Restriction map for
pFN21AE3162
(Circular) (Six-base) MAPSORT of: pFN21AE3162.seq Check: 4493 from: 1 to: 5845
pFN21AE3162 5845 bp
With 182 enzymes: SgfI *
January 30, 2014 17:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3655 278
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3188 1937
Size: 1251 4594
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3144 3972 4345 1563
Size: 1581 828 373 3063
Fragments arranged by size:
3063 1581 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2151 2546 3908 5292 5560 1632
Size: 519 395 1362 1384 268 1917
Fragments arranged by size:
1917 1384 1362 519 395 268
AfeI AGC'GCT
Cuts at: 2531 2531
Size: 5845
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5648
AflIII A'CryG_T
Cuts at: 1203 2829 2958 3111 4744 5721 1203
Size: 1626 129 153 1633 977 1327
Fragments arranged by size:
1633 1626 1327 977 153 129
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5845
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2122 1184
Size: 938 4907
AlwNI CAG_nnn'CTG
Cuts at: 2716 3588 5160 2716
Size: 872 1572 3401
Fragments arranged by size:
3401 1572 872
ApaI G_GGCC'C
Cuts at: 2036 2074 2036
Size: 38 5807
ApaLI G'TGCA_C
Cuts at: 3902 5058 3902
Size: 1156 4689
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 2066 2637 3170 1870
Size: 73 123 571 533 4545
Fragments arranged by size:
4545 571 533 123 73
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2461 2633 2690 2932 3166
Size: 325 217 627 674 172 57 242 234
Cuts at: 3166 4621 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 674 627 325 242 234 217
172 57
BanII G_rGCy'C
Cuts at: 1391 2036 2074 2662 2704 3164 1391
Size: 645 38 588 42 460 4072
Fragments arranged by size:
4072 645 588 460 42 38
BbeI G_GCGC'C
Cuts at: 2465 2637 2694 2465
Size: 172 57 5616
Fragments arranged by size:
5616 172 57
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2353 928
Size: 971 454 4420
Fragments arranged by size:
4420 971 454
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2439 2688 4456
Size: 107 122 71 631 605 767 249 1768
Cuts at: 4456 136
Size: 1525
Fragments arranged by size:
1768 1525 767 631 605 249 122 107
71
BglII A'GATC_T
Cuts at: 2769 5840 2769
Size: 3071 2774
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2036 2074 2921 2937 3906 5062 1790
Size: 246 38 847 16 969 1156 2573
Fragments arranged by size:
2573 1156 969 847 246 38 16
BmgBI CAC'GTC
Cuts at: 1318 2020 2209 2832 1318
Size: 702 189 623 4331
Fragments arranged by size:
4331 702 623 189
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2807 3576 4534
Size: 416 53 83 399 24 1390 769 958
Cuts at: 4534 442
Size: 1753
Fragments arranged by size:
1753 1390 958 769 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 4505 1239
Size: 1097 2169 2579
Fragments arranged by size:
2579 2169 1097
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4508 882
Size: 617 3009 2219
Fragments arranged by size:
3009 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2392 2494 2944 2959 2994 3133 3636
Size: 711 1188 102 450 15 35 139 503
Cuts at: 3636 493
Size: 2702
Fragments arranged by size:
2702 1188 711 503 450 139 102 35
15
BsaBI GATnn'nnATC
Cuts at: 2951 5839 2951
Size: 2888 2957
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2062 2462 2634 2691
Size: 53 83 186 936 529 400 172 57
Cuts at: 2691 3652 4034 4669 275
Size: 961 382 635 1451
Fragments arranged by size:
1451 961 936 635 529 400 382 186
172 83 57 53
BsaWI w'CCGG_w
Cuts at: 4278 4676 4950 5097 5508 4278
Size: 398 274 147 411 4615
Fragments arranged by size:
4615 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 2309 2339 1178
Size: 30 165 30 702 30 174 30 4684
Fragments arranged by size:
4684 702 174 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2260 2363 1186
Size: 1074 103 4668
Fragments arranged by size:
4668 1074 103
BseYI C'CCAG_C
Cuts at: 2705 5048 5544 2705
Size: 2343 496 3006
Fragments arranged by size:
3006 2343 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2276 3146 1163
Size: 573 540 870 3862
Fragments arranged by size:
3862 870 573 540
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2655 3295 4056 5084 1540
Size: 275 186 654 640 761 1028 2301
Fragments arranged by size:
2301 1028 761 654 640 275 186
BsiHKAI G_wGCw'C
Cuts at: 3906 3991 5062 3906
Size: 85 1071 4689
Fragments arranged by size:
4689 1071 85
BsmI GAATG_Cn'
Cuts at: 2754 3356 3449 2754
Size: 602 93 5150
Fragments arranged by size:
5150 602 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2074 2662 2704 2921 2937 3164
Size: 399 246 38 588 42 217 16 227
Cuts at: 3164 3906 3991 5062 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 588 399 246 227 217
85 42 38 16
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2259 2337 3200 844
Size: 1415 78 863 3489
Fragments arranged by size:
3489 1415 863 78
BsrBI CCG'CTC
Cuts at: 1394 1823 3031 3732 1394
Size: 429 1208 701 3507
Fragments arranged by size:
3507 1208 701 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4340 4514 65
Size: 1183 3092 174 1396
Fragments arranged by size:
3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2102 2137 4489 4676 1700
Size: 110 292 35 2352 187 2869
Fragments arranged by size:
2869 2352 292 187 110 35
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5845
BssHII G'CGCG_C
Cuts at: 1925 2613 1925
Size: 688 5157
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2494 794
Size: 1700 4145
BstBI TT'CG_AA
Cuts at: 4649 4649
Size: 5845
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 2769 3175 3542 3927 3944 5396 5482 5494
Size: 797 406 367 385 17 1452 86 12
Cuts at: 5494 5840 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 797 406 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2677 2908 513
Size: 552 939 673 231 3450
Fragments arranged by size:
3450 939 673 552 231
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5106
Fragments arranged by size:
5106 683 56
BtsI GCAGTG_nn'
Cuts at: 2124 2509 3361 4155 4175 2124
Size: 385 852 794 20 3794
Fragments arranged by size:
3794 852 794 385 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2652 2824 3292
Size: 54 1425 50 275 18 822 172 468
Cuts at: 3292 4181 5810 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 822 468 275 172 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2652 3292 1537
Size: 275 840 640 4090
Fragments arranged by size:
4090 840 640 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3775 1353
Size: 2422 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4432 4806 4952 387
Size: 1064 138 2843 374 146 1280
Fragments arranged by size:
2843 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2151 2336 2546 3908 4505 5292 5560
Size: 393 519 185 210 1362 597 787 268
Cuts at: 5560 1239
Size: 1524
Fragments arranged by size:
1524 1362 787 597 519 393 268 210
185
EcoNI CCTnn'n_nnAGG
Cuts at: 2318 2318
Size: 5845
EcoO109I rG'GnC_Cy
Cuts at: 2032 2033 2070 2071 2641 3097 2032
Size: 1 37 1 570 456 4780
Fragments arranged by size:
4780 570 456 37 1 1
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2905 2905
Size: 5845
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 2138 2151 2233 2344 2423 2465 2474 2486
Size: 460 13 82 111 79 42 9 12
Cuts at: 2486 2504 2533 2637 2694 4992 1678
Size: 18 29 104 57 2298 2531
Fragments arranged by size:
2531 2298 460 111 104 82 79 57
42 29 18 13 12 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2105 2137 4486 4518 4560
Size: 32 135 32 533 32 2349 32 42
Cuts at: 4560 4592 1373
Size: 32 2626
Fragments arranged by size:
2626 2349 533 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KasI G'GCGC_C
Cuts at: 2461 2633 2690 2461
Size: 172 57 5616
Fragments arranged by size:
5616 172 57
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3132 4959 5143 1305
Size: 1827 1827 184 2007
Fragments arranged by size:
2007 1827 1827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4023
Fragments arranged by size:
4023 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2942 2957 3056 3803 4162 4321 518
Size: 648 1776 15 99 747 359 159 2042
Fragments arranged by size:
2042 1776 747 648 359 159 99 15
MspA1I CmG'CkG
Cuts at: 1606 2119 2283 2338 2605 2823 2862 2910 3261
Size: 513 164 55 267 218 39 48 351
Cuts at: 3261 3938 5086 5331 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 513 351 267 245 218
164 55 48 39
NaeI GCC'GGC
Cuts at: 2104 2139 2104
Size: 35 5810
NarI GG'CG_CC
Cuts at: 2462 2634 2691 2462
Size: 172 57 5616
Fragments arranged by size:
5616 172 57
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4354
Fragments arranged by size:
4354 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2102 2137 2102
Size: 35 5810
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 3203 4748 1132
Size: 97 1974 1545 2229
Fragments arranged by size:
2229 1974 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2685 1348
Size: 1337 4508
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PmlI CAC'GTG
Cuts at: 2392 2994 2392
Size: 602 5243
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2641 3097 2641
Size: 456 5389
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5845
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PspOMI G'GGCC_C
Cuts at: 2032 2070 2032
Size: 38 5807
PstI C_TGCA'G
Cuts at: 830 2334 2403 2490 2867 3197 830
Size: 1504 69 87 377 330 3478
Fragments arranged by size:
3478 1504 377 330 87 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
PvuII CAG'CTG
Cuts at: 2862 2862
Size: 5845
SacII CC_GC'GG
Cuts at: 2911 2911
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SanDI GG'GwC_CC
Cuts at: 2641 2641
Size: 5845
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SfcI C'TryA_G
Cuts at: 826 1045 2330 2399 2486 2863 2902 3193 4328
Size: 219 1285 69 87 377 39 291 1135
Cuts at: 4328 5009 5200 826
Size: 681 191 1471
Fragments arranged by size:
1471 1285 1135 681 377 291 219 191
87 69 39
SfoI GGC'GCC
Cuts at: 2463 2635 2692 2463
Size: 172 57 5616
Fragments arranged by size:
5616 172 57
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 2068 2639 3172 1872
Size: 196 571 533 4545
Fragments arranged by size:
4545 571 533 196
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3949 4850 5112 820
Size: 197 926 2006 901 262 1553
Fragments arranged by size:
2006 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3133 3636 493
Size: 2640 503 2702
Fragments arranged by size:
2702 2640 503
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5845
StyI C'CwwG_G
Cuts at: 513 1065 2004 3284 513
Size: 552 939 1280 3074
Fragments arranged by size:
3074 1280 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2318 3872 3889 4042 4227 4672 325
Size: 1255 738 1554 17 153 185 445 1498
Fragments arranged by size:
1554 1498 1255 738 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4091 5803 96
Size: 274 80 33 51 1443 2114 1712 138
Fragments arranged by size:
2114 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 2066 2637 3170 1870
Size: 196 571 533 4545
Fragments arranged by size:
4545 571 533 196
XmnI GAAnn'nnTTC
Cuts at: 2531 3974 2531
Size: 1443 4402
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3653 276
Size: 53 83 186 936 2119 2468
Fragments arranged by size:
2468 2119 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI NruI NsiI PacI PfoI PspXI
PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI
XcmI