Restriction map for
pFN21AE3172
(Circular) (Six-base) MAPSORT of: pFN21AE3172.seq Check: 568 from: 1 to: 5914
pFN21AE3172 5914 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3724 278
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3257 1937
Size: 1320 4594
Acc65I G'GTAC_C
Cuts at: 3235 3235
Size: 5914
AclI AA'CG_TT
Cuts at: 1563 4041 4414 1563
Size: 2478 373 3063
Fragments arranged by size:
3063 2478 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2029 2199 2364 3977 5361 5629 1632
Size: 397 170 165 1613 1384 268 1917
Fragments arranged by size:
1917 1613 1384 397 268 170 165
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5717
AflIII A'CryG_T
Cuts at: 1203 2121 4813 5790 1203
Size: 918 2692 977 1327
Fragments arranged by size:
2692 1327 977 918
AgeI A'CCGG_T
Cuts at: 4745 4745
Size: 5914
AhdI GACnn_n'nnGTC
Cuts at: 4643 4643
Size: 5914
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5914
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3022 1184
Size: 1838 4076
AlwNI CAG_nnn'CTG
Cuts at: 3657 5229 3657
Size: 1572 4342
ApaLI G'TGCA_C
Cuts at: 3971 5127 3971
Size: 1156 4758
ApoI r'AATT_y
Cuts at: 1493 3223 3449 3601 5710 1493
Size: 1730 226 152 2109 1697
Fragments arranged by size:
2109 1730 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4468 160
Size: 872 3436 1606
Fragments arranged by size:
3436 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
AvaI C'yCGr_G
Cuts at: 1870 1943 3239 1870
Size: 73 1296 4545
Fragments arranged by size:
4545 1296 73
BamHI G'GATC_C
Cuts at: 3244 3611 3244
Size: 367 5547
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2068 2588 3235 4690 618
Size: 325 217 627 281 520 647 1455 1842
Fragments arranged by size:
1842 1455 647 627 520 325 281 217
BanII G_rGCy'C
Cuts at: 1391 2355 3233 1391
Size: 964 878 4072
Fragments arranged by size:
4072 964 878
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2118 928
Size: 971 219 4724
Fragments arranged by size:
4724 971 219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4105 4139 1903
Size: 34 2168 34 3678
Fragments arranged by size:
3678 2168 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3806 5016 3806
Size: 1210 4704
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5914
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4525 136
Size: 107 122 71 631 605 2853 1525
Fragments arranged by size:
2853 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5909 5909
Size: 5914
BlpI GC'TnA_GC
Cuts at: 3331 3331
Size: 5914
Bme1580I G_kGCm'C
Cuts at: 1790 2593 3975 5131 1790
Size: 803 1382 1156 2573
Fragments arranged by size:
2573 1382 1156 803
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5914
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3645 4603 442
Size: 416 53 83 399 24 2228 958 1753
Fragments arranged by size:
2228 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5914
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3179 4574 1239
Size: 1940 1395 2579
Fragments arranged by size:
2579 1940 1395
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2236 3162 4039 4904 5202 2236
Size: 926 877 865 298 2948
Fragments arranged by size:
2948 926 877 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4577 882
Size: 617 3078 2219
Fragments arranged by size:
3078 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3077 3705 493
Size: 711 1873 628 2702
Fragments arranged by size:
2702 1873 711 628
BsaBI GATnn'nnATC
Cuts at: 2268 5908 2268
Size: 3640 2274
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3721 4103 4738 275
Size: 53 83 186 936 2188 382 635 1451
Fragments arranged by size:
2188 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4347 4745 5019 5166 5577 4347
Size: 398 274 147 411 4684
Fragments arranged by size:
4684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5689
Fragments arranged by size:
5689 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5914
BseYI C'CCAG_C
Cuts at: 2679 5117 5613 2679
Size: 2438 496 2980
Fragments arranged by size:
2980 2438 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5341
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3364 4125 5153 1540
Size: 275 186 1363 761 1028 2301
Fragments arranged by size:
2301 1363 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2355 3975 4060 5131 2355
Size: 1620 85 1071 3138
Fragments arranged by size:
3138 1620 1071 85
BsmI GAATG_Cn'
Cuts at: 3011 3172 3425 3518 3011
Size: 161 253 93 5407
Fragments arranged by size:
5407 253 161 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2355 2593 3233 3975 4060 5131 1391
Size: 399 565 238 640 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 640 565 399 238 85
BspEI T'CCGG_A
Cuts at: 5577 5577
Size: 5914
BspHI T'CATG_A
Cuts at: 3803 5533 3803
Size: 1730 4184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3269 844
Size: 2425 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3801 1394
Size: 429 1978 3507
Fragments arranged by size:
3507 1978 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2580 4409 4583 65
Size: 1183 1332 1829 174 1396
Fragments arranged by size:
1829 1396 1332 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4558 4745 1700
Size: 110 2748 187 2869
Fragments arranged by size:
2869 2748 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5914
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5914
BssSI C'ACGA_G
Cuts at: 3974 4986 3974
Size: 1012 4902
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5914
BstBI TT'CG_AA
Cuts at: 4718 4718
Size: 5914
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5914
BstXI CCAn_nnnn'nTGG
Cuts at: 2065 2065
Size: 5914
BstYI r'GATC_y
Cuts at: 1972 2269 3244 3611 3996 4013 5465 5551 5563
Size: 297 975 367 385 17 1452 86 12
Cuts at: 5563 5909 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 975 385 367 346 297 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5175
Fragments arranged by size:
5175 683 56
BtsI GCAGTG_nn'
Cuts at: 3430 4224 4244 3430
Size: 794 20 5100
Fragments arranged by size:
5100 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5914
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5879
DraI TTT'AAA
Cuts at: 2403 3218 2403
Size: 815 5099
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4921 809
Size: 507 3605 1802
Fragments arranged by size:
3605 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3361 4250 5879
Size: 54 1425 50 275 18 1531 889 1629
Cuts at: 5879 8
Size: 43
Fragments arranged by size:
1629 1531 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3361 1537
Size: 275 1549 4090
Fragments arranged by size:
4090 1549 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2084 2175 2343 3844 1353
Size: 731 91 168 1501 3423
Fragments arranged by size:
3423 1501 731 168 91
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4501 4875 5021 387
Size: 1064 138 2912 374 146 1280
Fragments arranged by size:
2912 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2353 2353
Size: 5914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2029 2199 2364 3179 3977 4574 5361
Size: 393 397 170 165 815 798 597 787
Cuts at: 5361 5629 1239
Size: 268 1524
Fragments arranged by size:
1524 815 798 787 597 397 393 268
170 165
EcoNI CCTnn'n_nnAGG
Cuts at: 2975 2975
Size: 5914
EcoRI G'AATT_C
Cuts at: 3223 3601 3223
Size: 378 5536
FspI TGC'GCA
Cuts at: 4420 4420
Size: 5914
HaeII r_GCGC'y
Cuts at: 1678 5061 1678
Size: 3383 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4555 4587 4629 4661 1373
Size: 32 135 32 2983 32 42 32 2626
Fragments arranged by size:
2983 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3258 3504 669
Size: 988 281 1320 246 3079
Fragments arranged by size:
3079 1320 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5914
HpaI GTT'AAC
Cuts at: 3504 3504
Size: 5914
KpnI G_GTAC'C
Cuts at: 3239 3239
Size: 5914
MfeI C'AATT_G
Cuts at: 3513 3513
Size: 5914
MluI A'CGCG_T
Cuts at: 5790 5790
Size: 5914
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2172 5028 5212 1305
Size: 867 2856 184 2007
Fragments arranged by size:
2856 2007 867 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4092
Fragments arranged by size:
4092 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2300 2573 3872 4231 4390 518
Size: 648 1134 273 1299 359 159 2042
Fragments arranged by size:
2042 1299 1134 648 359 273 159
MspA1I CmG'CkG
Cuts at: 1606 2141 3330 4007 5155 5400 1606
Size: 535 1189 677 1148 245 2120
Fragments arranged by size:
2120 1189 1148 677 535 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4423
Fragments arranged by size:
4423 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5914
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5914
NotI GC'GGCC_GC
Cuts at: 3361 3361
Size: 5914
NspI r_CATG'y
Cuts at: 1132 1229 2125 3272 4817 1132
Size: 97 896 1147 1545 2229
Fragments arranged by size:
2229 1545 1147 896 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5914
PciI A'CATG_T
Cuts at: 2121 4813 2121
Size: 2692 3222
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5914
PmeI GTTT'AAAC
Cuts at: 3218 3218
Size: 5914
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3983 4015 5496 5528 3983
Size: 32 1481 32 4369
Fragments arranged by size:
4369 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5914
PsiI TTA'TAA
Cuts at: 3484 3484
Size: 5914
PstI C_TGCA'G
Cuts at: 830 2881 3266 830
Size: 2051 385 3478
Fragments arranged by size:
3478 2051 385
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5914
PvuII CAG'CTG
Cuts at: 2141 2141
Size: 5914
SacI G_AGCT'C
Cuts at: 2355 2355
Size: 5914
SalI G'TCGA_C
Cuts at: 1936 3256 1936
Size: 1320 4594
SapI GCTCTTCn'nnn_
Cuts at: 2175 2343 2175
Size: 168 5746
SbfI CC_TGCA'GG
Cuts at: 3266 3266
Size: 5914
ScaI AGT'ACT
Cuts at: 4162 5874 4162
Size: 1712 4202
SexAI A'CCwGG_T
Cuts at: 2057 2057
Size: 5914
SfcI C'TryA_G
Cuts at: 826 1045 2877 3262 4397 5078 5269 826
Size: 219 1832 385 1135 681 191 1471
Fragments arranged by size:
1832 1471 1135 681 385 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5914
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5914
SmaI CCC'GGG
Cuts at: 1872 3241 1872
Size: 1369 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2251 3177 4018 4919 5181 820
Size: 197 926 308 926 841 901 262 1553
Fragments arranged by size:
1553 926 926 901 841 308 262 197
SnaBI TAC'GTA
Cuts at: 493 3077 3705 493
Size: 2584 628 2702
Fragments arranged by size:
2702 2584 628
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5914
SphI G_CATG'C
Cuts at: 1132 3272 1132
Size: 2140 3774
SspI AAT'ATT
Cuts at: 5 52 2399 2408 3026 3838 5
Size: 47 2347 9 618 812 2081
Fragments arranged by size:
2347 2081 812 618 47 9
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5914
StyI C'CwwG_G
Cuts at: 513 1065 2004 2976 3353 513
Size: 552 939 972 377 3074
Fragments arranged by size:
3074 972 939 552 377
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3941 3958 4111 4296 4741 325
Size: 1255 2361 17 153 185 445 1498
Fragments arranged by size:
2361 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2165 4160 5872
Size: 274 80 33 51 1443 188 1995 1712
Cuts at: 5872 96
Size: 138
Fragments arranged by size:
1995 1712 1443 274 188 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5874 5906 5874
Size: 32 5882
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5914
XbaI T'CTAG_A
Cuts at: 3250 3250
Size: 5914
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5914
XmaI C'CCGG_G
Cuts at: 1870 3239 1870
Size: 1369 4545
XmnI GAAnn'nnTTC
Cuts at: 3009 4043 3009
Size: 1034 4880
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3722 276
Size: 53 83 186 936 2188 2468
Fragments arranged by size:
2468 2188 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI
SrfI SwaI XcmI