Restriction map for
pFN21AE3142
(Circular) (Six-base) MAPSORT of: pFN21AE3142.seq Check: 1880 from: 1 to: 5764
pFN21AE3142 5764 bp
With 182 enzymes: SgfI *
March 28, 2012 12:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2475 2475
Size: 5764
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2915 3107 1937
Size: 978 192 4594
Fragments arranged by size:
4594 978 192
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2681 3827 5211 5479 1632
Size: 1049 1146 1384 268 1917
Fragments arranged by size:
1917 1384 1146 1049 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 4663 5640 1203
Size: 3460 977 1327
Fragments arranged by size:
3460 1327 977
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 2951 1166
Size: 1785 3979
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2386 2418 2696 2728 2386
Size: 32 278 32 5422
Fragments arranged by size:
5422 278 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3049 3507 5079 3049
Size: 458 1572 3734
Fragments arranged by size:
3734 1572 458
ApaI G_GGCC'C
Cuts at: 2377 2533 2377
Size: 156 5608
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 3073 3299 3451 5560 1493
Size: 1580 226 152 2109 1697
Fragments arranged by size:
2109 1697 1580 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 3089 1870
Size: 73 1146 4545
Fragments arranged by size:
4545 1146 73
AvrII C'CTAG_G
Cuts at: 2809 2809
Size: 5764
BamHI G'GATC_C
Cuts at: 2434 3094 3461 2434
Size: 660 367 4737
Fragments arranged by size:
4737 660 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3085 4540 618
Size: 325 217 627 1298 1455 1842
Fragments arranged by size:
1842 1455 1298 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2377 2533 2589 2818 3083 1391
Size: 986 156 56 229 265 4072
Fragments arranged by size:
4072 986 265 229 156 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4793
BbvCI CC'TCA_GC
Cuts at: 2632 2632
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2732 2745 3656 4866 2732
Size: 13 911 1210 3630
Fragments arranged by size:
3630 1210 911 13
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5764
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 2156 2377 2533 3825 4981 1790
Size: 366 221 156 1292 1156 2573
Fragments arranged by size:
2573 1292 1156 366 221 156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2318 3495 4453
Size: 416 53 83 399 24 901 1177 958
Cuts at: 4453 442
Size: 1753
Fragments arranged by size:
1753 1177 958 901 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2300 2698 2852 4424 1239
Size: 1061 398 154 1572 2579
Fragments arranged by size:
2579 1572 1061 398 154
Bpu10I CC'TnA_GC
Cuts at: 2453 2632 2453
Size: 179 5585
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4754 5052 3889
Size: 865 298 4601
Fragments arranged by size:
4601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2231 4427 882
Size: 617 732 2196 2219
Fragments arranged by size:
2219 2196 732 617
BsaAI yAC'GTr
Cuts at: 493 1204 3555 493
Size: 711 2351 2702
Fragments arranged by size:
2702 2351 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3571 3953 4588 275
Size: 53 83 186 936 2038 382 635 1451
Fragments arranged by size:
2038 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2454 2484 2696 2726 2834
Size: 30 165 30 1051 30 212 30 108
Cuts at: 2834 2850 2864 2880 2884 2914 3023 3053 1178
Size: 16 14 16 4 30 109 30 3889
Fragments arranged by size:
3889 1051 212 165 109 108 30 30
30 30 30 30 16 16 14 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2015 2067 2249 2354 1186
Size: 829 52 182 105 4596
Fragments arranged by size:
4596 829 182 105 52
BseYI C'CCAG_C
Cuts at: 2397 2552 4967 5463 2397
Size: 155 2415 496 2698
Fragments arranged by size:
2698 2415 496 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2589 3825 3910 4981 2589
Size: 1236 85 1071 3372
Fragments arranged by size:
3372 1236 1071 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
BsmBI CGTCTCn'nnnn_
Cuts at: 2366 2366
Size: 5764
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2156 2377 2533 2589 2818 3083 3825
Size: 399 366 221 156 56 229 265 742
Cuts at: 3825 3910 4981 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 366 265 229 221
156 85 56
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 3653 5383 3653
Size: 1730 4034
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2475 3119 844
Size: 1631 644 3489
Fragments arranged by size:
3489 1631 644
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 2027 2775 3824 4836 2027
Size: 748 1049 1012 2955
Fragments arranged by size:
2955 1049 1012 748
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2722 2953 2722
Size: 231 5533
BstYI r'GATC_y
Cuts at: 1972 2434 3094 3461 3846 3863 5315 5401 5413
Size: 462 660 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 660 462 385 367 346 86
17 12
BstZ17I GTA'TAC
Cuts at: 2916 2916
Size: 5764
Bsu36I CC'TnA_GG
Cuts at: 2830 2830
Size: 5764
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5025
Fragments arranged by size:
5025 683 56
BtsI GCAGTG_nn'
Cuts at: 2271 2625 3280 4074 4094 2271
Size: 354 655 794 20 3941
Fragments arranged by size:
3941 794 655 354 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2922 2957 529
Size: 35 2358 35 3336
Fragments arranged by size:
3336 2358 35 35
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2096 2126 2358 4771 809
Size: 507 780 30 232 2413 1802
Fragments arranged by size:
2413 1802 780 507 232 30
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729
Size: 54 1425 50 275 18 1381 889 1629
Cuts at: 5729 8
Size: 43
Fragments arranged by size:
1629 1425 1381 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2927 2934 3021 3694 1353
Size: 1574 7 87 673 3423
Fragments arranged by size:
3423 1574 673 87 7
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2587 2587
Size: 5764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2300 2681 2698 2852 3827 4424 5211
Size: 393 668 381 17 154 975 597 787
Cuts at: 5211 5479 1239
Size: 268 1524
Fragments arranged by size:
1524 975 787 668 597 393 381 268
154 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2800 2800
Size: 5764
EcoO109I rG'GnC_Cy
Cuts at: 2312 2373 2374 2530 2718 2805 2312
Size: 61 1 156 188 87 5271
Fragments arranged by size:
5271 188 156 87 61 1
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 4911 1678
Size: 3233 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2410 2442 2680 2712 2813
Size: 32 135 32 838 32 238 32 101
Cuts at: 2813 2834 2845 2866 4405 4437 4479 4511 1373
Size: 21 11 21 1539 32 42 32 2626
Fragments arranged by size:
2626 1539 838 238 135 101 42 32
32 32 32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3108 3354 669
Size: 988 281 1170 246 3079
Fragments arranged by size:
3079 1170 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5764
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2485 2592 3017 4878 5062 1305
Size: 1180 107 425 1861 184 2007
Fragments arranged by size:
2007 1861 1180 425 184 107
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2951 3722 4081 4240 518
Size: 648 1785 771 359 159 2042
Fragments arranged by size:
2042 1785 771 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2546 3180 3857 5005 5250 1606
Size: 940 634 677 1148 245 2120
Fragments arranged by size:
2120 1148 940 677 634 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4273
Fragments arranged by size:
4273 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 3122 4667 1132
Size: 97 1893 1545 2229
Fragments arranged by size:
2229 1893 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 4663 4663
Size: 5764
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PfoI T'CCnGG_A
Cuts at: 2788 2788
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2696 2728 3833 3865 5346 5378 2696
Size: 32 1105 32 1481 32 3082
Fragments arranged by size:
3082 1481 1105 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2312 2718 2805 2312
Size: 406 87 5271
Fragments arranged by size:
5271 406 87
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 3334 3334
Size: 5764
PspOMI G'GGCC_C
Cuts at: 2373 2529 2373
Size: 156 5608
PstI C_TGCA'G
Cuts at: 830 2382 3116 830
Size: 1552 734 3478
Fragments arranged by size:
3478 1552 734
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
PvuII CAG'CTG
Cuts at: 2546 2546
Size: 5764
SacI G_AGCT'C
Cuts at: 2589 2589
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SanDI GG'GwC_CC
Cuts at: 2312 2312
Size: 5764
SapI GCTCTTCn'nnn_
Cuts at: 2927 2927
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SfcI C'TryA_G
Cuts at: 826 1045 2378 3112 4247 4928 5119 826
Size: 219 1333 734 1135 681 191 1471
Fragments arranged by size:
1471 1333 1135 734 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 3091 1872
Size: 1219 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3868 4769 5031 820
Size: 197 926 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 2004 2241 2368 2389 2809 3203 513
Size: 552 939 237 127 21 420 394 3074
Fragments arranged by size:
3074 939 552 420 394 237 127 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2551 2875 2950 3050 3791 3808 3961
Size: 1255 971 324 75 100 741 17 153
Cuts at: 3961 4146 4591 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 971 741 445 324 185 153
100 75 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4010 5722 96
Size: 274 80 33 51 1443 2033 1712 138
Fragments arranged by size:
2033 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2850 2882 5724 5756 2850
Size: 32 2842 32 2858
Fragments arranged by size:
2858 2842 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5764
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2903 2903
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 3089 1870
Size: 1219 4545
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BbeI BfrBI BplI BsiWI DraIII
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI