Restriction map for
pFN21AE3174
(Circular) (Six-base) MAPSORT of: pFN21AE3174.seq Check: 8116 from: 1 to: 5926
pFN21AE3174 5926 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3736 278
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3269 1937
Size: 1332 4594
Acc65I G'GTAC_C
Cuts at: 2604 3247 2604
Size: 643 5283
AclI AA'CG_TT
Cuts at: 1563 4053 4426 1563
Size: 2490 373 3063
Fragments arranged by size:
3063 2490 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2697 2933 3989 5373 5641 1632
Size: 1065 236 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1065 1056 268 236
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5729
AflIII A'CryG_T
Cuts at: 1203 4825 5802 1203
Size: 3622 977 1327
Fragments arranged by size:
3622 1327 977
AgeI A'CCGG_T
Cuts at: 4757 4757
Size: 5926
AhdI GACnn_n'nnGTC
Cuts at: 4655 4655
Size: 5926
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5926
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5926
AlwNI CAG_nnn'CTG
Cuts at: 3669 5241 3669
Size: 1572 4354
ApaLI G'TGCA_C
Cuts at: 3983 5139 3983
Size: 1156 4770
ApoI r'AATT_y
Cuts at: 1493 3235 3461 3613 5722 1493
Size: 1742 226 152 2109 1697
Fragments arranged by size:
2109 1742 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2462 4480 160
Size: 872 1430 2018 1606
Fragments arranged by size:
2018 1606 1430 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 3251 1870
Size: 73 196 1112 4545
Fragments arranged by size:
4545 1112 196 73
BamHI G'GATC_C
Cuts at: 3256 3623 3256
Size: 367 5559
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2604 3247 4702 618
Size: 325 217 627 817 643 1455 1842
Fragments arranged by size:
1842 1455 817 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2892 3118 3245 1391
Size: 1501 226 127 4072
Fragments arranged by size:
4072 1501 226 127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2921 928
Size: 971 1022 3933
Fragments arranged by size:
3933 1022 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4117 4151 1903
Size: 34 50 34 2096 34 3678
Fragments arranged by size:
3678 2096 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2418 3818 5028 2025
Size: 393 1400 1210 2923
Fragments arranged by size:
2923 1400 1210 393
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5926
BfrBI ATG'CAT
Cuts at: 2381 2381
Size: 5926
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4537 136
Size: 107 122 71 631 605 2865 1525
Fragments arranged by size:
2865 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2180 5921 2180
Size: 3741 2185
BlpI GC'TnA_GC
Cuts at: 3196 3343 3196
Size: 147 5779
Bme1580I G_kGCm'C
Cuts at: 1790 2310 3987 5143 1790
Size: 520 1677 1156 2573
Fragments arranged by size:
2573 1677 1156 520
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5926
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3657 4615 442
Size: 416 53 83 399 24 2240 958 1753
Fragments arranged by size:
2240 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5926
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3132 3132
Size: 5926
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2493 4586 1239
Size: 1254 2093 2579
Fragments arranged by size:
2579 2093 1254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2145 2169 4051 4916 5214 2145
Size: 24 1882 865 298 2857
Fragments arranged by size:
2857 1882 865 298 24
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2358 2929 4589 882
Size: 617 859 571 1660 2219
Fragments arranged by size:
2219 1660 859 617 571
BsaAI yAC'GTr
Cuts at: 493 1204 3717 493
Size: 711 2513 2702
Fragments arranged by size:
2702 2513 711
BsaBI GATnn'nnATC
Cuts at: 5920 5920
Size: 5926
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3733 4115 4750 275
Size: 53 83 186 936 2200 382 635 1451
Fragments arranged by size:
2200 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4359 4757 5031 5178 5589 4359
Size: 398 274 147 411 4696
Fragments arranged by size:
4696 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5701
Fragments arranged by size:
5701 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2702 1186
Size: 1516 4410
BseYI C'CCAG_C
Cuts at: 2431 2757 5129 5625 2431
Size: 326 2372 496 2732
Fragments arranged by size:
2732 2372 496 326
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2498 1163
Size: 573 762 4591
Fragments arranged by size:
4591 762 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3376 4137 5165 1540
Size: 275 186 1375 761 1028 2301
Fragments arranged by size:
2301 1375 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2892 3118 3987 4072 5143 2892
Size: 226 869 85 1071 3675
Fragments arranged by size:
3675 1071 869 226 85
BsmI GAATG_Cn'
Cuts at: 2048 2644 3437 3530 2048
Size: 596 793 93 4444
Fragments arranged by size:
4444 793 596 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2310 2892 3118 3245 3987 4072 5143
Size: 399 520 582 226 127 742 85 1071
Cuts at: 5143 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 582 520 399 226 127
85
BspEI T'CCGG_A
Cuts at: 5589 5589
Size: 5926
BspHI T'CATG_A
Cuts at: 3815 5545 3815
Size: 1730 4196
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2592 3281 844
Size: 1748 689 3489
Fragments arranged by size:
3489 1748 689
BsrBI CCG'CTC
Cuts at: 1394 1823 3813 1394
Size: 429 1990 3507
Fragments arranged by size:
3507 1990 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4421 4595 65
Size: 1183 3173 174 1396
Fragments arranged by size:
3173 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4570 4757 1700
Size: 110 2760 187 2869
Fragments arranged by size:
2869 2760 187 110
BsrGI T'GTAC_A
Cuts at: 96 3172 96
Size: 3076 2850
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5926
BssSI C'ACGA_G
Cuts at: 3986 4998 3986
Size: 1012 4914
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5926
BstBI TT'CG_AA
Cuts at: 4730 4730
Size: 5926
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5926
BstYI r'GATC_y
Cuts at: 1972 2180 2509 3256 3623 4008 4025 5477 5563
Size: 208 329 747 367 385 17 1452 86
Cuts at: 5563 5575 5921 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 747 385 367 346 329 208
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5374
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5187
Fragments arranged by size:
5187 683 56
BtsI GCAGTG_nn'
Cuts at: 2619 3442 4236 4256 2619
Size: 823 794 20 4289
Fragments arranged by size:
4289 823 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5926
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5891
DraI TTT'AAA
Cuts at: 3230 3230
Size: 5926
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2943 4933 809
Size: 507 1627 1990 1802
Fragments arranged by size:
1990 1802 1627 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2090 3373 4262
Size: 54 1425 50 275 18 260 1283 889
Cuts at: 4262 5891 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1283 889 275 260 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3373 1537
Size: 275 1561 4090
Fragments arranged by size:
4090 1561 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2871 3106 3856 1353
Size: 1518 235 750 3423
Fragments arranged by size:
3423 1518 750 235
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4513 4887 5033 387
Size: 1064 138 2924 374 146 1280
Fragments arranged by size:
2924 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2890 3116 2890
Size: 226 5700
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2493 2697 2933 3989 4586 5373 5641
Size: 393 861 204 236 1056 597 787 268
Cuts at: 5641 1239
Size: 1524
Fragments arranged by size:
1524 1056 861 787 597 393 268 236
204
EcoNI CCTnn'n_nnAGG
Cuts at: 2079 2547 2079
Size: 468 5458
EcoRI G'AATT_C
Cuts at: 3235 3613 3235
Size: 378 5548
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2063 3135 2063
Size: 1072 4854
FspI TGC'GCA
Cuts at: 4432 4432
Size: 5926
HaeII r_GCGC'y
Cuts at: 1678 3196 5073 1678
Size: 1518 1877 2531
Fragments arranged by size:
2531 1877 1518
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3201 3233 4567 4599 4641
Size: 32 135 32 1629 32 1334 32 42
Cuts at: 4641 4673 1373
Size: 32 2626
Fragments arranged by size:
2626 1629 1334 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3270 3516 669
Size: 988 281 1332 246 3079
Fragments arranged by size:
3079 1332 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3203 748
Size: 2455 3471
HpaI GTT'AAC
Cuts at: 3516 3516
Size: 5926
KpnI G_GTAC'C
Cuts at: 2608 3251 2608
Size: 643 5283
MfeI C'AATT_G
Cuts at: 2718 3068 3525 2718
Size: 350 457 5119
Fragments arranged by size:
5119 457 350
MluI A'CGCG_T
Cuts at: 5802 5802
Size: 5926
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2040 5040 5224 1305
Size: 735 3000 184 2007
Fragments arranged by size:
3000 2007 735 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2092 10
Size: 54 1425 343 260 3844
Fragments arranged by size:
3844 1425 343 260 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2588 2876 3884 4243 4402 518
Size: 648 1422 288 1008 359 159 2042
Fragments arranged by size:
2042 1422 1008 648 359 288 159
MspA1I CmG'CkG
Cuts at: 1606 3342 4019 5167 5412 1606
Size: 1736 677 1148 245 2120
Fragments arranged by size:
2120 1736 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5374
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5926
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5926
NotI GC'GGCC_GC
Cuts at: 3373 3373
Size: 5926
NsiI A_TGCA'T
Cuts at: 2383 2383
Size: 5926
NspI r_CATG'y
Cuts at: 1132 1229 2617 2699 3284 4829 1132
Size: 97 1388 82 585 1545 2229
Fragments arranged by size:
2229 1545 1388 585 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5926
PciI A'CATG_T
Cuts at: 4825 4825
Size: 5926
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5926
PmeI GTTT'AAAC
Cuts at: 3230 3230
Size: 5926
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3995 4027 5508 5540 3995
Size: 32 1481 32 4381
Fragments arranged by size:
4381 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5926
PsiI TTA'TAA
Cuts at: 3496 3496
Size: 5926
PstI C_TGCA'G
Cuts at: 830 2328 3278 830
Size: 1498 950 3478
Fragments arranged by size:
3478 1498 950
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5926
SacI G_AGCT'C
Cuts at: 2892 3118 2892
Size: 226 5700
SalI G'TCGA_C
Cuts at: 1936 3268 1936
Size: 1332 4594
SapI GCTCTTCn'nnn_
Cuts at: 2871 3106 2871
Size: 235 5691
SbfI CC_TGCA'GG
Cuts at: 3278 3278
Size: 5926
ScaI AGT'ACT
Cuts at: 4174 5886 4174
Size: 1712 4214
SexAI A'CCwGG_T
Cuts at: 2938 2938
Size: 5926
SfcI C'TryA_G
Cuts at: 826 1045 2109 2324 2825 3274 4409 5090 5281
Size: 219 1064 215 501 449 1135 681 191
Cuts at: 5281 826
Size: 1471
Fragments arranged by size:
1471 1135 1064 681 501 449 219 215
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5926
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5926
SmaI CCC'GGG
Cuts at: 1872 2141 3253 1872
Size: 269 1112 4545
Fragments arranged by size:
4545 1112 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2160 2184 4030 4931 5193 820
Size: 197 926 217 24 1846 901 262 1553
Fragments arranged by size:
1846 1553 926 901 262 217 197 24
SnaBI TAC'GTA
Cuts at: 493 3717 493
Size: 3224 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5926
SphI G_CATG'C
Cuts at: 1132 2699 3284 1132
Size: 1567 585 3774
Fragments arranged by size:
3774 1567 585
SspI AAT'ATT
Cuts at: 5 52 3850 5
Size: 47 3798 2081
Fragments arranged by size:
3798 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5926
StyI C'CwwG_G
Cuts at: 513 1065 2355 3365 513
Size: 552 1290 1010 3074
Fragments arranged by size:
3074 1290 1010 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2505 3953 3970 4123 4308 4753 325
Size: 1255 925 1448 17 153 185 445 1498
Fragments arranged by size:
1498 1448 1255 925 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3172 4172 5884
Size: 274 80 33 51 1443 1195 1000 1712
Cuts at: 5884 96
Size: 138
Fragments arranged by size:
1712 1443 1195 1000 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2501 2533 5886 5918 2501
Size: 32 3353 32 2509
Fragments arranged by size:
3353 2509 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5926
XbaI T'CTAG_A
Cuts at: 2281 3262 2281
Size: 981 4945
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5926
XmaI C'CCGG_G
Cuts at: 1870 2139 3251 1870
Size: 269 1112 4545
Fragments arranged by size:
4545 1112 269
XmnI GAAnn'nnTTC
Cuts at: 2244 4055 2244
Size: 1811 4115
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3734 276
Size: 53 83 186 936 2200 2468
Fragments arranged by size:
2468 2200 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI
XcmI