Restriction map for
pFN21AE3141
(Circular) (Six-base) MAPSORT of: pFN21AE3141.seq Check: 8928 from: 1 to: 5758
pFN21AE3141 5758 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3821 5205 5473 1632
Size: 2189 1384 268 1917
Fragments arranged by size:
2189 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2555 2684 1166
Size: 1389 129 4240
Fragments arranged by size:
4240 1389 129
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 2926 3501 5073 2926
Size: 575 1572 3611
Fragments arranged by size:
3611 1572 575
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 2654 2935 3067 3293 3445 5554 1493
Size: 1161 281 132 226 152 2109 1697
Fragments arranged by size:
2109 1697 1161 281 226 152 132
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 3083 1870
Size: 73 1140 4545
Fragments arranged by size:
4545 1140 73
BamHI G'GATC_C
Cuts at: 2814 2943 3088 3455 2814
Size: 129 145 367 5117
Fragments arranged by size:
5117 367 145 129
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3079 4534 618
Size: 325 217 627 1292 1455 1842
Fragments arranged by size:
1842 1455 1292 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3077 1391
Size: 1686 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4787
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2951 3650 4860 2951
Size: 699 1210 3849
Fragments arranged by size:
3849 1210 699
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4369 136
Size: 107 122 71 631 605 2697 1525
Fragments arranged by size:
2697 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 3175 3175
Size: 5758
Bme1580I G_kGCm'C
Cuts at: 1790 2268 3819 4975 1790
Size: 478 1551 1156 2573
Fragments arranged by size:
2573 1551 1156 478
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2820 3489 4447
Size: 416 53 83 399 24 1403 669 958
Cuts at: 4447 442
Size: 1753
Fragments arranged by size:
1753 1403 958 669 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2051 2243 2295 2690 2948 4418 1239
Size: 812 192 52 395 258 1470 2579
Fragments arranged by size:
2579 1470 812 395 258 192 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2933 3883 4748 5046 2933
Size: 950 865 298 3645
Fragments arranged by size:
3645 950 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2666 4421 882
Size: 617 1167 1755 2219
Fragments arranged by size:
2219 1755 1167 617
BsaAI yAC'GTr
Cuts at: 493 1204 2686 3549 493
Size: 711 1482 863 2702
Fragments arranged by size:
2702 1482 863 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2201 2945 1186
Size: 1015 744 3999
Fragments arranged by size:
3999 1015 744
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5185
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2208 3819 3904 4975 2208
Size: 1611 85 1071 2991
Fragments arranged by size:
2991 1611 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2208 2268 3077 3819 3904 4975 1391
Size: 399 418 60 809 742 85 1071 2174
Fragments arranged by size:
2174 1071 809 742 418 399 85 60
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3113 844
Size: 2269 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3645 1394
Size: 429 1822 3507
Fragments arranged by size:
3507 1822 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2725 2733 4253 4427 65
Size: 1183 1477 8 1520 174 1396
Fragments arranged by size:
1520 1477 1396 1183 174 8
BsrFI r'CCGG_y
Cuts at: 1700 1810 2186 4402 4589 1700
Size: 110 376 2216 187 2869
Fragments arranged by size:
2869 2216 376 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5758
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5758
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2557 2557
Size: 5758
BstYI r'GATC_y
Cuts at: 1972 2122 2814 2943 3088 3455 3840 3857 5309
Size: 150 692 129 145 367 385 17 1452
Cuts at: 5309 5395 5407 5753 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 692 385 367 346 150 145
129 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2648 2648
Size: 5758
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5019
Fragments arranged by size:
5019 683 56
BtsI GCAGTG_nn'
Cuts at: 3274 4068 4088 3274
Size: 794 20 4944
Fragments arranged by size:
4944 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2458 2493 529
Size: 35 1894 35 3794
Fragments arranged by size:
3794 1894 35 35
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2373 2703 2730
Size: 54 1425 50 275 18 543 330 27
Cuts at: 2730 3205 4094 5723 8
Size: 475 889 1629 43
Fragments arranged by size:
1629 1425 889 543 475 330 275 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2076 3029 3688 1353
Size: 723 953 659 3423
Fragments arranged by size:
3423 953 723 659
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4345 4719 4865 387
Size: 1064 138 2756 374 146 1280
Fragments arranged by size:
2756 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2051 2243 2295 2690 2948 3821 4418
Size: 393 419 192 52 395 258 873 597
Cuts at: 4418 5205 5473 1239
Size: 787 268 1524
Fragments arranged by size:
1524 873 787 597 419 395 393 268
258 192 52
EcoNI CCTnn'n_nnAGG
Cuts at: 2225 2828 2225
Size: 603 5155
EcoO109I rG'GnC_Cy
Cuts at: 2486 2486
Size: 5758
EcoRI G'AATT_C
Cuts at: 2935 3067 3445 2935
Size: 132 378 5248
Fragments arranged by size:
5248 378 132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2078 2363 2078
Size: 285 5473
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2283 2315 2665 2697 4399
Size: 32 135 32 711 32 350 32 1702
Cuts at: 4399 4431 4473 4505 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1702 711 350 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2611 3102 3348 669
Size: 988 281 673 491 246 3079
Fragments arranged by size:
3079 988 673 491 281 246
HindIII A'AGCT_T
Cuts at: 748 2367 748
Size: 1619 4139
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2079 2666 4872 5056 1305
Size: 774 587 2206 184 2007
Fragments arranged by size:
2206 2007 774 587 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2375 2732 10
Size: 54 1425 343 543 357 3036
Fragments arranged by size:
3036 1425 543 357 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2555 2684 3716 4075 4234 518
Size: 648 1389 129 1032 359 159 2042
Fragments arranged by size:
2042 1389 1032 648 359 159 129
MspA1I CmG'CkG
Cuts at: 1606 2147 2411 2900 2999 3174 3851 4999 5244
Size: 541 264 489 99 175 677 1148 245
Cuts at: 5244 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 541 489 264 245 175
99
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2752 3116 4661 1132
Size: 97 1523 364 1545 2229
Fragments arranged by size:
2229 1545 1523 364 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5758
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2926 1108
Size: 1818 3940
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PmlI CAC'GTG
Cuts at: 2686 2686
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3827 3859 5340 5372 3827
Size: 32 1481 32 4213
Fragments arranged by size:
4213 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2486 2486
Size: 5758
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5758
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 2574 3110 830
Size: 1744 536 3478
Fragments arranged by size:
3478 1744 536
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2147 2411 2900 2999 2147
Size: 264 489 99 4906
Fragments arranged by size:
4906 489 264 99
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 4006 5718 4006
Size: 1712 4046
SexAI A'CCwGG_T
Cuts at: 3021 3021
Size: 5758
SfcI C'TryA_G
Cuts at: 826 1045 2316 2570 2901 3106 4241 4922 5113
Size: 219 1271 254 331 205 1135 681 191
Cuts at: 5113 826
Size: 1471
Fragments arranged by size:
1471 1271 1135 681 331 254 219 205
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2948 3862 4763 5025 820
Size: 197 926 1005 914 901 262 1553
Fragments arranged by size:
1553 1005 926 914 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2416 3197 513
Size: 552 939 412 781 3074
Fragments arranged by size:
3074 939 781 552 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3066 3785 3802 3955 4140 4585 325
Size: 1255 1486 719 17 153 185 445 1498
Fragments arranged by size:
1498 1486 1255 719 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2422 4004 5716
Size: 274 80 33 51 1443 445 1582 1712
Cuts at: 5716 96
Size: 138
Fragments arranged by size:
1712 1582 1443 445 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5718 5750 5718
Size: 32 5726
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 2110 3094 2110
Size: 984 4774
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 2106 2693 3887 2106
Size: 587 1194 3977
Fragments arranged by size:
3977 1194 587
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SfoI SrfI SwaI
XcmI