Restriction map for
pFN21AE3173
(Circular) (Six-base) MAPSORT of: pFN21AE3173.seq Check: 356 from: 1 to: 5923
pFN21AE3173 5923 bp
With 182 enzymes: SgfI *
March 28, 2012 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3018 3733 278
Size: 53 83 186 936 1482 715 2468
Fragments arranged by size:
2468 1482 936 715 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3266 1937
Size: 1329 4594
Acc65I G'GTAC_C
Cuts at: 3244 3244
Size: 5923
AclI AA'CG_TT
Cuts at: 1563 4050 4423 1563
Size: 2487 373 3063
Fragments arranged by size:
3063 2487 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2501 2831 3986 5370 5638 1632
Size: 869 330 1155 1384 268 1917
Fragments arranged by size:
1917 1384 1155 869 330 268
AfeI AGC'GCT
Cuts at: 3191 3191
Size: 5923
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5726
AflIII A'CryG_T
Cuts at: 1203 4822 5799 1203
Size: 3619 977 1327
Fragments arranged by size:
3619 1327 977
AgeI A'CCGG_T
Cuts at: 4754 4754
Size: 5923
AhdI GACnn_n'nnGTC
Cuts at: 4652 4652
Size: 5923
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5923
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5923
AlwNI CAG_nnn'CTG
Cuts at: 3666 5238 3666
Size: 1572 4351
ApaLI G'TGCA_C
Cuts at: 3980 5136 3980
Size: 1156 4767
ApoI r'AATT_y
Cuts at: 1493 2344 2841 3232 3458 3610 5719 1493
Size: 851 497 391 226 152 2109 1697
Fragments arranged by size:
2109 1697 851 497 391 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2721 4477 160
Size: 872 1689 1756 1606
Fragments arranged by size:
1756 1689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
AvaI C'yCGr_G
Cuts at: 1870 1943 2954 2990 3248 1870
Size: 73 1011 36 258 4545
Fragments arranged by size:
4545 1011 258 73 36
AvrII C'CTAG_G
Cuts at: 2023 3047 2023
Size: 1024 4899
BamHI G'GATC_C
Cuts at: 3253 3620 3253
Size: 367 5556
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2921 3244 4699 618
Size: 325 217 627 1134 323 1455 1842
Fragments arranged by size:
1842 1455 1134 627 325 323 217
BanII G_rGCy'C
Cuts at: 1391 2401 2648 3242 1391
Size: 1010 247 594 4072
Fragments arranged by size:
4072 1010 594 247
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2287 2460 2758 928
Size: 971 388 173 298 4093
Fragments arranged by size:
4093 971 388 298 173
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4114 4148 1903
Size: 34 2177 34 3678
Fragments arranged by size:
3678 2177 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3815 5025 3815
Size: 1210 4713
BclI T'GATC_A
Cuts at: 1548 2329 1548
Size: 781 5142
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4534 136
Size: 107 122 71 631 605 2862 1525
Fragments arranged by size:
2862 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3156 5918 3156
Size: 2762 3161
BlpI GC'TnA_GC
Cuts at: 2117 3340 2117
Size: 1223 4700
Bme1580I G_kGCm'C
Cuts at: 1790 3984 5140 1790
Size: 2194 1156 2573
Fragments arranged by size:
2573 2194 1156
BmgBI CAC'GTC
Cuts at: 1318 2458 1318
Size: 1140 4783
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2527 3654 4612
Size: 416 53 83 399 24 1110 1127 958
Cuts at: 4612 442
Size: 1753
Fragments arranged by size:
1753 1127 1110 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5923
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4583 1239
Size: 3344 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4048 4913 5211 4048
Size: 865 298 4760
Fragments arranged by size:
4760 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4586 882
Size: 617 3087 2219
Fragments arranged by size:
3087 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3714 493
Size: 711 2510 2702
Fragments arranged by size:
2702 2510 711
BsaBI GATnn'nnATC
Cuts at: 5917 5917
Size: 5923
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3015 3730 4112 4747
Size: 53 83 186 936 1482 715 382 635
Cuts at: 4747 275
Size: 1451
Fragments arranged by size:
1482 1451 936 715 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4356 4754 5028 5175 5586 4356
Size: 398 274 147 411 4693
Fragments arranged by size:
4693 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5698
Fragments arranged by size:
5698 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2900 2915 2918 1186
Size: 1714 15 3 4191
Fragments arranged by size:
4191 1714 15 3
BseYI C'CCAG_C
Cuts at: 2805 5126 5622 2805
Size: 2321 496 3106
Fragments arranged by size:
3106 2321 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5350
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3373 4134 5162 1540
Size: 275 186 1372 761 1028 2301
Fragments arranged by size:
2301 1372 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2401 3984 4069 5140 2401
Size: 1583 85 1071 3184
Fragments arranged by size:
3184 1583 1071 85
BsmI GAATG_Cn'
Cuts at: 3434 3527 3434
Size: 93 5830
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2401 2648 3242 3984 4069 5140 1391
Size: 399 611 247 594 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 611 594 399 247 85
BspEI T'CCGG_A
Cuts at: 5586 5586
Size: 5923
BspHI T'CATG_A
Cuts at: 2845 3812 5542 2845
Size: 967 1730 3226
Fragments arranged by size:
3226 1730 967
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2313 3278 844
Size: 1469 965 3489
Fragments arranged by size:
3489 1469 965
BsrBI CCG'CTC
Cuts at: 1394 1823 3810 1394
Size: 429 1987 3507
Fragments arranged by size:
3507 1987 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4418 4592 65
Size: 1183 3170 174 1396
Fragments arranged by size:
3170 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2550 4567 4754 1700
Size: 110 740 2017 187 2869
Fragments arranged by size:
2869 2017 740 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5923
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5923
BssSI C'ACGA_G
Cuts at: 3983 4995 3983
Size: 1012 4911
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5923
BstBI TT'CG_AA
Cuts at: 4727 4727
Size: 5923
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5923
BstYI r'GATC_y
Cuts at: 1972 3156 3253 3620 4005 4022 5474 5560 5572
Size: 1184 97 367 385 17 1452 86 12
Cuts at: 5572 5918 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1184 385 367 346 97 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4432
Fragments arranged by size:
4432 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2931 487
Size: 683 56 1705 3479
Fragments arranged by size:
3479 1705 683 56
BtsI GCAGTG_nn'
Cuts at: 2398 3439 4233 4253 2398
Size: 1041 794 20 4068
Fragments arranged by size:
4068 1041 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5923
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5888
DraI TTT'AAA
Cuts at: 3227 3227
Size: 5923
DraIII CAC_nnn'GTG
Cuts at: 2932 2932
Size: 5923
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4930 809
Size: 507 3614 1802
Fragments arranged by size:
3614 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2460 3208 3370
Size: 54 1425 50 275 18 630 748 162
Cuts at: 3370 4259 5888 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 748 630 275 162 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3370 1537
Size: 275 1558 4090
Fragments arranged by size:
4090 1558 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3853 1353
Size: 2500 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4510 4884 5030 387
Size: 1064 138 2921 374 146 1280
Fragments arranged by size:
2921 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2399 2399
Size: 5923
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2501 2831 3986 4583 5370 5638 1239
Size: 393 869 330 1155 597 787 268 1524
Fragments arranged by size:
1524 1155 869 787 597 393 330 268
EcoO109I rG'GnC_Cy
Cuts at: 2028 2028
Size: 5923
EcoRI G'AATT_C
Cuts at: 2344 2841 3232 3610 2344
Size: 497 391 378 4657
Fragments arranged by size:
4657 497 391 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2086 2782 2086
Size: 696 5227
FspI TGC'GCA
Cuts at: 4429 4429
Size: 5923
HaeII r_GCGC'y
Cuts at: 1678 3147 3193 5070 1678
Size: 1469 46 1877 2531
Fragments arranged by size:
2531 1877 1469 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2418 2450 4564 4596 4638
Size: 32 135 32 846 32 2114 32 42
Cuts at: 4638 4670 1373
Size: 32 2626
Fragments arranged by size:
2626 2114 846 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3267 3513 669
Size: 988 281 1329 246 3079
Fragments arranged by size:
3079 1329 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5923
HpaI GTT'AAC
Cuts at: 3513 3513
Size: 5923
KpnI G_GTAC'C
Cuts at: 3248 3248
Size: 5923
MfeI C'AATT_G
Cuts at: 3522 3522
Size: 5923
MluI A'CGCG_T
Cuts at: 5799 5799
Size: 5923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5037 5221 1305
Size: 3732 184 2007
Fragments arranged by size:
3732 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3210 10
Size: 54 1425 343 1378 2723
Fragments arranged by size:
2723 1425 1378 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3096 3881 4240 4399 518
Size: 648 1930 785 359 159 2042
Fragments arranged by size:
2042 1930 785 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2809 3339 4016 5164 5409 1606
Size: 507 696 530 677 1148 245 2120
Fragments arranged by size:
2120 1148 696 677 530 507 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4432
Fragments arranged by size:
4432 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5923
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5923
NotI GC'GGCC_GC
Cuts at: 3370 3370
Size: 5923
NspI r_CATG'y
Cuts at: 1132 1229 2257 2548 3281 4826 1132
Size: 97 1028 291 733 1545 2229
Fragments arranged by size:
2229 1545 1028 733 291 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5923
PciI A'CATG_T
Cuts at: 4822 4822
Size: 5923
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2539 2932 1108
Size: 1431 393 4099
Fragments arranged by size:
4099 1431 393
PfoI T'CCnGG_A
Cuts at: 2769 2769
Size: 5923
PmeI GTTT'AAAC
Cuts at: 3227 3227
Size: 5923
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3992 4024 5505 5537 3992
Size: 32 1481 32 4378
Fragments arranged by size:
4378 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2028 2028
Size: 5923
PshAI GACnn'nnGTC
Cuts at: 1613 2826 1613
Size: 1213 4710
PsiI TTA'TAA
Cuts at: 3493 3493
Size: 5923
PstI C_TGCA'G
Cuts at: 830 3275 830
Size: 2445 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5923
PvuII CAG'CTG
Cuts at: 2809 2809
Size: 5923
SacI G_AGCT'C
Cuts at: 2401 2401
Size: 5923
SalI G'TCGA_C
Cuts at: 1936 3265 1936
Size: 1329 4594
SbfI CC_TGCA'GG
Cuts at: 3275 3275
Size: 5923
ScaI AGT'ACT
Cuts at: 4171 5883 4171
Size: 1712 4211
SfcI C'TryA_G
Cuts at: 826 1045 2649 2824 3271 4406 5087 5278 826
Size: 219 1604 175 447 1135 681 191 1471
Fragments arranged by size:
1604 1471 1135 681 447 219 191 175
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5923
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5923
SmaI CCC'GGG
Cuts at: 1872 2956 2992 3250 1872
Size: 1084 36 258 4545
Fragments arranged by size:
4545 1084 258 36
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4027 4928 5190 820
Size: 197 926 2084 901 262 1553
Fragments arranged by size:
2084 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3714 493
Size: 3221 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5923
SphI G_CATG'C
Cuts at: 1132 3281 1132
Size: 2149 3774
SspI AAT'ATT
Cuts at: 5 52 3847 5
Size: 47 3795 2081
Fragments arranged by size:
3795 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5923
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 3047 3362 513
Size: 552 939 19 1024 315 3074
Fragments arranged by size:
3074 1024 939 552 315 19
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2939 3950 3967 4120 4305 4750 325
Size: 1255 1359 1011 17 153 185 445 1498
Fragments arranged by size:
1498 1359 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2496 3060 4169
Size: 274 80 33 51 1443 519 564 1109
Cuts at: 4169 5881 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 564 519 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2512 2544 2864 2896 5883 5915 2512
Size: 32 320 32 2987 32 2520
Fragments arranged by size:
2987 2520 320 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5923
XbaI T'CTAG_A
Cuts at: 3259 3259
Size: 5923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2390 2455 2390
Size: 65 5858
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5923
XmaI C'CCGG_G
Cuts at: 1870 2954 2990 3248 1870
Size: 1084 36 258 4545
Fragments arranged by size:
4545 1084 258 36
XmnI GAAnn'nnTTC
Cuts at: 2069 2765 4052 2069
Size: 696 1287 3940
Fragments arranged by size:
3940 1287 696
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3016 3731 276
Size: 53 83 186 936 1482 715 2468
Fragments arranged by size:
2468 1482 936 715 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI