Restriction map for
pFN21AE3169
(Circular) (Six-base) MAPSORT of: pFN21AE3169.seq Check: 2593 from: 1 to: 5902
pFN21AE3169 5902 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2381 2381
Size: 5902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3245 1937
Size: 1308 4594
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3965 5349 5617 1632
Size: 2333 1384 268 1917
Fragments arranged by size:
2333 1917 1384 268
AfeI AGC'GCT
Cuts at: 2240 2462 2881 2240
Size: 222 419 5261
Fragments arranged by size:
5261 419 222
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 2466 4801 5778 1203
Size: 1263 2335 977 1327
Fragments arranged by size:
2335 1327 1263 977
AgeI A'CCGG_T
Cuts at: 4733 4733
Size: 5902
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2826 1184
Size: 1642 4260
AlwNI CAG_nnn'CTG
Cuts at: 2086 2396 2854 3645 5217 2086
Size: 310 458 791 1572 2771
Fragments arranged by size:
2771 1572 791 458 310
ApaI G_GGCC'C
Cuts at: 2313 2554 2800 2313
Size: 241 246 5415
Fragments arranged by size:
5415 246 241
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3211 3437 3589 5698 1493
Size: 1718 226 152 2109 1697
Fragments arranged by size:
2109 1718 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 3227 1870
Size: 73 1284 4545
Fragments arranged by size:
4545 1284 73
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2303 2380 2713 2837 3097
Size: 325 217 627 516 77 333 124 260
Cuts at: 3097 3223 4678 618
Size: 126 1455 1842
Fragments arranged by size:
1842 1455 627 516 333 325 260 217
126 124 77
BanII G_rGCy'C
Cuts at: 1391 2313 2412 2531 2554 2800 3015 3221 1391
Size: 922 99 119 23 246 215 206 4072
Fragments arranged by size:
4072 922 246 215 206 119 99 23
BbeI G_GCGC'C
Cuts at: 2307 2384 2307
Size: 77 5825
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2484 928
Size: 971 585 4346
Fragments arranged by size:
4346 971 585
BbvCI CC'TCA_GC
Cuts at: 2876 2876
Size: 5902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2120 2154 4093 4127 1903
Size: 34 183 34 1939 34 3678
Fragments arranged by size:
3678 1939 183 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3794 5004 3794
Size: 1210 4692
BclI T'GATC_A
Cuts at: 1548 3069 1548
Size: 1521 4381
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2015 2711 4513
Size: 107 122 71 631 605 343 696 1802
Cuts at: 4513 136
Size: 1525
Fragments arranged by size:
1802 1525 696 631 605 343 122 107
71
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 2313 2554 2800 3100 3963 5119 1790
Size: 523 241 246 300 863 1156 2573
Fragments arranged by size:
2573 1156 863 523 300 246 241
BmgBI CAC'GTC
Cuts at: 1318 2498 1318
Size: 1180 4722
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2903 3633 4591
Size: 416 53 83 399 24 1486 730 958
Cuts at: 4591 442
Size: 1753
Fragments arranged by size:
1753 1486 958 730 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2515 4562 1239
Size: 1276 2047 2579
Fragments arranged by size:
2579 2047 1276
Bpu10I CC'TnA_GC
Cuts at: 2876 2876
Size: 5902
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4027 4892 5190 4027
Size: 865 298 4739
Fragments arranged by size:
4739 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2339 4565 882
Size: 617 840 2226 2219
Fragments arranged by size:
2226 2219 840 617
BsaAI yAC'GTr
Cuts at: 493 1204 2467 3693 493
Size: 711 1263 1226 2702
Fragments arranged by size:
2702 1263 1226 711
BsaBI GATnn'nnATC
Cuts at: 2087 3193 5896 2087
Size: 1106 2703 2093
Fragments arranged by size:
2703 2093 1106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2304 2381 3709 4091
Size: 53 83 186 936 771 77 1328 382
Cuts at: 4091 4726 275
Size: 635 1451
Fragments arranged by size:
1451 1328 936 771 635 382 186 83
77 53
BsaWI w'CCGG_w
Cuts at: 3199 4335 4733 5007 5154 5565 3199
Size: 1136 398 274 147 411 3536
Fragments arranged by size:
3536 1136 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 2591 2621 3145
Size: 30 165 30 912 30 246 30 524
Cuts at: 3145 3175 1178
Size: 30 3905
Fragments arranged by size:
3905 912 524 246 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2357 2420 2733 3104 1186
Size: 1171 63 313 371 3984
Fragments arranged by size:
3984 1171 371 313 63
BseYI C'CCAG_C
Cuts at: 2396 2457 2671 2799 3112 5105 5601 2396
Size: 61 214 128 313 1993 496 2697
Fragments arranged by size:
2697 1993 496 313 214 128 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2252 2520 2548 1163
Size: 573 516 268 28 4517
Fragments arranged by size:
4517 573 516 268 28
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3187 3352 4113 5141 1540
Size: 275 186 1186 165 761 1028 2301
Fragments arranged by size:
2301 1186 1028 761 275 186 165
BsiHKAI G_wGCw'C
Cuts at: 3963 4048 5119 3963
Size: 85 1071 4746
Fragments arranged by size:
4746 1071 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2313 2412 2531 2554 2800 3015 3100
Size: 399 523 99 119 23 246 215 85
Cuts at: 3100 3221 3963 4048 5119 1391
Size: 121 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 523 399 246 215 121
119 99 85 85 23
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 3257 844
Size: 1537 876 3489
Fragments arranged by size:
3489 1537 876
BsrBI CCG'CTC
Cuts at: 1394 1823 2158 3121 3789 1394
Size: 429 335 963 668 3507
Fragments arranged by size:
3507 963 668 429 335
BsrDI GCAATG_nn'
Cuts at: 65 1248 3072 4397 4571 65
Size: 1183 1824 1325 174 1396
Fragments arranged by size:
1824 1396 1325 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2632 2834 2983 4546 4733 1700
Size: 110 822 202 149 1563 187 2869
