: 748 2811 748
Size: 2063 3896
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 5835 5835
Size: 5959
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3107 5073 5257 1305
Size: 1802 1966 184 2007
Fragments arranged by size:
2007 1966 1802 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4137
Fragments arranged by size:
4137 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2297 2758 2946 3917 4276 4435 518
Size: 648 1131 461 188 971 359 159 2042
Fragments arranged by size:
2042 1131 971 648 461 359 188 159
MspA1I CmG'CkG
Cuts at: 1606 3375 4052 5200 5445 1606
Size: 1769 677 1148 245 2120
Fragments arranged by size:
2120 1769 1148 677 245
NaeI GCC'GGC
Cuts at: 3076 3076
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 2918 513
Size: 552 1853 3554
Fragments arranged by size:
3554 1853 552
NdeI CA'TA_TG
Cuts at: 387 2429 387
Size: 2042 3917
NgoMIV G'CCGG_C
Cuts at: 3074 3074
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NsiI A_TGCA'T
Cuts at: 2580 2580
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3113 3317 4862 1132
Size: 97 1884 204 1545 2229
Fragments arranged by size:
2229 1884 1545 204 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5959
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2263 2588 1108
Size: 1155 325 4479
Fragments arranged by size:
4479 1155 325
PfoI T'CCnGG_A
Cuts at: 2359 2359
Size: 5959
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3248 3248
Size: 5959
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5959
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PspOMI G'GGCC_C
Cuts at: 2253 2253
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2423 2473 2773 3311 830
Size: 1593 50 300 538 3478
Fragments arranged by size:
3478 1593 538 300 50
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
SalI G'TCGA_C
Cuts at: 1936 2317 3301 1936
Size: 381 984 4594
Fragments arranged by size:
4594 984 381
SbfI CC_TGCA'GG
Cuts at: 3311 3311
Size: 5959
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2419 2469 2769 2853 3307 4442 5123
Size: 219 1374 50 300 84 454 1135 681
Cuts at: 5123 5314 826
Size: 191 1471
Fragments arranged by size:
1471 1374 1135 681 454 300 219 191
84 50
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 3286 1872
Size: 1414 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4063 4964 5226 820
Size: 197 926 2120 901 262 1553
Fragments arranged by size:
2120 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 2778 1517
Size: 1261 4698
StyI C'CwwG_G
Cuts at: 513 1065 2918 3398 513
Size: 552 1853 480 3074
Fragments arranged by size:
3074 1853 552 480
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2186 2392 2944
Size: 274 80 33 51 1443 209 206 552
Cuts at: 2944 4205 5917 96
Size: 1261 1712 138
Fragments arranged by size:
1712 1443 1261 552 274 209 206 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2679 2711 3051 3083 5919 5951 2679
Size: 32 340 32 2836 32 2687
Fragments arranged by size:
2836 2687 340 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3253 3295 3253
Size: 42 5917
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 3284 1870
Size: 1414 4545
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3767 276
Size: 53 83 186 936 2233 2468
Fragments arranged by size:
2468 2233 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI