Restriction map for
pFN21AE3176
(Circular) (Six-base) MAPSORT of: pFN21AE3176.seq Check: 898 from: 1 to: 5935
pFN21AE3176 5935 bp
With 182 enzymes: SgfI *
March 28, 2012 12:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3745 278
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3278 1937
Size: 1341 4594
Acc65I G'GTAC_C
Cuts at: 2204 3256 2204
Size: 1052 4883
AclI AA'CG_TT
Cuts at: 1563 4062 4435 1563
Size: 2499 373 3063
Fragments arranged by size:
3063 2499 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 3998 5382 5650 1632
Size: 1040 1326 1384 268 1917
Fragments arranged by size:
1917 1384 1326 1040 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5738
AflIII A'CryG_T
Cuts at: 1203 4834 5811 1203
Size: 3631 977 1327
Fragments arranged by size:
3631 1327 977
AgeI A'CCGG_T
Cuts at: 4766 4766
Size: 5935
AhdI GACnn_n'nnGTC
Cuts at: 4664 4664
Size: 5935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5935
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5935
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2135 2167 2938 2970 2135
Size: 32 771 32 5100
Fragments arranged by size:
5100 771 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2364 3173 3678 5250 2364
Size: 809 505 1572 3049
Fragments arranged by size:
3049 1572 809 505
ApaI G_GGCC'C
Cuts at: 2929 2929
Size: 5935
ApaLI G'TGCA_C
Cuts at: 2216 3992 5148 2216
Size: 1776 1156 3003
Fragments arranged by size:
3003 1776 1156
ApoI r'AATT_y
Cuts at: 1493 3244 3470 3622 5731 1493
Size: 1751 226 152 2109 1697
Fragments arranged by size:
2109 1751 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4489 160
Size: 872 3457 1606
Fragments arranged by size:
3457 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 3260 1870
Size: 73 196 1121 4545
Fragments arranged by size:
4545 1121 196 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2188 2221 2188
Size: 33 5902
BamHI G'GATC_C
Cuts at: 3207 3265 3632 3207
Size: 58 367 5510
Fragments arranged by size:
5510 367 58
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2204 3256 4711 618
Size: 325 217 627 417 1052 1455 1842
Fragments arranged by size:
1842 1455 1052 627 417 325 217
BanII G_rGCy'C
Cuts at: 1391 2064 2286 2420 2448 2898 2907 2929 3022
Size: 673 222 134 28 450 9 22 93
Cuts at: 3022 3254 1391
Size: 232 4072
Fragments arranged by size:
4072 673 450 232 222 134 93 28
22 9
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2487 928
Size: 971 588 4376
Fragments arranged by size:
4376 971 588
BbvCI CC'TCA_GC
Cuts at: 2734 2734
Size: 5935
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2113 2147 4126 4160 1903
Size: 34 176 34 1979 34 3678
Fragments arranged by size:
3678 1979 176 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3827 5037 3827
Size: 1210 4725
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5935
BfrBI ATG'CAT
Cuts at: 2684 2684
Size: 5935
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2396 4546 136
Size: 107 122 71 631 605 724 2150 1525
Fragments arranged by size:
2150 1525 724 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5930 5930
Size: 5935
BlpI GC'TnA_GC
Cuts at: 3352 3352
Size: 5935
Bme1580I G_kGCm'C
Cuts at: 1790 2088 2220 2929 3996 5152 1790
Size: 298 132 709 1067 1156 2573
Fragments arranged by size:
2573 1156 1067 709 298 132
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5935
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2258 2371 3666
Size: 416 53 83 399 24 841 113 1295
Cuts at: 3666 4624 442
Size: 958 1753
Fragments arranged by size:
1753 1295 958 841 416 399 113 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5935
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2318 2445 2921 4595 1239
Size: 839 240 127 476 1674 2579
Fragments arranged by size:
2579 1674 839 476 240 127
Bpu10I CC'TnA_GC
Cuts at: 2734 2734
Size: 5935
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4060 4925 5223 4060
Size: 865 298 4772
Fragments arranged by size:
4772 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2299 3086 4598 882
Size: 617 800 787 1512 2219
Fragments arranged by size:
2219 1512 800 787 617
BsaAI yAC'GTr
Cuts at: 493 1204 3726 493
Size: 711 2522 2702
Fragments arranged by size:
2702 2522 711
BsaBI GATnn'nnATC
Cuts at: 5929 5929
Size: 5935
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3742 4124 4759 275
Size: 53 83 186 936 2209 382 635 1451
Fragments arranged by size:
2209 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2886 4368 4766 5040 5187 5598 2886
Size: 1482 398 274 147 411 3223
Fragments arranged by size:
3223 1482 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2135 2165 2938 2968 1178
Size: 30 165 30 732 30 773 30 4145
Fragments arranged by size:
4145 773 732 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2705 2921 1186
Size: 1519 216 4200
Fragments arranged by size:
4200 1519 216
BseYI C'CCAG_C
Cuts at: 2391 5138 5634 2391
Size: 2747 496 2692
Fragments arranged by size:
2747 2692 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2054 2072 2350 2897 1163
Size: 573 318 18 278 547 4201
Fragments arranged by size:
4201 573 547 318 278 18
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3385 4146 5174 1540
Size: 275 186 1384 761 1028 2301
Fragments arranged by size:
2301 1384 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2220 2567 2732 2898 3150 3996 4081 5152 2220
Size: 347 165 166 252 846 85 1071 3003
Fragments arranged by size:
3003 1071 846 347 252 166 165 85
BsmI GAATG_Cn'
Cuts at: 3446 3539 3446
Size: 93 5842
BsmBI CGTCTCn'nnnn_
Cuts at: 2140 2140
Size: 5935
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2088 2220 2286 2420 2448 2567
Size: 399 274 24 132 66 134 28 119
Cuts at: 2567 2732 2898 2907 2929 3022 3150 3254 3996
Size: 165 166 9 22 93 128 104 742
Cuts at: 3996 4081 5152 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 274 166 165 134
132 128 119 104 93 85 66 28
24 22 9
BspEI T'CCGG_A
Cuts at: 2886 5598 2886
Size: 2712 3223
BspHI T'CATG_A
Cuts at: 3824 5554 3824
Size: 1730 4205
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2758 3290 844
Size: 1914 532 3489
Fragments arranged by size:
3489 1914 532
BsrBI CCG'CTC
Cuts at: 1394 1823 3822 1394
Size: 429 1999 3507
Fragments arranged by size:
3507 1999 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2359 2540 4430 4604 65
Size: 1183 1111 181 1890 174 1396
Fragments arranged by size:
1890 1396 1183 1111 181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2345 4579 4766 1700
Size: 110 535 2234 187 2869
Fragments arranged by size:
2869 2234 535 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5935
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5935
BssSI C'ACGA_G
Cuts at: 3041 3995 5007 3041
Size: 954 1012 3969
Fragments arranged by size:
3969 1012 954
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2364 794
Size: 1570 4365
BstBI TT'CG_AA
Cuts at: 4739 4739
Size: 5935
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5935
BstYI r'GATC_y
Cuts at: 1972 2647 3207 3265 3632 4017 4034 5486 5572
Size: 675 560 58 367 385 17 1452 86
Cuts at: 5572 5584 5930 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 675 560 385 367 346 86
58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2522 3061 513
Size: 552 939 518 539 3387
Fragments arranged by size:
3387 939 552 539 518
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2691 487
Size: 683 56 1465 3731
Fragments arranged by size:
3731 1465 683 56
BtsI GCAGTG_nn'
Cuts at: 3451 4245 4265 3451
Size: 794 20 5121
Fragments arranged by size:
5121 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5935
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5900
DraI TTT'AAA
Cuts at: 3239 3239
Size: 5935
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4942 809
Size: 507 3626 1802
Fragments arranged by size:
3626 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2316 2381
Size: 54 1425 50 275 18 342 144 65
Cuts at: 2381 2421 3382 4271 5900 8
Size: 40 961 889 1629 43
Fragments arranged by size:
1629 1425 961 889 342 275 144 65
54 50 43 40 18
EagI C'GGCC_G
Cuts at: 1537 1812 3382 1537
Size: 275 1570 4090
Fragments arranged by size:
4090 1570 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2436 2504 3865 1353
Size: 1083 68 1361 3423
Fragments arranged by size:
3423 1361 1083 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4522 4896 5042 387
Size: 1064 138 2933 374 146 1280
Fragments arranged by size:
2933 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2896 2896
Size: 5935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2318 2445 2672 2921 3998 4595
Size: 393 446 240 127 227 249 1077 597
Cuts at: 4595 5382 5650 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1077 787 597 446 393 268 249
240 227 127
EcoNI CCTnn'n_nnAGG
Cuts at: 2072 2072
Size: 5935
EcoO109I rG'GnC_Cy
Cuts at: 2527 2661 2926 2527
Size: 134 265 5536
Fragments arranged by size:
5536 265 134
EcoRI G'AATT_C
Cuts at: 3244 3622 3244
Size: 378 5557
EcoRV GAT'ATC
Cuts at: 3220 3220
Size: 5935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2809 2809
Size: 5935
FspI TGC'GCA
Cuts at: 2724 2753 4441 2724
Size: 29 1688 4218
Fragments arranged by size:
4218 1688 29
FspAI rTGC'GCAy
Cuts at: 2753 2753
Size: 5935
HaeII r_GCGC'y
Cuts at: 1678 5082 1678
Size: 3404 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2669 2701 2838 2870 4576
Size: 32 135 32 1097 32 137 32 1706
Cuts at: 4576 4608 4650 4682 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1706 1097 137 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3094 3279 3525 669
Size: 988 281 1156 185 246 3079
Fragments arranged by size:
3079 1156 988 281 246 185
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5935
HpaI GTT'AAC
Cuts at: 3525 3525
Size: 5935
KpnI G_GTAC'C
Cuts at: 2208 3260 2208
Size: 1052 4883
MfeI C'AATT_G
Cuts at: 3534 3534
Size: 5935
MluI A'CGCG_T
Cuts at: 5811 5811
Size: 5935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5049 5233 1305
Size: 3744 184 2007
Fragments arranged by size:
3744 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2318 10
Size: 54 1425 343 342 144 3627
Fragments arranged by size:
3627 1425 343 342 144 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2093 2637 2676 3101 3893 4252
Size: 648 843 84 544 39 425 792 359
Cuts at: 4252 4411 518
Size: 159 2042
Fragments arranged by size:
2042 843 792 648 544 425 359 159
84 39
MspA1I CmG'CkG
Cuts at: 1606 2056 2395 3351 4028 5176 5421 1606
Size: 450 339 956 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 677 450 339 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4444
Fragments arranged by size:
4444 939 552
NdeI CA'TA_TG
Cuts at: 387 2321 387
Size: 1934 4001
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5935
NotI GC'GGCC_GC
Cuts at: 3382 3382
Size: 5935
NsiI A_TGCA'T
Cuts at: 2686 2686
Size: 5935
NspI r_CATG'y
Cuts at: 1132 1229 2390 2767 3293 4838 1132
Size: 97 1161 377 526 1545 2229
Fragments arranged by size:
2229 1545 1161 526 377 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5935
PciI A'CATG_T
Cuts at: 4834 4834
Size: 5935
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3173 1108
Size: 2065 3870
PfoI T'CCnGG_A
Cuts at: 2643 2898 2643
Size: 255 5680
PmeI GTTT'AAAC
Cuts at: 3239 3239
Size: 5935
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2135 2167 2938 2970 4004 4036 5517 5549 2135
Size: 32 771 32 1034 32 1481 32 2521
Fragments arranged by size:
2521 1481 1034 771 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2661 2661
Size: 5935
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5935
PsiI TTA'TAA
Cuts at: 3505 3505
Size: 5935
PspOMI G'GGCC_C
Cuts at: 2925 2925
Size: 5935
PstI C_TGCA'G
Cuts at: 830 3216 3287 830
Size: 2386 71 3478
Fragments arranged by size:
3478 2386 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5935
PvuII CAG'CTG
Cuts at: 2056 2395 2056
Size: 339 5596
SacI G_AGCT'C
Cuts at: 2898 2898
Size: 5935
SalI G'TCGA_C
Cuts at: 1936 3277 1936
Size: 1341 4594
SanDI GG'GwC_CC
Cuts at: 2661 2661
Size: 5935
SapI GCTCTTCn'nnn_
Cuts at: 2436 2504 2436
Size: 68 5867
SbfI CC_TGCA'GG
Cuts at: 3216 3287 3216
Size: 71 5864
ScaI AGT'ACT
Cuts at: 2792 2813 4183 5895 2792
Size: 21 1370 1712 2832
Fragments arranged by size:
2832 1712 1370 21
SexAI A'CCwGG_T
Cuts at: 2073 2496 2073
Size: 423 5512
SfcI C'TryA_G
Cuts at: 826 1045 3212 3283 4418 5099 5290 826
Size: 219 2167 71 1135 681 191 1471
Fragments arranged by size:
2167 1471 1135 681 219 191 71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5935
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5935
SmaI CCC'GGG
Cuts at: 1872 2141 3262 1872
Size: 269 1121 4545
Fragments arranged by size:
4545 1121 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4039 4940 5202 820
Size: 197 926 2096 901 262 1553
Fragments arranged by size:
2096 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3726 493
Size: 3233 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5935
SphI G_CATG'C
Cuts at: 1132 2390 2767 3293 1132
Size: 1258 377 526 3774
Fragments arranged by size:
3774 1258 526 377
SspI AAT'ATT
Cuts at: 5 52 3859 5
Size: 47 3807 2081
Fragments arranged by size:
3807 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5935
StyI C'CwwG_G
Cuts at: 513 1065 2004 2409 2424 3087 3374 513
Size: 552 939 405 15 663 287 3074
Fragments arranged by size:
3074 939 663 552 405 287 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2422 2735 3962 3979 4132 4317 4762
Size: 1255 842 313 1227 17 153 185 445
Cuts at: 4762 325
Size: 1498
Fragments arranged by size:
1498 1255 1227 842 445 313 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2196 2625 2790
Size: 274 80 33 51 1443 219 429 165
Cuts at: 2790 2811 4181 5893 96
Size: 21 1370 1712 138
Fragments arranged by size:
1712 1443 1370 429 274 219 165 138
80 51 33 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5895 5927 5895
Size: 32 5903
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5935
XbaI T'CTAG_A
Cuts at: 3271 3271
Size: 5935
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5935
XmaI C'CCGG_G
Cuts at: 1870 2139 3260 1870
Size: 269 1121 4545
Fragments arranged by size:
4545 1121 269
XmnI GAAnn'nnTTC
Cuts at: 2183 2513 4064 2183
Size: 330 1551 4054
Fragments arranged by size:
4054 1551 330
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3743 276
Size: 53 83 186 936 2209 2468
Fragments arranged by size:
2468 2209 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BbeI BplI BsiWI BstXI
BstZ17I Bsu36I DraIII FseI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI PsrI RsrII SacII SfiI
SfoI SrfI SwaI XcmI