Fragments arranged by size:
2869 1563 822 202 187 149 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 2101 2127 1925
Size: 176 26 5700
Fragments arranged by size:
5700 176 26
BssSI C'ACGA_G
Cuts at: 3962 4974 3962
Size: 1012 4890
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5902
BstBI TT'CG_AA
Cuts at: 2070 4706 2070
Size: 2636 3266
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2082 3232 3599 3984 4001 5453 5539 5551
Size: 110 1150 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1150 385 367 346 110 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2316 2334 2546 2588 3021 513
Size: 552 939 312 18 212 42 433 3394
Fragments arranged by size:
3394 939 552 433 312 212 42 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2164 487
Size: 683 56 938 4225
Fragments arranged by size:
4225 938 683 56
BtsI GCAGTG_nn'
Cuts at: 2681 3054 3418 4212 4232 2681
Size: 373 364 794 20 4351
Fragments arranged by size:
4351 794 373 364 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2832 2856 3184
Size: 54 1425 50 275 18 1002 24 328
Cuts at: 3184 3349 4238 5867 8
Size: 165 889 1629 43
Fragments arranged by size:
1629 1425 1002 889 328 275 165 54
50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3184 3349 1537
Size: 275 1372 165 4090
Fragments arranged by size:
4090 1372 275 165
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2334 2431 3832 1353
Size: 714 267 97 1401 3423
Fragments arranged by size:
3423 1401 714 267 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2417 4489 4863 5009 387
Size: 1064 138 828 2072 374 146 1280
Fragments arranged by size:
2072 1280 1064 828 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2515 3965 4562 5349 5617 1239
Size: 393 883 1450 597 787 268 1524
Fragments arranged by size:
1524 1450 883 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2846 2846
Size: 5902
EcoO109I rG'GnC_Cy
Cuts at: 2872 2872
Size: 5902
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2077 2077
Size: 5902
FspI TGC'GCA
Cuts at: 2051 4408 2051
Size: 2357 3545
HaeII r_GCGC'y
Cuts at: 1678 2242 2260 2307 2384 2451 2464 2883 5049
Size: 564 18 47 77 67 13 419 2166
Cuts at: 5049 1678
Size: 2531
Fragments arranged by size:
2531 2166 564 419 77 67 47 18
13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2591 2623 3002
Size: 32 135 32 743 32 244 32 379
Cuts at: 3002 3034 4543 4575 4617 4649 1373
Size: 32 1509 32 42 32 2626
Fragments arranged by size:
2626 1509 743 379 244 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2914 3246 3492 669
Size: 988 281 976 332 246 3079
Fragments arranged by size:
3079 988 976 332 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KasI G'GCGC_C
Cuts at: 2303 2380 2303
Size: 77 5825
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2454 5016 5200 1305
Size: 1149 2562 184 2007
Fragments arranged by size:
2562 2007 1149 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2858 10
Size: 54 1425 343 1026 3054
Fragments arranged by size:
3054 1425 1026 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2208 2318 2336 2641 3318 3995 5143 5388
Size: 602 110 18 305 677 677 1148 245
Cuts at: 5388 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 677 602 305 245 110
18
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 5902
NarI GG'CG_CC
Cuts at: 2304 2381 2304
Size: 77 5825
NcoI C'CATG_G
Cuts at: 513 1065 2004 2546 513
Size: 552 939 542 3869
Fragments arranged by size:
3869 939 552 542
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PfoI T'CCnGG_A
Cuts at: 2823 2823
Size: 5902
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PspOMI G'GGCC_C
Cuts at: 2309 2550 2796 2309
Size: 241 246 5415
Fragments arranged by size:
5415 246 241
PstI C_TGCA'G
Cuts at: 830 2631 3254 830
Size: 1801 623 3478
Fragments arranged by size:
3478 1801 623
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
PvuII CAG'CTG
Cuts at: 2208 2641 2208
Size: 433 5469
SacII CC_GC'GG
Cuts at: 2319 2337 2319
Size: 18 5884
SalI G'TCGA_C
Cuts at: 1936 3244 1936
Size: 1308 4594
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 2627 3250 4385 5066 5257 826
Size: 219 1582 623 1135 681 191 1471
Fragments arranged by size:
1582 1471 1135 681 623 219 191
SfoI GGC'GCC
Cuts at: 2305 2382 2305
Size: 77 5825
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5902
SmaI CCC'GGG
Cuts at: 1872 3229 1872
Size: 1357 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4006 4907 5169 820
Size: 197 926 2063 901 262 1553
Fragments arranged by size:
2063 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 2968 1517
Size: 1451 4451
StyI C'CwwG_G
Cuts at: 513 1065 2004 2265 2546 2595 2991 3080 3341
Size: 552 939 261 281 49 396 89 261
Cuts at: 3341 513
Size: 3074
Fragments arranged by size:
3074 939 552 396 281 261 261 89
49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2508 2770 3929 3946 4099 4284 4729
Size: 1255 928 262 1159 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1498 1255 1159 928 445 262 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4148 5860 96
Size: 274 80 33 51 1443 2171 1712 138
Fragments arranged by size:
2171 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5862 5894 5862
Size: 32 5870
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2827 2827
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 3227 1870
Size: 1357 4545
XmnI GAAnn'nnTTC
Cuts at: 2249 4031 2249
Size: 1782 4120
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacII SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SacI SanDI SapI SexAI SfiI SrfI SwaI