Restriction map for
pFN21AE3179
(Circular) (Six-base) MAPSORT of: pFN21AE3179.seq Check: 5135 from: 1 to: 5953
pFN21AE3179 5953 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3763 278
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2197 3296 1937
Size: 260 1099 4594
Fragments arranged by size:
4594 1099 260
Acc65I G'GTAC_C
Cuts at: 3274 3274
Size: 5953
AclI AA'CG_TT
Cuts at: 1563 4080 4453 1563
Size: 2517 373 3063
Fragments arranged by size:
3063 2517 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 3156 4016 5400 5668 1632
Size: 1281 243 860 1384 268 1917
Fragments arranged by size:
1917 1384 1281 860 268 243
AflII C'TTAA_G
Cuts at: 820 1017 2665 820
Size: 197 1648 4108
Fragments arranged by size:
4108 1648 197
AflIII A'CryG_T
Cuts at: 1203 2172 4852 5829 1203
Size: 969 2680 977 1327
Fragments arranged by size:
2680 1327 977 969
AgeI A'CCGG_T
Cuts at: 4784 4784
Size: 5953
AhdI GACnn_n'nnGTC
Cuts at: 4682 4682
Size: 5953
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5953
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5953
AlwNI CAG_nnn'CTG
Cuts at: 3696 5268 3696
Size: 1572 4381
ApaI G_GGCC'C
Cuts at: 2051 2255 2051
Size: 204 5749
ApaLI G'TGCA_C
Cuts at: 4010 5166 4010
Size: 1156 4797
ApoI r'AATT_y
Cuts at: 1493 2267 2411 2709 3262 3488 3640 5749 1493
Size: 774 144 298 553 226 152 2109 1697
Fragments arranged by size:
2109 1697 774 553 298 226 152 144
AseI AT'TA_AT
Cuts at: 160 1032 2513 4507 160
Size: 872 1481 1994 1606
Fragments arranged by size:
1994 1606 1481 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
AvaI C'yCGr_G
Cuts at: 1870 1943 3278 1870
Size: 73 1335 4545
Fragments arranged by size:
4545 1335 73
BamHI G'GATC_C
Cuts at: 3283 3650 3283
Size: 367 5586
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2041 2096 3274 4729 618
Size: 325 217 627 254 55 1178 1455 1842
Fragments arranged by size:
1842 1455 1178 627 325 254 217 55
BanII G_rGCy'C
Cuts at: 1391 2051 2255 3272 1391
Size: 660 204 1017 4072
Fragments arranged by size:
4072 1017 660 204
BbeI G_GCGC'C
Cuts at: 2045 2045
Size: 5953
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2919 928
Size: 971 1020 3962
Fragments arranged by size:
3962 1020 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4144 4178 1903
Size: 34 2207 34 3678
Fragments arranged by size:
3678 2207 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3845 5055 3845
Size: 1210 4743
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5953
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4564 136
Size: 107 122 71 631 605 2892 1525
Fragments arranged by size:
2892 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5948 5948
Size: 5953
BlpI GC'TnA_GC
Cuts at: 3370 3370
Size: 5953
Bme1580I G_kGCm'C
Cuts at: 1790 2051 2099 2148 2172 2255 4014 5170 1790
Size: 261 48 49 24 83 1759 1156 2573
Fragments arranged by size:
2573 1759 1156 261 83 49 48 24
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5953
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3684 4642 442
Size: 416 53 83 399 24 2267 958 1753
Fragments arranged by size:
2267 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2623 3044 4613 1239
Size: 1384 421 1569 2579
Fragments arranged by size:
2579 1569 1384 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4078 4943 5241 4078
Size: 865 298 4790
Fragments arranged by size:
4790 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4616 882
Size: 617 3117 2219
Fragments arranged by size:
3117 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3744 493
Size: 711 2540 2702
Fragments arranged by size:
2702 2540 711
BsaBI GATnn'nnATC
Cuts at: 5947 5947
Size: 5953
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 3760 4142 4777
Size: 53 83 186 936 509 1718 382 635
Cuts at: 4777 275
Size: 1451
Fragments arranged by size:
1718 1451 936 635 509 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4386 4784 5058 5205 5616 4386
Size: 398 274 147 411 4723
Fragments arranged by size:
4723 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5728
Fragments arranged by size:
5728 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5953
BseYI C'CCAG_C
Cuts at: 5156 5652 5156
Size: 496 5457
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2352 1163
Size: 573 616 4764
Fragments arranged by size:
4764 616 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2079 3403 4164 5192 1540
Size: 275 186 78 1324 761 1028 2301
Fragments arranged by size:
2301 1324 1028 761 275 186 78
BsiHKAI G_wGCw'C
Cuts at: 4014 4099 5170 4014
Size: 85 1071 4797
Fragments arranged by size:
4797 1071 85
BsmI GAATG_Cn'
Cuts at: 3464 3557 3464
Size: 93 5860
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2051 2099 2148 2172 2255 3272 4014
Size: 399 261 48 49 24 83 1017 742
Cuts at: 4014 4099 5170 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 1017 742 399 261 85 83
49 48 24
BspEI T'CCGG_A
Cuts at: 5616 5616
Size: 5953
BspHI T'CATG_A
Cuts at: 3842 5572 3842
Size: 1730 4223
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3308 844
Size: 2464 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3840 1394
Size: 429 2017 3507
Fragments arranged by size:
3507 2017 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4448 4622 65
Size: 1183 3200 174 1396
Fragments arranged by size:
3200 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2071 2107 4597 4784 1700
Size: 110 261 36 2490 187 2869
Fragments arranged by size:
2869 2490 261 187 110 36
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5953
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5953
BssSI C'ACGA_G
Cuts at: 4013 5025 4013
Size: 1012 4941
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5953
BstBI TT'CG_AA
Cuts at: 3235 4757 3235
Size: 1522 4431
BstEII G'GTnAC_C
Cuts at: 1184 2355 1184
Size: 1171 4782
BstYI r'GATC_y
Cuts at: 1972 3283 3650 4035 4052 5504 5590 5602 5948
Size: 1311 367 385 17 1452 86 12 346
Cuts at: 5948 1972
Size: 1977
Fragments arranged by size:
1977 1452 1311 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2198 2198
Size: 5953
Bsu36I CC'TnA_GG
Cuts at: 2091 2091
Size: 5953
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4462
Fragments arranged by size:
4462 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5214
Fragments arranged by size:
5214 683 56
BtsI GCAGTG_nn'
Cuts at: 2364 2723 3469 4263 4283 2364
Size: 359 746 794 20 4034
Fragments arranged by size:
4034 794 746 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5953
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5918
DraI TTT'AAA
Cuts at: 3066 3257 3066
Size: 191 5762
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4960 809
Size: 507 3644 1802
Fragments arranged by size:
3644 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2163 3400 4289
Size: 54 1425 50 275 18 333 1237 889
Cuts at: 4289 5918 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1237 889 333 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3400 1537
Size: 275 1588 4090
Fragments arranged by size:
4090 1588 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2514 3883 1353
Size: 1161 1369 3423
Fragments arranged by size:
3423 1369 1161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4540 4914 5060 387
Size: 1064 138 2951 374 146 1280
Fragments arranged by size:
2951 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2623 2913 3044 3156 4016 4613 5400
Size: 393 991 290 131 112 860 597 787
Cuts at: 5400 5668 1239
Size: 268 1524
Fragments arranged by size:
1524 991 860 787 597 393 290 268
131 112
EcoO109I rG'GnC_Cy
Cuts at: 2048 2251 2048
Size: 203 5750
EcoRI G'AATT_C
Cuts at: 2267 3262 3640 2267
Size: 995 378 4580
Fragments arranged by size:
4580 995 378
FspI TGC'GCA
Cuts at: 4459 4459
Size: 5953
HaeII r_GCGC'y
Cuts at: 1678 2045 5100 1678
Size: 367 3055 2531
Fragments arranged by size:
3055 2531 367
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2006 2038 2539 2571 2621
Size: 32 135 32 434 32 501 32 50
Cuts at: 2621 2653 4594 4626 4668 4700 1373
Size: 32 1941 32 42 32 2626
Fragments arranged by size:
2626 1941 501 434 135 50 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3297 3543 669
Size: 988 281 1359 246 3079
Fragments arranged by size:
3079 1359 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2605 2941 748
Size: 1857 336 3760
Fragments arranged by size:
3760 1857 336
HpaI GTT'AAC
Cuts at: 3543 3543
Size: 5953
KasI G'GCGC_C
Cuts at: 2041 2041
Size: 5953
KpnI G_GTAC'C
Cuts at: 3278 3278
Size: 5953
MfeI C'AATT_G
Cuts at: 3552 3552
Size: 5953
MluI A'CGCG_T
Cuts at: 5829 5829
Size: 5953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5067 5251 1305
Size: 3762 184 2007
Fragments arranged by size:
3762 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4131
Fragments arranged by size:
4131 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2211 3066 3911 4270 4429 518
Size: 648 1045 855 845 359 159 2042
Fragments arranged by size:
2042 1045 855 845 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3369 4046 5194 5439 1606
Size: 1763 677 1148 245 2120
Fragments arranged by size:
2120 1763 1148 677 245
NaeI GCC'GGC
Cuts at: 2109 2109
Size: 5953
NarI GG'CG_CC
Cuts at: 2042 2042
Size: 5953
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4462
Fragments arranged by size:
4462 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5953
NgoMIV G'CCGG_C
Cuts at: 2107 2107
Size: 5953
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5953
NotI GC'GGCC_GC
Cuts at: 3400 3400
Size: 5953
NspI r_CATG'y
Cuts at: 1132 1229 2176 3052 3311 4856 1132
Size: 97 947 876 259 1545 2229
Fragments arranged by size:
2229 1545 947 876 259 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5953
PciI A'CATG_T
Cuts at: 2172 4852 2172
Size: 2680 3273
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5953
PmeI GTTT'AAAC
Cuts at: 3257 3257
Size: 5953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2513 2545 4022 4054 5535 5567 2513
Size: 32 1477 32 1481 32 2899
Fragments arranged by size:
2899 1481 1477 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2851 1613
Size: 1238 4715
PsiI TTA'TAA
Cuts at: 3523 3523
Size: 5953
PspOMI G'GGCC_C
Cuts at: 2047 2251 2047
Size: 204 5749
PstI C_TGCA'G
Cuts at: 830 2718 3305 830
Size: 1888 587 3478
Fragments arranged by size:
3478 1888 587
PvuI CG_AT'CG
Cuts at: 2001 2079 2001
Size: 78 5875
SalI G'TCGA_C
Cuts at: 1936 3295 1936
Size: 1359 4594
SbfI CC_TGCA'GG
Cuts at: 3305 3305
Size: 5953
ScaI AGT'ACT
Cuts at: 2868 4201 5913 2868
Size: 1333 1712 2908
Fragments arranged by size:
2908 1712 1333
SfcI C'TryA_G
Cuts at: 826 1045 2714 3301 4436 5117 5308 826
Size: 219 1669 587 1135 681 191 1471
Fragments arranged by size:
1669 1471 1135 681 587 219 191
SfoI GGC'GCC
Cuts at: 2043 2043
Size: 5953
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5953
SgrAI Cr'CCGG_yG
Cuts at: 1700 2071 1700
Size: 371 5582
SmaI CCC'GGG
Cuts at: 1872 3280 1872
Size: 1408 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2665 4057 4958 5220 820
Size: 197 926 722 1392 901 262 1553
Fragments arranged by size:
1553 1392 926 901 722 262 197
SnaBI TAC'GTA
Cuts at: 493 3744 493
Size: 3251 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5953
SphI G_CATG'C
Cuts at: 1132 3311 1132
Size: 2179 3774
SspI AAT'ATT
Cuts at: 5 52 2330 2342 3877 5
Size: 47 2278 12 1535 2081
Fragments arranged by size:
2278 2081 1535 47 12
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5953
StyI C'CwwG_G
Cuts at: 513 1065 2004 2246 3392 513
Size: 552 939 242 1146 3074
Fragments arranged by size:
3074 1146 939 552 242
SwaI ATTT'AAAT
Cuts at: 3066 3066
Size: 5953
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3027 3980 3997 4150 4335 4780 325
Size: 1255 1447 953 17 153 185 445 1498
Fragments arranged by size:
1498 1447 1255 953 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2866 2874 3076
Size: 274 80 33 51 1443 889 8 202
Cuts at: 3076 3229 4199 5911 96
Size: 153 970 1712 138
Fragments arranged by size:
1712 1443 970 889 274 202 153 138
80 51 33 8
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5913 5945 5913
Size: 32 5921
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5953
XbaI T'CTAG_A
Cuts at: 2314 3289 2314
Size: 975 4978
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3113 3113
Size: 5953
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5953
XmaI C'CCGG_G
Cuts at: 1870 3278 1870
Size: 1408 4545
XmnI GAAnn'nnTTC
Cuts at: 4082 4082
Size: 5953
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3761 276
Size: 53 83 186 936 2227 2468
Fragments arranged by size:
2468 2227 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI SwaI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI NruI NsiI PacI PfoI
PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI