Restriction map for
pFN21AE3137
(Circular) (Six-base) MAPSORT of: pFN21AE3137.seq Check: 1171 from: 1 to: 5749
pFN21AE3137 5749 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2178 3559 278
Size: 53 83 186 936 642 1381 2468
Fragments arranged by size:
2468 1381 936 642 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2493 3092 1937
Size: 556 599 4594
Fragments arranged by size:
4594 599 556
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 3812 5196 5464 1632
Size: 714 1466 1384 268 1917
Fragments arranged by size:
1917 1466 1384 714 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 2187 4648 5625 1203
Size: 984 2461 977 1327
Fragments arranged by size:
2461 1327 984 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2251 2593 3492 5064 2251
Size: 342 899 1572 2936
Fragments arranged by size:
2936 1572 899 342
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2289 4303 160
Size: 872 1257 2014 1606
Fragments arranged by size:
2014 1606 1257 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 3074 1870
Size: 73 1131 4545
Fragments arranged by size:
4545 1131 73
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2218 3070 4525 618
Size: 325 217 627 431 852 1455 1842
Fragments arranged by size:
1842 1455 852 627 431 325 217
BanII G_rGCy'C
Cuts at: 1391 3068 1391
Size: 1677 4072
BbeI G_GCGC'C
Cuts at: 2222 2222
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2015 928
Size: 971 116 4662
Fragments arranged by size:
4662 971 116
BbvCI CC'TCA_GC
Cuts at: 2766 2766
Size: 5749
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2046 2079 2080 2113 2682 2716 3940
Size: 34 109 33 1 33 569 34 1224
Cuts at: 3940 3974 1903
Size: 34 3678
Fragments arranged by size:
3678 1224 569 109 34 34 34 33
33 1
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 2833 1548
Size: 1285 4464
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2368 5744 2368
Size: 3376 2373
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 3810 4966 1790
Size: 2020 1156 2573
Fragments arranged by size:
2573 2020 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2392 3480 4438
Size: 416 53 83 399 24 975 1088 958
Cuts at: 4438 442
Size: 1753
Fragments arranged by size:
1753 1088 975 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2971 4409 1239
Size: 1732 1438 2579
Fragments arranged by size:
2579 1732 1438
Bpu10I CC'TnA_GC
Cuts at: 2766 2766
Size: 5749
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2573 3874 4739 5037 2573
Size: 1301 865 298 3285
Fragments arranged by size:
3285 1301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2549 2597 4412 882
Size: 617 1050 48 1815 2219
Fragments arranged by size:
2219 1815 1050 617 48
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 2367 5743 2367
Size: 3376 2373
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 2219 3556 3938
Size: 53 83 186 936 642 44 1337 382
Cuts at: 3938 4573 275
Size: 635 1451
Fragments arranged by size:
1451 1337 936 642 635 382 186 83
53 44
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5749
BseYI C'CCAG_C
Cuts at: 4952 5448 4952
Size: 496 5253
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3004 1163
Size: 573 1268 3908
Fragments arranged by size:
3908 1268 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2216 3199 3960 4988 1540
Size: 275 186 215 983 761 1028 2301
Fragments arranged by size:
2301 1028 983 761 275 215 186
BsiHKAI G_wGCw'C
Cuts at: 3044 3810 3895 4966 3044
Size: 766 85 1071 3827
Fragments arranged by size:
3827 1071 766 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3044 3068 3810 3895 4966 1391
Size: 399 1254 24 742 85 1071 2174
Fragments arranged by size:
2174 1254 1071 742 399 85 24
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 3638 5368 3638
Size: 1730 4019
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2360 3104 844
Size: 1516 744 3489
Fragments arranged by size:
3489 1516 744
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2974 4244 4418 65
Size: 1183 1726 1270 174 1396
Fragments arranged by size:
1726 1396 1270 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2033 2124 2215 4393 4580 1700
Size: 110 223 91 91 2178 187 2869
Fragments arranged by size:
2869 2178 223 187 110 91 91
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 2108 2110 1925
Size: 183 2 5564
Fragments arranged by size:
5564 183 2
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2229 2229
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2368 3079 3446 3831 3848 5300 5386 5398
Size: 396 711 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 711 396 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2048 2085 513
Size: 552 983 37 4177
Fragments arranged by size:
4177 983 552 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 2591 2736 3017 3265 4059 4079 2591
Size: 145 281 248 794 20 4261
Fragments arranged by size:
4261 794 281 248 145 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2575 3196 4085
Size: 54 1425 50 275 18 745 621 889
Cuts at: 4085 5714 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 745 621 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3023 3679 1353
Size: 1670 656 3423
Fragments arranged by size:
3423 1670 656
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2971 3812 4409 5196 5464 1239
Size: 393 714 625 841 597 787 268 1524
Fragments arranged by size:
1524 841 787 714 625 597 393 268
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2222 4896 1678
Size: 544 2674 2531
Fragments arranged by size:
2674 2531 544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2384 2416 2475 2507 2842
Size: 32 135 32 812 32 59 32 335
Cuts at: 2842 2874 4390 4422 4464 4496 1373
Size: 32 1516 32 42 32 2626
Fragments arranged by size:
2626 1516 812 335 135 59 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2218 2218
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 2187 5625 2187
Size: 3438 2311
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4863 5047 1305
Size: 3558 184 2007
Fragments arranged by size:
3558 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2577 10
Size: 54 1425 343 745 3182
Fragments arranged by size:
3182 1425 745 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2227 3707 4066 4225 518
Size: 648 1061 1480 359 159 2042
Fragments arranged by size:
2042 1480 1061 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2050 2087 2500 3165 3842 4990 5235 1606
Size: 444 37 413 665 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 665 444 413 245 37
NarI GG'CG_CC
Cuts at: 2219 2219
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2697 3107 4652 1132
Size: 97 1468 410 1545 2229
Fragments arranged by size:
2229 1545 1468 410 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 2348 3319 2348
Size: 971 4778
PstI C_TGCA'G
Cuts at: 830 2133 2386 3101 830
Size: 1303 253 715 3478
Fragments arranged by size:
3478 1303 715 253
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2500 2500
Size: 5749
SacII CC_GC'GG
Cuts at: 2051 2088 2051
Size: 37 5712
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SfcI C'TryA_G
Cuts at: 826 1045 2129 2382 3097 4232 4913 5104 826
Size: 219 1084 253 715 1135 681 191 1471
Fragments arranged by size:
1471 1135 1084 715 681 253 219 191
SfoI GGC'GCC
Cuts at: 2220 2220
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 3076 1872
Size: 1204 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2552 3853 4754 5016 820
Size: 197 926 609 1301 901 262 1553
Fragments arranged by size:
1553 1301 926 901 609 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 2513 3673 5
Size: 47 2461 1160 2081
Fragments arranged by size:
2461 2081 1160 47
StuI AGG'CCT
Cuts at: 1517 2061 1517
Size: 544 5205
StyI C'CwwG_G
Cuts at: 513 1065 3188 513
Size: 552 2123 3074
Fragments arranged by size:
3074 2123 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2283 2329 3776 3793 3946 4131 4576
Size: 1255 703 46 1447 17 153 185 445
Cuts at: 4576 325
Size: 1498
Fragments arranged by size:
1498 1447 1255 703 445 185 153 46
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5709 5741 5709
Size: 32 5717
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 2432 3085 2432
Size: 653 5096
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 3074 1870
Size: 1204 4545
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2176 3557 276
Size: 53 83 186 936 642 1381 2468
Fragments arranged by size:
2468 1381 936 642 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SapI SexAI SfiI SrfI SwaI
XcmI