Restriction map for
pFN21AE3163
(Circular) (Six-base) MAPSORT of: pFN21AE3163.seq Check: 1243 from: 1 to: 5848
pFN21AE3163 5848 bp
With 182 enzymes: SgfI *
March 28, 2012 12:39 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3658 278
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3191 1937
Size: 1254 4594
Acc65I G'GTAC_C
Cuts at: 3123 3169 3123
Size: 46 5802
AclI AA'CG_TT
Cuts at: 1563 3975 4348 1563
Size: 2412 373 3063
Fragments arranged by size:
3063 2412 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2912 3911 5295 5563 1632
Size: 1280 999 1384 268 1917
Fragments arranged by size:
1917 1384 1280 999 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5651
AflIII A'CryG_T
Cuts at: 1203 4747 5724 1203
Size: 3544 977 1327
Fragments arranged by size:
3544 1327 977
AgeI A'CCGG_T
Cuts at: 4679 4679
Size: 5848
AhdI GACnn_n'nnGTC
Cuts at: 4577 4577
Size: 5848
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5848
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2712 1184
Size: 1528 4320
AlwNI CAG_nnn'CTG
Cuts at: 2991 3591 5163 2991
Size: 600 1572 3676
Fragments arranged by size:
3676 1572 600
ApaLI G'TGCA_C
Cuts at: 3905 5061 3905
Size: 1156 4692
ApoI r'AATT_y
Cuts at: 1493 2158 2398 2455 2800 2849 3061 3157 3383
Size: 665 240 57 345 49 212 96 226
Cuts at: 3383 3535 5644 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 665 345 240 226 212 152
96 57 49
AseI AT'TA_AT
Cuts at: 160 1032 4402 160
Size: 872 3370 1606
Fragments arranged by size:
3370 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
AvaI C'yCGr_G
Cuts at: 1870 1943 2035 3173 1870
Size: 73 92 1138 4545
Fragments arranged by size:
4545 1138 92 73
BamHI G'GATC_C
Cuts at: 2596 3178 3545 2596
Size: 582 367 4899
Fragments arranged by size:
4899 582 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3123 3169 4624 618
Size: 325 217 627 1336 46 1455 1842
Fragments arranged by size:
1842 1455 1336 627 325 217 46
BanII G_rGCy'C
Cuts at: 1391 3167 1391
Size: 1776 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2457 928
Size: 971 558 4319
Fragments arranged by size:
4319 971 558
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2572 2606 4039 4073 1903
Size: 34 635 34 1433 34 3678
Fragments arranged by size:
3678 1433 635 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2936 3740 4950 2936
Size: 804 1210 3834
Fragments arranged by size:
3834 1210 804
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5848
BfrBI ATG'CAT
Cuts at: 2625 2625
Size: 5848
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4459 136
Size: 107 122 71 631 605 2787 1525
Fragments arranged by size:
2787 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5843 5843
Size: 5848
BlpI GC'TnA_GC
Cuts at: 3265 3265
Size: 5848
Bme1580I G_kGCm'C
Cuts at: 1790 3909 5065 1790
Size: 2119 1156 2573
Fragments arranged by size:
2573 2119 1156
BmgBI CAC'GTC
Cuts at: 1318 2838 1318
Size: 1520 4328
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2447 3579 4537
Size: 416 53 83 399 24 1030 1132 958
Cuts at: 4537 442
Size: 1753
Fragments arranged by size:
1753 1132 1030 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5848
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 4508 1239
Size: 968 2301 2579
Fragments arranged by size:
2579 2301 968
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3973 4838 5136 3973
Size: 865 298 4685
Fragments arranged by size:
4685 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4511 882
Size: 617 3012 2219
Fragments arranged by size:
3012 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3094 3639 493
Size: 711 1890 545 2702
Fragments arranged by size:
2702 1890 711 545
BsaBI GATnn'nnATC
Cuts at: 5842 5842
Size: 5848
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3655 4037 4672 275
Size: 53 83 186 936 2122 382 635 1451
Fragments arranged by size:
2122 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4281 4679 4953 5100 5511 4281
Size: 398 274 147 411 4618
Fragments arranged by size:
4618 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2016 2046 2824 2854 3107
Size: 30 165 30 613 30 778 30 253
Cuts at: 3107 3137 1178
Size: 30 3889
Fragments arranged by size:
3889 778 613 253 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5848
BseYI C'CCAG_C
Cuts at: 2075 3047 3051 3144 5051 5547 2075
Size: 972 4 93 1907 496 2376
Fragments arranged by size:
2376 1907 972 496 93 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3298 4059 5087 1540
Size: 275 186 1297 761 1028 2301
Fragments arranged by size:
2301 1297 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3909 3994 5065 3909
Size: 85 1071 4692
Fragments arranged by size:
4692 1071 85
BsmI GAATG_Cn'
Cuts at: 3359 3452 3359
Size: 93 5755
BsmBI CGTCTCn'nnnn_
Cuts at: 2844 2844
Size: 5848
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3167 3909 3994 5065 1391
Size: 399 1377 742 85 1071 2174
Fragments arranged by size:
2174 1377 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5511 5511
Size: 5848
BspHI T'CATG_A
Cuts at: 2413 3737 5467 2413
Size: 1324 1730 2794
Fragments arranged by size:
2794 1730 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3203 844
Size: 2359 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3735 1394
Size: 429 1912 3507
Fragments arranged by size:
3507 1912 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4343 4517 65
Size: 1183 3095 174 1396
Fragments arranged by size:
3095 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4492 4679 1700
Size: 110 2682 187 2869
Fragments arranged by size:
2869 2682 187 110
BsrGI T'GTAC_A
Cuts at: 96 2250 96
Size: 2154 3694
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5848
BssSI C'ACGA_G
Cuts at: 2844 3908 4920 2844
Size: 1064 1012 3772
Fragments arranged by size:
3772 1064 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2028 794
Size: 1234 4614
BstBI TT'CG_AA
Cuts at: 4652 4652
Size: 5848
BstEII G'GTnAC_C
Cuts at: 1184 2555 1184
Size: 1371 4477
BstYI r'GATC_y
Cuts at: 1972 2167 2596 3178 3545 3930 3947 5399 5485
Size: 195 429 582 367 385 17 1452 86
Cuts at: 5485 5497 5843 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 582 429 385 367 346 195
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2532 2532
Size: 5848
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5296
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5109
Fragments arranged by size:
5109 683 56
BtsI GCAGTG_nn'
Cuts at: 3125 3364 4158 4178 3125
Size: 239 794 20 4795
Fragments arranged by size:
4795 794 239 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5848
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5813
DraI TTT'AAA
Cuts at: 3152 3152
Size: 5848
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4855 809
Size: 507 3539 1802
Fragments arranged by size:
3539 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3295 4184 5813
Size: 54 1425 50 275 18 1465 889 1629
Cuts at: 5813 8
Size: 43
Fragments arranged by size:
1629 1465 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3295 1537
Size: 275 1483 4090
Fragments arranged by size:
4090 1483 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2532 2706 3778 1353
Size: 1179 174 1072 3423
Fragments arranged by size:
3423 1179 1072 174
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4435 4809 4955 387
Size: 1064 138 2846 374 146 1280
Fragments arranged by size:
2846 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2912 3911 4508 5295 5563 1239
Size: 393 575 705 999 597 787 268 1524
Fragments arranged by size:
1524 999 787 705 597 575 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2405 2405
Size: 5848
EcoRI G'AATT_C
Cuts at: 2455 3061 3157 3535 2455
Size: 606 96 378 4768
Fragments arranged by size:
4768 606 378 96
FspI TGC'GCA
Cuts at: 4354 4354
Size: 5848
HaeII r_GCGC'y
Cuts at: 1678 4995 1678
Size: 3317 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 2549 2581 2824
Size: 32 135 32 427 32 518 32 243
Cuts at: 2824 2856 4489 4521 4563 4595 1373
Size: 32 1633 32 42 32 2626
Fragments arranged by size:
2626 1633 518 427 243 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3192 3438 669
Size: 988 281 1254 246 3079
Fragments arranged by size:
3079 1254 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5848
HpaI GTT'AAC
Cuts at: 3438 3438
Size: 5848
KpnI G_GTAC'C
Cuts at: 3127 3173 3127
Size: 46 5802
MfeI C'AATT_G
Cuts at: 2522 3004 3447 2522
Size: 482 443 4923
Fragments arranged by size:
4923 482 443
MluI A'CGCG_T
Cuts at: 5724 5724
Size: 5848
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4962 5146 1305
Size: 3657 184 2007
Fragments arranged by size:
3657 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4026
Fragments arranged by size:
4026 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2873 3806 4165 4324 518
Size: 648 1707 933 359 159 2042
Fragments arranged by size:
2042 1707 933 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2087 3051 3264 3941 5089 5334 1606
Size: 481 964 213 677 1148 245 2120
Fragments arranged by size:
2120 1148 964 677 481 245 213
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5296
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5848
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5848
NotI GC'GGCC_GC
Cuts at: 3295 3295
Size: 5848
NsiI A_TGCA'T
Cuts at: 2627 2627
Size: 5848
NspI r_CATG'y
Cuts at: 1132 1229 2720 3206 4751 1132
Size: 97 1491 486 1545 2229
Fragments arranged by size:
2229 1545 1491 486 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5848
PciI A'CATG_T
Cuts at: 4747 4747
Size: 5848
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5848
PfoI T'CCnGG_A
Cuts at: 2223 2928 2223
Size: 705 5143
PmeI GTTT'AAAC
Cuts at: 3152 3152
Size: 5848
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3917 3949 5430 5462 3917
Size: 32 1481 32 4303
Fragments arranged by size:
4303 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2405 2405
Size: 5848
PshAI GACnn'nnGTC
Cuts at: 1613 2421 1613
Size: 808 5040
PsiI TTA'TAA
Cuts at: 2331 3418 2331
Size: 1087 4761
PstI C_TGCA'G
Cuts at: 830 2450 3200 830
Size: 1620 750 3478
Fragments arranged by size:
3478 1620 750
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5848
PvuII CAG'CTG
Cuts at: 2087 3051 2087
Size: 964 4884
SalI G'TCGA_C
Cuts at: 1936 3190 1936
Size: 1254 4594
SapI GCTCTTCn'nnn_
Cuts at: 2532 2706 2532
Size: 174 5674
SbfI CC_TGCA'GG
Cuts at: 3200 3200
Size: 5848
ScaI AGT'ACT
Cuts at: 4096 5808 4096
Size: 1712 4136
SfcI C'TryA_G
Cuts at: 826 1045 2082 2446 3196 4331 5012 5203 826
Size: 219 1037 364 750 1135 681 191 1471
Fragments arranged by size:
1471 1135 1037 750 681 364 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5848
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5848
SmaI CCC'GGG
Cuts at: 1872 2037 3175 1872
Size: 165 1138 4545
Fragments arranged by size:
4545 1138 165
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3952 4853 5115 820
Size: 197 926 2009 901 262 1553
Fragments arranged by size:
2009 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3094 3639 493
Size: 2601 545 2702
Fragments arranged by size:
2702 2601 545
SpeI A'CTAG_T
Cuts at: 152 2651 152
Size: 2499 3349
SphI G_CATG'C
Cuts at: 1132 3206 1132
Size: 2074 3774
SspI AAT'ATT
Cuts at: 5 52 3772 5
Size: 47 3720 2081
Fragments arranged by size:
3720 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5848
StyI C'CwwG_G
Cuts at: 513 1065 3287 513
Size: 552 2222 3074
Fragments arranged by size:
3074 2222 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2623 3043 3127 3875 3892 4045 4230
Size: 1255 1043 420 84 748 17 153 185
Cuts at: 4230 4675 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1043 748 445 420 185 153
84 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 4094 5806
Size: 274 80 33 51 1443 273 1844 1712
Cuts at: 5806 96
Size: 138
Fragments arranged by size:
1844 1712 1443 274 273 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5808 5840 5808
Size: 32 5816
Tth111I GACn'n_nGTC
Cuts at: 1316 2836 1316
Size: 1520 4328
XbaI T'CTAG_A
Cuts at: 3184 3184
Size: 5848
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2954 2954
Size: 5848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5848
XmaI C'CCGG_G
Cuts at: 1870 2035 3173 1870
Size: 165 1138 4545
Fragments arranged by size:
4545 1138 165
XmnI GAAnn'nnTTC
Cuts at: 3977 3977
Size: 5848
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3656 276
Size: 53 83 186 936 2122 2468
Fragments arranged by size:
2468 2122 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BstXI BstZ17I DraIII EcoICRI
EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI